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P01326 (INS2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 135. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Insulin-2

Cleaved into the following 2 chains:

  1. Insulin-2 B chain
  2. Insulin-2 A chain
Gene names
Name:Ins2
Synonyms:Ins-2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length110 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.

Subunit structure

Heterodimer of a B chain and an A chain linked by two disulfide bonds.

Subcellular location

Secreted.

Sequence similarities

Belongs to the insulin family.

Ontologies

Keywords
   Biological processCarbohydrate metabolism
Glucose metabolism
   Cellular componentSecreted
   DomainSignal
   Molecular functionHormone
   PTMCleavage on pair of basic residues
Disulfide bond
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processER overload response

Inferred from mutant phenotype PubMed 11854325. Source: MGI

activation of MAPK activity

Inferred from direct assay PubMed 12059784. Source: MGI

canonical Wnt signaling pathway

Inferred from genetic interaction PubMed 15282335. Source: MGI

glucose metabolic process

Inferred from mutant phenotype PubMed 11854325. Source: MGI

glucose transport

Traceable author statement PubMed 12297296. Source: MGI

insulin receptor signaling pathway

Inferred from direct assay PubMed 12805218. Source: MGI

intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress

Inferred from mutant phenotype PubMed 11854325. Source: MGI

lipid catabolic process

Inferred from direct assay PubMed 12805218. Source: MGI

myoblast fusion

Inferred from genetic interaction PubMed 15282335. Source: MGI

myotube differentiation

Inferred from genetic interaction PubMed 15282335. Source: MGI

negative regulation of transcription from RNA polymerase II promoter

Inferred from direct assay PubMed 9689059. Source: MGI

positive regulation of phosphatidylinositol 3-kinase activity

Inferred from direct assay PubMed 11375348. Source: MGI

regulation of gene expression

Inferred from direct assay PubMed 19293336. Source: MGI

regulation of phosphorylation

Inferred from direct assay PubMed 12059784. Source: MGI

regulation of protein binding

Inferred from direct assay PubMed 9507006. Source: MGI

   Cellular_componentcytoplasm

Inferred from direct assay PubMed 15793245. Source: MGI

extracellular region

Traceable author statement. Source: Reactome

extracellular space

Inferred from electronic annotation. Source: Ensembl

nucleus

Inferred from direct assay PubMed 12488434. Source: MGI

secretory granule

Inferred from direct assay PubMed 15788565. Source: MGI

secretory granule lumen

Traceable author statement. Source: Reactome

small nucleolar ribonucleoprotein complex

Inferred from direct assay PubMed 12488434. Source: MGI

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2424 Ref.3
Peptide25 – 5430Insulin-2 B chain Ref.1 Ref.3
PRO_0000015845
Propeptide57 – 8731C peptide
PRO_0000015846
Peptide90 – 11021Insulin-2 A chain Ref.1
PRO_0000015847

Amino acid modifications

Disulfide bond31 ↔ 96Interchain (between B and A chains)
Disulfide bond43 ↔ 109Interchain (between B and A chains)
Disulfide bond95 ↔ 100

Sequences

Sequence LengthMass (Da)Tools
P01326 [UniParc].

Last modified August 13, 1987. Version 1.
Checksum: 3554C8803D24FDAD

FASTA11012,364
        10         20         30         40         50         60 
MALWMRFLPL LALLFLWESH PTQAFVKQHL CGSHLVEALY LVCGERGFFY TPMSRREVED 

        70         80         90        100        110 
PQVAQLELGG GPGAGDLQTL ALEVAQQKRG IVDQCCTSIC SLYQLENYCN 

« Hide

References

[1]"Characterization of the two nonallelic genes encoding mouse preproinsulin."
Wentworth B.M., Schaefer I.M., Villa-Komaroff L., Chirgwin J.M.
J. Mol. Evol. 23:305-312(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Molecular cloning and DNA sequence analysis of preproinsulin genes in the NON mouse, an animal model of human non-obese, non-insulin-dependent diabetes mellitus."
Sawa T., Ohgaku S., Morioka H., Yano S.
J. Mol. Endocrinol. 5:61-67(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: NON.
[3]"Amino acid sequence of the two insulins from mouse (Maus musculus)."
Buenzli H.F., Glatthaar B., Kunz P., Muelhaupt E., Humbel R.E.
Hoppe-Seyler's Z. Physiol. Chem. 353:451-458(1972) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 25-54 AND 90-110.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X04724 Genomic DNA. Translation: CAA28433.1.
PIRINMS2. A26342.
RefSeqNP_001172012.1. NM_001185083.1.
NP_001172013.1. NM_001185084.1.
NP_032413.1. NM_008387.4.
UniGeneMm.4946.

3D structure databases

ProteinModelPortalP01326.
SMRP01326. Positions 25-110.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000101554.

PTM databases

PhosphoSiteP01326.

Proteomic databases

PaxDbP01326.
PRIDEP01326.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000000220; ENSMUSP00000000220; ENSMUSG00000000215.
ENSMUST00000105932; ENSMUSP00000101552; ENSMUSG00000000215.
ENSMUST00000105933; ENSMUSP00000101553; ENSMUSG00000000215.
ENSMUST00000105934; ENSMUSP00000101554; ENSMUSG00000000215.
GeneID16334.
KEGGmmu:16334.
UCSCuc009kof.2. mouse.

Organism-specific databases

CTD16334.
MGIMGI:96573. Ins2.

Phylogenomic databases

eggNOGNOG45999.
HOGENOMHOG000261669.
HOVERGENHBG006137.
InParanoidP01326.
KOK04526.
OMAAPPQHLC.
PhylomeDBP01326.
TreeFamTF332820.

Enzyme and pathway databases

ReactomeREACT_188937. Metabolism.
REACT_93132. Metabolism of proteins.

Gene expression databases

ArrayExpressP01326.
BgeeP01326.
CleanExMM_INS2.
GenevestigatorP01326.

Family and domain databases

Gene3D1.10.100.10. 1 hit.
InterProIPR004825. Insulin.
IPR016179. Insulin-like.
IPR022353. Insulin_CS.
IPR022352. Insulin_family.
[Graphical view]
PfamPF00049. Insulin. 1 hit.
[Graphical view]
PRINTSPR00277. INSULIN.
PR00276. INSULINFAMLY.
SMARTSM00078. IlGF. 1 hit.
[Graphical view]
SUPFAMSSF56994. SSF56994. 1 hit.
PROSITEPS00262. INSULIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSIns2. mouse.
NextBio289430.
PMAP-CutDBP01326.
PROP01326.
SOURCESearch...

Entry information

Entry nameINS2_MOUSE
AccessionPrimary (citable) accession number: P01326
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: August 13, 1987
Last modified: April 16, 2014
This is version 135 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot