ID   INS2_MOUSE              Reviewed;         110 AA.
AC   P01326;
DT   21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT   13-AUG-1987, sequence version 1.
DT   27-MAR-2024, entry version 204.
DE   RecName: Full=Insulin-2;
DE   Contains:
DE     RecName: Full=Insulin-2 B chain;
DE   Contains:
DE     RecName: Full=Insulin-2 A chain;
DE   Flags: Precursor;
GN   Name=Ins2; Synonyms=Ins-2;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3104603; DOI=10.1007/bf02100639;
RA   Wentworth B.M., Schaefer I.M., Villa-Komaroff L., Chirgwin J.M.;
RT   "Characterization of the two nonallelic genes encoding mouse
RT   preproinsulin.";
RL   J. Mol. Evol. 23:305-312(1986).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=NON;
RX   PubMed=2397023; DOI=10.1677/jme.0.0050061;
RA   Sawa T., Ohgaku S., Morioka H., Yano S.;
RT   "Molecular cloning and DNA sequence analysis of preproinsulin genes in the
RT   NON mouse, an animal model of human non-obese, non-insulin-dependent
RT   diabetes mellitus.";
RL   J. Mol. Endocrinol. 5:61-67(1990).
RN   [3]
RP   PROTEIN SEQUENCE OF 25-54 AND 90-110.
RX   PubMed=5063718; DOI=10.1515/bchm2.1972.353.1.451;
RA   Buenzli H.F., Glatthaar B., Kunz P., Muelhaupt E., Humbel R.E.;
RT   "Amino acid sequence of the two insulins from mouse (Maus musculus).";
RL   Hoppe-Seyler's Z. Physiol. Chem. 353:451-458(1972).
RN   [4]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Pancreas;
RX   PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA   Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA   Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT   "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL   Cell 143:1174-1189(2010).
CC   -!- FUNCTION: Insulin decreases blood glucose concentration. It increases
CC       cell permeability to monosaccharides, amino acids and fatty acids. It
CC       accelerates glycolysis, the pentose phosphate cycle, and glycogen
CC       synthesis in liver.
CC   -!- SUBUNIT: Heterodimer of a B chain and an A chain linked by two
CC       disulfide bonds. {ECO:0000250|UniProtKB:P01308}.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- SIMILARITY: Belongs to the insulin family. {ECO:0000305}.
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DR   EMBL; X04724; CAA28433.1; -; Genomic_DNA.
DR   CCDS; CCDS22034.1; -.
DR   PIR; A26342; INMS2.
DR   RefSeq; NP_001172012.1; NM_001185083.2.
DR   RefSeq; NP_001172013.1; NM_001185084.2.
DR   RefSeq; NP_032413.1; NM_008387.5.
DR   AlphaFoldDB; P01326; -.
DR   SMR; P01326; -.
DR   STRING; 10090.ENSMUSP00000147425; -.
DR   iPTMnet; P01326; -.
DR   PhosphoSitePlus; P01326; -.
DR   PaxDb; 10090-ENSMUSP00000101553; -.
DR   ProteomicsDB; 269317; -.
DR   ABCD; P01326; 1 sequenced antibody.
DR   DNASU; 16334; -.
DR   Ensembl; ENSMUST00000000220.3; ENSMUSP00000000220.3; ENSMUSG00000000215.12.
DR   Ensembl; ENSMUST00000105932.2; ENSMUSP00000101552.2; ENSMUSG00000000215.12.
DR   Ensembl; ENSMUST00000105933.8; ENSMUSP00000101553.2; ENSMUSG00000000215.12.
DR   Ensembl; ENSMUST00000105934.8; ENSMUSP00000101554.2; ENSMUSG00000000215.12.
DR   Ensembl; ENSMUST00000210288.2; ENSMUSP00000147425.2; ENSMUSG00000000215.12.
DR   GeneID; 16334; -.
DR   KEGG; mmu:16334; -.
DR   UCSC; uc009kof.2; mouse.
DR   AGR; MGI:96573; -.
DR   CTD; 16334; -.
DR   MGI; MGI:96573; Ins2.
DR   VEuPathDB; HostDB:ENSMUSG00000000215; -.
DR   eggNOG; ENOG502S5P5; Eukaryota.
DR   GeneTree; ENSGT00390000015440; -.
DR   InParanoid; P01326; -.
DR   OMA; CENTCSL; -.
DR   OrthoDB; 4213741at2759; -.
DR   PhylomeDB; P01326; -.
DR   TreeFam; TF332820; -.
DR   Reactome; R-MMU-264876; Insulin processing.
DR   Reactome; R-MMU-422085; Synthesis, secretion, and deacylation of Ghrelin.
DR   Reactome; R-MMU-6807878; COPI-mediated anterograde transport.
DR   Reactome; R-MMU-6811558; PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling.
DR   Reactome; R-MMU-74713; IRS activation.
DR   Reactome; R-MMU-74749; Signal attenuation.
