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P01346

- IGF2_RAT

UniProt

P01346 - IGF2_RAT

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Protein
Insulin-like growth factor II
Gene
Igf2, Igf-2
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

The insulin-like growth factors possess growth-promoting activity. In vitro, they are potent mitogens for cultured cells. IGF-II is influenced by placental lactogen and may play a role in fetal development.1 Publication
Preptin undergoes glucose-mediated co-secretion with insulin, and acts as physiological amplifier of glucose-mediated insulin secretion. Exhibits osteogenic properties by increasing osteoblast mitogenic activity through phosphoactivation of MAPK1 and MAPK3.1 Publication

GO - Molecular functioni

  1. growth factor activity Source: RGD
  2. protein binding Source: UniProtKB
  3. protein serine/threonine kinase activator activity Source: Ensembl
  4. receptor activator activity Source: Ensembl

GO - Biological processi

  1. cell proliferation Source: RGD
  2. cellular response to mechanical stimulus Source: RGD
  3. exocrine pancreas development Source: Ensembl
  4. female pregnancy Source: RGD
  5. glucose metabolic process Source: UniProtKB-KW
  6. insulin receptor signaling pathway via phosphatidylinositol 3-kinase Source: Ensembl
  7. memory Source: RGD
  8. organ morphogenesis Source: Ensembl
  9. osteoblast differentiation Source: Ensembl
  10. positive regulation of MAPK cascade Source: Ensembl
  11. positive regulation of activated T cell proliferation Source: Ensembl
  12. positive regulation of cell division Source: UniProtKB-KW
  13. positive regulation of glycogen (starch) synthase activity Source: Ensembl
  14. positive regulation of glycogen biosynthetic process Source: Ensembl
  15. positive regulation of insulin receptor signaling pathway Source: Ensembl
  16. positive regulation of mitosis Source: Ensembl
  17. positive regulation of peptidyl-tyrosine phosphorylation Source: Ensembl
  18. positive regulation of protein kinase B signaling Source: Ensembl
  19. positive regulation of steroid hormone biosynthetic process Source: BHF-UCL
  20. positive regulation of transcription from RNA polymerase II promoter Source: Ensembl
  21. response to drug Source: RGD
  22. response to estradiol Source: RGD
  23. response to ethanol Source: RGD
  24. response to nicotine Source: RGD
  25. response to nutrient levels Source: RGD
  26. response to organic cyclic compound Source: RGD
  27. response to radiation Source: RGD
  28. striated muscle cell differentiation Source: Ensembl
  29. wound healing Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Growth factor, Hormone, Mitogen

Keywords - Biological processi

Carbohydrate metabolism, Glucose metabolism, Osteogenesis

Enzyme and pathway databases

ReactomeiREACT_194916. SHC-related events triggered by IGF1R.
REACT_208985. Signaling by Type 1 Insulin-like Growth Factor 1 Receptor (IGF1R).
REACT_222757. IRS-related events triggered by IGF1R.

Names & Taxonomyi

Protein namesi
Recommended name:
Insulin-like growth factor II
Short name:
IGF-II
Alternative name(s):
Multiplication-stimulating activity
Short name:
MSA
Multiplication-stimulating polypeptide
Cleaved into the following 2 chains:
Gene namesi
Name:Igf2
Synonyms:Igf-2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 1

Organism-specific databases

RGDi2870. Igf2.

Subcellular locationi

GO - Cellular componenti

  1. extracellular space Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 24241 Publication
Add
BLAST
Chaini25 – 9167Insulin-like growth factor II
PRO_0000015729Add
BLAST
Propeptidei92 – 18089E peptide
PRO_0000015730Add
BLAST
Peptidei93 – 12634Preptin
PRO_0000370380Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi33 ↔ 71 By similarity
Disulfide bondi45 ↔ 84 By similarity
Disulfide bondi70 ↔ 75 By similarity

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond

Proteomic databases

PaxDbiP01346.
PRIDEiP01346.

Expressioni

Gene expression databases

ArrayExpressiP01346.
GenevestigatoriP01346.

Interactioni

Protein-protein interaction databases

IntActiP01346. 1 interaction.
STRINGi10116.ENSRNOP00000047037.

Structurei

3D structure databases

ProteinModelPortaliP01346.
SMRiP01346. Positions 25-91.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni25 – 5228B
Add
BLAST
Regioni53 – 6412C
Add
BLAST
Regioni65 – 8521A
Add
BLAST
Regioni86 – 916D

Sequence similaritiesi

Belongs to the insulin family.

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG44017.
GeneTreeiENSGT00530000063856.
HOGENOMiHOG000233362.
HOVERGENiHBG006137.
InParanoidiP01346.
KOiK13769.
OrthoDBiEOG7TF7CG.
PhylomeDBiP01346.
TreeFamiTF332820.

