Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P01326

- INS2_MOUSE

UniProt

P01326 - INS2_MOUSE

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Insulin-2

Gene

Ins2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.

GO - Biological processi

  1. activation of MAPK activity Source: MGI
  2. canonical Wnt signaling pathway Source: MGI
  3. ER overload response Source: MGI
  4. glucose metabolic process Source: MGI
  5. glucose transport Source: MGI
  6. insulin receptor signaling pathway Source: MGI
  7. intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress Source: MGI
  8. lipid catabolic process Source: MGI
  9. myoblast fusion Source: MGI
  10. myotube differentiation Source: MGI
  11. negative regulation of transcription from RNA polymerase II promoter Source: MGI
  12. positive regulation of phosphatidylinositol 3-kinase activity Source: MGI
  13. regulation of gene expression Source: MGI
  14. regulation of phosphorylation Source: MGI
  15. regulation of protein binding Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Hormone

Keywords - Biological processi

Carbohydrate metabolism, Glucose metabolism

Enzyme and pathway databases

ReactomeiREACT_198563. Amyloids.
REACT_207837. Insulin processing.
REACT_233181. Insulin receptor recycling.
REACT_233293. Synthesis, secretion, and deacylation of Ghrelin.
REACT_240250. Signaling by Insulin receptor.
REACT_244029. Signal attenuation.
REACT_246380. SHC activation.
REACT_261478. IRS activation.
REACT_263148. Regulation of insulin secretion.

Names & Taxonomyi

Protein namesi
Recommended name:
Insulin-2
Cleaved into the following 2 chains:
Gene namesi
Name:Ins2
Synonyms:Ins-2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 7

Organism-specific databases

MGIiMGI:96573. Ins2.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: MGI
  2. extracellular region Source: Reactome
  3. extracellular space Source: Ensembl
  4. nucleus Source: MGI
  5. secretory granule Source: MGI
  6. secretory granule lumen Source: Reactome
  7. small nucleolar ribonucleoprotein complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 24241 PublicationAdd
BLAST
Peptidei25 – 5430Insulin-2 B chain1 PublicationPRO_0000015845Add
BLAST
Propeptidei57 – 8731C peptidePRO_0000015846Add
BLAST
Peptidei90 – 11021Insulin-2 A chain1 PublicationPRO_0000015847Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi31 ↔ 96Interchain (between B and A chains)
Disulfide bondi43 ↔ 109Interchain (between B and A chains)
Disulfide bondi95 ↔ 100

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond

Proteomic databases

PaxDbiP01326.
PRIDEiP01326.

PTM databases

PhosphoSiteiP01326.

Miscellaneous databases

PMAP-CutDBP01326.

Expressioni

Gene expression databases

BgeeiP01326.
CleanExiMM_INS2.
ExpressionAtlasiP01326. baseline and differential.
GenevestigatoriP01326.

Interactioni

Subunit structurei

Heterodimer of a B chain and an A chain linked by two disulfide bonds.

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000101554.

Structurei

3D structure databases

ProteinModelPortaliP01326.
SMRiP01326. Positions 25-110.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the insulin family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG45999.
HOGENOMiHOG000261669.
HOVERGENiHBG006137.
InParanoidiP01326.
KOiK04526.
OMAiVEQCCHN.
PhylomeDBiP01326.
TreeFamiTF332820.

Family and domain databases

Gene3Di1.10.100.10. 1 hit.
InterProiIPR004825. Insulin.
IPR016179. Insulin-like.
IPR022353. Insulin_CS.
IPR022352. Insulin_family.
[Graphical view]
PfamiPF00049. Insulin. 1 hit.
[Graphical view]
PRINTSiPR00277. INSULIN.
PR00276. INSULINFAMLY.
SMARTiSM00078. IlGF. 1 hit.
[Graphical view]
SUPFAMiSSF56994. SSF56994. 1 hit.
PROSITEiPS00262. INSULIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P01326-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MALWMRFLPL LALLFLWESH PTQAFVKQHL CGSHLVEALY LVCGERGFFY
60 70 80 90 100
TPMSRREVED PQVAQLELGG GPGAGDLQTL ALEVAQQKRG IVDQCCTSIC
110
SLYQLENYCN
Length:110
Mass (Da):12,364
Last modified:August 13, 1987 - v1
Checksum:i3554C8803D24FDAD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04724 Genomic DNA. Translation: CAA28433.1.
CCDSiCCDS22034.1.
PIRiA26342. INMS2.
RefSeqiNP_001172012.1. NM_001185083.1.
NP_001172013.1. NM_001185084.1.
NP_032413.1. NM_008387.4.
UniGeneiMm.4946.

