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Protein

Insulin-2

Gene

Ins2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hormone

Keywords - Biological processi

Carbohydrate metabolism, Glucose metabolism

Enzyme and pathway databases

ReactomeiREACT_284329. Signaling by Insulin receptor.
REACT_298481. Insulin processing.
REACT_304877. IRS activation.
REACT_306614. Regulation of insulin secretion.
REACT_324664. Insulin receptor recycling.
REACT_326726. SHC activation.
REACT_330516. Synthesis, secretion, and deacylation of Ghrelin.
REACT_333431. Signal attenuation.

Names & Taxonomyi

Protein namesi
Recommended name:
Insulin-2
Cleaved into the following 2 chains:
Gene namesi
Name:Ins2
Synonyms:Ins-2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:96573. Ins2.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: MGI
  • cytosol Source: MGI
  • extracellular region Source: Reactome
  • extracellular space Source: MGI
  • nucleus Source: MGI
  • secretory granule Source: MGI
  • secretory granule lumen Source: Reactome
  • small nucleolar ribonucleoprotein complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 24241 PublicationAdd
BLAST
Peptidei25 – 5430Insulin-2 B chain1 PublicationPRO_0000015845Add
BLAST
Propeptidei57 – 8731C peptidePRO_0000015846Add
BLAST
Peptidei90 – 11021Insulin-2 A chain1 PublicationPRO_0000015847Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi31 ↔ 96Interchain (between B and A chains)
Disulfide bondi43 ↔ 109Interchain (between B and A chains)
Disulfide bondi95 ↔ 100

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond

Proteomic databases

PaxDbiP01326.
PRIDEiP01326.

PTM databases

PhosphoSiteiP01326.

Miscellaneous databases

PMAP-CutDBP01326.

Expressioni

Gene expression databases

BgeeiP01326.
CleanExiMM_INS2.
ExpressionAtlasiP01326. differential.
GenevisibleiP01326. MM.

Interactioni

Subunit structurei

Heterodimer of a B chain and an A chain linked by two disulfide bonds.

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000000220.

Structurei

3D structure databases

ProteinModelPortaliP01326.
SMRiP01326. Positions 25-110.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the insulin family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG45999.
HOGENOMiHOG000261669.
HOVERGENiHBG006137.
InParanoidiP01326.
KOiK04526.
OMAiAPPQHLC.
PhylomeDBiP01326.
TreeFamiTF332820.

Family and domain databases

Gene3Di1.10.100.10. 1 hit.
InterProiIPR004825. Insulin.
IPR016179. Insulin-like.
IPR022353. Insulin_CS.
IPR022352. Insulin_family.
[Graphical view]
PfamiPF00049. Insulin. 1 hit.
[Graphical view]
PRINTSiPR00277. INSULIN.
PR00276. INSULINFAMLY.
SMARTiSM00078. IlGF. 1 hit.
[Graphical view]
SUPFAMiSSF56994. SSF56994. 1 hit.
PROSITEiPS00262. INSULIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P01326-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALWMRFLPL LALLFLWESH PTQAFVKQHL CGSHLVEALY LVCGERGFFY
60 70 80 90 100
TPMSRREVED PQVAQLELGG GPGAGDLQTL ALEVAQQKRG IVDQCCTSIC
110
SLYQLENYCN
Length:110
Mass (Da):12,364
Last modified:August 13, 1987 - v1
Checksum:i3554C8803D24FDAD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04724 Genomic DNA. Translation: CAA28433.1.
CCDSiCCDS22034.1.
PIRiA26342. INMS2.
RefSeqiNP_001172012.1. NM_001185083.1.
NP_001172013.1. NM_001185084.1.
NP_032413.1. NM_008387.4.
UniGeneiMm.4946.