Family and domain databases

Gene3Di1.10.100.10. 1 hit.
InterProiIPR022334. IGF2.
IPR013576. IGF2_C.
IPR016179. Insulin-like.
IPR022350. Insulin-like_growth_factor.
IPR022353. Insulin_CS.
IPR022352. Insulin_family.
[Graphical view]
PfamiPF08365. IGF2_C. 1 hit.
PF00049. Insulin. 1 hit.
[Graphical view]
PRINTSiPR02002. INSLNLIKEGF.
PR02006. INSLNLIKEGF2.
PR00276. INSULINFAMLY.
ProDomiPD005188. IGF2_C. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00078. IlGF. 1 hit.
[Graphical view]
SUPFAMiSSF56994. SSF56994. 1 hit.
PROSITEiPS00262. INSULIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P01346-1 [UniParc]FASTAAdd to Basket

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MGIPVGKSML VLLISLAFAL CCIAAYRPSE TLCGGELVDT LQFVCSDRGF    50
YFSRPSSRAN RRSRGIVEEC CFRSCDLALL ETYCATPAKS ERDVSTSQAV 100
LPDDFPRYPV GKFFKFDTWR QSAGRLRRGL PALLRARRGR MLAKELEAFR 150
EAKRHRPLIV LPPKDPAHGG ASSEMSSNHQ 180
Length:180
Mass (Da):20,086
Last modified:March 20, 1987 - v1
Checksum:iAF12B4EEC0DBCC34
GO

Sequence cautioni

The sequence AAB95624.1 differs from that shown. Reason: Erroneous initiation.
The sequence CAA25427.1 differs from that shown. Reason: Erroneous initiation.
The sequence CAA25429.1 differs from that shown. Reason: Erroneous initiation.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti1 – 88Missing1 Publication
Sequence conflicti57 – 571S → G1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X00911 mRNA. Translation: CAA25428.1.
X00911 mRNA. Translation: CAA25427.1. Different initiation.
X00911 mRNA. Translation: CAA25429.1. Different initiation.
M13871, M13869, M13870 Genomic DNA. Translation: AAB95624.1. Different initiation.
M29880, M29879 Genomic DNA. Translation: AAA41391.1.
X02213 mRNA. Translation: CAA26136.1.
X13101 mRNA. Translation: CAA31493.1.
X14833 mRNA. Translation: CAA32942.1.
X14834 mRNA. Translation: CAA32943.1.
M30273 mRNA. Translation: AAA41432.1.
M31221 Genomic DNA. Translation: AAA42046.1.
PIRiA25350. IGRT2.
RefSeqiNP_001177091.1. NM_001190162.1.
NP_001177092.1. NM_001190163.1.
NP_113699.2. NM_031511.2.
XP_006230727.1. XM_006230665.1.
UniGeneiRn.118681.
Rn.224460.

Genome annotation databases

EnsembliENSRNOT00000050760; ENSRNOP00000047037; ENSRNOG00000020369.
ENSRNOT00000073850; ENSRNOP00000066983; ENSRNOG00000020369.
GeneIDi24483.
KEGGirno:24483.
UCSCiRGD:2870. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X00911 mRNA. Translation: CAA25428.1 .
X00911 mRNA. Translation: CAA25427.1 . Different initiation.
X00911 mRNA. Translation: CAA25429.1 . Different initiation.
M13871 , M13869 , M13870 Genomic DNA. Translation: AAB95624.1 . Different initiation.
M29880 , M29879 Genomic DNA. Translation: AAA41391.1 .
X02213 mRNA. Translation: CAA26136.1 .
X13101 mRNA. Translation: CAA31493.1 .
X14833 mRNA. Translation: CAA32942.1 .
X14834 mRNA. Translation: CAA32943.1 .
M30273 mRNA. Translation: AAA41432.1 .
M31221 Genomic DNA. Translation: AAA42046.1 .
PIRi A25350. IGRT2.
RefSeqi NP_001177091.1. NM_001190162.1.
NP_001177092.1. NM_001190163.1.
NP_113699.2. NM_031511.2.
XP_006230727.1. XM_006230665.1.
UniGenei Rn.118681.
Rn.224460.

3D structure databases

ProteinModelPortali P01346.
SMRi P01346. Positions 25-91.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P01346. 1 interaction.
STRINGi 10116.ENSRNOP00000047037.

Proteomic databases

PaxDbi P01346.
PRIDEi P01346.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000050760 ; ENSRNOP00000047037 ; ENSRNOG00000020369 .
ENSRNOT00000073850 ; ENSRNOP00000066983 ; ENSRNOG00000020369 .
GeneIDi 24483.
KEGGi rno:24483.
UCSCi RGD:2870. rat.

