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P01315

- INS_PIG

UniProt

P01315 - INS_PIG

Protein

Insulin

Gene

INS

Organism
Sus scrofa (Pig)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.

    GO - Molecular functioni

    1. identical protein binding Source: IntAct

    GO - Biological processi

    1. glucose metabolic process Source: UniProtKB-KW
    2. positive regulation of cell migration Source: BHF-UCL
    3. positive regulation of DNA replication Source: BHF-UCL
    4. positive regulation of insulin receptor signaling pathway Source: BHF-UCL
    5. positive regulation of peptidyl-tyrosine phosphorylation Source: BHF-UCL
    6. positive regulation of protein autophosphorylation Source: BHF-UCL

    Keywords - Molecular functioni

    Hormone

    Keywords - Biological processi

    Carbohydrate metabolism, Glucose metabolism

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Insulin
    Cleaved into the following 2 chains:
    Gene namesi
    Name:INS
    OrganismiSus scrofa (Pig)
    Taxonomic identifieri9823 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
    ProteomesiUP000008227: Unplaced

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    Pathology & Biotechi

    Protein family/group databases

    Allergomei2122. Sus s Insulin.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 24241 PublicationAdd
    BLAST
    Peptidei25 – 5430Insulin B chainPRO_0000015879Add
    BLAST
    Propeptidei57 – 8529C peptidePRO_0000015880Add
    BLAST
    Peptidei88 – 10821Insulin A chainPRO_0000015881Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi31 ↔ 94Interchain (between B and A chains)
    Disulfide bondi43 ↔ 107Interchain (between B and A chains)
    Disulfide bondi93 ↔ 98

    Keywords - PTMi

    Cleavage on pair of basic residues, Disulfide bond

    Interactioni

    Subunit structurei

    Heterodimer of a B chain and an A chain linked by two disulfide bonds.

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    itself2EBI-8437944,EBI-8437944

    Protein-protein interaction databases

    IntActiP01315. 1 interaction.
    MINTiMINT-1505666.

    Structurei

    Secondary structure

    1
    108
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi33 – 4311
    Helixi44 – 463
    Beta strandi48 – 503
    Helixi89 – 957
    Helixi100 – 1034
    Helixi104 – 1063

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1B17X-ray1.70A88-108[»]
    B25-54[»]
    1B18X-ray1.80A88-108[»]
    B25-54[»]
    1B19X-ray1.80A88-108[»]
    B25-54[»]
    1B2AX-ray1.70A88-108[»]
    B25-54[»]
    1B2BX-ray1.80A88-108[»]
    B25-54[»]
    1B2CX-ray1.80A88-108[»]
    B25-54[»]
    1B2DX-ray1.70A88-108[»]
    B25-54[»]
    1B2EX-ray1.90A88-108[»]
    B25-54[»]
    1B2FX-ray1.90A88-108[»]
    B25-54[»]
    1B2GX-ray1.80A88-108[»]
    B25-54[»]
    1DEIX-ray1.60A/C88-108[»]
    B/D25-47[»]
    1IZAX-ray2.50A/C88-108[»]
    B/D25-53[»]
    1IZBX-ray2.00A/C88-108[»]
    B/D25-53[»]
    1M5AX-ray1.20A/C88-108[»]
    B/D25-54[»]
    1MPJX-ray2.30A/C88-108[»]
    B/D25-54[»]
    1SDBX-ray1.65A88-108[»]
    B27-49[»]
    1WAVX-ray2.50A/C/E/G/I/K88-108[»]
    B/D/F/H/J/L25-54[»]
    1ZEIX-ray1.90A/B/C/D/E/F25-54[»]
    A/B/C/D/E/F88-108[»]
    1ZNIX-ray1.50A/C88-108[»]
    B/D25-54[»]
    2EFAneutron diffraction2.70A88-108[»]
    B25-54[»]
    2G4MX-ray1.80A88-108[»]
    B25-54[»]
    2TCIX-ray1.80A/C88-108[»]
    B/D25-54[»]
    2ZPPneutron diffraction2.50A88-108[»]
    B25-54[»]
    3FHPneutron diffraction2.00A/C88-108[»]
    B/D25-54[»]
    3GKYX-ray1.80A/C88-108[»]
    B/D25-54[»]
    3INSX-ray1.50A/C88-108[»]
    B/D25-54[»]
    3MTHX-ray1.90A/C88-108[»]
    B/D25-54[»]
    3RTOX-ray1.80A/C88-108[»]
    B/D25-54[»]
    3T2AX-ray2.10A88-108[»]
    B25-54[»]
    4A7EX-ray1.86A88-108[»]
    B25-54[»]
    4INSX-ray1.50A/C88-108[»]
    B/D25-54[»]
    6INSX-ray2.00E/F25-108[»]
    7INSX-ray2.00A/C/E88-108[»]
    B/D/F25-54[»]
    9INSX-ray1.70A88-108[»]
    B25-54[»]
    ProteinModelPortaliP01315.
    SMRiP01315. Positions 25-108.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP01315.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the insulin family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    HOVERGENiHBG006137.
    KOiK04526.

