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P01317 (INS_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 142. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Insulin

Cleaved into the following 2 chains:

  1. Insulin B chain
  2. Insulin A chain
Gene names
Name:INS
OrganismBos taurus (Bovine) [Reference proteome]
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos

Protein attributes

Sequence length105 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.

Subunit structure

Heterodimer of a B chain and an A chain linked by two disulfide bonds.

Subcellular location

Secreted.

Sequence similarities

Belongs to the insulin family.

Ontologies

Keywords
   Biological processCarbohydrate metabolism
Glucose metabolism
   Cellular componentSecreted
   DomainSignal
   Molecular functionHormone
   PTMCleavage on pair of basic residues
Disulfide bond
   Technical term3D-structure
Complete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processglucose metabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionidentical protein binding

Inferred from physical interaction PubMed 22001209PubMed 23416304. Source: IntAct

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

itself6EBI-3989070,EBI-3989070
Sama_0787A1S3N82EBI-3989070,EBI-7016414From a different organism.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2424 Ref.2 Ref.3 Ref.7
Peptide25 – 5430Insulin B chain Ref.1 Ref.3 Ref.7
PRO_0000015764
Propeptide57 – 8226C peptide Ref.4 Ref.5
PRO_0000015765
Peptide85 – 10521Insulin A chain Ref.1 Ref.6
PRO_0000015766

Amino acid modifications

Disulfide bond31 ↔ 91Interchain (between B and A chains) Ref.7
Disulfide bond43 ↔ 104Interchain (between B and A chains) Ref.7
Disulfide bond90 ↔ 95 Ref.2 Ref.7

Secondary structure

........... 105
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P01317 [UniParc].

Last modified August 1, 1992. Version 2.
Checksum: 75307CF78E61C06A

FASTA10511,393
        10         20         30         40         50         60 
MALWTRLRPL LALLALWPPP PARAFVNQHL CGSHLVEALY LVCGERGFFY TPKARREVEG 

        70         80         90        100 
PQVGALELAG GPGAGGLEGP PQKRGIVEQC CASVCSLYQL ENYCN 

« Hide

References

[1]"Cloning and nucleotide sequence analysis of complementary deoxyribonucleic acid for bovine preproinsulin."
D'Agostino J., Younes M.A., White J.W., Besch P.K., Field J.B., Frazier M.L.
Mol. Endocrinol. 1:327-331(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"The structure of bovine proinsulin."
Nolan C., Margoliash E., Peterson J.D., Steiner D.F.
J. Biol. Chem. 246:2780-2795(1971) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 25-105.
[3]"The amino-acid sequence in the phenylalanyl chain of insulin. 2. The investigation of peptides from enzymic hydrolysates."
Sanger F., Tuppy H.
Biochem. J. 49:481-490(1951) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 25-54.
[4]"Isolation and characterization of proinsulin C-peptide from bovine pancreas."
Steiner D.F., Cho S., Oyer P.E., Terris S., Peterson J.D., Rubenstein A.H.
J. Biol. Chem. 246:1365-1374(1971) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 57-82.
[5]"Bovine proinsulin: amino acid sequence of the C-peptide isolated from pancreas."
Salokangas A., Smyth D.G., Markussen J., Sundby F.
Eur. J. Biochem. 20:183-189(1971) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 57-82.
[6]"The amino-acid sequence in the glycyl chain of insulin. 2. The investigation of peptides from enzymic hydrolysates."
Sanger F., Thompson E.O.P.
Biochem. J. 53:366-374(1953) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 85-105.
[7]"The disulphide bonds of insulin."
Ryle A.P., Sanger F., Smith L.F., Kitai R.
Biochem. J. 60:541-556(1955) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 25-54 AND 85-105, DISULFIDE BONDS.
[8]"The structure of des-Phe b1 bovine insulin."
Smith G.D., Duax W.L., Dodson E.J., Dodson G.G., de Graaf R.A.G., Reynolds C.D.
Acta Crystallogr. B 38:3028-3032(1982)
Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF 26-54.
[9]"A model of insulin fibrils derived from the X-ray crystal structure of a monomeric insulin (despentapeptide insulin)."
Brange J., Dodson G.G., Edwards D.J., Holden P.H., Whittingham J.L.
Proteins 27:507-516(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.3 ANGSTROMS) OF 25-49.
+Additional computationally mapped references.

