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Protein

Insulin-2

Gene

Ins2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.

GO - Molecular functioni

  1. hormone activity Source: MGI
  2. identical protein binding Source: MGI
  3. insulin-like growth factor receptor binding Source: MGI
  4. insulin receptor binding Source: MGI
  5. protease binding Source: MGI

GO - Biological processi

  1. activation of MAPK activity Source: MGI
  2. activation of protein kinase B activity Source: MGI
  3. acute-phase response Source: MGI
  4. alpha-beta T cell activation Source: MGI
  5. canonical Wnt signaling pathway Source: MGI
  6. ER overload response Source: MGI
  7. fatty acid homeostasis Source: MGI
  8. glucose homeostasis Source: MGI
  9. glucose metabolic process Source: MGI
  10. glucose transport Source: MGI
  11. G-protein coupled receptor signaling pathway Source: MGI
  12. insulin receptor signaling pathway Source: MGI
  13. intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress Source: MGI
  14. lipid catabolic process Source: MGI
  15. MAPK cascade Source: MGI
  16. myoblast fusion Source: MGI
  17. myotube differentiation Source: MGI
  18. negative regulation of acute inflammatory response Source: MGI
  19. negative regulation of fatty acid metabolic process Source: MGI
  20. negative regulation of feeding behavior Source: MGI
  21. negative regulation of glycogen catabolic process Source: MGI
  22. negative regulation of lipid catabolic process Source: MGI
  23. negative regulation of NAD(P)H oxidase activity Source: MGI
  24. negative regulation of protein catabolic process Source: MGI
  25. negative regulation of protein oligomerization Source: MGI
  26. negative regulation of protein secretion Source: MGI
  27. negative regulation of proteolysis Source: MGI
  28. negative regulation of respiratory burst involved in inflammatory response Source: MGI
  29. negative regulation of transcription from RNA polymerase II promoter Source: MGI
  30. positive regulation of cell proliferation Source: MGI
  31. positive regulation of cellular protein metabolic process Source: MGI
  32. positive regulation of cytokine secretion Source: MGI
  33. positive regulation of DNA replication Source: MGI
  34. positive regulation of glucose import Source: MGI
  35. positive regulation of glycogen biosynthetic process Source: MGI
  36. positive regulation of glycolytic process Source: MGI
  37. positive regulation of insulin receptor signaling pathway Source: MGI
  38. positive regulation of MAPK cascade Source: MGI
  39. positive regulation of mitotic nuclear division Source: MGI
  40. positive regulation of NF-kappaB transcription factor activity Source: MGI
  41. positive regulation of peptidyl-tyrosine phosphorylation Source: MGI
  42. positive regulation of phosphatidylinositol 3-kinase activity Source: MGI
  43. positive regulation of phosphatidylinositol 3-kinase signaling Source: MGI
  44. positive regulation of protein kinase B signaling Source: MGI
  45. positive regulation of protein localization to nucleus Source: MGI
  46. positive regulation of respiratory burst Source: MGI
  47. regulation of cellular amino acid metabolic process Source: MGI
  48. regulation of gene expression Source: MGI
  49. regulation of phosphorylation Source: MGI
  50. regulation of protein binding Source: MGI
  51. regulation of protein localization Source: MGI
  52. regulation of protein secretion Source: MGI
  53. regulation of transmembrane transporter activity Source: MGI
  54. wound healing Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Hormone

Keywords - Biological processi

Carbohydrate metabolism, Glucose metabolism

Enzyme and pathway databases

ReactomeiREACT_284329. Signaling by Insulin receptor.
REACT_298481. Insulin processing.
REACT_304877. IRS activation.
REACT_306614. Regulation of insulin secretion.
REACT_324664. Insulin receptor recycling.
REACT_326726. SHC activation.
REACT_330516. Synthesis, secretion, and deacylation of Ghrelin.
REACT_333431. Signal attenuation.
REACT_342573. Amyloids.

Names & Taxonomyi

Protein namesi
Recommended name:
Insulin-2
Cleaved into the following 2 chains:
Gene namesi
Name:Ins2
Synonyms:Ins-2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:96573. Ins2.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: MGI
  2. extracellular region Source: Reactome
  3. extracellular space Source: MGI
  4. nucleus Source: MGI
  5. secretory granule Source: MGI
  6. secretory granule lumen Source: Reactome
  7. small nucleolar ribonucleoprotein complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 24241 PublicationAdd
BLAST
Peptidei25 – 5430Insulin-2 B chain1 PublicationPRO_0000015845Add
BLAST
Propeptidei57 – 8731C peptidePRO_0000015846Add
BLAST
Peptidei90 – 11021Insulin-2 A chain1 PublicationPRO_0000015847Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi31 ↔ 96Interchain (between B and A chains)
Disulfide bondi43 ↔ 109Interchain (between B and A chains)
Disulfide bondi95 ↔ 100

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond

Proteomic databases

PaxDbiP01326.
PRIDEiP01326.

PTM databases

PhosphoSiteiP01326.

Miscellaneous databases

PMAP-CutDBP01326.

Expressioni

Gene expression databases

BgeeiP01326.
CleanExiMM_INS2.
ExpressionAtlasiP01326. differential.
GenevestigatoriP01326.

Interactioni

Subunit structurei

Heterodimer of a B chain and an A chain linked by two disulfide bonds.

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000101554.

Structurei

3D structure databases

ProteinModelPortaliP01326.
SMRiP01326. Positions 25-110.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the insulin family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG45999.
HOGENOMiHOG000261669.
HOVERGENiHBG006137.
InParanoidiP01326.
KOiK04526.
OMAiAPPQHLC.
PhylomeDBiP01326.
TreeFamiTF332820.

