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P01519

- CA1A_CONGE

UniProt

P01519 - CA1A_CONGE

Protein

Alpha-conotoxin GIA

Gene
N/A
Organism
Conus geographus (Geography cone) (Nubecula geographus)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Alpha-conotoxins act on postsynaptic membranes, they bind to the nicotinic acetylcholine receptors (nAChR) and thus inhibit them. The higher affinity site for alpha-conotoxin GI is the alpha/delta site on mouse muscle-derived BC3H-1 receptor, and the other site (alpha/gamma site) on nicotinic receptors from Torpedo californica electric organ.

    Keywords - Molecular functioni

    Acetylcholine receptor inhibiting toxin, Ion channel impairing toxin, Neurotoxin, Postsynaptic neurotoxin, Toxin

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alpha-conotoxin GIA
    Cleaved into the following chain:
    Alternative name(s):
    G1
    OrganismiConus geographus (Geography cone) (Nubecula geographus)
    Taxonomic identifieri6491 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaLophotrochozoaMolluscaGastropodaCaenogastropodaHypsogastropodaNeogastropodaConoideaConidaeConus

    Organism-specific databases

    ConoServeri74. GI.
    22. GIA.

    Subcellular locationi

    GO - Cellular componenti

    1. other organism postsynaptic membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Secreted

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi9 – 91R → A: Reduction in affinity for both alpha/delta and alpha/gamma sites on BC3H-1 receptors and loss of affinity for both alpha/delta and alpha/gamma sites on Torpedo receptors (in GI). 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Peptidei1 – 1515Alpha-conotoxin GIAPRO_0000034873Add
    BLAST
    Peptidei1 – 1313Alpha-conotoxin GIPRO_0000034874Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi2 ↔ 7
    Disulfide bondi3 ↔ 13
    Modified residuei13 – 131Cysteine amide; in alpha-conotoxin GI
    Modified residuei15 – 151Lysine amide; in form alpha-conotoxin GIA

    Post-translational modificationi

    Not hydroxylated; hydroxylation, on a synthetic hydroxylated GI, improves its folding but impairs its activity against target receptors.

    Keywords - PTMi

    Amidation, Disulfide bond

    Expressioni

    Tissue specificityi

    Expressed by the venom duct.

    Structurei

    Secondary structure

    1
    15
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi5 – 106

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1NOTX-ray1.20A1-13[»]
    1QS3NMR-A2-12[»]
    1XGANMR-A1-13[»]
    1XGBNMR-A1-13[»]
    1XGCNMR-A1-13[»]
    2FR9NMR-A1-11[»]
    2FRBNMR-A1-13[»]
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP01519.

    Family & Domainsi

    Domaini

    The cysteine framework is I (CC-C-C). Alpha3/5 pattern.

    Sequence similaritiesi

    Belongs to the conotoxin A superfamily.Curated

    Family and domain databases

    InterProiIPR018072. Conotoxin_a-typ_CS.
    [Graphical view]
    PROSITEiPS60014. ALPHA_CONOTOXIN. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P01519-1 [UniParc]FASTAAdd to Basket

    « Hide

    ECCNPACGRH YSCGK                                         15
    Length:15
    Mass (Da):1,628
    Last modified:July 21, 1986 - v1
    Checksum:i2AE73EE90F8C2E19
    GO

    Sequence databases

    PIRiA01782. NTKNAG.

    Cross-referencesi

    Sequence databases

    PIRi A01782. NTKNAG.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1NOT X-ray 1.20 A 1-13 [» ]
    1QS3 NMR - A 2-12 [» ]
    1XGA NMR - A 1-13 [» ]
    1XGB NMR - A 1-13 [» ]
    1XGC NMR - A 1-13 [» ]
    2FR9 NMR - A 1-11 [» ]
    2FRB NMR - A 1-13 [» ]
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Organism-specific databases

    ConoServeri 74. GI.
    22. GIA.

    Miscellaneous databases

    EvolutionaryTracei P01519.

