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Protein

Insulin-like growth factor II

Gene

Igf2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The insulin-like growth factors possess growth-promoting activity. In vitro, they are potent mitogens for cultured cells. IGF-II is influenced by placental lactogen and may play a role in fetal development.
Preptin undergoes glucose-mediated co-secretion with insulin, and acts as physiological amplifier of glucose-mediated insulin secretion. Exhibits osteogenic properties by increasing osteoblast mitogenic activity through phosphoactivation of MAPK1 and MAPK3.

GO - Molecular functioni

  1. growth factor activity Source: RGD
  2. protein serine/threonine kinase activator activity Source: Ensembl
  3. receptor activator activity Source: Ensembl

GO - Biological processi

  1. cell proliferation Source: RGD
  2. cellular response to mechanical stimulus Source: RGD
  3. exocrine pancreas development Source: Ensembl
  4. female pregnancy Source: RGD
  5. glucose metabolic process Source: UniProtKB-KW
  6. insulin receptor signaling pathway via phosphatidylinositol 3-kinase Source: Ensembl
  7. memory Source: RGD
  8. organ morphogenesis Source: Ensembl
  9. osteoblast differentiation Source: Ensembl
  10. positive regulation of activated T cell proliferation Source: Ensembl
  11. positive regulation of glycogen (starch) synthase activity Source: Ensembl
  12. positive regulation of glycogen biosynthetic process Source: Ensembl
  13. positive regulation of insulin receptor signaling pathway Source: Ensembl
  14. positive regulation of MAPK cascade Source: Ensembl
  15. positive regulation of mitosis Source: Ensembl
  16. positive regulation of peptidyl-tyrosine phosphorylation Source: Ensembl
  17. positive regulation of protein kinase B signaling Source: Ensembl
  18. positive regulation of steroid hormone biosynthetic process Source: BHF-UCL
  19. positive regulation of transcription from RNA polymerase II promoter Source: Ensembl
  20. response to drug Source: RGD
  21. response to estradiol Source: RGD
  22. response to ethanol Source: RGD
  23. response to nicotine Source: RGD
  24. response to nutrient levels Source: RGD
  25. response to organic cyclic compound Source: RGD
  26. response to radiation Source: RGD
  27. striated muscle cell differentiation Source: Ensembl
  28. wound healing Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Growth factor, Hormone, Mitogen

Keywords - Biological processi

Carbohydrate metabolism, Glucose metabolism, Osteogenesis

Enzyme and pathway databases

ReactomeiREACT_194916. SHC-related events triggered by IGF1R.
REACT_208985. Signaling by Type 1 Insulin-like Growth Factor 1 Receptor (IGF1R).
REACT_222757. IRS-related events triggered by IGF1R.
REACT_250882. Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs).

Names & Taxonomyi

Protein namesi
Recommended name:
Insulin-like growth factor II
Short name:
IGF-II
Alternative name(s):
Multiplication-stimulating activity
Short name:
MSA
Multiplication-stimulating polypeptide
Cleaved into the following 2 chains:
Gene namesi
Name:Igf2
Synonyms:Igf-2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 1

Organism-specific databases

RGDi2870. Igf2.

Subcellular locationi

GO - Cellular componenti

  1. extracellular space Source: RGD
  2. extracellular vesicular exosome Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 24241 PublicationAdd
BLAST
Chaini25 – 9167Insulin-like growth factor IIPRO_0000015729Add
BLAST
Propeptidei92 – 18089E peptidePRO_0000015730Add
BLAST
Peptidei93 – 12634PreptinPRO_0000370380Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi33 ↔ 71By similarity
Disulfide bondi45 ↔ 84By similarity
Disulfide bondi70 ↔ 75By similarity

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond

Proteomic databases

PaxDbiP01346.
PRIDEiP01346.

Expressioni

Gene expression databases

ExpressionAtlasiP01346. baseline and differential.
GenevestigatoriP01346.

Interactioni

Protein-protein interaction databases

IntActiP01346. 1 interaction.
STRINGi10116.ENSRNOP00000047037.

Structurei

3D structure databases

ProteinModelPortaliP01346.
SMRiP01346. Positions 25-91.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni25 – 5228BAdd
BLAST
Regioni53 – 6412CAdd
BLAST
Regioni65 – 8521AAdd
BLAST
Regioni86 – 916D

Sequence similaritiesi

Belongs to the insulin family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG44017.
GeneTreeiENSGT00530000063856.
HOGENOMiHOG000233362.
HOVERGENiHBG006137.
InParanoidiP01346.
KOiK13769.
OrthoDBiEOG7TF7CG.
PhylomeDBiP01346.
TreeFamiTF332820.

