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Protein

Kappa-bungarotoxin

Gene
N/A
Organism
Bungarus multicinctus (Many-banded krait)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Postsynaptic neurotoxin that binds and inhibits neuronal nicotinic acetylcholine receptors (nAChR) with high affinity (IC50<100 nM). Is a selective, and slowly reversible antagonist of alpha-3/CHRNA3-containing and some alpha-4/CHRNA4-containing AChRs.1 Publication1 Publication

GO - Biological processi

Keywordsi

Molecular functionAcetylcholine receptor inhibiting toxin, Ion channel impairing toxin, Neurotoxin, Postsynaptic neurotoxin, Toxin

Names & Taxonomyi

Protein namesi
Recommended name:
Kappa-bungarotoxin2 Publications
Short name:
Kappa-bgt
Alternative name(s):
Kappa-1-bungarotoxin
Long neurotoxin 2
Neuronal bungarotoxin1 Publication
Short name:
nBgt1 Publication
Toxin F1 Publication
OrganismiBungarus multicinctus (Many-banded krait)
Taxonomic identifieri8616 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiLepidosauriaSquamataBifurcataUnidentataEpisquamataToxicoferaSerpentesColubroideaElapidaeBungarinaeBungarus

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi47Q → W: Little increase in inhibition of neuronal receptor and 8-fold increase in inhibition of muscle receptor. 1 Publication1
Mutagenesisi55R → A: Loss of inhibition of neuronal and muscle receptors. 1 Publication1
Mutagenesisi57P → A: No important change in inhibition of muscle and neuronal receptors. 1 Publication1
Mutagenesisi57P → K: 13-fold decrease in inhibition of neuronal receptor, no change in inhibition of muscle receptor. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 212 PublicationsAdd BLAST21
ChainiPRO_000003541022 – 87Kappa-bungarotoxinAdd BLAST66

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi24 ↔ 423 Publications
Disulfide bondi35 ↔ 633 Publications
Disulfide bondi48 ↔ 523 Publications
Disulfide bondi67 ↔ 793 Publications
Disulfide bondi80 ↔ 853 Publications

Keywords - PTMi

Disulfide bond

Expressioni

Tissue specificityi

Expressed by the venom gland.Curated

Interactioni

Subunit structurei

Homodimer (PubMed:7947721, PubMed:2036359) and heterodimer (By similarity); non-covalently linked.By similarity2 Publications

Structurei

Secondary structure

187
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi23 – 26Combined sources4
Turni27 – 30Combined sources4
Beta strandi31 – 34Combined sources4
Beta strandi37 – 39Combined sources3
Beta strandi41 – 47Combined sources7
Helixi52 – 55Combined sources4
Beta strandi58 – 66Combined sources9
Beta strandi74 – 82Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1KBAX-ray2.30A/B22-87[»]
2NBTNMR-A/B22-87[»]
ProteinModelPortaliP01398.
SMRiP01398.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP01398.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

HOVERGENiHBG006553.

Family and domain databases

CDDicd00206. snake_toxin. 1 hit.
InterProiView protein in InterPro
IPR003571. Snake_3FTx.
IPR018354. Snake_toxin_con_site.
PROSITEiView protein in PROSITE
PS00272. SNAKE_TOXIN. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P01398-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKTLLLTLVV VTIVCLDLGY TRTCLISPSS TPQTCPNGQD ICFLKAQCDK
60 70 80
FCSIRGPVIE QGCVATCPQF RSNYRSLLCC TTDNCNH
Length:87
Mass (Da):9,566
Last modified:November 1, 1997 - v2
Checksum:iE4950E1222066FB6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y08721 mRNA. Translation: CAA69971.1.
Y11768 Genomic DNA. Translation: CAA72433.1.
PIRiJC5383. N1KF2U.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y08721 mRNA. Translation: CAA69971.1.
Y11768 Genomic DNA. Translation: CAA72433.1.
PIRiJC5383. N1KF2U.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1KBAX-ray2.30A/B22-87[»]
2NBTNMR-A/B22-87[»]
ProteinModelPortaliP01398.
SMRiP01398.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

HOVERGENiHBG006553.

Miscellaneous databases

EvolutionaryTraceiP01398.

Family and domain databases

CDDicd00206. snake_toxin. 1 hit.
InterProiView protein in InterPro
IPR003571. Snake_3FTx.
IPR018354. Snake_toxin_con_site.
PROSITEiView protein in PROSITE
PS00272. SNAKE_TOXIN. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry namei3LKB_BUNMU
AccessioniPrimary (citable) accession number: P01398
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: November 1, 1997
Last modified: November 2, 2016
This is version 103 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
Annotation programAnimal Toxin Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.