Reviewed, UniProtKB/Swiss-Prot P01356 (CCKN_PIG)

Names and origin

Protein names

Recommended name:
    Cholecystokinin
      Short name=CCK
Cleaved into the following 10 chains:
    1- Recommended name:
            Cholecystokinin-58
                Short name=CCK58
    2- Recommended name:
            Cholecystokinin-58 desnonopeptide
        Alternative name(s):
            (1-49)-CCK58
    3- Recommended name:
            Cholecystokinin-39
                Short name=CCK39
    4- Recommended name:
            Cholecystokinin-33
                Short name=CCK33
    5- Recommended name:
            Cholecystokinin-25
                Short name=CCK25
    6- Recommended name:
            Cholecystokinin-18
                Short name=CCK18
    7- Recommended name:
            Cholecystokinin-12
                Short name=CCK12
    8- Recommended name:
            Cholecystokinin-8
                Short name=CCK8
    9- Recommended name:
            Cholecystokinin-7
                Short name=CCK7
    10- Recommended name:
            Cholecystokinin-5
                Short name=CCK5

Gene names

Name:

CCK

Organism

Sus scrofa (Pig)

Taxonomic identifier

9823 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Suina › Suidae › Sus

Protein attributes

Sequence length	114 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	This peptide hormone induces gall bladder contraction and the release of pancreatic enzymes in the gut. Its function in the brain is not clear. Binding to CCK-A receptors stimulates amylase release from the pancreas, binding to CCK-B receptors stimulates gastric acid secretion.
Subunit structure	Binds to CCK-A receptors in the pancreas and CCK-B receptors in the brain By similarity.
Subcellular location	Secreted.
Tissue specificity	Synthesized in both cerebral cortex and duodenal mucosa.
Post-translational modification	The precursor is cleaved by proteases to produce a number of active cholecystokinins. Brain contains CCK-octapeptide (CCK8) and several CCK-desoctapeptides; whereas pig gut contains intact CCK33, CCK39, and CCK58 as well as CCK-octapeptide and the CCK-desoctapeptides. Distribution differences are due to tissue-specific post-translational processing events.
Sequence similarities	Belongs to the gastrin/cholecystokinin family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Hormone
PTM	Amidation Cleavage on pair of basic residues Sulfation
Technical term	Direct protein sequencing
Gene Ontology (GO)
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	hormone activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	Probable
	Chain	21 – 114	94	Cholecystokinin		PRO_0000010558
	Peptide	45 – 102	58	Cholecystokinin-58		PRO_0000010559
	Peptide	46 – 94	49	Cholecystokinin-58 desnonopeptide By similarity		PRO_0000306314
	Peptide	64 – 102	39	Cholecystokinin-39		PRO_0000010560
	Peptide	70 – 102	33	Cholecystokinin-33		PRO_0000010561
	Peptide	79 – 103	25	Cholecystokinin-25 By similarity		PRO_0000306315
	Peptide	86 – 103	18	Cholecystokinin-18 By similarity		PRO_0000306316
	Peptide	91 – 102	12	Cholecystokinin-12		PRO_0000010562
	Peptide	95 – 102	8	Cholecystokinin-8		PRO_0000010563
	Peptide	97 – 103	7	Cholecystokinin-7 By similarity		PRO_0000306317
	Peptide	99 – 103	5	Cholecystokinin-5 By similarity		PRO_0000306318
	Propeptide	106 – 114	9			PRO_0000010564
Amino acid modifications
	Modified residue	96	1	Sulfotyrosine Ref.3
	Modified residue	102	1	Phenylalanine amide Ref.3
	Modified residue	110	1	Sulfotyrosine
	Modified residue	112	1	Sulfotyrosine

Sequences

	Sequence		Length	Mass (Da)
	P01356-1 [UniParc]. Last modified October 23, 1986. Version 1. Checksum: 8F8C05DF29B0E6EE	FASTA	114	12,526
	10 20 30 40 50 60 MNGGLCLCVL MAVLAAGTLA QPVPPADSAV PGAQEEEAHR RQLRAVQKVD GESRAHLGAL 70 80 90 100 110 LARYIQQARK APSGRVSMIK NLQSLDPSHR ISDRDYMGWM DFGRRSAEEY EYTS

References

[1]	"Cloned cDNA to cholecystokinin mRNA predicts an identical preprocholecystokinin in pig brain and gut." Gubler U., Chua A.O., Hoffman B.J., Collier K.J., Eng J. Proc. Natl. Acad. Sci. U.S.A. 81:4307-4310(1984) [PubMed: 6205394] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	Mutt V., Jorpes J.E. Submitted (AUG-1970) to the PIR data bank Cited for: PROTEIN SEQUENCE OF 70-102.
[3]	"Synthesis of cholecystokinin-pancreozymin. I. The C-terminal dodecapeptide." Ondetti M.A., Pluscec J., Sabo E.F., Sheehan J.T., Williams N. J. Am. Chem. Soc. 92:195-199(1970) [PubMed: 5410106] [Abstract] Cited for: PROTEIN SEQUENCE OF 91-102.
[4]	"Analysis of sequence requirements for protein tyrosine sulfation." Rosenquist G.L., Nicholas H.B. Protein Sci. 2:215-222(1993) [PubMed: 8443599] [Abstract] Cited for: SULFATION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	K01940 mRNA. Translation: AAA31014.1.
PIR	GMPGCP. A05084.
RefSeq	NP_999402.1.
UniGene	Ssc.717
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	397468.
KEGG	ssc:397468.
Phylogenomic databases
HOVERGEN	P01356.
Family and domain databases
InterPro	IPR015499. CCK. IPR001651. Gastrin. IPR013152. Gastrin/cholecystokinin_CS. [Graphical view]
PANTHER	PTHR10786. CCK. 1 hit.
Pfam	PF00918. Gastrin. 1 hit. [Graphical view]
SMART	SM00029. GASTRIN. 1 hit. [Graphical view]
PROSITE	PS00259. GASTRIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CCKN_PIG

Accession

Primary (citable) accession number: P01356

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	October 23, 1986
Last modified:	June 16, 2009
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)