Organism-specific databases

CTDi 3481.
RGDi 2870. Igf2.

Phylogenomic databases

eggNOGi NOG44017.
GeneTreei ENSGT00530000063856.
HOGENOMi HOG000233362.
HOVERGENi HBG006137.
InParanoidi P01346.
KOi K13769.
OrthoDBi EOG7TF7CG.
PhylomeDBi P01346.
TreeFami TF332820.

Enzyme and pathway databases

Reactomei REACT_194916. SHC-related events triggered by IGF1R.
REACT_208985. Signaling by Type 1 Insulin-like Growth Factor 1 Receptor (IGF1R).
REACT_222757. IRS-related events triggered by IGF1R.

Miscellaneous databases

NextBioi 603459.
PROi P01346.

Gene expression databases

ArrayExpressi P01346.
Genevestigatori P01346.

Family and domain databases

Gene3Di 1.10.100.10. 1 hit.
InterProi IPR022334. IGF2.
IPR013576. IGF2_C.
IPR016179. Insulin-like.
IPR022350. Insulin-like_growth_factor.
IPR022353. Insulin_CS.
IPR022352. Insulin_family.
[Graphical view ]
Pfami PF08365. IGF2_C. 1 hit.
PF00049. Insulin. 1 hit.
[Graphical view ]
PRINTSi PR02002. INSLNLIKEGF.
PR02006. INSLNLIKEGF2.
PR00276. INSULINFAMLY.
ProDomi PD005188. IGF2_C. 1 hit.
[Graphical view ] [Entries sharing at least one domain ]
SMARTi SM00078. IlGF. 1 hit.
[Graphical view ]
SUPFAMi SSF56994. SSF56994. 1 hit.
PROSITEi PS00262. INSULIN. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Insulin-like growth factor II precursor gene organization in relation to insulin gene family."
    Dull T.J., Gray A., Hayflick J.S., Ullrich A.
    Nature 310:777-781(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: BRL-3A.
  2. "Developmental and tissue-specific expression of a family of transcripts related to rat insulin-like growth factor II mRNA."
    Soares M.B., Ishii D.N., Efstratiadis A.
    Nucleic Acids Res. 13:1119-1134(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Buffalo.
  3. "Rat insulin-like growth factor II gene. A single gene with two promoters expressing a multitranscript family."
    Soares M.B., Turken A., Ishii D.N., Mills L., Episkopou V., Cotter S., Zeitlin S., Efstratiadis A.
    J. Mol. Biol. 192:737-752(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
  4. "Structure and expression of the rat insulin-like growth factor II (rIGF-II) gene. rIGF-II RNAs are transcribed from two promoters."
    Frunzio R., Chiariotti L., Brown A.L., Graham D.E., Rechler M.M., Bruni C.B.
    J. Biol. Chem. 261:17138-17149(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  5. "Transcriptional deviation of the rat insulin-like growth factor II gene initiated at three alternative leader-exons between neonatal tissues and ascites hepatomas."
    Ueno T., Takahashi K., Matsuguchi T., Endo H., Yamamoto M.
    Biochim. Biophys. Acta 950:411-419(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
  6. "Isolation of a cDNA clone encoding rat insulin-like growth factor-II precursor."
    Whitfield H.J., Bruni C.B., Frunzio R., Terrell J.E., Nissley S.P., Rechler M.M.
    Nature 312:277-280(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE OF 62-180.
  7. "Structure of the rat insulin-like growth factor II transcriptional unit: heterogeneous transcripts are generated from two promoters by use of multiple polyadenylation sites and differential ribonucleic acid splicing."
    Chiariotti L., Brown A.L., Frunzio R., Clemmons D.R., Rechler M.M., Bruni C.B.
    Mol. Endocrinol. 2:1115-1126(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE OF 103-180.
  8. "Purification and primary structure of a polypeptide with multiplication-stimulating activity from rat liver cell cultures. Homology with human insulin-like growth factor II."
    Marquardt H., Todaro G.J., Henderson L.E., Oroszlan S.
    J. Biol. Chem. 256:6859-6865(1981) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 25-91.
  9. "Preptin, another peptide product of the pancreatic beta-cell, is osteogenic in vitro and in vivo."
    Cornish J., Callon K.E., Bava U., Watson M., Xu X., Lin J.M., Chan V.A., Grey A.B., Naot D., Buchanan C.M., Cooper G.J., Reid I.R.
    Am. J. Physiol. 292:E117-E122(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: OSTEOGENIC FUNCTION OF PREPTIN.

Entry informationi

Entry nameiIGF2_RAT
AccessioniPrimary (citable) accession number: P01346
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: March 20, 1987
Last modified: September 3, 2014
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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