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P01326

- INS2_MOUSE

UniProt

P01326 - INS2_MOUSE

Protein

Insulin-2

Gene

Ins2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.

    GO - Biological processi

    1. activation of MAPK activity Source: MGI
    2. canonical Wnt signaling pathway Source: MGI
    3. ER overload response Source: MGI
    4. glucose metabolic process Source: MGI
    5. glucose transport Source: MGI
    6. insulin receptor signaling pathway Source: MGI
    7. intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress Source: MGI
    8. lipid catabolic process Source: MGI
    9. myoblast fusion Source: MGI
    10. myotube differentiation Source: MGI
    11. negative regulation of transcription from RNA polymerase II promoter Source: MGI
    12. positive regulation of phosphatidylinositol 3-kinase activity Source: MGI
    13. regulation of gene expression Source: MGI
    14. regulation of phosphorylation Source: MGI
    15. regulation of protein binding Source: MGI

    Keywords - Molecular functioni

    Hormone

    Keywords - Biological processi

    Carbohydrate metabolism, Glucose metabolism

    Enzyme and pathway databases

    ReactomeiREACT_198563. Amyloids.
    REACT_207837. Insulin processing.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Insulin-2
    Cleaved into the following 2 chains:
    Gene namesi
    Name:Ins2
    Synonyms:Ins-2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 7

    Organism-specific databases

    MGIiMGI:96573. Ins2.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: MGI
    2. extracellular region Source: Reactome
    3. extracellular space Source: Ensembl
    4. nucleus Source: MGI
    5. secretory granule Source: MGI
    6. secretory granule lumen Source: Reactome
    7. small nucleolar ribonucleoprotein complex Source: MGI

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 24241 PublicationAdd
    BLAST
    Peptidei25 – 5430Insulin-2 B chain1 PublicationPRO_0000015845Add
    BLAST
    Propeptidei57 – 8731C peptidePRO_0000015846Add
    BLAST
    Peptidei90 – 11021Insulin-2 A chain1 PublicationPRO_0000015847Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi31 ↔ 96Interchain (between B and A chains)
    Disulfide bondi43 ↔ 109Interchain (between B and A chains)
    Disulfide bondi95 ↔ 100

    Keywords - PTMi

    Cleavage on pair of basic residues, Disulfide bond

    Proteomic databases

    PaxDbiP01326.
    PRIDEiP01326.

    PTM databases

    PhosphoSiteiP01326.

    Miscellaneous databases

    PMAP-CutDBP01326.

    Expressioni

    Gene expression databases

    ArrayExpressiP01326.
    BgeeiP01326.
    CleanExiMM_INS2.
    GenevestigatoriP01326.

    Interactioni

    Subunit structurei

    Heterodimer of a B chain and an A chain linked by two disulfide bonds.

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000101554.

    Structurei

    3D structure databases

    ProteinModelPortaliP01326.
    SMRiP01326. Positions 25-110.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the insulin family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG45999.
    HOGENOMiHOG000261669.
    HOVERGENiHBG006137.
    InParanoidiP01326.
    KOiK04526.
    OMAiVEQCCHN.
    PhylomeDBiP01326.
    TreeFamiTF332820.

    Family and domain databases

    Gene3Di1.10.100.10. 1 hit.
    InterProiIPR004825. Insulin.
    IPR016179. Insulin-like.
    IPR022353. Insulin_CS.
    IPR022352. Insulin_family.
    [Graphical view]
    PfamiPF00049. Insulin. 1 hit.
    [Graphical view]
    PRINTSiPR00277. INSULIN.
    PR00276. INSULINFAMLY.
    SMARTiSM00078. IlGF. 1 hit.
    [Graphical view]
    SUPFAMiSSF56994. SSF56994. 1 hit.
    PROSITEiPS00262. INSULIN. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P01326-1 [UniParc]FASTAAdd to Basket

    « Hide

    MALWMRFLPL LALLFLWESH PTQAFVKQHL CGSHLVEALY LVCGERGFFY    50
    TPMSRREVED PQVAQLELGG GPGAGDLQTL ALEVAQQKRG IVDQCCTSIC 100
    SLYQLENYCN 110
    Length:110
    Mass (Da):12,364
    Last modified:August 13, 1987 - v1
    Checksum:i3554C8803D24FDAD
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X04724 Genomic DNA. Translation: CAA28433.1.
    CCDSiCCDS22034.1.
    PIRiA26342. INMS2.
    RefSeqiNP_001172012.1. NM_001185083.1.
    NP_001172013.1. NM_001185084.1.
    NP_032413.1. NM_008387.4.
    UniGeneiMm.4946.

