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Protein

Alpha-elapitoxin-Nk2a

Gene
N/A
Organism
Naja kaouthia (Monocled cobra) (Naja siamensis)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The monomeric form binds with high affinity to muscular and neuronal alpha-7/CHRNA7 nicotinic acetylcholine receptors (nAChR). Causes paralysis by preventing acetylcholine binding to the nAChR. In mice lung cancer, causes reduction of tumor growth.1 Publication
The homodimeric form binds with low affinity to muscular and neuronal (alpha-7/CHRNA7) nAChRs, whereas it acquires the capacity to block alpha-3/beta-2 (CHRNA3/CHRNB2) nAChRs.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei23Selectively binds to Torpedo AChR1
Sitei25Binds to both neuronal and Torpedo AChRs1
Sitei26Selectively binds to alpha-7 AChR1
Sitei27Binds to both neuronal and Torpedo AChRs1
Sitei28Selectively binds to alpha-7 AChR1
Sitei29Binds to both neuronal and Torpedo AChRs1
Sitei30Selectively binds to alpha-7 AChR1
Sitei33Binds to both neuronal and Torpedo AChRs1
Sitei35Selectively binds to alpha-7 AChR1
Sitei36Binds to both neuronal and Torpedo AChRs1
Sitei49Selectively binds to Torpedo AChR1
Sitei65Binds to both neuronal and Torpedo AChRs1

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acetylcholine receptor inhibiting toxin, Ion channel impairing toxin, Neurotoxin, Postsynaptic neurotoxin, Toxin

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-elapitoxin-Nk2a
Short name:
Alpha-EPTX-Nk2a
Alternative name(s):
Alpha-cobratoxin
Short name:
Alpha-CbT
Short name:
Cbtx
Long neurotoxin 1
Neurotoxin 3
OrganismiNaja kaouthia (Monocled cobra) (Naja siamensis)
Taxonomic identifieri8649 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiLepidosauriaSquamataBifurcataUnidentataEpisquamataToxicoferaSerpentesColubroideaElapidaeElapinaeNaja

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Toxic dosei

LD50 is 0.1 mg/kg by intravenous injection into mice.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000935541 – 71Alpha-elapitoxin-Nk2aAdd BLAST71

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi3 ↔ 20
Disulfide bondi14 ↔ 41
Disulfide bondi26 ↔ 30
Disulfide bondi45 ↔ 56
Disulfide bondi57 ↔ 62

Keywords - PTMi

Disulfide bond

Expressioni

Tissue specificityi

Expressed by the venom gland.Curated

Interactioni

Subunit structurei

Monomer, homo- or heterodimer with cytotoxins 1, 2, and 3; disulfide-linked.7 Publications

Structurei

Secondary structure

171
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi6 – 10Combined sources5
Beta strandi19 – 25Combined sources7
Turni27 – 29Combined sources3
Helixi30 – 33Combined sources4
Beta strandi36 – 44Combined sources9
Beta strandi52 – 57Combined sources6
Beta strandi59 – 63Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1CTXX-ray2.80A1-71[»]
1LXGNMR-A1-71[»]
1LXHNMR-A1-71[»]
1YI5X-ray4.20F/G/H/I/J1-71[»]
4AEAX-ray1.94A/B1-71[»]
ProteinModelPortaliP01391.
SMRiP01391.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP01391.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

HOVERGENiHBG006553.

Family and domain databases

CDDicd00206. snake_toxin. 1 hit.
InterProiIPR003571. Snake_3FTx.
IPR018354. Snake_toxin_con_site.
[Graphical view]
PROSITEiPS00272. SNAKE_TOXIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P01391-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
IRCFITPDIT SKDCPNGHVC YTKTWCDAFC SIRGKRVDLG CAATCPTVKT
60 70
GVDIQCCSTD NCNPFPTRKR P
Length:71
Mass (Da):7,831
Last modified:July 21, 1986 - v1
Checksum:i6F07ADD885E9AC33
GO

Sequence databases

PIRiA01662. N2NJ1S.

Cross-referencesi

Sequence databases

PIRiA01662. N2NJ1S.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1CTXX-ray2.80A1-71[»]
1LXGNMR-A1-71[»]
1LXHNMR-A1-71[»]
1YI5X-ray4.20F/G/H/I/J1-71[»]
4AEAX-ray1.94A/B1-71[»]
ProteinModelPortaliP01391.
SMRiP01391.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

HOVERGENiHBG006553.

Miscellaneous databases

EvolutionaryTraceiP01391.

Family and domain databases

CDDicd00206. snake_toxin. 1 hit.
InterProiIPR003571. Snake_3FTx.
IPR018354. Snake_toxin_con_site.
[Graphical view]
PROSITEiPS00272. SNAKE_TOXIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry namei3L21_NAJKA
AccessioniPrimary (citable) accession number: P01391
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: November 2, 2016
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

The monomeric form has no effect on alpha-3/beta-2 (CHRNA3/CHRNB2) nAChR (PubMed:18381281). Does not show any blockade of the nicotine-evoked release of dopamine (PubMed:9840221) and does not affect ACh release.2 Publications

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.