P01352 (GAST_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Gastrin

Cleaved into the following 2 chains:

1. Big gastrin
   Alternative name(s):
   Gastrin-34
   Short name=G34
2. Gastrin

Gene names

Name:	GAST
Synonyms:	GAS

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	104 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Gastrin stimulates the stomach mucosa to produce and secrete hydrochloric acid and the pancreas to secrete its digestive enzymes. It also stimulates smooth muscle contraction and increases blood circulation and water secretion in the stomach and intestine.
Subcellular location	Secreted.
Sequence similarities	Belongs to the gastrin/cholecystokinin family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Hormone
PTM	Amidation Cleavage on pair of basic residues Phosphoprotein Pyrrolidone carboxylic acid Sulfation
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	hormone activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21
	Propeptide	22 – 58	37			PRO_0000010609
	Peptide	59 – 92	34	Big gastrin		PRO_0000010610
	Peptide	76 – 92	17	Gastrin Ref.2 Ref.3		PRO_0000010611
	Propeptide	96 – 104	9			PRO_0000010612
Amino acid modifications
	Modified residue	59	1	Pyrrolidone carboxylic acid
	Modified residue	76	1	Pyrrolidone carboxylic acid Ref.3
	Modified residue	87	1	Sulfotyrosine Ref.3
	Modified residue	92	1	Phenylalanine amide Ref.3
	Modified residue	96	1	Phosphoserine By similarity
Experimental info
	Sequence conflict	32	1	A → L in AAA30537. Ref.1
	Sequence conflict	37	1	G → R in AAA30537. Ref.1
	Sequence conflict	48	1	N → T in AAA30537. Ref.1
	Sequence conflict	74	1	K → N in AAA30537. Ref.1
	Sequence conflict	81	1	E → G in AAA30537. Ref.1
	Sequence conflict	96	1	S → M in AAA30537. Ref.1
	Sequence conflict	99	1	E → G in AAA30537. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P01352 [UniParc]. Last modified November 1, 1991. Version 2. Checksum: 54D03BF200F299F2	FASTA	104	11,573
	10 20 30 40 50 60 MQRLCAHVLI LVLALAAFCE ASWKPHSHLQ DAPVAPGANR GQEPLRMNRL GPASNPRRQL 70 80 90 100 GLQDPPHMVA DLSKKQGPWV EEEEAAYGWM DFGRRSAEEG DQHP

References

[1]	"Cloning and sequencing of the bovine gastrin gene." Lund T., Olsen J., Rehfeld J.F. Mol. Endocrinol. 3:1585-1588(1989) [PubMed: 2608050] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Tissue: Liver.
[2]	"Bovine and feline gastrin cDNA sequences and the amino acid and nucleotide sequence homologies among mammalian species." Kim S.J., Uhm K.N., Kang Y.K., Yoo O.J. DNA Seq. 1:181-187(1991) [PubMed: 1773057] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
[3]	"Isolation, structure and synthesis of ovine and bovine gastrins." Agarwal K.L., Beacham J., Bentley P.H., Gregory R.A., Kenner G.W., Sheppard R.C., Tracy H.J. Nature 219:614-615(1968) [PubMed: 5665711] [Abstract] Cited for: PROTEIN SEQUENCE OF 76-92.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M31657 Genomic DNA. Translation: AAA30537.1. X16581 mRNA. Translation: CAA34598.1.
IPI	IPI00694911.
PIR	GMBO. S14400.
RefSeq	NP_776340.1. NM_173915.2.
UniGene	Bt.312.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	P01352.
Proteomic databases
PRIDE	P01352.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSBTAT00000023489; ENSBTAP00000023489; ENSBTAG00000017663.
GeneID	280800.
KEGG	bta:280800.
Organism-specific databases
CTD	2520.
Phylogenomic databases
eggNOG	maNOG20875.
GeneTree	ENSGT00390000014792.
HOVERGEN	HBG097593.
InParanoid	P01352.
OrthoDB	EOG4CG09S.
Family and domain databases
InterPro	IPR001651. Gastrin. IPR013152. Gastrin/cholecystokinin_CS. [Graphical view]
KO	K13768.
Pfam	PF00918. Gastrin. 1 hit. [Graphical view]
SMART	SM00029. GASTRIN. 1 hit. [Graphical view]
PROSITE	PS00259. GASTRIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GAST_BOVIN

Accession

Primary (citable) accession number: P01352
Secondary accession number(s): Q28114

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	November 1, 1991
Last modified:	November 16, 2011
This is version 87 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program