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Protein

Cholecystokinin

Gene

Cck

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

This peptide hormone induces gall bladder contraction and the release of pancreatic enzymes in the gut. Its function in the brain is not clear. Binding to CCK-A receptors stimulates amylase release from the pancreas, binding to CCK-B receptors stimulates gastric acid secretion.

GO - Molecular functioni

  • hormone activity Source: RGD
  • neuropeptide hormone activity Source: UniProtKB

GO - Biological processi

  • activation of cysteine-type endopeptidase activity involved in apoptotic process Source: RGD
  • apoptotic process Source: RGD
  • axonogenesis Source: UniProtKB
  • behavioral fear response Source: RGD
  • eating behavior Source: UniProtKB
  • negative regulation of appetite Source: RGD
  • neuron migration Source: UniProtKB
  • positive regulation of apoptotic process Source: RGD
  • positive regulation of cell proliferation Source: RGD
  • positive regulation of glutamate secretion Source: CACAO
  • positive regulation of mitochondrial depolarization Source: RGD
  • positive regulation of peptidyl-tyrosine phosphorylation Source: RGD
  • positive regulation of protein oligomerization Source: RGD
  • protein kinase C-activating G-protein coupled receptor signaling pathway Source: RGD
  • regulation of sensory perception of pain Source: RGD
  • release of cytochrome c from mitochondria Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Hormone

Enzyme and pathway databases

ReactomeiR-RNO-375276. Peptide ligand-binding receptors.
R-RNO-416476. G alpha (q) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
Cholecystokinin
Short name:
CCK
Cleaved into the following 5 chains:
Cholecystokinin-39
Short name:
CCK39
Cholecystokinin-33
Short name:
CCK33
Cholecystokinin-22
Short name:
CCK22
Cholecystokinin-12
Short name:
CCK12
Cholecystokinin-8
Short name:
CCK8
Gene namesi
Name:Cck
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 8

Organism-specific databases

RGDi2288. Cck.

Subcellular locationi

GO - Cellular componenti

  • axon Source: UniProtKB
  • axon hillock Source: RGD
  • axon initial segment Source: RGD
  • dendrite Source: RGD
  • extracellular region Source: RGD
  • extracellular space Source: RGD
  • neuronal cell body Source: RGD
  • perikaryon Source: RGD
  • terminal bouton Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020By similarityAdd
BLAST
Propeptidei21 – 4525PRO_0000010565Add
BLAST
Chaini46 – 10358CholecystokininPRO_0000010566Add
BLAST
Propeptidei47 – 6317PRO_0000010567Add
BLAST
Peptidei65 – 10339Cholecystokinin-39PRO_0000010568Add
BLAST
Peptidei71 – 10333Cholecystokinin-33PRO_0000010569Add
BLAST
Peptidei82 – 10322Cholecystokinin-22PRO_0000010570Add
BLAST
Peptidei92 – 10312Cholecystokinin-12PRO_0000010571Add
BLAST
Peptidei96 – 1038Cholecystokinin-8PRO_0000010572
Propeptidei107 – 1159PRO_0000010573

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei97 – 971Sulfotyrosine1 Publication
Modified residuei103 – 1031Phenylalanine amide
Modified residuei111 – 1111Sulfotyrosine1 Publication
Modified residuei113 – 1131Sulfotyrosine1 Publication

Post-translational modificationi

The precursor is cleaved by proteases to produce a number of active cholecystokinins.
Sulfation of Tyr-97 is essential for receptor activation.2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei106 – 1072Cleavage

Keywords - PTMi

Amidation, Cleavage on pair of basic residues, Sulfation

Proteomic databases

PaxDbiP01355.
PRIDEiP01355.

Miscellaneous databases

PMAP-CutDBP01355.

Expressioni

Tissue specificityi

The shortest form (CCK8) is predominantly found in the brain, whereas the larger ones are found in the intestine.

Gene expression databases

GenevisibleiP01355. RN.

Interactioni

Subunit structurei

Binds to CCK-A receptors in the pancreas and CCK-B receptors in the brain.By similarity

GO - Molecular functioni

  • hormone activity Source: RGD
  • neuropeptide hormone activity Source: UniProtKB

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000026159.

Chemistry

BindingDBiP01355.

Structurei

3D structure databases

ProteinModelPortaliP01355.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the gastrin/cholecystokinin family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IXQ9. Eukaryota.
ENOG411211F. LUCA.
GeneTreeiENSGT00390000003571.
HOGENOMiHOG000073533.
HOVERGENiHBG005806.
InParanoidiP01355.
KOiK05226.
OMAiLARYMHQ.
OrthoDBiEOG7F7WBS.
PhylomeDBiP01355.
TreeFamiTF333419.

