P01326 (INS2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Insulin-2

Cleaved into the following 2 chains:

1. Insulin-2 B chain
2. Insulin-2 A chain

Gene names

Name:	Ins2
Synonyms:	Ins-2

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	110 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
Subunit structure	Heterodimer of a B chain and an A chain linked by two disulfide bonds.
Subcellular location	Secreted.
Sequence similarities	Belongs to the insulin family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Glucose metabolism
Cellular component	Secreted
Domain	Signal
Molecular function	Hormone
PTM	Cleavage on pair of basic residues Disulfide bond
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	ER overload response Inferred from mutant phenotype PubMed 11854325. Source: MGI activation of MAPK activity Inferred from direct assay PubMed 12059784. Source: MGI canonical Wnt receptor signaling pathway Inferred from genetic interaction PubMed 15282335. Source: MGI glucose metabolic process Inferred from mutant phenotype PubMed 11854325. Source: MGI glucose transport Traceable author statement PubMed 12297296. Source: MGI insulin receptor signaling pathway Inferred from direct assay PubMed 12805218. Source: MGI lipid catabolic process Inferred from direct assay PubMed 12805218. Source: MGI myoblast fusion Inferred from genetic interaction PubMed 15282335. Source: MGI negative regulation of transcription from RNA polymerase II promoter Inferred from direct assay PubMed 9689059. Source: MGI positive regulation of phosphatidylinositol 3-kinase activity Inferred from direct assay PubMed 11375348. Source: MGI regulation of apoptotic process Inferred from mutant phenotype PubMed 11854325. Source: MGI regulation of protein binding Inferred from direct assay PubMed 9507006. Source: MGI
Cellular_component	extracellular region Traceable author statement. Source: Reactome extracellular space Inferred from electronic annotation. Source: Compara nucleus Inferred from direct assay PubMed 12488434. Source: MGI secretory granule Inferred from direct assay PubMed 15788565. Source: MGI small nucleolar ribonucleoprotein complex Inferred from direct assay PubMed 12488434. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24	Ref.3
	Peptide	25 – 54	30	Insulin-2 B chain Ref.1 Ref.3		PRO_0000015845
	Propeptide	57 – 87	31	C peptide		PRO_0000015846
	Peptide	90 – 110	21	Insulin-2 A chain Ref.1		PRO_0000015847
Amino acid modifications
	Disulfide bond	31 ↔ 96		Interchain (between B and A chains)
	Disulfide bond	43 ↔ 109		Interchain (between B and A chains)
	Disulfide bond	95 ↔ 100

Sequences

	Sequence		Length	Mass (Da)
	P01326 [UniParc]. Last modified August 13, 1987. Version 1. Checksum: 3554C8803D24FDAD	FASTA	110	12,364
	10 20 30 40 50 60 MALWMRFLPL LALLFLWESH PTQAFVKQHL CGSHLVEALY LVCGERGFFY TPMSRREVED 70 80 90 100 110 PQVAQLELGG GPGAGDLQTL ALEVAQQKRG IVDQCCTSIC SLYQLENYCN

References

[1]	"Characterization of the two nonallelic genes encoding mouse preproinsulin." Wentworth B.M., Schaefer I.M., Villa-Komaroff L., Chirgwin J.M. J. Mol. Evol. 23:305-312(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Molecular cloning and DNA sequence analysis of preproinsulin genes in the NON mouse, an animal model of human non-obese, non-insulin-dependent diabetes mellitus." Sawa T., Ohgaku S., Morioka H., Yano S. J. Mol. Endocrinol. 5:61-67(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: NON.
[3]	"Amino acid sequence of the two insulins from mouse (Maus musculus)." Buenzli H.F., Glatthaar B., Kunz P., Muelhaupt E., Humbel R.E. Hoppe-Seyler's Z. Physiol. Chem. 353:451-458(1972) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 25-54 AND 90-110.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X04724 Genomic DNA. Translation: CAA28433.1.
IPI	IPI00134311.
PIR	INMS2. A26342.
RefSeq	NP_001172012.1. NM_001185083.1. NP_001172013.1. NM_001185084.1. NP_032413.1. NM_008387.4.
UniGene	Mm.4946.
3D structure databases
ProteinModelPortal	P01326.
SMR	P01326. Positions 25-110.
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000101554.
PTM databases
PhosphoSite	P01326.
Proteomic databases
PaxDb	P01326.
PRIDE	P01326.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000000220; ENSMUSP00000000220; ENSMUSG00000000215. ENSMUST00000105932; ENSMUSP00000101552; ENSMUSG00000000215. ENSMUST00000105933; ENSMUSP00000101553; ENSMUSG00000000215. ENSMUST00000105934; ENSMUSP00000101554; ENSMUSG00000000215.
GeneID	16334.
KEGG	mmu:16334.
Organism-specific databases
CTD	16334.
MGI	MGI:96573. Ins2.
Phylogenomic databases
eggNOG	NOG45999.
HOGENOM	HOG000261669.
HOVERGEN	HBG006137.
InParanoid	P01326.
KO	K04526.
OMA	AMAPPQH.
OrthoDB	EOG4RNB9Q.
Enzyme and pathway databases
Reactome	REACT_112621. Metabolism. REACT_118324. Disease.
Gene expression databases
ArrayExpress	P01326.
Bgee	P01326.
CleanEx	MM_INS2.
Genevestigator	P01326.
GermOnline	ENSMUSG00000000215. Mus musculus.
Family and domain databases
Gene3D	1.10.100.10. 1 hit.
InterPro	IPR004825. Insulin. IPR016179. Insulin-like. IPR022353. Insulin_CS. IPR022352. Insulin_family. [Graphical view]
Pfam	PF00049. Insulin. 1 hit. [Graphical view]
PRINTS	PR00277. INSULIN. PR00276. INSULINFAMLY.
SMART	SM00078. IlGF. 1 hit. [Graphical view]
SUPFAM	SSF56994. Insulin-like. 1 hit.
PROSITE	PS00262. INSULIN. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	Ins2. mouse.
NextBio	289430.
PMAP-CutDB	P01326.
SOURCE	Search...

Entry information

Entry name

INS2_MOUSE

Accession

Primary (citable) accession number: P01326

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	August 13, 1987
Last modified:	April 3, 2013
This is version 126 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program