Reviewed, UniProtKB/Swiss-Prot P01321 (INS_CANFA)

Names and origin

Protein names

Recommended name:
Insulin

Cleaved into the following 2 chains:

1. Insulin B chain
2. Insulin A chain

Gene names

Name:

INS

Organism

Canis familiaris (Dog)

Taxonomic identifier

9615 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Carnivora › Caniformia › Canidae › Canis

Protein attributes

Sequence length	110 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
Subunit structure	Heterodimer of a B chain and an A chain linked by two disulfide bonds.
Subcellular location	Secreted.
Sequence similarities	Belongs to the insulin family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Glucose metabolism
Cellular component	Secreted
Domain	Signal
Molecular function	Hormone
PTM	Cleavage on pair of basic residues Disulfide bond
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	glucose metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	hormone activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24	Ref.2
	Peptide	25 – 54	30	Insulin B chain Ref.2		PRO_0000015777
	Propeptide	57 – 87	31	C peptide		PRO_0000015778
	Peptide	90 – 110	21	Insulin A chain		PRO_0000015779
Amino acid modifications
	Disulfide bond	31 ↔ 96		Interchain (between B and A chains)
	Disulfide bond	43 ↔ 109		Interchain (between B and A chains)
	Disulfide bond	95 ↔ 100

Sequences

	Sequence		Length	Mass (Da)
	P01321-1 [UniParc]. Last modified July 21, 1986. Version 1. Checksum: A574791864A4FB98	FASTA	110	12,190
	10 20 30 40 50 60 MALWMRLLPL LALLALWAPA PTRAFVNQHL CGSHLVEALY LVCGERGFFY TPKARREVED 70 80 90 100 110 LQVRDVELAG APGEGGLQPL ALEGALQKRG IVEQCCTSIC SLYQLENYCN

References

[1]	"Cloning and nucleotide sequence analysis of the dog insulin gene. Coded amino acid sequence of canine preproinsulin predicts an additional C-peptide fragment." Kwok S.C.M., Chan S.J., Steiner D.F. J. Biol. Chem. 258:2357-2363(1983) [PubMed: 6296142] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Species variation in the amino acid sequence of insulin." Smith L.F. Am. J. Med. 40:662-666(1966) [PubMed: 5949593] [Abstract] Cited for: PROTEIN SEQUENCE OF 25-54 AND 90-110.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	V00179 Genomic DNA. Translation: CAA23475.1.
PIR	IPDG. A92413.
RefSeq	NP_001123565.1.
UniGene	Cfa.18796
3D structure databases
SMR	P01321. Positions 25-110.
ModBase	Search...
Protein-protein interaction databases
STRING	P01321.
Genome annotation databases
Ensembl	ENSCAFT00000016041; ENSCAFP00000014836; ENSCAFG00000010092; Canis familiaris. [Genome view]
GeneID	483665.
KEGG	cfa:483665.
Organism-specific databases
CTD	483665.
Phylogenomic databases
eggNOG	maNOG20964.
HOVERGEN	HBG006137.
InParanoid	P01321.
OMA	PKARRDV.
OrthoDB	EOG9JQ6H6.
PhylomeDB	P01321.
Family and domain databases
InterPro	IPR004825. Ins/IGF/relaxin. IPR016179. Insulin-like. [Graphical view]
Gene3D	G3DSA:1.10.100.10. Ins/IGF/relaxin. 1 hit.
Pfam	PF00049. Insulin. 1 hit. [Graphical view]
PRINTS	PR00277. INSULINB.
SMART	SM00078. IlGF. 1 hit. [Graphical view]
SUPFAM	SSF56994. Insulin-like. 1 hit.
PROSITE	PS00262. INSULIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

INS_CANFA

Accession

Primary (citable) accession number: P01321

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	March 2, 2010
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)