Family and domain databases

Gene3Di1.10.100.10. 1 hit.
InterProiIPR022334. IGF2.
IPR013576. IGF2_C.
IPR016179. Insulin-like.
IPR022350. Insulin-like_growth_factor.
IPR022353. Insulin_CS.
IPR022352. Insulin_family.
[Graphical view]
PfamiPF08365. IGF2_C. 1 hit.
PF00049. Insulin. 1 hit.
[Graphical view]
PRINTSiPR02002. INSLNLIKEGF.
PR02006. INSLNLIKEGF2.
PR00276. INSULINFAMLY.
ProDomiPD005188. IGF2_C. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00078. IlGF. 1 hit.
[Graphical view]
SUPFAMiSSF56994. SSF56994. 1 hit.
PROSITEiPS00262. INSULIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P01346-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGIPVGKSML VLLISLAFAL CCIAAYRPSE TLCGGELVDT LQFVCSDRGF
60 70 80 90 100
YFSRPSSRAN RRSRGIVEEC CFRSCDLALL ETYCATPAKS ERDVSTSQAV
110 120 130 140 150
LPDDFPRYPV GKFFKFDTWR QSAGRLRRGL PALLRARRGR MLAKELEAFR
160 170 180
EAKRHRPLIV LPPKDPAHGG ASSEMSSNHQ
Length:180
Mass (Da):20,086
Last modified:March 20, 1987 - v1
Checksum:iAF12B4EEC0DBCC34
GO

Sequence cautioni

The sequence AAB95624.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence CAA25427.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence CAA25429.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti1 – 88Missing(PubMed:3889836)Curated
Sequence conflicti57 – 571S → G(PubMed:2438416)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X00911 mRNA. Translation: CAA25428.1.
X00911 mRNA. Translation: CAA25427.1. Different initiation.
X00911 mRNA. Translation: CAA25429.1. Different initiation.
M13871, M13869, M13870 Genomic DNA. Translation: AAB95624.1. Different initiation.
M29880, M29879 Genomic DNA. Translation: AAA41391.1.
X02213 mRNA. Translation: CAA26136.1.
X13101 mRNA. Translation: CAA31493.1.
X14833 mRNA. Translation: CAA32942.1.
X14834 mRNA. Translation: CAA32943.1.
M30273 mRNA. Translation: AAA41432.1.
M31221 Genomic DNA. Translation: AAA42046.1.
PIRiA25350. IGRT2.
RefSeqiNP_001177091.1. NM_001190162.1.
NP_001177092.1. NM_001190163.1.
NP_113699.2. NM_031511.2.
XP_006230727.1. XM_006230665.2.
XP_008758297.1. XM_008760075.1.
UniGeneiRn.118681.
Rn.224460.

Genome annotation databases

EnsembliENSRNOT00000050760; ENSRNOP00000047037; ENSRNOG00000020369.
ENSRNOT00000073850; ENSRNOP00000066983; ENSRNOG00000020369.
GeneIDi24483.
KEGGirno:24483.
UCSCiRGD:2870. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X00911 mRNA. Translation: CAA25428.1.
X00911 mRNA. Translation: CAA25427.1. Different initiation.
X00911 mRNA. Translation: CAA25429.1. Different initiation.
M13871, M13869, M13870 Genomic DNA. Translation: AAB95624.1. Different initiation.
M29880, M29879 Genomic DNA. Translation: AAA41391.1.
X02213 mRNA. Translation: CAA26136.1.
X13101 mRNA. Translation: CAA31493.1.
X14833 mRNA. Translation: CAA32942.1.
X14834 mRNA. Translation: CAA32943.1.
M30273 mRNA. Translation: AAA41432.1.
M31221 Genomic DNA. Translation: AAA42046.1.
PIRiA25350. IGRT2.
RefSeqiNP_001177091.1. NM_001190162.1.
NP_001177092.1. NM_001190163.1.
NP_113699.2. NM_031511.2.
XP_006230727.1. XM_006230665.2.
XP_008758297.1. XM_008760075.1.
UniGeneiRn.118681.
Rn.224460.

3D structure databases

ProteinModelPortaliP01346.
SMRiP01346. Positions 25-91.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP01346. 1 interaction.
STRINGi10116.ENSRNOP00000047037.

Proteomic databases

PaxDbiP01346.
PRIDEiP01346.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000050760; ENSRNOP00000047037; ENSRNOG00000020369.
ENSRNOT00000073850; ENSRNOP00000066983; ENSRNOG00000020369.
GeneIDi24483.
KEGGirno:24483.
UCSCiRGD:2870. rat.