    Genome annotation databases

    EnsembliENSMUST00000000220; ENSMUSP00000000220; ENSMUSG00000000215.
    ENSMUST00000105932; ENSMUSP00000101552; ENSMUSG00000000215.
    ENSMUST00000105933; ENSMUSP00000101553; ENSMUSG00000000215.
    ENSMUST00000105934; ENSMUSP00000101554; ENSMUSG00000000215.
    GeneIDi16334.
    KEGGimmu:16334.
    UCSCiuc009kof.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X04724 Genomic DNA. Translation: CAA28433.1 .
    CCDSi CCDS22034.1.
    PIRi A26342. INMS2.
    RefSeqi NP_001172012.1. NM_001185083.1.
    NP_001172013.1. NM_001185084.1.
    NP_032413.1. NM_008387.4.
    UniGenei Mm.4946.

    3D structure databases

    ProteinModelPortali P01326.
    SMRi P01326. Positions 25-110.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10090.ENSMUSP00000101554.

    PTM databases

    PhosphoSitei P01326.

    Proteomic databases

    PaxDbi P01326.
    PRIDEi P01326.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000000220 ; ENSMUSP00000000220 ; ENSMUSG00000000215 .
    ENSMUST00000105932 ; ENSMUSP00000101552 ; ENSMUSG00000000215 .
    ENSMUST00000105933 ; ENSMUSP00000101553 ; ENSMUSG00000000215 .
    ENSMUST00000105934 ; ENSMUSP00000101554 ; ENSMUSG00000000215 .
    GeneIDi 16334.
    KEGGi mmu:16334.
    UCSCi uc009kof.2. mouse.

    Organism-specific databases

    CTDi 16334.
    MGIi MGI:96573. Ins2.

    Phylogenomic databases

    eggNOGi NOG45999.
    HOGENOMi HOG000261669.
    HOVERGENi HBG006137.
    InParanoidi P01326.
    KOi K04526.
    OMAi VEQCCHN.
    PhylomeDBi P01326.
    TreeFami TF332820.

    Enzyme and pathway databases

    Reactomei REACT_198563. Amyloids.
    REACT_207837. Insulin processing.

    Miscellaneous databases

    ChiTaRSi Ins2. mouse.
    NextBioi 289430.
    PMAP-CutDB P01326.
    PROi P01326.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P01326.
    Bgeei P01326.
    CleanExi MM_INS2.
    Genevestigatori P01326.

    Family and domain databases

    Gene3Di 1.10.100.10. 1 hit.
    InterProi IPR004825. Insulin.
    IPR016179. Insulin-like.
    IPR022353. Insulin_CS.
    IPR022352. Insulin_family.
    [Graphical view ]
    Pfami PF00049. Insulin. 1 hit.
    [Graphical view ]
    PRINTSi PR00277. INSULIN.
    PR00276. INSULINFAMLY.
    SMARTi SM00078. IlGF. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56994. SSF56994. 1 hit.
    PROSITEi PS00262. INSULIN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Characterization of the two nonallelic genes encoding mouse preproinsulin."
      Wentworth B.M., Schaefer I.M., Villa-Komaroff L., Chirgwin J.M.
      J. Mol. Evol. 23:305-312(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Molecular cloning and DNA sequence analysis of preproinsulin genes in the NON mouse, an animal model of human non-obese, non-insulin-dependent diabetes mellitus."
      Sawa T., Ohgaku S., Morioka H., Yano S.
      J. Mol. Endocrinol. 5:61-67(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: NON.
    3. "Amino acid sequence of the two insulins from mouse (Maus musculus)."
      Buenzli H.F., Glatthaar B., Kunz P., Muelhaupt E., Humbel R.E.
      Hoppe-Seyler's Z. Physiol. Chem. 353:451-458(1972) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 25-54 AND 90-110.

    Entry informationi

    Entry nameiINS2_MOUSE
    AccessioniPrimary (citable) accession number: P01326
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: August 13, 1987
    Last modified: October 1, 2014
    This is version 139 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3