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Protein

Insulin-like growth factor II

Gene

Igf2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The insulin-like growth factors possess growth-promoting activity. In vitro, they are potent mitogens for cultured cells. IGF-II is influenced by placental lactogen and may play a role in fetal development.
Preptin undergoes glucose-mediated co-secretion with insulin, and acts as physiological amplifier of glucose-mediated insulin secretion. Exhibits osteogenic properties by increasing osteoblast mitogenic activity through phosphoactivation of MAPK1 and MAPK3.

GO - Molecular functioni

GO - Biological processi

  • cellular response to mechanical stimulus Source: RGD
  • female pregnancy Source: RGD
  • glucose metabolic process Source: UniProtKB-KW
  • memory Source: RGD
  • negative regulation of natural killer cell mediated cytotoxicity Source: RGD
  • ossification Source: UniProtKB-KW
  • positive regulation of cell division Source: UniProtKB-KW
  • positive regulation of steroid hormone biosynthetic process Source: BHF-UCL
  • response to drug Source: RGD
  • response to estradiol Source: RGD
  • response to ethanol Source: RGD
  • response to nicotine Source: RGD
  • response to nutrient levels Source: RGD
  • response to organic cyclic compound Source: RGD
  • response to radiation Source: RGD
  • wound healing Source: RGD

Keywordsi

Molecular functionGrowth factor, Hormone, Mitogen
Biological processCarbohydrate metabolism, Glucose metabolism, Osteogenesis

Enzyme and pathway databases

ReactomeiR-RNO-114608. Platelet degranulation.
R-RNO-2404192. Signaling by Type 1 Insulin-like Growth Factor 1 Receptor (IGF1R).
R-RNO-2428928. IRS-related events triggered by IGF1R.
R-RNO-2428933. SHC-related events triggered by IGF1R.
R-RNO-381426. Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs).

Names & Taxonomyi

Protein namesi
Recommended name:
Insulin-like growth factor II
Short name:
IGF-II
Alternative name(s):
Multiplication-stimulating activity
Short name:
MSA
Multiplication-stimulating polypeptide
Cleaved into the following 2 chains:
Gene namesi
Name:Igf2
Synonyms:Igf-2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi2870. Igf2.

Subcellular locationi

  • Secreted By similarity

GO - Cellular componenti

  • extracellular space Source: RGD

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 241 PublicationAdd BLAST24
ChainiPRO_000001572925 – 91Insulin-like growth factor IIAdd BLAST67
PropeptideiPRO_000001573092 – 180E peptideAdd BLAST89
PeptideiPRO_000037038093 – 126PreptinAdd BLAST34

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi33 ↔ 71By similarity
Disulfide bondi45 ↔ 84By similarity
Disulfide bondi70 ↔ 75By similarity

Post-translational modificationi

Proteolytically processed by PCSK4, proIGF2 is cleaved at Arg-128 and Arg-92 to generate big-IGF2 and mature IGF2.By similarity

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond

Proteomic databases

PaxDbiP01346.
PRIDEiP01346.

Expressioni

Gene expression databases

BgeeiENSRNOG00000020369.
ExpressionAtlasiP01346. baseline.
GenevisibleiP01346. RN.

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

IntActiP01346. 1 interactor.
STRINGi10116.ENSRNOP00000047037.

Structurei

3D structure databases

ProteinModelPortaliP01346.
SMRiP01346.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni25 – 52BAdd BLAST28
Regioni53 – 64CAdd BLAST12
Regioni65 – 85AAdd BLAST21
Regioni86 – 91D6

Sequence similaritiesi

Belongs to the insulin family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IY3P. Eukaryota.
ENOG4111KP2. LUCA.
GeneTreeiENSGT00530000063856.
HOGENOMiHOG000233362.
HOVERGENiHBG006137.
InParanoidiP01346.
KOiK13769.
PhylomeDBiP01346.
TreeFamiTF332820.

Family and domain databases

Gene3Di1.10.100.10. 1 hit.
InterProiView protein in InterPro
IPR022334. IGF2.
IPR013576. IGF2_C.
IPR016179. Insulin-like.
IPR022350. Insulin-like_growth_factor.
IPR022353. Insulin_CS.
IPR022352. Insulin_family.
PfamiView protein in Pfam
PF08365. IGF2_C. 1 hit.
PF00049. Insulin. 1 hit.
PRINTSiPR02002. INSLNLIKEGF.
PR02006. INSLNLIKEGF2.
PR00276. INSULINFAMLY.
ProDomiView protein in ProDom or Entries sharing at least one domain
PD005188. IGF2_C. 1 hit.
SMARTiView protein in SMART
SM00078. IlGF. 1 hit.
SUPFAMiSSF56994. SSF56994. 1 hit.
PROSITEiView protein in PROSITE
PS00262. INSULIN. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P01346-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGIPVGKSML VLLISLAFAL CCIAAYRPSE TLCGGELVDT LQFVCSDRGF
60 70 80 90 100
YFSRPSSRAN RRSRGIVEEC CFRSCDLALL ETYCATPAKS ERDVSTSQAV
110 120 130 140 150
LPDDFPRYPV GKFFKFDTWR QSAGRLRRGL PALLRARRGR MLAKELEAFR
160 170 180
EAKRHRPLIV LPPKDPAHGG ASSEMSSNHQ
Length:180
Mass (Da):20,086
Last modified:March 20, 1987 - v1
Checksum:iAF12B4EEC0DBCC34
GO

Sequence cautioni

The sequence CAA25427 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence CAA25429 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti1 – 8Missing (PubMed:3889836).Curated8
Sequence conflicti57S → G (PubMed:2438416).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X00911 mRNA. Translation: CAA25428.1.
X00911 mRNA. Translation: CAA25427.1. Different initiation.
X00911 mRNA. Translation: CAA25429.1. Different initiation.
X02213 mRNA. Translation: CAA26136.1.
X13101 mRNA. Translation: CAA31493.1.
X14833 mRNA. Translation: CAA32942.1.
X14834 mRNA. Translation: CAA32943.1.
M13871, M13869, M13870 Genomic DNA. Translation: AAB95624.1.
M29880, M29879 Genomic DNA. Translation: AAA41391.1.
M30273 mRNA. Translation: AAA41432.1.
M31221 Genomic DNA. Translation: AAA42046.1.
PIRiA25350. IGRT2.
RefSeqiNP_001177091.1. NM_001190162.1.
NP_001177092.1. NM_001190163.1.
NP_113699.2. NM_031511.2.
XP_006230727.1. XM_006230665.2.
UniGeneiRn.118681.
Rn.224460.

Genome annotation databases

EnsembliENSRNOT00000050760; ENSRNOP00000047037; ENSRNOG00000020369.
ENSRNOT00000073850; ENSRNOP00000066983; ENSRNOG00000020369.
ENSRNOT00000089171; ENSRNOP00000070557; ENSRNOG00000020369.
GeneIDi24483.
KEGGirno:24483.
UCSCiRGD:2870. rat.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiIGF2_RAT
AccessioniPrimary (citable) accession number: P01346
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: March 20, 1987
Last modified: May 10, 2017
This is version 148 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families