    Family and domain databases

    InterProi IPR018072. Conotoxin_a-typ_CS.
    [Graphical view ]
    PROSITEi PS60014. ALPHA_CONOTOXIN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Peptide toxins from Conus geographus venom."
      Gray W.R., Luque A., Olivera B.M., Barrett J., Cruz L.J.
      J. Biol. Chem. 256:4734-4740(1981) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE.
    2. "Primary and secondary structure of conotoxin GI, a neurotoxic tridecapeptide from a marine snail."
      Nishiuchi Y., Sakakibara S.
      FEBS Lett. 148:260-262(1982) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISULFIDE BONDS IN GI, SYNTHESIS OF GI.
    3. "Conotoxin GI: disulfide bridges, synthesis, and preparation of iodinated derivatives."
      Gray W.R., Luque F.A., Galyean R., Atherton E., Sheppard R.C., Stone B.L., Reyes A., Alford J., McIntosh M., Olivera B.M., Cruz L.J., Rivier J.
      Biochemistry 23:2796-2802(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISULFIDE BONDS IN GI, SYNTHESIS OF GI.
    4. "The alpha-conotoxins GI and MI distinguish between the nicotinic acetylcholine receptor agonist sites while SI does not."
      Hann R.M., Pagan O.R., Eterovic V.A.
      Biochemistry 33:14058-14063(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: COMPARISON WITH ALPHA-CONOTOXIN SI AND ALPHA-CONOTOXIN MI.
    5. "Alpha-conotoxins selectively inhibit one of the two acetylcholine binding sites of nicotinic receptors."
      Groebe D.R., Dumm J.M., Levitan E.S., Abramson S.N.
      Mol. Pharmacol. 48:105-111(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHARMACOLOGICAL CHARACTERIZATION ON MOUSE MUSCLE-DERIVED BC3H-1 CELLS AND TORPEDO ELECTRIC ORGAN.
    6. "Determinants involved in the affinity of alpha-conotoxins GI and SI for the muscle subtype of nicotinic acetylcholine receptors."
      Groebe D.R., Gray W.R., Abramson S.N.
      Biochemistry 36:6469-6474(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: MUTAGENESIS OF ARG-9.
    7. "Role of hydroxyprolines in the in vitro oxidative folding and biological activity of conotoxins."
      Lopez-Vera E., Walewska A., Skalicky J.J., Olivera B.M., Bulaj G.
      Biochemistry 47:1741-1751(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: SYNTHESIS, ROLE OF HYDROXYLATION.
    8. "Three-dimensional structure of the alpha-conotoxin GI at 1.2-A resolution."
      Guddat L.W., Martin J.A., Shan L., Edmundson A.B., Gray W.R.
      Biochemistry 35:11329-11335(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.2 ANGSTROMS) OF GI, DISULFIDE BONDS.
    9. "Solution conformation of conotoxin GI determined by 1H nuclear magnetic resonance spectroscopy and distance geometry calculations."
      Kobayashi Y., Ohkubo T., Kyogoku Y., Nishiuchi Y., Sakakibara S., Braun W., Go N.
      Biochemistry 28:4853-4860(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR OF GI, DISULFIDE BONDS.
    10. "Solution structures of alpha-conotoxin G1 determined by two-dimensional NMR spectroscopy."
      Pardi A., Galdes A., Florance J., Maniconte D.
      Biochemistry 28:5494-5501(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR OF GI, DISULFIDE BONDS.
    11. Cited for: STRUCTURE BY NMR OF GI, DISULFIDE BONDS.
    12. "Structure determination of the three disulfide bond isomers of alpha-conotoxin GI: a model for the role of disulfide bonds in structural stability."
      Gehrmann J., Alewood P.F., Craik D.J.
      J. Mol. Biol. 278:401-415(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR OF GI, DISULFIDE BONDS.
    13. "NMR solution conformation of an antitoxic analogue of alpha-conotoxin GI: identification of a common nicotinic acetylcholine receptor alpha(1)-subunit binding surface for small ligands and alpha-conotoxins."
      Mok K.H., Han K.H.
      Biochemistry 38:11895-11904(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR OF AN ANTITOXIC ANALOG OF GI, DISULFIDE BONDS.

    Entry informationi

    Entry nameiCA1A_CONGE
    AccessioniPrimary (citable) accession number: P01519
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: July 21, 1986
    Last modified: October 1, 2014
    This is version 93 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programAnimal Toxin Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Direct protein sequencing

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3