P01479 (SCX1_ANDAU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Neurotoxin-1'' Alternative name(s): AaH I'' Short name=AaHI'' Neurotoxin I'' Cleaved into the following chain: Neurotoxin-1/1' Alternative name(s): AaH I/AaH I' Short name=AaHI/AaHI' Neurotoxin I/I'
Organism	Androctonus australis (Sahara scorpion)
Taxonomic identifier	6858 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Chelicerata › Arachnida › Scorpiones › Buthida › Buthoidea › Buthidae › Androctonus

Protein attributes

Sequence length	83 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Alpha toxins bind voltage-independently at site-3 of sodium channels (Nav) and inhibit the inactivation of the activated channels, thereby blocking neuronal transmission. Is active against mammals and binds with high affinity rat brain synaptosomes.
Subcellular location	Secreted.
Tissue specificity	Expressed by the venom gland.
Sequence similarities	Belongs to the long (4 C-C) scorpion toxin superfamily. Sodium channel inhibitor family. Alpha subfamily.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Ion channel impairing toxin Neurotoxin Sodium channel inhibitor Toxin
PTM	Disulfide bond
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	defense response Inferred from electronic annotation. Source: InterPro
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	sodium channel inhibitor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	Ref.3 Ref.4
	Chain	20 – 83	64	Neurotoxin-1''		PRO_0000035217
	Chain	20 – 82	63	Neurotoxin-1/1'		PRO_0000035218
	Propeptide	83	1	Removed by a carboxypeptidase (in neurotoxin-1/1')		PRO_0000035219
Amino acid modifications
	Disulfide bond	31 ↔ 81		By similarity
	Disulfide bond	35 ↔ 53		By similarity
	Disulfide bond	39 ↔ 63		By similarity
	Disulfide bond	43 ↔ 65		By similarity
Natural variations
	Natural variant	36	1	V → I in neurotoxin-1'.

Sequences

	Sequence		Length	Mass (Da)
	P01479 [UniParc]. Last modified July 1, 1993. Version 3. Checksum: 7B6F72F3CB98D1C6	FASTA	83	9,061
	10 20 30 40 50 60 MNYLVMISLA LLLMIGVESK RDGYIVYPNN CVYHCVPPCD GLCKKNGGSS GSCSFLVPSG 70 80 LACWCKDLPD NVPIKDTSRK CTR

References

[1]	"Precursors of Androctonus australis scorpion neurotoxins. Structures of precursors, processing outcomes, and expression of a functional recombinant toxin II." Bougis P.E., Rochat H., Smith L.A. J. Biol. Chem. 264:19259-19265(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (AAH I AND AAH I'). Strain: Hector.
[2]	"Promoter structure and intron-exon organization of a scorpion alpha-toxin gene." Delabre M.-L., Pasero P., Marilley M., Bougis P.E. Biochemistry 34:6729-6736(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (AAH I'). Strain: Hector.
[3]	"The amino acid sequence of neurotoxin I of Androctonus australis hector." Rochat H., Rochat C., Miranda F., Lissitzky S., Edman P. Eur. J. Biochem. 17:262-266(1970) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 20-82 (AAH I). Strain: Hector. Tissue: Venom.
[4]	"Purification and amino acid sequence of toxin I' from the venom of the North African scorpion Androctonus australis hector." Martin M.-F., Rochat H. Toxicon 22:695-703(1984) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 20-83 (AAH I''). Strain: Hector. Tissue: Venom.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M27701 mRNA. Translation: AAA29946.1. M27702 mRNA. Translation: AAA29947.1. X76135 Genomic DNA. Translation: CAA53740.1.
PIR	NTSRIA. A34444. NTSRI2. B34444.
3D structure databases
ProteinModelPortal	P01479.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	3.30.30.10. 1 hit.
InterPro	IPR003614. Scorpion_toxin-like. IPR018218. Scorpion_toxinL. IPR002061. Scorpion_toxinL/defesin. [Graphical view]
Pfam	PF00537. Toxin_3. 1 hit. [Graphical view]
PRINTS	PR00285. SCORPNTOXIN.
SMART	SM00505. Knot1. 1 hit. [Graphical view]
SUPFAM	SSF57095. SSF57095. 1 hit.
ProtoNet	Search...

Entry information

Entry name

SCX1_ANDAU

Accession

Primary (citable) accession number: P01479

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 1, 1993
Last modified:	May 1, 2013
This is version 88 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Animal Toxin Annotation Program