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Protein

Alpha-elapitoxin-Nk2a

Gene
N/A
Organism
Naja kaouthia (Monocled cobra) (Naja siamensis)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The monomeric form binds with high affinity to muscular and neuronal alpha-7/CHRNA7 nicotinic acetylcholine receptors (nAChR). Causes paralysis by preventing acetylcholine binding to the nAChR. In mice lung cancer, causes reduction of tumor growth.1 Publication
The homodimeric form binds with low affinity to muscular and neuronal (alpha-7/CHRNA7) nAChRs, whereas it acquires the capacity to block alpha-3/beta-2 (CHRNA3/CHRNB2) nAChRs.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei23 – 231Selectively binds to Torpedo AChR
Sitei25 – 251Binds to both neuronal and Torpedo AChRs
Sitei26 – 261Selectively binds to alpha-7 AChR
Sitei27 – 271Binds to both neuronal and Torpedo AChRs
Sitei28 – 281Selectively binds to alpha-7 AChR
Sitei29 – 291Binds to both neuronal and Torpedo AChRs
Sitei30 – 301Selectively binds to alpha-7 AChR
Sitei33 – 331Binds to both neuronal and Torpedo AChRs
Sitei35 – 351Selectively binds to alpha-7 AChR
Sitei36 – 361Binds to both neuronal and Torpedo AChRs
Sitei49 – 491Selectively binds to Torpedo AChR
Sitei65 – 651Binds to both neuronal and Torpedo AChRs

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acetylcholine receptor inhibiting toxin, Ion channel impairing toxin, Neurotoxin, Postsynaptic neurotoxin, Toxin

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-elapitoxin-Nk2a
Short name:
Alpha-EPTX-Nk2a
Alternative name(s):
Alpha-cobratoxin
Short name:
Alpha-CbT
Short name:
Cbtx
Long neurotoxin 1
Neurotoxin 3
OrganismiNaja kaouthia (Monocled cobra) (Naja siamensis)
Taxonomic identifieri8649 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiLepidosauriaSquamataBifurcataUnidentataEpisquamataToxicoferaSerpentesColubroideaElapidaeElapinaeNaja

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Toxic dosei

LD50 is 0.1 mg/kg by intravenous injection into mice.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 7171Alpha-elapitoxin-Nk2aPRO_0000093554Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi3 ↔ 20
Disulfide bondi14 ↔ 41
Disulfide bondi26 ↔ 30
Disulfide bondi45 ↔ 56
Disulfide bondi57 ↔ 62

Keywords - PTMi

Disulfide bond

Expressioni

Tissue specificityi

Expressed by the venom gland.Curated

Interactioni

Subunit structurei

Monomer, homo- or heterodimer with cytotoxins 1, 2, and 3; disulfide-linked.7 Publications

Structurei

Secondary structure

1
71
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi6 – 105Combined sources
Beta strandi19 – 257Combined sources
Turni27 – 293Combined sources
Helixi30 – 334Combined sources
Beta strandi36 – 449Combined sources
Beta strandi52 – 576Combined sources
Beta strandi59 – 635Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1CTXX-ray2.80A1-71[»]
1LXGNMR-A1-71[»]
1LXHNMR-A1-71[»]
1YI5X-ray4.20F/G/H/I/J1-71[»]
4AEAX-ray1.94A/B1-71[»]
ProteinModelPortaliP01391.
SMRiP01391. Positions 1-71.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP01391.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

HOVERGENiHBG006553.

Family and domain databases

CDDicd00206. snake_toxin. 1 hit.
InterProiIPR003571. Snake_3FTx.
IPR018354. Snake_toxin_con_site.
[Graphical view]
PROSITEiPS00272. SNAKE_TOXIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P01391-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
IRCFITPDIT SKDCPNGHVC YTKTWCDAFC SIRGKRVDLG CAATCPTVKT
60 70
GVDIQCCSTD NCNPFPTRKR P
Length:71
Mass (Da):7,831
Last modified:July 21, 1986 - v1
Checksum:i6F07ADD885E9AC33
GO

Sequence databases

PIRiA01662. N2NJ1S.

Cross-referencesi

Sequence databases

PIRiA01662. N2NJ1S.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1CTXX-ray2.80A1-71[»]
1LXGNMR-A1-71[»]
1LXHNMR-A1-71[»]
1YI5X-ray4.20F/G/H/I/J1-71[»]
4AEAX-ray1.94A/B1-71[»]
ProteinModelPortaliP01391.
SMRiP01391. Positions 1-71.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

HOVERGENiHBG006553.

Miscellaneous databases

EvolutionaryTraceiP01391.

Family and domain databases

CDDicd00206. snake_toxin. 1 hit.
InterProiIPR003571. Snake_3FTx.
IPR018354. Snake_toxin_con_site.
[Graphical view]
PROSITEiPS00272. SNAKE_TOXIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry namei3L21_NAJKA
AccessioniPrimary (citable) accession number: P01391
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: September 7, 2016
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

The monomeric form has no effect on alpha-3/beta-2 (CHRNA3/CHRNB2) nAChR (PubMed:18381281). Does not show any blockade of the nicotine-evoked release of dopamine (PubMed:9840221) and does not affect ACh release.2 Publications

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.