Organism-specific databases

CTDi3481.
RGDi2870. Igf2.

Phylogenomic databases

eggNOGiNOG44017.
GeneTreeiENSGT00530000063856.
HOGENOMiHOG000233362.
HOVERGENiHBG006137.
InParanoidiP01346.
KOiK13769.
OrthoDBiEOG7TF7CG.
PhylomeDBiP01346.
TreeFamiTF332820.

Enzyme and pathway databases

ReactomeiREACT_194916. SHC-related events triggered by IGF1R.
REACT_208985. Signaling by Type 1 Insulin-like Growth Factor 1 Receptor (IGF1R).
REACT_222757. IRS-related events triggered by IGF1R.
REACT_250882. Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs).

Miscellaneous databases

NextBioi603459.
PROiP01346.

Gene expression databases

ExpressionAtlasiP01346. baseline and differential.
GenevestigatoriP01346.

Family and domain databases

Gene3Di1.10.100.10. 1 hit.
InterProiIPR022334. IGF2.
IPR013576. IGF2_C.
IPR016179. Insulin-like.
IPR022350. Insulin-like_growth_factor.
IPR022353. Insulin_CS.
IPR022352. Insulin_family.
[Graphical view]
PfamiPF08365. IGF2_C. 1 hit.
PF00049. Insulin. 1 hit.
[Graphical view]
PRINTSiPR02002. INSLNLIKEGF.
PR02006. INSLNLIKEGF2.
PR00276. INSULINFAMLY.
ProDomiPD005188. IGF2_C. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00078. IlGF. 1 hit.
[Graphical view]
SUPFAMiSSF56994. SSF56994. 1 hit.
PROSITEiPS00262. INSULIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Insulin-like growth factor II precursor gene organization in relation to insulin gene family."
    Dull T.J., Gray A., Hayflick J.S., Ullrich A.
    Nature 310:777-781(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: BRL-3A.
  2. "Developmental and tissue-specific expression of a family of transcripts related to rat insulin-like growth factor II mRNA."
    Soares M.B., Ishii D.N., Efstratiadis A.
    Nucleic Acids Res. 13:1119-1134(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Buffalo.
  3. "Rat insulin-like growth factor II gene. A single gene with two promoters expressing a multitranscript family."
    Soares M.B., Turken A., Ishii D.N., Mills L., Episkopou V., Cotter S., Zeitlin S., Efstratiadis A.
    J. Mol. Biol. 192:737-752(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
  4. "Structure and expression of the rat insulin-like growth factor II (rIGF-II) gene. rIGF-II RNAs are transcribed from two promoters."
    Frunzio R., Chiariotti L., Brown A.L., Graham D.E., Rechler M.M., Bruni C.B.
    J. Biol. Chem. 261:17138-17149(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  5. "Transcriptional deviation of the rat insulin-like growth factor II gene initiated at three alternative leader-exons between neonatal tissues and ascites hepatomas."
    Ueno T., Takahashi K., Matsuguchi T., Endo H., Yamamoto M.
    Biochim. Biophys. Acta 950:411-419(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
  6. "Isolation of a cDNA clone encoding rat insulin-like growth factor-II precursor."
    Whitfield H.J., Bruni C.B., Frunzio R., Terrell J.E., Nissley S.P., Rechler M.M.
    Nature 312:277-280(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE OF 62-180.
  7. "Structure of the rat insulin-like growth factor II transcriptional unit: heterogeneous transcripts are generated from two promoters by use of multiple polyadenylation sites and differential ribonucleic acid splicing."
    Chiariotti L., Brown A.L., Frunzio R., Clemmons D.R., Rechler M.M., Bruni C.B.
    Mol. Endocrinol. 2:1115-1126(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE OF 103-180.
  8. "Purification and primary structure of a polypeptide with multiplication-stimulating activity from rat liver cell cultures. Homology with human insulin-like growth factor II."
    Marquardt H., Todaro G.J., Henderson L.E., Oroszlan S.
    J. Biol. Chem. 256:6859-6865(1981) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 25-91.
  9. "Preptin, another peptide product of the pancreatic beta-cell, is osteogenic in vitro and in vivo."
    Cornish J., Callon K.E., Bava U., Watson M., Xu X., Lin J.M., Chan V.A., Grey A.B., Naot D., Buchanan C.M., Cooper G.J., Reid I.R.
    Am. J. Physiol. 292:E117-E122(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: OSTEOGENIC FUNCTION OF PREPTIN.

Entry informationi

Entry nameiIGF2_RAT
AccessioniPrimary (citable) accession number: P01346
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: March 20, 1987
Last modified: January 7, 2015
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.