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P01317

- INS_BOVIN

UniProt

P01317 - INS_BOVIN

Protein

Insulin

Gene

INS

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.

    GO - Molecular functioni

    1. identical protein binding Source: IntAct
    2. protein binding Source: IntAct

    GO - Biological processi

    1. glucose metabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hormone

    Keywords - Biological processi

    Carbohydrate metabolism, Glucose metabolism

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Insulin
    Cleaved into the following 2 chains:
    Gene namesi
    Name:INS
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Unplaced

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    Pathology & Biotechi

    Protein family/group databases

    Allergomei2118. Bos d Insulin.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 24243 PublicationsAdd
    BLAST
    Peptidei25 – 5430Insulin B chain1 PublicationPRO_0000015764Add
    BLAST
    Propeptidei57 – 8226C peptidePRO_0000015765Add
    BLAST
    Peptidei85 – 10521Insulin A chain1 PublicationPRO_0000015766Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi31 ↔ 91Interchain (between B and A chains)1 Publication
    Disulfide bondi43 ↔ 104Interchain (between B and A chains)1 Publication
    Disulfide bondi90 ↔ 952 Publications

    Keywords - PTMi

    Cleavage on pair of basic residues, Disulfide bond

    Miscellaneous databases

    PMAP-CutDBP01317.

    Interactioni

    Subunit structurei

    Heterodimer of a B chain and an A chain linked by two disulfide bonds.

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    itself6EBI-3989070,EBI-3989070
    IGF1RP080694EBI-3989070,EBI-475981From a different organism.
    INSRP062135EBI-3989070,EBI-475899From a different organism.
    Sama_0787A1S3N82EBI-3989070,EBI-7016414From a different organism.

    Protein-protein interaction databases

    DIPiDIP-52901N.
    IntActiP01317. 5 interactions.

    Structurei

    Secondary structure

    1
    105
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi33 – 4311
    Helixi44 – 463
    Beta strandi48 – 503
    Helixi86 – 905
    Turni91 – 944
    Helixi97 – 1015

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1APHX-ray2.00A85-105[»]
    B25-54[»]
    1BPHX-ray2.00A85-105[»]
    B25-54[»]
    1CPHX-ray1.90A85-105[»]
    B25-54[»]
    1DPHX-ray1.90A85-105[»]
    B25-54[»]
    1HO0NMR-A25-54[»]
    1PIDX-ray1.30A/C85-105[»]
    B/D25-49[»]
    2A3GX-ray2.25A/C85-105[»]
    B/D25-54[»]
    2BN1X-ray1.40A85-105[»]
    B25-54[»]
    2BN3X-ray1.40A85-105[»]
    B25-54[»]
    2INSX-ray2.50A/C85-105[»]
    B/D26-54[»]
    2ZP6X-ray2.56A/C85-105[»]
    B/D25-54[»]
    3W14X-ray4.40A/I85-105[»]
    B/J25-54[»]
    4BS3X-ray2.30A85-105[»]
    B25-54[»]
    4E7TX-ray1.40A/C85-105[»]
    B/D25-54[»]
    4E7UX-ray1.30A/C85-105[»]
    B/D25-54[»]
    4E7VX-ray1.801/3/5/A/C/E/G/I/K/M/O/Q/S/U/W/Y85-105[»]
    2/4/6/B/D/F/H/J/L/N/P/R/T/V/X/Z25-54[»]
    4I5YX-ray1.80A85-105[»]
    B25-54[»]
    4I5ZX-ray1.80A85-105[»]
    B25-54[»]
    4IDWX-ray2.70A/C85-105[»]
    B/D25-54[»]
    4IHNX-ray1.16A85-105[»]
    B25-54[»]
    4M4FX-ray1.90A/C85-105[»]
    B/D25-54[»]
    4M4HX-ray1.90A/C85-105[»]
    B/D25-54[»]
    4M4IX-ray1.90A/C85-105[»]
    B/D25-54[»]
    4M4JX-ray1.90A/C85-105[»]
    B/D25-54[»]
    4M4LX-ray1.45A/C85-105[»]
    B/D25-54[»]
    4M4MX-ray1.50A/C85-105[»]
    B/D25-54[»]
    ProteinModelPortaliP01317.
    SMRiP01317. Positions 25-105.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP01317.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the insulin family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG45999.
    HOGENOMiHOG000261669.
    HOVERGENiHBG006137.
    InParanoidiP01317.