DR   Reactome; R-MMU-74751; Insulin receptor signalling cascade.
DR   Reactome; R-MMU-74752; Signaling by Insulin receptor.
DR   Reactome; R-MMU-77387; Insulin receptor recycling.
DR   BioGRID-ORCS; 16334; 3 hits in 78 CRISPR screens.
DR   ChiTaRS; Ins2; mouse.
DR   PRO; PR:P01326; -.
DR   Proteomes; UP000000589; Chromosome 7.
DR   RNAct; P01326; Protein.
DR   Bgee; ENSMUSG00000000215; Expressed in islet of Langerhans and 59 other cell types or tissues.
DR   ExpressionAtlas; P01326; baseline and differential.
DR   GO; GO:0062023; C:collagen-containing extracellular matrix; HDA:BHF-UCL.
DR   GO; GO:0005737; C:cytoplasm; IDA:MGI.
DR   GO; GO:0005829; C:cytosol; IDA:MGI.
DR   GO; GO:0005788; C:endoplasmic reticulum lumen; TAS:Reactome.
DR   GO; GO:0005576; C:extracellular region; TAS:Reactome.
DR   GO; GO:0005615; C:extracellular space; IDA:MGI.
DR   GO; GO:0005634; C:nucleus; IDA:MGI.
DR   GO; GO:0030141; C:secretory granule; IDA:MGI.
DR   GO; GO:0034774; C:secretory granule lumen; TAS:Reactome.
DR   GO; GO:0005732; C:sno(s)RNA-containing ribonucleoprotein complex; IDA:MGI.
DR   GO; GO:0005179; F:hormone activity; ISO:MGI.
DR   GO; GO:0042802; F:identical protein binding; ISO:MGI.
DR   GO; GO:0005158; F:insulin receptor binding; ISO:MGI.
DR   GO; GO:0005159; F:insulin-like growth factor receptor binding; ISO:MGI.
DR   GO; GO:0002020; F:protease binding; ISO:MGI.
DR   GO; GO:0048018; F:receptor ligand activity; ISO:MGI.
DR   GO; GO:0030297; F:transmembrane receptor protein tyrosine kinase activator activity; ISO:MGI.
DR   GO; GO:0008270; F:zinc ion binding; IGI:MGI.
DR   GO; GO:0006953; P:acute-phase response; ISO:MGI.
DR   GO; GO:0046631; P:alpha-beta T cell activation; ISO:MGI.
DR   GO; GO:0006983; P:ER overload response; IMP:MGI.
DR   GO; GO:0055089; P:fatty acid homeostasis; ISO:MGI.
DR   GO; GO:0007186; P:G protein-coupled receptor signaling pathway; ISO:MGI.
DR   GO; GO:0042593; P:glucose homeostasis; IMP:MGI.
DR   GO; GO:0006006; P:glucose metabolic process; IMP:MGI.
DR   GO; GO:1904659; P:glucose transmembrane transport; TAS:MGI.
DR   GO; GO:0030070; P:insulin processing; IGI:MGI.
DR   GO; GO:0008286; P:insulin receptor signaling pathway; IDA:MGI.
DR   GO; GO:0070059; P:intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress; IMP:MGI.
DR   GO; GO:0016042; P:lipid catabolic process; IDA:MGI.
DR   GO; GO:0007520; P:myoblast fusion; IGI:MGI.
DR   GO; GO:0014902; P:myotube differentiation; IGI:MGI.
DR   GO; GO:0002674; P:negative regulation of acute inflammatory response; ISO:MGI.
DR   GO; GO:0045922; P:negative regulation of fatty acid metabolic process; ISO:MGI.
DR   GO; GO:2000252; P:negative regulation of feeding behavior; ISO:MGI.
DR   GO; GO:0010629; P:negative regulation of gene expression; ISO:MGI.
DR   GO; GO:0045818; P:negative regulation of glycogen catabolic process; ISO:MGI.
DR   GO; GO:0050995; P:negative regulation of lipid catabolic process; ISO:MGI.
DR   GO; GO:0042177; P:negative regulation of protein catabolic process; ISO:MGI.
DR   GO; GO:0050709; P:negative regulation of protein secretion; ISO:MGI.
DR   GO; GO:0045861; P:negative regulation of proteolysis; ISO:MGI.
DR   GO; GO:1903427; P:negative regulation of reactive oxygen species biosynthetic process; ISO:MGI.
DR   GO; GO:0060266; P:negative regulation of respiratory burst involved in inflammatory response; ISO:MGI.
DR   GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IDA:MGI.
DR   GO; GO:1990535; P:neuron projection maintenance; ISO:MGI.
DR   GO; GO:0038060; P:nitric oxide-cGMP-mediated signaling pathway; ISO:MGI.
DR   GO; GO:0008284; P:positive regulation of cell population proliferation; ISO:MGI.
DR   GO; GO:0001819; P:positive regulation of cytokine production; ISO:MGI.