    Family and domain databases

    Gene3Di1.10.100.10. 1 hit.
    InterProiIPR004825. Insulin.
    IPR016179. Insulin-like.
    IPR022353. Insulin_CS.
    IPR022352. Insulin_family.
    [Graphical view]
    PfamiPF00049. Insulin. 1 hit.
    [Graphical view]
    PRINTSiPR00277. INSULIN.
    PR00276. INSULINFAMLY.
    SMARTiSM00078. IlGF. 1 hit.
    [Graphical view]
    SUPFAMiSSF56994. SSF56994. 1 hit.
    PROSITEiPS00262. INSULIN. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P01315-1 [UniParc]FASTAAdd to Basket

    « Hide

    MALWTRLLPL LALLALWAPA PAQAFVNQHL CGSHLVEALY LVCGERGFFY    50
    TPKARREAEN PQAGAVELGG GLGGLQALAL EGPPQKRGIV EQCCTSICSL 100
    YQLENYCN 108
    Length:108
    Mass (Da):11,672
    Last modified:December 1, 2000 - v2
    Checksum:iCB4491B429858EBE
    GO

    Sequence cautioni

    The sequence AAC77920.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF064555 mRNA. Translation: AAC77920.1. Different initiation.
    AY044828 Genomic DNA. Translation: AAL69550.1.
    AY242098 Genomic DNA. Translation: AAQ00952.1.
    AY242099 Genomic DNA. Translation: AAQ00954.1.
    AY242100 Genomic DNA. Translation: AAQ00957.1.
    AY242101 Genomic DNA. Translation: AAQ00960.1.
    AY242102 Genomic DNA. Translation: AAQ00963.1.
    AY242103 Genomic DNA. Translation: AAQ00966.1.
    AY242104 Genomic DNA. Translation: AAQ00969.1.
    AY242105 Genomic DNA. Translation: AAQ00972.1.
    AY242106 Genomic DNA. Translation: AAQ00975.1.
    AY242107 Genomic DNA. Translation: AAQ00978.1.
    AY242108 Genomic DNA. Translation: AAQ00981.1.
    AY242109 Genomic DNA. Translation: AAQ00983.1.
    AY242110 Genomic DNA. Translation: AAQ00985.1.
    AY242111 Genomic DNA. Translation: AAQ00987.1.
    AY242112 Genomic DNA. Translation: AAQ00990.1.
    PIRiA01583. IPPG.
    RefSeqiNP_001103242.1. NM_001109772.1.
    UniGeneiSsc.583.

    Genome annotation databases

    GeneIDi397415.
    KEGGissc:397415.