Genome annotation databases

EnsembliENSMUST00000000220; ENSMUSP00000000220; ENSMUSG00000000215.
ENSMUST00000105932; ENSMUSP00000101552; ENSMUSG00000000215.
ENSMUST00000105933; ENSMUSP00000101553; ENSMUSG00000000215.
ENSMUST00000105934; ENSMUSP00000101554; ENSMUSG00000000215.
GeneIDi16334.
KEGGimmu:16334.
UCSCiuc009kof.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04724 Genomic DNA. Translation: CAA28433.1 .
CCDSi CCDS22034.1.
PIRi A26342. INMS2.
RefSeqi NP_001172012.1. NM_001185083.1.
NP_001172013.1. NM_001185084.1.
NP_032413.1. NM_008387.4.
UniGenei Mm.4946.

3D structure databases

ProteinModelPortali P01326.
SMRi P01326. Positions 25-110.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10090.ENSMUSP00000101554.

PTM databases

PhosphoSitei P01326.

Proteomic databases

PaxDbi P01326.
PRIDEi P01326.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000000220 ; ENSMUSP00000000220 ; ENSMUSG00000000215 .
ENSMUST00000105932 ; ENSMUSP00000101552 ; ENSMUSG00000000215 .
ENSMUST00000105933 ; ENSMUSP00000101553 ; ENSMUSG00000000215 .
ENSMUST00000105934 ; ENSMUSP00000101554 ; ENSMUSG00000000215 .
GeneIDi 16334.
KEGGi mmu:16334.
UCSCi uc009kof.2. mouse.

Organism-specific databases

CTDi 16334.
MGIi MGI:96573. Ins2.

Phylogenomic databases

eggNOGi NOG45999.
HOGENOMi HOG000261669.
HOVERGENi HBG006137.
InParanoidi P01326.
KOi K04526.
OMAi VEQCCHN.
PhylomeDBi P01326.
TreeFami TF332820.

Enzyme and pathway databases

Reactomei REACT_198563. Amyloids.
REACT_207837. Insulin processing.
REACT_233181. Insulin receptor recycling.
REACT_233293. Synthesis, secretion, and deacylation of Ghrelin.
REACT_240250. Signaling by Insulin receptor.
REACT_244029. Signal attenuation.
REACT_246380. SHC activation.
REACT_261478. IRS activation.
REACT_263148. Regulation of insulin secretion.

Miscellaneous databases

ChiTaRSi Ins2. mouse.
NextBioi 289430.
PMAP-CutDB P01326.
PROi P01326.
SOURCEi Search...

Gene expression databases

Bgeei P01326.
CleanExi MM_INS2.
ExpressionAtlasi P01326. baseline and differential.
Genevestigatori P01326.

Family and domain databases

Gene3Di 1.10.100.10. 1 hit.
InterProi IPR004825. Insulin.
IPR016179. Insulin-like.
IPR022353. Insulin_CS.
IPR022352. Insulin_family.
[Graphical view ]
Pfami PF00049. Insulin. 1 hit.
[Graphical view ]
PRINTSi PR00277. INSULIN.
PR00276. INSULINFAMLY.
SMARTi SM00078. IlGF. 1 hit.
[Graphical view ]
SUPFAMi SSF56994. SSF56994. 1 hit.
PROSITEi PS00262. INSULIN. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Characterization of the two nonallelic genes encoding mouse preproinsulin."
    Wentworth B.M., Schaefer I.M., Villa-Komaroff L., Chirgwin J.M.
    J. Mol. Evol. 23:305-312(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Molecular cloning and DNA sequence analysis of preproinsulin genes in the NON mouse, an animal model of human non-obese, non-insulin-dependent diabetes mellitus."
    Sawa T., Ohgaku S., Morioka H., Yano S.
    J. Mol. Endocrinol. 5:61-67(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: NON.
  3. "Amino acid sequence of the two insulins from mouse (Maus musculus)."
    Buenzli H.F., Glatthaar B., Kunz P., Muelhaupt E., Humbel R.E.
    Hoppe-Seyler's Z. Physiol. Chem. 353:451-458(1972) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 25-54 AND 90-110.

Entry informationi

Entry nameiINS2_MOUSE
AccessioniPrimary (citable) accession number: P01326
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: August 13, 1987
Last modified: November 26, 2014
This is version 141 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3