Family and domain databases

Gene3Di1.10.100.10. 1 hit.
InterProiIPR004825. Insulin.
IPR016179. Insulin-like.
IPR022353. Insulin_CS.
IPR022352. Insulin_family.
[Graphical view]
PfamiPF00049. Insulin. 1 hit.
[Graphical view]
PRINTSiPR00277. INSULIN.
PR00276. INSULINFAMLY.
SMARTiSM00078. IlGF. 1 hit.
[Graphical view]
SUPFAMiSSF56994. SSF56994. 1 hit.
PROSITEiPS00262. INSULIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P01326-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALWMRFLPL LALLFLWESH PTQAFVKQHL CGSHLVEALY LVCGERGFFY
60 70 80 90 100
TPMSRREVED PQVAQLELGG GPGAGDLQTL ALEVAQQKRG IVDQCCTSIC
110
SLYQLENYCN
Length:110
Mass (Da):12,364
Last modified:August 13, 1987 - v1
Checksum:i3554C8803D24FDAD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04724 Genomic DNA. Translation: CAA28433.1.
CCDSiCCDS22034.1.
PIRiA26342. INMS2.
RefSeqiNP_001172012.1. NM_001185083.1.
NP_001172013.1. NM_001185084.1.
NP_032413.1. NM_008387.4.
UniGeneiMm.4946.

Genome annotation databases

EnsembliENSMUST00000000220; ENSMUSP00000000220; ENSMUSG00000000215.
ENSMUST00000105932; ENSMUSP00000101552; ENSMUSG00000000215.
ENSMUST00000105933; ENSMUSP00000101553; ENSMUSG00000000215.
ENSMUST00000105934; ENSMUSP00000101554; ENSMUSG00000000215.
GeneIDi16334.
KEGGimmu:16334.
UCSCiuc009kof.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04724 Genomic DNA. Translation: CAA28433.1.
CCDSiCCDS22034.1.
PIRiA26342. INMS2.
RefSeqiNP_001172012.1. NM_001185083.1.
NP_001172013.1. NM_001185084.1.
NP_032413.1. NM_008387.4.
UniGeneiMm.4946.

3D structure databases

ProteinModelPortaliP01326.
SMRiP01326. Positions 25-110.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000101554.

PTM databases

PhosphoSiteiP01326.

Proteomic databases

PaxDbiP01326.
PRIDEiP01326.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000000220; ENSMUSP00000000220; ENSMUSG00000000215.
ENSMUST00000105932; ENSMUSP00000101552; ENSMUSG00000000215.
ENSMUST00000105933; ENSMUSP00000101553; ENSMUSG00000000215.
ENSMUST00000105934; ENSMUSP00000101554; ENSMUSG00000000215.
GeneIDi16334.
KEGGimmu:16334.
UCSCiuc009kof.2. mouse.

Organism-specific databases

CTDi16334.
MGIiMGI:96573. Ins2.

Phylogenomic databases

eggNOGiNOG45999.
HOGENOMiHOG000261669.
HOVERGENiHBG006137.
InParanoidiP01326.
KOiK04526.
OMAiAPPQHLC.
PhylomeDBiP01326.
TreeFamiTF332820.

Enzyme and pathway databases

ReactomeiREACT_284329. Signaling by Insulin receptor.
REACT_298481. Insulin processing.
REACT_304877. IRS activation.
REACT_306614. Regulation of insulin secretion.
REACT_324664. Insulin receptor recycling.
REACT_326726. SHC activation.
REACT_330516. Synthesis, secretion, and deacylation of Ghrelin.
REACT_333431. Signal attenuation.
REACT_342573. Amyloids.

Miscellaneous databases

ChiTaRSiIns2. mouse.
NextBioi289430.
PMAP-CutDBP01326.
PROiP01326.
SOURCEiSearch...

Gene expression databases

BgeeiP01326.
CleanExiMM_INS2.
ExpressionAtlasiP01326. differential.
GenevestigatoriP01326.

Family and domain databases

Gene3Di1.10.100.10. 1 hit.
InterProiIPR004825. Insulin.
IPR016179. Insulin-like.
IPR022353. Insulin_CS.
IPR022352. Insulin_family.
[Graphical view]
PfamiPF00049. Insulin. 1 hit.
[Graphical view]
PRINTSiPR00277. INSULIN.
PR00276. INSULINFAMLY.
SMARTiSM00078. IlGF. 1 hit.
[Graphical view]
SUPFAMiSSF56994. SSF56994. 1 hit.
PROSITEiPS00262. INSULIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Characterization of the two nonallelic genes encoding mouse preproinsulin."
    Wentworth B.M., Schaefer I.M., Villa-Komaroff L., Chirgwin J.M.
    J. Mol. Evol. 23:305-312(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Molecular cloning and DNA sequence analysis of preproinsulin genes in the NON mouse, an animal model of human non-obese, non-insulin-dependent diabetes mellitus."
    Sawa T., Ohgaku S., Morioka H., Yano S.
    J. Mol. Endocrinol. 5:61-67(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: NON.
  3. "Amino acid sequence of the two insulins from mouse (Maus musculus)."
    Buenzli H.F., Glatthaar B., Kunz P., Muelhaupt E., Humbel R.E.
    Hoppe-Seyler's Z. Physiol. Chem. 353:451-458(1972) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 25-54 AND 90-110.

Entry informationi

Entry nameiINS2_MOUSE
AccessioniPrimary (citable) accession number: P01326
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: August 13, 1987
Last modified: April 1, 2015
This is version 145 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.