DR   GO; GO:1902952; P:positive regulation of dendritic spine maintenance; ISO:MGI.
DR   GO; GO:0070374; P:positive regulation of ERK1 and ERK2 cascade; IDA:MGI.
DR   GO; GO:0010628; P:positive regulation of gene expression; ISO:MGI.
DR   GO; GO:0046326; P:positive regulation of glucose import; ISO:MGI.
DR   GO; GO:0045725; P:positive regulation of glycogen biosynthetic process; ISO:MGI.
DR   GO; GO:0045821; P:positive regulation of glycolytic process; ISO:MGI.
DR   GO; GO:0046628; P:positive regulation of insulin receptor signaling pathway; ISO:MGI.
DR   GO; GO:0043410; P:positive regulation of MAPK cascade; ISO:MGI.
DR   GO; GO:0045840; P:positive regulation of mitotic nuclear division; ISO:MGI.
DR   GO; GO:0010750; P:positive regulation of nitric oxide mediated signal transduction; ISO:MGI.
DR   GO; GO:0050731; P:positive regulation of peptidyl-tyrosine phosphorylation; IDA:MGI.
DR   GO; GO:0051897; P:positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction; IDA:MGI.
DR   GO; GO:1900182; P:positive regulation of protein localization to nucleus; ISO:MGI.
DR   GO; GO:0051247; P:positive regulation of protein metabolic process; ISO:MGI.
DR   GO; GO:0050714; P:positive regulation of protein secretion; IBA:GO_Central.
DR   GO; GO:0060267; P:positive regulation of respiratory burst; ISO:MGI.
DR   GO; GO:0031623; P:receptor internalization; IDA:MGI.
DR   GO; GO:0006521; P:regulation of cellular amino acid metabolic process; ISO:MGI.
DR   GO; GO:0010468; P:regulation of gene expression; IDA:MGI.
DR   GO; GO:0042325; P:regulation of phosphorylation; IDA:MGI.
DR   GO; GO:0032880; P:regulation of protein localization; ISO:MGI.
DR   GO; GO:1903076; P:regulation of protein localization to plasma membrane; ISO:MGI.
DR   GO; GO:0050708; P:regulation of protein secretion; ISO:MGI.
DR   GO; GO:0042311; P:vasodilation; ISO:MGI.
DR   GO; GO:0042060; P:wound healing; ISO:MGI.
DR   CDD; cd04367; IlGF_insulin_like; 1.
DR   Gene3D; 1.10.100.10; Insulin-like; 1.
DR   InterPro; IPR004825; Insulin.
DR   InterPro; IPR016179; Insulin-like.
DR   InterPro; IPR036438; Insulin-like_sf.
DR   InterPro; IPR022353; Insulin_CS.
DR   InterPro; IPR022352; Insulin_family.
DR   PANTHER; PTHR11454:SF33; INSULIN-2; 1.
DR   PANTHER; PTHR11454; INSULIN/INSULIN GROWTH FACTOR; 1.
DR   Pfam; PF00049; Insulin; 1.
DR   PRINTS; PR00277; INSULIN.
DR   PRINTS; PR00276; INSULINFAMLY.
DR   SMART; SM00078; IlGF; 1.
DR   SUPFAM; SSF56994; Insulin-like; 1.
DR   PROSITE; PS00262; INSULIN; 1.
DR   Genevisible; P01326; MM.
PE   1: Evidence at protein level;
KW   Carbohydrate metabolism; Cleavage on pair of basic residues;
KW   Direct protein sequencing; Disulfide bond; Glucose metabolism; Hormone;
KW   Reference proteome; Secreted; Signal.
FT   SIGNAL          1..24
FT                   /evidence="ECO:0000269|PubMed:5063718"
FT   PEPTIDE         25..54
FT                   /note="Insulin-2 B chain"
FT                   /evidence="ECO:0000269|PubMed:3104603"
FT                   /id="PRO_0000015845"
FT   PROPEP          57..87
FT                   /note="C peptide"
FT                   /id="PRO_0000015846"
FT   PEPTIDE         90..110
FT                   /note="Insulin-2 A chain"
FT                   /evidence="ECO:0000269|PubMed:3104603"
FT                   /id="PRO_0000015847"
FT   DISULFID        31..96
FT                   /note="Interchain (between B and A chains)"
FT                   /evidence="ECO:0000250|UniProtKB:P01308"
FT   DISULFID        43..109
FT                   /note="Interchain (between B and A chains)"
FT                   /evidence="ECO:0000250|UniProtKB:P01308"
FT   DISULFID        95..100
FT                   /evidence="ECO:0000250|UniProtKB:P01308"
SQ   SEQUENCE   110 AA;  12364 MW;  3554C8803D24FDAD CRC64;
     MALWMRFLPL LALLFLWESH PTQAFVKQHL CGSHLVEALY LVCGERGFFY TPMSRREVED
     PQVAQLELGG GPGAGDLQTL ALEVAQQKRG IVDQCCTSIC SLYQLENYCN
//