P01490 (SCXA_MESEU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Alpha-toxin BeM10 Alternative name(s): Neurotoxin M10
Organism	Mesobuthus eupeus (Lesser Asian scorpion) (Buthus eupeus)
Taxonomic identifier	34648 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Chelicerata › Arachnida › Scorpiones › Buthida › Buthoidea › Buthidae › Mesobuthus

Protein attributes

Sequence length	65 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Alpha toxins bind voltage-independently at site-3 of sodium channels (Nav) and inhibit the inactivation of the activated channels, thereby blocking neuronal transmission. Has paralytic activity in mice.
Subcellular location	Secreted.
Tissue specificity	Expressed by the venom gland.
Sequence similarities	Belongs to the long (4 C-C) scorpion toxin superfamily. Sodium channel inhibitor family. Alpha subfamily.

Ontologies

Keywords
Cellular component	Secreted
Molecular function	Ion channel impairing toxin Neurotoxin Sodium channel inhibitor Toxin
PTM	Disulfide bond
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	defense response Inferred from electronic annotation. Source: InterPro
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	sodium channel inhibitor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 65	65	Alpha-toxin BeM10		PRO_0000066730
Amino acid modifications
	Disulfide bond	12 ↔ 64		By similarity
	Disulfide bond	16 ↔ 35		By similarity
	Disulfide bond	22 ↔ 45		By similarity
	Disulfide bond	26 ↔ 47		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P01490 [UniParc]. Last modified July 21, 1986. Version 1. Checksum: E79FFE5DEE6CB694	FASTA	65	7,373
	10 20 30 40 50 60 VRDGYIADDK DCAYFCGRNA YCDEECKKGA ESGKCWYAGQ YGNACWCYKL PDWVPIKQKV SGKCN

References

[1]	"Studies of the toxins from Buthus eupeus scorpion venom." Grishin E.V., Soldatov N.M., Soldatova L.N., Ovchinnikov Y.A. Toxicon 17 Suppl. 1:60-60(1979) Cited for: PROTEIN SEQUENCE. Tissue: Venom.
[2]	"The amino acid sequence of neurotoxin M10 from the venom of the Central Asian scorpion Buthus eupeus." Grishin E.V., Soldatova L.N., Shakhparonov M.I., Kazakov V.K. Bioorg. Khim. 6:714-723(1980) Cited for: PROTEIN SEQUENCE. Tissue: Venom.
[3]	"Neurotoxins from the venom of the Central Asian scorpion Buthus eupeus." Volkova T.M., Garsia A.F., Telezhinskaia I.N., Potapenko N.A., Grishin E.V. Bioorg. Khim. 11:1445-1456(1985) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE. Tissue: Venom.

Cross-references

Sequence databases
PIR	NTSR0E. JT0019.
3D structure databases
ProteinModelPortal	P01490.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	3.30.30.10. 1 hit.
InterPro	IPR003614. Scorpion_toxin-like. IPR018218. Scorpion_toxinL. IPR002061. Scorpion_toxinL/defesin. [Graphical view]
Pfam	PF00537. Toxin_3. 1 hit. [Graphical view]
PRINTS	PR00285. SCORPNTOXIN.
SMART	SM00505. Knot1. 1 hit. [Graphical view]
SUPFAM	SSF57095. SSF57095. 1 hit.
ProtoNet	Search...

Entry information

Entry name

SCXA_MESEU

Accession

Primary (citable) accession number: P01490

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	May 1, 2013
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Animal Toxin Annotation Program