    Family and domain databases

    Gene3Di1.10.100.10. 1 hit.
    InterProiIPR004825. Insulin.
    IPR016179. Insulin-like.
    IPR022353. Insulin_CS.
    IPR022352. Insulin_family.
    [Graphical view]
    PfamiPF00049. Insulin. 1 hit.
    [Graphical view]
    PRINTSiPR00277. INSULIN.
    PR00276. INSULINFAMLY.
    SMARTiSM00078. IlGF. 1 hit.
    [Graphical view]
    SUPFAMiSSF56994. SSF56994. 1 hit.
    PROSITEiPS00262. INSULIN. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P01317-1 [UniParc]FASTAAdd to Basket

    « Hide

    MALWTRLRPL LALLALWPPP PARAFVNQHL CGSHLVEALY LVCGERGFFY    50
    TPKARREVEG PQVGALELAG GPGAGGLEGP PQKRGIVEQC CASVCSLYQL 100
    ENYCN 105
    Length:105
    Mass (Da):11,393
    Last modified:August 1, 1992 - v2
    Checksum:i75307CF78E61C06A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M54979 mRNA. Translation: AAA30722.1.
    PIRiA40909. IPBO.
    UniGeneiBt.453.

    Cross-referencesi

    Web resourcesi

    Protein Spotlight

    Protein of the 20th century - Issue 9 of April 2001

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M54979 mRNA. Translation: AAA30722.1 .
    PIRi A40909. IPBO.
    UniGenei Bt.453.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1APH X-ray 2.00 A 85-105 [» ]
    B 25-54 [» ]
    1BPH X-ray 2.00 A 85-105 [» ]
    B 25-54 [» ]
    1CPH X-ray 1.90 A 85-105 [» ]
    B 25-54 [» ]
    1DPH X-ray 1.90 A 85-105 [» ]
    B 25-54 [» ]
    1HO0 NMR - A 25-54 [» ]
    1PID X-ray 1.30 A/C 85-105 [» ]
    B/D 25-49 [» ]
    2A3G X-ray 2.25 A/C 85-105 [» ]
    B/D 25-54 [» ]
    2BN1 X-ray 1.40 A 85-105 [» ]
    B 25-54 [» ]
    2BN3 X-ray 1.40 A 85-105 [» ]
    B 25-54 [» ]
    2INS X-ray 2.50 A/C 85-105 [» ]
    B/D 26-54 [» ]
    2ZP6 X-ray 2.56 A/C 85-105 [» ]
    B/D 25-54 [» ]
    3W14 X-ray 4.40 A/I 85-105 [» ]
    B/J 25-54 [» ]
    4BS3 X-ray 2.30 A 85-105 [» ]
    B 25-54 [» ]
    4E7T X-ray 1.40 A/C 85-105 [» ]
    B/D 25-54 [» ]
    4E7U X-ray 1.30 A/C 85-105 [» ]
    B/D 25-54 [» ]
    4E7V X-ray 1.80 1/3/5/A/C/E/G/I/K/M/O/Q/S/U/W/Y 85-105 [» ]
    2/4/6/B/D/F/H/J/L/N/P/R/T/V/X/Z 25-54 [» ]
    4I5Y X-ray 1.80 A 85-105 [» ]
    B 25-54 [» ]
    4I5Z X-ray 1.80 A 85-105 [» ]
    B 25-54 [» ]
    4IDW X-ray 2.70 A/C 85-105 [» ]
    B/D 25-54 [» ]
    4IHN X-ray 1.16 A 85-105 [» ]
    B 25-54 [» ]
    4M4F X-ray 1.90 A/C 85-105 [» ]
    B/D 25-54 [» ]
    4M4H X-ray 1.90 A/C 85-105 [» ]
    B/D 25-54 [» ]
    4M4I X-ray 1.90 A/C 85-105 [» ]
    B/D 25-54 [» ]
    4M4J X-ray 1.90 A/C 85-105 [» ]
    B/D 25-54 [» ]
    4M4L X-ray 1.45 A/C 85-105 [» ]
    B/D 25-54 [» ]
    4M4M X-ray 1.50 A/C 85-105 [» ]
    B/D 25-54 [» ]
    ProteinModelPortali P01317.
    SMRi P01317. Positions 25-105.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-52901N.
    IntActi P01317. 5 interactions.