Genome annotation databases

EnsembliENSMUST00000000220; ENSMUSP00000000220; ENSMUSG00000000215.
ENSMUST00000105932; ENSMUSP00000101552; ENSMUSG00000000215.
ENSMUST00000105933; ENSMUSP00000101553; ENSMUSG00000000215.
ENSMUST00000105934; ENSMUSP00000101554; ENSMUSG00000000215.
GeneIDi16334.
KEGGimmu:16334.
UCSCiuc009kof.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04724 Genomic DNA. Translation: CAA28433.1.
CCDSiCCDS22034.1.
PIRiA26342. INMS2.
RefSeqiNP_001172012.1. NM_001185083.1.
NP_001172013.1. NM_001185084.1.
NP_032413.1. NM_008387.4.
UniGeneiMm.4946.

3D structure databases

ProteinModelPortaliP01326.
SMRiP01326. Positions 25-110.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000000220.

PTM databases

PhosphoSiteiP01326.

Proteomic databases

PaxDbiP01326.
PRIDEiP01326.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000000220; ENSMUSP00000000220; ENSMUSG00000000215.
ENSMUST00000105932; ENSMUSP00000101552; ENSMUSG00000000215.
ENSMUST00000105933; ENSMUSP00000101553; ENSMUSG00000000215.
ENSMUST00000105934; ENSMUSP00000101554; ENSMUSG00000000215.
GeneIDi16334.
KEGGimmu:16334.
UCSCiuc009kof.2. mouse.

Organism-specific databases

CTDi16334.
MGIiMGI:96573. Ins2.

Phylogenomic databases

eggNOGiNOG45999.
HOGENOMiHOG000261669.
HOVERGENiHBG006137.
InParanoidiP01326.
KOiK04526.
OMAiAPPQHLC.
PhylomeDBiP01326.
TreeFamiTF332820.

Enzyme and pathway databases

ReactomeiREACT_284329. Signaling by Insulin receptor.
REACT_298481. Insulin processing.
REACT_304877. IRS activation.
REACT_306614. Regulation of insulin secretion.
REACT_324664. Insulin receptor recycling.
REACT_326726. SHC activation.
REACT_330516. Synthesis, secretion, and deacylation of Ghrelin.
REACT_333431. Signal attenuation.

Miscellaneous databases

ChiTaRSiIns2. mouse.
NextBioi289430.
PMAP-CutDBP01326.
PROiP01326.
SOURCEiSearch...

Gene expression databases

BgeeiP01326.
CleanExiMM_INS2.
ExpressionAtlasiP01326. differential.
GenevisibleiP01326. MM.

Family and domain databases

Gene3Di1.10.100.10. 1 hit.
InterProiIPR004825. Insulin.
IPR016179. Insulin-like.
IPR022353. Insulin_CS.
IPR022352. Insulin_family.
[Graphical view]
PfamiPF00049. Insulin. 1 hit.
[Graphical view]
PRINTSiPR00277. INSULIN.
PR00276. INSULINFAMLY.
SMARTiSM00078. IlGF. 1 hit.
[Graphical view]
SUPFAMiSSF56994. SSF56994. 1 hit.
PROSITEiPS00262. INSULIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Characterization of the two nonallelic genes encoding mouse preproinsulin."
    Wentworth B.M., Schaefer I.M., Villa-Komaroff L., Chirgwin J.M.
    J. Mol. Evol. 23:305-312(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Molecular cloning and DNA sequence analysis of preproinsulin genes in the NON mouse, an animal model of human non-obese, non-insulin-dependent diabetes mellitus."
    Sawa T., Ohgaku S., Morioka H., Yano S.
    J. Mol. Endocrinol. 5:61-67(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: NON.
  3. "Amino acid sequence of the two insulins from mouse (Maus musculus)."
    Buenzli H.F., Glatthaar B., Kunz P., Muelhaupt E., Humbel R.E.
    Hoppe-Seyler's Z. Physiol. Chem. 353:451-458(1972) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 25-54 AND 90-110.

Entry informationi

Entry nameiINS2_MOUSE
AccessioniPrimary (citable) accession number: P01326
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: August 13, 1987
Last modified: June 24, 2015
This is version 147 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.