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Protein

Alpha-mammal toxin AaH2

Gene
N/A
Organism
Androctonus australis (Sahara scorpion)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Alpha toxins bind voltage-independently at site-3 of sodium channels (Nav) and inhibit the inactivation of the activated channels, thereby blocking neuronal transmission. This toxin is active against mammals.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ion channel impairing toxin, Neurotoxin, Toxin, Voltage-gated sodium channel impairing toxin

Protein family/group databases

TCDBi8.B.1.1.7. the long (4c-c) scorpion toxin (l-st) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-mammal toxin AaH2
Alternative name(s):
AaH II
Short name:
AaHII
Neurotoxin 2
OrganismiAndroctonus australis (Sahara scorpion)
Taxonomic identifieri6858 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaChelicerataArachnidaScorpionesButhidaButhoideaButhidaeAndroctonus

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Toxic dosei

LD50 is 25 ng/kg by intracerebroventricular injection into mice, and LD50 is 11 µg/kg by subcutaneous injection.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi27 – 293DDV → KNY: Active on insects, but not on mammals; when associated with F-36 and 75-P--K-81. 1 Publication
Mutagenesisi36 – 361G → F: Active on insects, but not on mammals; when associated with 27-K--Y-29 and 75-P--K-81. 1 Publication
Mutagenesisi75 – 817RTKGPGR → PIRVPGK: Active on insects, but not on mammals; when associated with 27-K--Y-29 and F-36. 1 Publication
Mutagenesisi77 – 771K → E, I or V: Loss of toxin toxicity; tested only without C-terminal amidation. 1 Publication
Mutagenesisi83 – 831H → HG: Little loss in toxicity; G-84 is not amidated. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 19191 PublicationAdd
BLAST
Chaini20 – 8364Alpha-mammal toxin AaH2PRO_0000035220Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi31 ↔ 822 Publications
Disulfide bondi35 ↔ 552 Publications
Disulfide bondi41 ↔ 652 Publications
Disulfide bondi45 ↔ 672 Publications
Modified residuei83 – 831Histidine amide

Post-translational modificationi

The amidation of His-83 is not necessary for toxicity.

Keywords - PTMi

Amidation, Disulfide bond

Expressioni

Tissue specificityi

Expressed by the venom gland.

Structurei

Secondary structure

1
85
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi21 – 266Combined sources
Turni28 – 303Combined sources
Helixi38 – 4710Combined sources
Beta strandi51 – 599Combined sources
Beta strandi62 – 709Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1AHOX-ray0.96A20-83[»]
1PTXX-ray1.30A20-83[»]
1SEGX-ray1.30A20-83[»]
4AEIX-ray2.30A/B/C20-83[»]
ProteinModelPortaliP01484.
SMRiP01484. Positions 20-83.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP01484.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.30.30.10. 1 hit.
InterProiIPR003614. Scorpion_toxin-like.
IPR018218. Scorpion_toxinL.
IPR002061. Scorpion_toxinL/defensin.
[Graphical view]
PfamiPF00537. Toxin_3. 1 hit.
[Graphical view]
PRINTSiPR00285. SCORPNTOXIN.
SMARTiSM00505. Knot1. 1 hit.
[Graphical view]
SUPFAMiSSF57095. SSF57095. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P01484-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNYLVMISLA LLFVTGVESV KDGYIVDDVN CTYFCGRNAY CNEECTKLKG
60 70 80
ESGYCQWASP YGNACYCYKL PDHVRTKGPG RCHGR
Length:85
Mass (Da):9,548
Last modified:July 1, 1993 - v3
Checksum:i1740CC6B98363768
GO

Mass spectrometryi

Molecular mass is 7243.20 Da from positions 20 - 83. Determined by ESI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M27704 mRNA. Translation: AAA29949.1.
PIRiD34444. NTSR2A.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M27704 mRNA. Translation: AAA29949.1.
PIRiD34444. NTSR2A.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1AHOX-ray0.96A20-83[»]
1PTXX-ray1.30A20-83[»]
1SEGX-ray1.30A20-83[»]
4AEIX-ray2.30A/B/C20-83[»]
ProteinModelPortaliP01484.
SMRiP01484. Positions 20-83.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

TCDBi8.B.1.1.7. the long (4c-c) scorpion toxin (l-st) superfamily.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP01484.

Family and domain databases

Gene3Di3.30.30.10. 1 hit.
InterProiIPR003614. Scorpion_toxin-like.
IPR018218. Scorpion_toxinL.
IPR002061. Scorpion_toxinL/defensin.
[Graphical view]
PfamiPF00537. Toxin_3. 1 hit.
[Graphical view]
PRINTSiPR00285. SCORPNTOXIN.
SMARTiSM00505. Knot1. 1 hit.
[Graphical view]
SUPFAMiSSF57095. SSF57095. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiSCX2_ANDAU
AccessioniPrimary (citable) accession number: P01484
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 1, 1993
Last modified: December 9, 2015
This is version 116 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program

Miscellaneousi

Miscellaneous

EC(50) is 2.6 nM on rat brain Nav1.2 (SCN2A) sodium channel. EC(50) is 2.2 nM on rat skeletal muscle Nav1.4 (SCN4A) sodium channel.
The chimeric protein obtained (PubMed:15133045) with the three mutants 27-Asp--Val-29, Gly-36 and 75-Arg--Arg-81 is termed chimera Aah2/Lqh-alpha-IT(27-29,36,75-81). This chimera was constructed so as to test the importance of critical residues of Lqh-alpha-IT.1 Publication

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.