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P01346

- IGF2_RAT

UniProt

P01346 - IGF2_RAT

Protein

Insulin-like growth factor II

Gene

Igf2

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 127 (01 Oct 2014)
      Sequence version 1 (20 Mar 1987)
      Previous versions | rss
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    Functioni

    The insulin-like growth factors possess growth-promoting activity. In vitro, they are potent mitogens for cultured cells. IGF-II is influenced by placental lactogen and may play a role in fetal development.
    Preptin undergoes glucose-mediated co-secretion with insulin, and acts as physiological amplifier of glucose-mediated insulin secretion. Exhibits osteogenic properties by increasing osteoblast mitogenic activity through phosphoactivation of MAPK1 and MAPK3.

    GO - Molecular functioni

    1. growth factor activity Source: RGD
    2. protein binding Source: UniProtKB
    3. protein serine/threonine kinase activator activity Source: Ensembl
    4. receptor activator activity Source: Ensembl

    GO - Biological processi

    1. cell proliferation Source: RGD
    2. cellular response to mechanical stimulus Source: RGD
    3. exocrine pancreas development Source: Ensembl
    4. female pregnancy Source: RGD
    5. glucose metabolic process Source: UniProtKB-KW
    6. insulin receptor signaling pathway via phosphatidylinositol 3-kinase Source: Ensembl
    7. memory Source: RGD
    8. organ morphogenesis Source: Ensembl
    9. osteoblast differentiation Source: Ensembl
    10. positive regulation of activated T cell proliferation Source: Ensembl
    11. positive regulation of cell division Source: UniProtKB-KW
    12. positive regulation of glycogen (starch) synthase activity Source: Ensembl
    13. positive regulation of glycogen biosynthetic process Source: Ensembl
    14. positive regulation of insulin receptor signaling pathway Source: Ensembl
    15. positive regulation of MAPK cascade Source: Ensembl
    16. positive regulation of mitosis Source: Ensembl
    17. positive regulation of peptidyl-tyrosine phosphorylation Source: Ensembl
    18. positive regulation of protein kinase B signaling Source: Ensembl
    19. positive regulation of steroid hormone biosynthetic process Source: BHF-UCL
    20. positive regulation of transcription from RNA polymerase II promoter Source: Ensembl
    21. response to drug Source: RGD
    22. response to estradiol Source: RGD
    23. response to ethanol Source: RGD
    24. response to nicotine Source: RGD
    25. response to nutrient levels Source: RGD
    26. response to organic cyclic compound Source: RGD
    27. response to radiation Source: RGD
    28. striated muscle cell differentiation Source: Ensembl
    29. wound healing Source: RGD

    Keywords - Molecular functioni

    Growth factor, Hormone, Mitogen

    Keywords - Biological processi

    Carbohydrate metabolism, Glucose metabolism, Osteogenesis

    Enzyme and pathway databases

    ReactomeiREACT_194916. SHC-related events triggered by IGF1R.
    REACT_208985. Signaling by Type 1 Insulin-like Growth Factor 1 Receptor (IGF1R).
    REACT_222757. IRS-related events triggered by IGF1R.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Insulin-like growth factor II
    Short name:
    IGF-II
    Alternative name(s):
    Multiplication-stimulating activity
    Short name:
    MSA
    Multiplication-stimulating polypeptide
    Cleaved into the following 2 chains:
    Gene namesi
    Name:Igf2
    Synonyms:Igf-2
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 1

    Organism-specific databases

    RGDi2870. Igf2.

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular space Source: RGD

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 24241 PublicationAdd
    BLAST
    Chaini25 – 9167Insulin-like growth factor IIPRO_0000015729Add
    BLAST
    Propeptidei92 – 18089E peptidePRO_0000015730Add
    BLAST
    Peptidei93 – 12634PreptinPRO_0000370380Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi33 ↔ 71By similarity
    Disulfide bondi45 ↔ 84By similarity
    Disulfide bondi70 ↔ 75By similarity

    Keywords - PTMi

    Cleavage on pair of basic residues, Disulfide bond

    Proteomic databases

    PaxDbiP01346.
    PRIDEiP01346.

    Expressioni

    Gene expression databases

    ArrayExpressiP01346.
    GenevestigatoriP01346.

    Interactioni

    Protein-protein interaction databases

    IntActiP01346. 1 interaction.
    STRINGi10116.ENSRNOP00000047037.

    Structurei

    3D structure databases

    ProteinModelPortaliP01346.
    SMRiP01346. Positions 25-91.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni25 – 5228BAdd
    BLAST
    Regioni53 – 6412CAdd
    BLAST
    Regioni65 – 8521AAdd
    BLAST
    Regioni86 – 916D

    Sequence similaritiesi

    Belongs to the insulin family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG44017.
    GeneTreeiENSGT00530000063856.
    HOGENOMiHOG000233362.
    HOVERGENiHBG006137.
    InParanoidiP01346.
    KOiK13769.
    OrthoDBiEOG7TF7CG.
    PhylomeDBiP01346.
    TreeFamiTF332820.

