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P01521

- CA1_CONMA

UniProt

P01521 - CA1_CONMA

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Protein
Alpha-conotoxin MI
Gene
N/A
Organism
Conus magus (Magus cone) (Magician's cone snail)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Alpha-conotoxins act on postsynaptic membranes, they bind to the nicotinic acetylcholine receptors (nAChR) and thus inhibit them. Blocks mammalian nAChR composed of alpha-1/delta subunits with high potency and alpha-1/gamma with a low potency.1 Publication

Keywords - Molecular functioni

Acetylcholine receptor inhibiting toxin, Ion channel impairing toxin, Neurotoxin, Postsynaptic neurotoxin, Toxin

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-conotoxin MI
Short name:
Alpha-MI
Short name:
CtxMI
Alternative name(s):
M1
OrganismiConus magus (Magus cone) (Magician's cone snail)
Taxonomic identifieri6492 [NCBI]
Taxonomic lineageiEukaryotaMetazoaLophotrochozoaMolluscaGastropodaCaenogastropodaHypsogastropodaNeogastropodaConoideaConidaeConus

Organism-specific databases

ConoServeri23. MI.

Subcellular locationi

GO - Cellular componenti

  1. other organism postsynaptic membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi2 – 21R → A: 4-fold loss of activity. 1 Publication
Mutagenesisi5 – 51H → A: 3-fold loss of activity. 1 Publication
Mutagenesisi6 – 61P → A: 73-fold loss of activity. 1 Publication
Mutagenesisi10 – 101K → A: 9-fold loss of activity. 1 Publication
Mutagenesisi11 – 111N → A: 3-fold loss of activity. 1 Publication
Mutagenesisi12 – 121Y → A: 8500-fold loss of activity. 1 Publication
Mutagenesisi12 – 121Y → H: 33-fold loss of activity. 1 Publication
Mutagenesisi12 – 121Y → M: 48-fold loss of activity. 1 Publication
Mutagenesisi12 – 121Y → W: 5-fold loss of activity. 1 Publication
Mutagenesisi13 – 131S → A: No loss of activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Peptidei1 – 1414Alpha-conotoxin MI
PRO_0000044461Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi3 ↔ 81 Publication
Disulfide bondi4 ↔ 141 Publication
Modified residuei14 – 141Cysteine amide1 Publication

Post-translational modificationi

Amidated; synthetic peptide with a C-terminus free is 6-fold less active than the amidated peptide.

Keywords - PTMi

Amidation, Disulfide bond

Expressioni

Tissue specificityi

Expressed by the venom duct.

Family & Domainsi

Domaini

The cysteine framework is I (CC-C-C). Alpha3/5 pattern.

Sequence similaritiesi

Belongs to the conotoxin A superfamily.

Family and domain databases

InterProiIPR018072. Conotoxin_a-typ_CS.
[Graphical view]
PROSITEiPS60014. ALPHA_CONOTOXIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P01521-1 [UniParc]FASTAAdd to Basket

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GRCCHPACGK NYSC                                          14
Length:14
Mass (Da):1,499
Last modified:July 21, 1986 - v1
Checksum:iDEEE91898BF5E5BD
GO

Sequence databases

PIRiA01784. NTKN1M.

Cross-referencesi

Sequence databases

PIRi A01784. NTKN1M.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Organism-specific databases

ConoServeri 23. MI.

Family and domain databases

InterProi IPR018072. Conotoxin_a-typ_CS.
[Graphical view ]
PROSITEi PS60014. ALPHA_CONOTOXIN. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Isolation and structure of a peptide toxin from the marine snail Conus magus."
    McIntosh J.M., Cruz L.J., Hunkapiller M.W., Gray W.R., Olivera B.M.
    Arch. Biochem. Biophys. 218:329-334(1982) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE.
  2. "Conotoxin MI. Disulfide bonding and conformational states."
    Gray W.R., Rivier J.E., Galyean R., Cruz L.J., Olivera B.M.
    J. Biol. Chem. 258:12247-12251(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISULFIDE BONDS.
  3. "Critical residues influence the affinity and selectivity of alpha-conotoxin MI for nicotinic acetylcholine receptors."
    Jacobsen R.B., DelaCruz R.G., Grose J.H., McIntosh J.M., Yoshikami D., Olivera B.M.
    Biochemistry 38:13310-13315(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SYNTHESIS, MUTAGENESIS OF ARG-2; HIS-5; PRO-6; LYS-10; ASN-11; TYR-12 AND SER-13.

Entry informationi

Entry nameiCA1_CONMA
AccessioniPrimary (citable) accession number: P01521
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: September 3, 2014
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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