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P01500 (APAM_APIME) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 78. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
Apamin
OrganismApis mellifera (Honeybee) [Reference proteome]
Taxonomic identifier7460 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaHymenopteraApocritaAculeataApoideaApidaeApis

Protein attributes

Sequence length46 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Neurotoxin that blocks voltage-independent calcium-activated potassium channels (KCNN1=SK1, KCNN2=SK2, KCNN3=SK3).

Subcellular location

Secreted.

Tissue specificity

Expressed by the venom gland.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2727 Ref.2 Ref.3
Peptide28 – 4518Apamin Ref.2 Ref.3
PRO_0000018611

Regions

Region40 – 412Essential for toxin activity

Amino acid modifications

Modified residue451Histidine amide
Disulfide bond28 ↔ 38
Disulfide bond30 ↔ 42

Sequences

Sequence LengthMass (Da)Tools
P01500 [UniParc].

Last modified October 1, 1996. Version 2.
Checksum: 92694A07501AEE33

FASTA465,223
        10         20         30         40 
MISMLRCIYL FLSVILITSY FVTPVMPCNC KAPETALCAR RCQQHG 

« Hide

References

[1]"The precursors of the bee venom constituents apamin and MCD peptide are encoded by two genes in tandem which share the same 3'-exon."
Gmachl M., Kreil G.
J. Biol. Chem. 270:12704-12708(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Venom gland.
[2]"Sequence analysis of bee venom neurotoxin (apamine) from its tryptic and chymotryptic cleavage products."
Haux P., Sawerthal H., Habermann E.
Hoppe-Seyler's Z. Physiol. Chem. 348:737-738(1967) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 28-45.
Tissue: Venom.
[3]Shipolini R., Bradbury A.F., Callewaert G.L., Vernon C.A.
J. Chem. Soc. Chem. Commun. 1967:679-680(1967)
Cited for: PROTEIN SEQUENCE OF 28-45.
Tissue: Venom.
[4]"Solution structure of apamin determined by nuclear magnetic resonance and distance geometry."
Pease J.H.B., Wemmer D.E.
Biochemistry 27:8491-8498(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY NMR OF 28-45.
[5]"Spatial structure of apamin in solution."
Andrianov A.M., Akhrem A.A.
Mol. Biol. (Mosk.) 25:937-945(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY NMR OF 28-45.
[6]"Binding and toxicity of apamin. Characterization of the active site."
Labee-Jullie C., Granier C., Alberico F., Defendini M.-L., Ceard B., Rochat H., van Rietschoten J.
Eur. J. Biochem. 196:639-645(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: SYNTHESIS, ACTIVITY OF ANALOGS.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
S78458 mRNA. Translation: AAB34402.1.
PIRAMHB. A56710.
RefSeqNP_001011612.1. NM_001011612.1.
UniGeneAme.1216.

3D structure databases

ProteinModelPortalP01500.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING7460.P01500.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaGB18161-RA; GB18161-PA; GB18161.
GeneID406135.
KEGGame:406135.

Organism-specific databases

CTD406135.

Phylogenomic databases

HOGENOMHOG000033980.
OMANCKAPET.

Family and domain databases

ProtoNetSearch...

Entry information

Entry nameAPAM_APIME
AccessionPrimary (citable) accession number: P01500
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: October 1, 1996
Last modified: May 14, 2014
This is version 78 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program