    Family and domain databases

    Gene3Di1.10.100.10. 1 hit.
    InterProiIPR022334. IGF2.
    IPR013576. IGF2_C.
    IPR016179. Insulin-like.
    IPR022350. Insulin-like_growth_factor.
    IPR022353. Insulin_CS.
    IPR022352. Insulin_family.
    [Graphical view]
    PfamiPF08365. IGF2_C. 1 hit.
    PF00049. Insulin. 1 hit.
    [Graphical view]
    PRINTSiPR02002. INSLNLIKEGF.
    PR02006. INSLNLIKEGF2.
    PR00276. INSULINFAMLY.
    ProDomiPD005188. IGF2_C. 1 hit.
    [Graphical view] [Entries sharing at least one domain]
    SMARTiSM00078. IlGF. 1 hit.
    [Graphical view]
    SUPFAMiSSF56994. SSF56994. 1 hit.
    PROSITEiPS00262. INSULIN. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P01346-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGIPVGKSML VLLISLAFAL CCIAAYRPSE TLCGGELVDT LQFVCSDRGF    50
    YFSRPSSRAN RRSRGIVEEC CFRSCDLALL ETYCATPAKS ERDVSTSQAV 100
    LPDDFPRYPV GKFFKFDTWR QSAGRLRRGL PALLRARRGR MLAKELEAFR 150
    EAKRHRPLIV LPPKDPAHGG ASSEMSSNHQ 180
    Length:180
    Mass (Da):20,086
    Last modified:March 20, 1987 - v1
    Checksum:iAF12B4EEC0DBCC34
    GO

    Sequence cautioni

    The sequence AAB95624.1 differs from that shown. Reason: Erroneous initiation.
    The sequence CAA25427.1 differs from that shown. Reason: Erroneous initiation.
    The sequence CAA25429.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti1 – 88Missing(PubMed:3889836)Curated
    Sequence conflicti57 – 571S → G(PubMed:2438416)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X00911 mRNA. Translation: CAA25428.1.
    X00911 mRNA. Translation: CAA25427.1. Different initiation.
    X00911 mRNA. Translation: CAA25429.1. Different initiation.
    M13871, M13869, M13870 Genomic DNA. Translation: AAB95624.1. Different initiation.
    M29880, M29879 Genomic DNA. Translation: AAA41391.1.
    X02213 mRNA. Translation: CAA26136.1.
    X13101 mRNA. Translation: CAA31493.1.
    X14833 mRNA. Translation: CAA32942.1.
    X14834 mRNA. Translation: CAA32943.1.
    M30273 mRNA. Translation: AAA41432.1.
    M31221 Genomic DNA. Translation: AAA42046.1.
    PIRiA25350. IGRT2.
    RefSeqiNP_001177091.1. NM_001190162.1.
    NP_001177092.1. NM_001190163.1.
    NP_113699.2. NM_031511.2.
    XP_006230727.1. XM_006230665.1.
    UniGeneiRn.118681.
    Rn.224460.

    Genome annotation databases

    EnsembliENSRNOT00000050760; ENSRNOP00000047037; ENSRNOG00000020369.
    ENSRNOT00000073850; ENSRNOP00000066983; ENSRNOG00000020369.
    GeneIDi24483.
    KEGGirno:24483.
    UCSCiRGD:2870. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X00911 mRNA. Translation: CAA25428.1 .
    X00911 mRNA. Translation: CAA25427.1 . Different initiation.
    X00911 mRNA. Translation: CAA25429.1 . Different initiation.
    M13871 , M13869 , M13870 Genomic DNA. Translation: AAB95624.1 . Different initiation.
    M29880 , M29879 Genomic DNA. Translation: AAA41391.1 .
    X02213 mRNA. Translation: CAA26136.1 .
    X13101 mRNA. Translation: CAA31493.1 .
    X14833 mRNA. Translation: CAA32942.1 .
    X14834 mRNA. Translation: CAA32943.1 .
    M30273 mRNA. Translation: AAA41432.1 .
    M31221 Genomic DNA. Translation: AAA42046.1 .
    PIRi A25350. IGRT2.
    RefSeqi NP_001177091.1. NM_001190162.1.
    NP_001177092.1. NM_001190163.1.
    NP_113699.2. NM_031511.2.
    XP_006230727.1. XM_006230665.1.
    UniGenei Rn.118681.
    Rn.224460.

    3D structure databases

    ProteinModelPortali P01346.
    SMRi P01346. Positions 25-91.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P01346. 1 interaction.
    STRINGi 10116.ENSRNOP00000047037.