Web resources

Protein Spotlight

Protein of the 20th century - Issue 9 of April 2001

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M54979 mRNA. Translation: AAA30722.1.
PIRIPBO. A40909.
UniGeneBt.453.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1APHX-ray2.00A85-105[»]
B25-54[»]
1BPHX-ray2.00A85-105[»]
B25-54[»]
1CPHX-ray1.90A85-105[»]
B25-54[»]
1DPHX-ray1.90A85-105[»]
B25-54[»]
1HO0NMR-A25-54[»]
1PIDX-ray1.30A/C85-105[»]
B/D25-49[»]
2A3GX-ray2.25A/C85-105[»]
B/D25-54[»]
2BN1X-ray1.40A85-105[»]
B25-54[»]
2BN3X-ray1.40A85-105[»]
B25-54[»]
2INSX-ray2.50A/C85-105[»]
B/D26-54[»]
2ZP6X-ray2.56A/C85-105[»]
B/D25-54[»]
3W14X-ray4.40A/I85-105[»]
B/J25-54[»]
4BS3X-ray2.30A85-105[»]
B25-54[»]
4E7TX-ray1.40A/C85-105[»]
B/D25-54[»]
4E7UX-ray1.30A/C85-105[»]
B/D25-54[»]
4E7VX-ray1.801/3/5/A/C/E/G/I/K/M/O/Q/S/U/W/Y85-105[»]
2/4/6/B/D/F/H/J/L/N/P/R/T/V/X/Z25-54[»]
4I5YX-ray1.80A85-105[»]
B25-54[»]
4I5ZX-ray1.80A85-105[»]
B25-54[»]
4IDWX-ray2.70A/C85-105[»]
B/D25-54[»]
4IHNX-ray1.16A85-105[»]
B25-54[»]
4M4FX-ray1.90A/C85-105[»]
B/D25-54[»]
4M4HX-ray1.90A/C85-105[»]
B/D25-54[»]
4M4IX-ray1.90A/C85-105[»]
B/D25-54[»]
4M4JX-ray1.90A/C85-105[»]
B/D25-54[»]
4M4LX-ray1.45A/C85-105[»]
B/D25-54[»]
4M4MX-ray1.50A/C85-105[»]
B/D25-54[»]
ProteinModelPortalP01317.
SMRP01317. Positions 25-105.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-52901N.
IntActP01317. 5 interactions.

Protein family/group databases

Allergome2118. Bos d Insulin.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Phylogenomic databases

eggNOGNOG45999.
HOGENOMHOG000261669.
HOVERGENHBG006137.
InParanoidP01317.

Family and domain databases

Gene3D1.10.100.10. 1 hit.
InterProIPR004825. Insulin.
IPR016179. Insulin-like.
IPR022353. Insulin_CS.
IPR022352. Insulin_family.
[Graphical view]
PfamPF00049. Insulin. 1 hit.
[Graphical view]
PRINTSPR00277. INSULIN.
PR00276. INSULINFAMLY.
SMARTSM00078. IlGF. 1 hit.
[Graphical view]
SUPFAMSSF56994. SSF56994. 1 hit.
PROSITEPS00262. INSULIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP01317.
NextBio20804979.
PMAP-CutDBP01317.

Entry information

Entry nameINS_BOVIN
AccessionPrimary (citable) accession number: P01317
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: August 1, 1992
Last modified: April 16, 2014
This is version 142 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Protein Spotlight

Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries

PDB cross-references

Index of Protein Data Bank (PDB) cross-references