    Protein family/group databases

    Allergomei 2118. Bos d Insulin.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Phylogenomic databases

    eggNOGi NOG45999.
    HOGENOMi HOG000261669.
    HOVERGENi HBG006137.
    InParanoidi P01317.

    Miscellaneous databases

    EvolutionaryTracei P01317.
    NextBioi 20804979.
    PMAP-CutDB P01317.

    Family and domain databases

    Gene3Di 1.10.100.10. 1 hit.
    InterProi IPR004825. Insulin.
    IPR016179. Insulin-like.
    IPR022353. Insulin_CS.
    IPR022352. Insulin_family.
    [Graphical view ]
    Pfami PF00049. Insulin. 1 hit.
    [Graphical view ]
    PRINTSi PR00277. INSULIN.
    PR00276. INSULINFAMLY.
    SMARTi SM00078. IlGF. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56994. SSF56994. 1 hit.
    PROSITEi PS00262. INSULIN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and nucleotide sequence analysis of complementary deoxyribonucleic acid for bovine preproinsulin."
      D'Agostino J., Younes M.A., White J.W., Besch P.K., Field J.B., Frazier M.L.
      Mol. Endocrinol. 1:327-331(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "The structure of bovine proinsulin."
      Nolan C., Margoliash E., Peterson J.D., Steiner D.F.
      J. Biol. Chem. 246:2780-2795(1971) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 25-105.
    3. "The amino-acid sequence in the phenylalanyl chain of insulin. 2. The investigation of peptides from enzymic hydrolysates."
      Sanger F., Tuppy H.
      Biochem. J. 49:481-490(1951) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 25-54.
    4. "Isolation and characterization of proinsulin C-peptide from bovine pancreas."
      Steiner D.F., Cho S., Oyer P.E., Terris S., Peterson J.D., Rubenstein A.H.
      J. Biol. Chem. 246:1365-1374(1971) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 57-82.
    5. "Bovine proinsulin: amino acid sequence of the C-peptide isolated from pancreas."
      Salokangas A., Smyth D.G., Markussen J., Sundby F.
      Eur. J. Biochem. 20:183-189(1971) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 57-82.
    6. "The amino-acid sequence in the glycyl chain of insulin. 2. The investigation of peptides from enzymic hydrolysates."
      Sanger F., Thompson E.O.P.
      Biochem. J. 53:366-374(1953) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 85-105.
    7. "The disulphide bonds of insulin."
      Ryle A.P., Sanger F., Smith L.F., Kitai R.
      Biochem. J. 60:541-556(1955) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 25-54 AND 85-105, DISULFIDE BONDS.
    8. "The structure of des-Phe b1 bovine insulin."
      Smith G.D., Duax W.L., Dodson E.J., Dodson G.G., de Graaf R.A.G., Reynolds C.D.
      Acta Crystallogr. B 38:3028-3032(1982)
      Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF 26-54.
    9. "A model of insulin fibrils derived from the X-ray crystal structure of a monomeric insulin (despentapeptide insulin)."
      Brange J., Dodson G.G., Edwards D.J., Holden P.H., Whittingham J.L.
      Proteins 27:507-516(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.3 ANGSTROMS) OF 25-49.

    Entry informationi

    Entry nameiINS_BOVIN
    AccessioniPrimary (citable) accession number: P01317
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: August 1, 1992
    Last modified: October 1, 2014
    This is version 146 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. Protein Spotlight
      Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3