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P01497

- SIX1_ANDAU

UniProt

P01497 - SIX1_ANDAU

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Protein

Beta-insect excitatory toxin 1

Gene
N/A
Organism
Androctonus australis (Sahara scorpion)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Excitatory insect beta-toxins induce a spastic paralysis. They bind voltage-independently at site-4 of sodium channels (Nav) and shift the voltage of activation toward more negative potentials thereby affecting sodium channel activation and promoting spontaneous and repetitive firing. This toxin is active only on insects.

GO - Molecular functioni

  1. ion channel inhibitor activity Source: InterPro

GO - Biological processi

  1. defense response Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Ion channel impairing toxin, Neurotoxin, Toxin, Voltage-gated sodium channel impairing toxin

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-insect excitatory toxin 1
Alternative name(s):
AaH IT1
Short name:
AaH IT
Short name:
AaHIT
Short name:
AaHIT1
Short name:
AaIT1
OrganismiAndroctonus australis (Sahara scorpion)
Taxonomic identifieri6858 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaChelicerataArachnidaScorpionesButhidaButhoideaButhidaeAndroctonus

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Toxic dosei

PD50 is 0.099 ng/mg of insects.1 Publication

Biotechnological usei

Could be used to reinforce the natural insecticidal ability of baculoviruses. The insertion of the gene coding for AaHIT causes a higher killing speed of insects by the baculovirus (particularly against lepidopterans).1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 18182 PublicationsAdd
BLAST
Chaini19 – 8870Beta-insect excitatory toxin 1PRO_0000035187Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi34 ↔ 551 Publication
Disulfide bondi40 ↔ 601 Publication
Disulfide bondi44 ↔ 621 Publication
Disulfide bondi56 ↔ 821 Publication

Keywords - PTMi

Disulfide bond

Expressioni

Tissue specificityi

Expressed by the venom gland.

Structurei

3D structure databases

ProteinModelPortaliP01497.
SMRiP01497. Positions 19-88.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.30.30.10. 1 hit.
InterProiIPR003614. Scorpion_toxin-like.
IPR002061. Scorpion_toxinL/defensin.
[Graphical view]
PfamiPF00537. Toxin_3. 1 hit.
[Graphical view]
SMARTiSM00505. Knot1. 1 hit.
[Graphical view]
SUPFAMiSSF57095. SSF57095. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P01497-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKFLLLFLVV LPIMGVFGKK NGYAVDSSGK APECLLSNYC NNECTKVHYA
60 70 80
DKGYCCLLSC YCFGLNDDKK VLEISDTRKS YCDTTIIN
Length:88
Mass (Da):9,852
Last modified:July 21, 1986 - v2
Checksum:iE6FF097B3277748F
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti43 – 431E → Q AA sequence 1 PublicationCurated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti17 – 171F → L.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M27706 mRNA. Translation: AAA29951.1.
M27705 mRNA. Translation: AAA29950.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M27706 mRNA. Translation: AAA29951.1 .
M27705 mRNA. Translation: AAA29950.1 .

3D structure databases

ProteinModelPortali P01497.
SMRi P01497. Positions 19-88.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.30.30.10. 1 hit.
InterProi IPR003614. Scorpion_toxin-like.
IPR002061. Scorpion_toxinL/defensin.
[Graphical view ]
Pfami PF00537. Toxin_3. 1 hit.
[Graphical view ]
SMARTi SM00505. Knot1. 1 hit.
[Graphical view ]
SUPFAMi SSF57095. SSF57095. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Precursors of Androctonus australis scorpion neurotoxins. Structures of precursors, processing outcomes, and expression of a functional recombinant toxin II."
    Bougis P.E., Rochat H., Smith L.A.
    J. Biol. Chem. 264:19259-19265(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Hector.
  2. "Neurotoxins active on insects: amino acid sequences, chemical modifications, and secondary structure estimation by circular dichroism of toxins from the scorpion Androctonus australis hector."
    Loret E.P., Mansuelle P., Rochat H., Granier C.
    Biochemistry 29:1492-1501(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 19-88, PARALYTIC DOSE.
    Strain: Hector.
    Tissue: Venom.
  3. "Covalent structure of the insect toxin of Androctonus australis hector."
    Darbon H., Rochat H., Kopeyan C., van Rietschoten J., Zlotkin E.
    Toxicon 20:64-64(1982)
    Cited for: PROTEIN SEQUENCE OF 19-88.
    Strain: Hector.
    Tissue: Venom.
  4. Cited for: BIOTECHNOLOGY, REVIEW.
  5. "Two-dimensional 1H nuclear magnetic resonance study of AaH IT, an anti-insect toxin from the scorpion Androctonus australis hector. Sequential resonance assignments and folding of the polypeptide chain."
    Darbon H., Weber C., Braun W.
    Biochemistry 30:1836-1845(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR, DISULFIDE BONDS.
    Strain: Hector.

Entry informationi

Entry nameiSIX1_ANDAU
AccessioniPrimary (citable) accession number: P01497
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: October 29, 2014
This is version 91 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3