    Cross-referencesi

    Web resourcesi

    Protein Spotlight

    Protein of the 20th century - Issue 9 of April 2001

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF064555 mRNA. Translation: AAC77920.1 . Different initiation.
    AY044828 Genomic DNA. Translation: AAL69550.1 .
    AY242098 Genomic DNA. Translation: AAQ00952.1 .
    AY242099 Genomic DNA. Translation: AAQ00954.1 .
    AY242100 Genomic DNA. Translation: AAQ00957.1 .
    AY242101 Genomic DNA. Translation: AAQ00960.1 .
    AY242102 Genomic DNA. Translation: AAQ00963.1 .
    AY242103 Genomic DNA. Translation: AAQ00966.1 .
    AY242104 Genomic DNA. Translation: AAQ00969.1 .
    AY242105 Genomic DNA. Translation: AAQ00972.1 .
    AY242106 Genomic DNA. Translation: AAQ00975.1 .
    AY242107 Genomic DNA. Translation: AAQ00978.1 .
    AY242108 Genomic DNA. Translation: AAQ00981.1 .
    AY242109 Genomic DNA. Translation: AAQ00983.1 .
    AY242110 Genomic DNA. Translation: AAQ00985.1 .
    AY242111 Genomic DNA. Translation: AAQ00987.1 .
    AY242112 Genomic DNA. Translation: AAQ00990.1 .
    PIRi A01583. IPPG.
    RefSeqi NP_001103242.1. NM_001109772.1.
    UniGenei Ssc.583.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1B17 X-ray 1.70 A 88-108 [» ]
    B 25-54 [» ]
    1B18 X-ray 1.80 A 88-108 [» ]
    B 25-54 [» ]
    1B19 X-ray 1.80 A 88-108 [» ]
    B 25-54 [» ]
    1B2A X-ray 1.70 A 88-108 [» ]
    B 25-54 [» ]
    1B2B X-ray 1.80 A 88-108 [» ]
    B 25-54 [» ]
    1B2C X-ray 1.80 A 88-108 [» ]
    B 25-54 [» ]
    1B2D X-ray 1.70 A 88-108 [» ]
    B 25-54 [» ]
    1B2E X-ray 1.90 A 88-108 [» ]
    B 25-54 [» ]
    1B2F X-ray 1.90 A 88-108 [» ]
    B 25-54 [» ]
    1B2G X-ray 1.80 A 88-108 [» ]
    B 25-54 [» ]
    1DEI X-ray 1.60 A/C 88-108 [» ]
    B/D 25-47 [» ]
    1IZA X-ray 2.50 A/C 88-108 [» ]
    B/D 25-53 [» ]
    1IZB X-ray 2.00 A/C 88-108 [» ]
    B/D 25-53 [» ]
    1M5A X-ray 1.20 A/C 88-108 [» ]
    B/D 25-54 [» ]
    1MPJ X-ray 2.30 A/C 88-108 [» ]
    B/D 25-54 [» ]
    1SDB X-ray 1.65 A 88-108 [» ]
    B 27-49 [» ]
    1WAV X-ray 2.50 A/C/E/G/I/K 88-108 [» ]
    B/D/F/H/J/L 25-54 [» ]
    1ZEI X-ray 1.90 A/B/C/D/E/F 25-54 [» ]
    A/B/C/D/E/F 88-108 [» ]
    1ZNI X-ray 1.50 A/C 88-108 [» ]
    B/D 25-54 [» ]
    2EFA neutron diffraction 2.70 A 88-108 [» ]
    B 25-54 [» ]
    2G4M X-ray 1.80 A 88-108 [» ]
    B 25-54 [» ]
    2TCI X-ray 1.80 A/C 88-108 [» ]
    B/D 25-54 [» ]
    2ZPP neutron diffraction 2.50 A 88-108 [» ]
    B 25-54 [» ]
    3FHP neutron diffraction 2.00 A/C 88-108 [» ]
    B/D 25-54 [» ]
    3GKY X-ray 1.80 A/C 88-108 [» ]
    B/D 25-54 [» ]
    3INS X-ray 1.50 A/C 88-108 [» ]
    B/D 25-54 [» ]
    3MTH X-ray 1.90 A/C 88-108 [» ]
    B/D 25-54 [» ]
    3RTO X-ray 1.80 A/C 88-108 [» ]
    B/D 25-54 [» ]
    3T2A X-ray 2.10 A 88-108 [» ]
    B 25-54 [» ]
    4A7E X-ray 1.86 A 88-108 [» ]
    B 25-54 [» ]
    4INS X-ray 1.50 A/C 88-108 [» ]
    B/D 25-54 [» ]
    6INS X-ray 2.00 E/F 25-108 [» ]
    7INS X-ray 2.00 A/C/E 88-108 [» ]
    B/D/F 25-54 [» ]
    9INS X-ray 1.70 A 88-108 [» ]
    B 25-54 [» ]
    ProteinModelPortali P01315.
    SMRi P01315. Positions 25-108.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P01315. 1 interaction.
    MINTi MINT-1505666.

    Protein family/group databases

    Allergomei 2122. Sus s Insulin.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 397415.
    KEGGi ssc:397415.

    Organism-specific databases

    CTDi 3630.

    Phylogenomic databases

    HOVERGENi HBG006137.
    KOi K04526.

    Miscellaneous databases

    EvolutionaryTracei P01315.

    Family and domain databases

    Gene3Di 1.10.100.10. 1 hit.
    InterProi IPR004825. Insulin.
    IPR016179. Insulin-like.
    IPR022353. Insulin_CS.
    IPR022352. Insulin_family.
    [Graphical view ]
    Pfami PF00049. Insulin. 1 hit.
    [Graphical view ]
    PRINTSi PR00277. INSULIN.
    PR00276. INSULINFAMLY.
    SMARTi SM00078. IlGF. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56994. SSF56994. 1 hit.
    PROSITEi PS00262. INSULIN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete porcine preproinsulin cDNA sequence."
      Han X.G., Tuch B.E.
      Submitted (MAY-1998) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE.
    2. "Comparative sequence analysis of the INS-IGF2-H19 gene cluster in pigs."
      Amarger V., Nguyen M., Van Laere A.-S., Braunschweig M., Nezer C., Georges M., Andersson L.
      Mamm. Genome 13:388-398(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: Large white.
    3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: European wild boar, Hampshire, Japanese wild boar, Landrace, Large white, Meishan and Pietrain.
    4. "Porcine proinsulin: characterization and amino acid sequence."
      Chance R.E., Ellis R.M., Bromer W.W.
      Science 161:165-167(1968) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 25-108.
    5. Chance R.E.
      Submitted (JUL-1970) to the PIR data bank
      Cited for: SEQUENCE REVISION TO 59.
    6. "Insulin. The structure in the crystal and its reflection in chemistry and biology."
      Blundell T.L., Dodson G.G., Hodgkin D., Mercola D.
      Adv. Protein Chem. 26:279-402(1972)
      Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS).
    7. "Experience with fast Fourier least squares in the refinement of the crystal structure of rhombohedral 2-zinc insulin at 1.5-A resolution."
      Isaacs N.W., Agarwal R.C.
      Acta Crystallogr. A 34:782-791(1978)
      Cited for: X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS).
    8. Cited for: X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS).
    9. "Structure of porcine insulin cocrystallized with clupeine Z."
      Balschmidt P., