P01353 (GAST_CANFA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Gastrin

Cleaved into the following 2 chains:

1. Big gastrin
   Alternative name(s):
   Gastrin-34
   Short name=G34
2. Gastrin

Gene names

Name:	GAST
Synonyms:	GAS

Organism

Canis familiaris (Dog) (Canis lupus familiaris)

Taxonomic identifier

9615 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Carnivora › Caniformia › Canidae › Canis

Protein attributes

Sequence length	104 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Gastrin stimulates the stomach mucosa to produce and secrete hydrochloric acid and the pancreas to secrete its digestive enzymes. It also stimulates smooth muscle contraction and increases blood circulation and water secretion in the stomach and intestine.
Subcellular location	Secreted.
Sequence similarities	Belongs to the gastrin/cholecystokinin family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Hormone
PTM	Amidation Cleavage on pair of basic residues Phosphoprotein Pyrrolidone carboxylic acid Sulfation
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	hormone activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	Potential
	Propeptide	22 – 58	37			PRO_0000010613
	Peptide	59 – 92	34	Big gastrin Ref.2		PRO_0000010614
	Peptide	76 – 92	17	Gastrin Ref.3		PRO_0000010615
	Propeptide	96 – 104	9			PRO_0000010616
Amino acid modifications
	Modified residue	59	1	Pyrrolidone carboxylic acid Ref.2
	Modified residue	76	1	Pyrrolidone carboxylic acid Ref.2
	Modified residue	87	1	Sulfotyrosine
	Modified residue	92	1	Phenylalanine amide Ref.2
	Modified residue	96	1	Phosphoserine
Natural variations
	Natural variant	85	1	A → T.
Experimental info
	Sequence conflict	83 – 85	3	EEA → AEE AA sequence Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P01353 [UniParc]. Last modified July 15, 1998. Version 3. Checksum: 73BF72A18DFE78CA	FASTA	104	11,519
	10 20 30 40 50 60 MQRLCVYVLI LALALATFSE ASWKPRSRLQ DAPSGPGANR GLEPHGLDQL GPASHHRRQL 70 80 90 100 GLQGPPQLVA DLSKKQGPWM EEEEAAYGWM DFGRRSAEEG DQRP

References

[1]	"Cloning of canine gastrin cDNA's encoding variant amino acid sequences." Gantz I., Takeuchi T., Yamada T. Digestion 46:99-104(1990) [PubMed: 2262079] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Gastric mucosa.
[2]	"Sequences of gastrins purified from a single antrum of dog and of goat." Bonato C., Eng J., Hulmes J.D., Miedel M., Pan Y.-C.E., Yalow R.S. Peptides 7:689-693(1986) [PubMed: 3763441] [Abstract] Cited for: PROTEIN SEQUENCE OF 59-92. Tissue: Gastric mucosa.
[3]	"Structure and synthesis of canine gastrin." Agarwal K.L., Kenner G.W., Sheppard R.C. Experientia 25:346-348(1969) [PubMed: 5799207] [Abstract] Cited for: PROTEIN SEQUENCE OF 76-92, PHOSPHORYLATION.
[4]	"The constitution and properties of phosphorylated and unphosphorylated C-terminal fragments of progastrin from dog and ferret antrum." Desmond H., Varro A., Young J., Gregory H., Nemeth J., Dockray G.J. Regul. Pept. 25:223-233(1989) [PubMed: 2756156] [Abstract] Cited for: PROTEIN SEQUENCE OF 96-104. Tissue: Gastric mucosa.

Cross-references

Sequence databases
PIR	GMDG. B61053.
RefSeq	XP_003435286.1. XM_003435238.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	P01353.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSCAFT00000025228; ENSCAFP00000023420; ENSCAFG00000015924.
GeneID	100685087.
Phylogenomic databases
eggNOG	maNOG20875.
GeneTree	ENSGT00390000014792.
HOVERGEN	HBG097593.
InParanoid	P01353.
OMA	KPRSQLQ.
OrthoDB	EOG4CG09S.
PhylomeDB	P01353.
Family and domain databases
InterPro	IPR001651. Gastrin. IPR013152. Gastrin/cholecystokinin_CS. [Graphical view]
Pfam	PF00918. Gastrin. 1 hit. [Graphical view]
SMART	SM00029. GASTRIN. 1 hit. [Graphical view]
PROSITE	PS00259. GASTRIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GAST_CANFA

Accession

Primary (citable) accession number: P01353

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 15, 1998
Last modified:	November 16, 2011
This is version 84 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program