    Proteomic databases

    PaxDbi P01346.
    PRIDEi P01346.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000050760 ; ENSRNOP00000047037 ; ENSRNOG00000020369 .
    ENSRNOT00000073850 ; ENSRNOP00000066983 ; ENSRNOG00000020369 .
    GeneIDi 24483.
    KEGGi rno:24483.
    UCSCi RGD:2870. rat.

    Organism-specific databases

    CTDi 3481.
    RGDi 2870. Igf2.

    Phylogenomic databases

    eggNOGi NOG44017.
    GeneTreei ENSGT00530000063856.
    HOGENOMi HOG000233362.
    HOVERGENi HBG006137.
    InParanoidi P01346.
    KOi K13769.
    OrthoDBi EOG7TF7CG.
    PhylomeDBi P01346.
    TreeFami TF332820.

    Enzyme and pathway databases

    Reactomei REACT_194916. SHC-related events triggered by IGF1R.
    REACT_208985. Signaling by Type 1 Insulin-like Growth Factor 1 Receptor (IGF1R).
    REACT_222757. IRS-related events triggered by IGF1R.

    Miscellaneous databases

    NextBioi 603459.
    PROi P01346.

    Gene expression databases

    ArrayExpressi P01346.
    Genevestigatori P01346.

    Family and domain databases

    Gene3Di 1.10.100.10. 1 hit.
    InterProi IPR022334. IGF2.
    IPR013576. IGF2_C.
    IPR016179. Insulin-like.
    IPR022350. Insulin-like_growth_factor.
    IPR022353. Insulin_CS.
    IPR022352. Insulin_family.
    [Graphical view ]
    Pfami PF08365. IGF2_C. 1 hit.
    PF00049. Insulin. 1 hit.
    [Graphical view ]
    PRINTSi PR02002. INSLNLIKEGF.
    PR02006. INSLNLIKEGF2.
    PR00276. INSULINFAMLY.
    ProDomi PD005188. IGF2_C. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    SMARTi SM00078. IlGF. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56994. SSF56994. 1 hit.
    PROSITEi PS00262. INSULIN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Insulin-like growth factor II precursor gene organization in relation to insulin gene family."
      Dull T.J., Gray A., Hayflick J.S., Ullrich A.
      Nature 310:777-781(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE.
      Strain: BRL-3A.
    2. "Developmental and tissue-specific expression of a family of transcripts related to rat insulin-like growth factor II mRNA."
      Soares M.B., Ishii D.N., Efstratiadis A.
      Nucleic Acids Res. 13:1119-1134(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Buffalo.
    3. "Rat insulin-like growth factor II gene. A single gene with two promoters expressing a multitranscript family."
      Soares M.B., Turken A., Ishii D.N., Mills L., Episkopou V., Cotter S., Zeitlin S., Efstratiadis A.
      J. Mol. Biol. 192:737-752(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE.
    4. "Structure and expression of the rat insulin-like growth factor II (rIGF-II) gene. rIGF-II RNAs are transcribed from two promoters."
      Frunzio R., Chiariotti L., Brown A.L., Graham D.E., Rechler M.M., Bruni C.B.
      J. Biol. Chem. 261:17138-17149(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    5. "Transcriptional deviation of the rat insulin-like growth factor II gene initiated at three alternative leader-exons between neonatal tissues and ascites hepatomas."
      Ueno T., Takahashi K., Matsuguchi T., Endo H., Yamamoto M.
      Biochim. Biophys. Acta 950:411-419(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE.
    6. "Isolation of a cDNA clone encoding rat insulin-like growth factor-II precursor."
      Whitfield H.J., Bruni C.B., Frunzio R., Terrell J.E., Nissley S.P., Rechler M.M.
      Nature 312:277-280(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE OF 62-180.
    7. "Structure of the rat insulin-like growth factor II transcriptional unit: heterogeneous transcripts are generated from two promoters by use of multiple polyadenylation sites and differential ribonucleic acid splicing."
      Chiariotti L., Brown A.L., Frunzio R., Clemmons D.R., Rechler M.M., Bruni C.B.
      Mol. Endocrinol. 2:1115-1126(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE OF 103-180.
    8. "Purification and primary structure of a polypeptide with multiplication-stimulating activity from rat liver cell cultures. Homology with human insulin-like growth factor II."
      Marquardt H., Todaro G.J., Henderson L.E., Oroszlan S.
      J. Biol. Chem. 256:6859-6865(1981) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 25-91.
    9. "Preptin, another peptide product of the pancreatic beta-cell, is osteogenic in vitro and in vivo."
      Cornish J., Callon K.E., Bava U., Watson M., Xu X., Lin J.M., Chan V.A., Grey A.B., Naot D., Buchanan C.M., Cooper G.J., Reid I.R.
      Am. J. Physiol. 292:E117-E122(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: OSTEOGENIC FUNCTION OF PREPTIN.

    Entry informationi

    Entry nameiIGF2_RAT
    AccessioniPrimary (citable) accession number: P01346
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: March 20, 1987
    Last modified: October 1, 2014
    This is version 127 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3