Family and domain databases

InterProiIPR015499. CCK-like.
IPR001651. Gastrin.
IPR013152. Gastrin/cholecystokinin_CS.
[Graphical view]
PANTHERiPTHR10786. PTHR10786. 1 hit.
PfamiPF00918. Gastrin. 1 hit.
[Graphical view]
PROSITEiPS00259. GASTRIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P01355-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKCGVCLCVV MAVLAAGALA QPVVPVEAVD PMEQRAEEAP RRQLRAVLRP
60 70 80 90 100
DSEPRARLGA LLARYIQQVR KAPSGRMSVL KNLQGLDPSH RISDRDYMGW
110
MDFGRRSAED YEYPS
Length:115
Mass (Da):12,841
Last modified:July 21, 1986 - v1
Checksum:i0A713472CAD0F3DF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X01032 mRNA. Translation: CAA25517.1.
K01259 Genomic DNA. Translation: AAA40897.1.
M25942 mRNA. Translation: AAA40898.1.
M10353, M10352 Genomic DNA. Translation: AAB61086.1.
PIRiA01624. GMRTCP.
RefSeqiNP_036961.1. NM_012829.2.
UniGeneiRn.9781.

Genome annotation databases

EnsembliENSRNOT00000026159; ENSRNOP00000026159; ENSRNOG00000019321.
GeneIDi25298.
KEGGirno:25298.
UCSCiRGD:2288. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X01032 mRNA. Translation: CAA25517.1.
K01259 Genomic DNA. Translation: AAA40897.1.
M25942 mRNA. Translation: AAA40898.1.
M10353, M10352 Genomic DNA. Translation: AAB61086.1.
PIRiA01624. GMRTCP.
RefSeqiNP_036961.1. NM_012829.2.
UniGeneiRn.9781.

3D structure databases

ProteinModelPortaliP01355.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000026159.

Chemistry

BindingDBiP01355.

Proteomic databases

PaxDbiP01355.
PRIDEiP01355.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000026159; ENSRNOP00000026159; ENSRNOG00000019321.
GeneIDi25298.
KEGGirno:25298.
UCSCiRGD:2288. rat.

Organism-specific databases

CTDi885.
RGDi2288. Cck.

Phylogenomic databases

eggNOGiENOG410IXQ9. Eukaryota.
ENOG411211F. LUCA.
GeneTreeiENSGT00390000003571.
HOGENOMiHOG000073533.
HOVERGENiHBG005806.
InParanoidiP01355.
KOiK05226.
OMAiLARYMHQ.
OrthoDBiEOG7F7WBS.
PhylomeDBiP01355.
TreeFamiTF333419.

Enzyme and pathway databases

ReactomeiR-RNO-375276. Peptide ligand-binding receptors.
R-RNO-416476. G alpha (q) signalling events.

Miscellaneous databases

NextBioi606067.
PMAP-CutDBP01355.
PROiP01355.

Gene expression databases

GenevisibleiP01355. RN.

Family and domain databases

InterProiIPR015499. CCK-like.
IPR001651. Gastrin.
IPR013152. Gastrin/cholecystokinin_CS.
[Graphical view]
PANTHERiPTHR10786. PTHR10786. 1 hit.
PfamiPF00918. Gastrin. 1 hit.
[Graphical view]
PROSITEiPS00259. GASTRIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Molecular cloning and nucleotide sequence of cDNA coding for rat brain cholecystokinin precursor."
    Kuwano R., Araki K., Usui H., Fukui T., Ohtsuka E., Ikehara M., Takahashi Y.
    J. Biochem. 96:923-926(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Cloning and sequence analysis of a cDNA encoding rat preprocholecystokinin."
    Deschenes R.J., Lorenz L.J., Haun R.S., Roos B.A., Collier K.J., Dixon J.E.
    Proc. Natl. Acad. Sci. U.S.A. 81:726-730(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  4. "A gene encoding rat cholecystokinin. Isolation, nucleotide sequence, and promoter activity."
    Deschenes R.J., Haun R.S., Funckes C.L., Dixon J.E.
    J. Biol. Chem. 260:1280-1286(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  5. "Isolation, structure and properties of the C-terminal fragment of the rat cholecystokinin precursor."
    Varro A., Young J., Gregory H., Csech J., Dockray G.J.
    Regul. Pept. 15:195-195(1986)
    Cited for: SULFATION AT TYR-111 AND TYR-113.
  6. "Inhibition of pro-cholecystokinin (CCK) sulfation by treatment with sodium chlorate alters its processing and decreases cellular content and secretion of CCK 8."
    Beinfeld M.C.
    Neuropeptides 26:195-200(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: SULFATION AT TYR-97.
  7. "Biosynthesis and processing of pro CCK: recent progress and future challenges."
    Beinfeld M.C.
    Life Sci. 72:747-757(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.

Entry informationi

Entry nameiCCKN_RAT
AccessioniPrimary (citable) accession number: P01355
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: May 11, 2016
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.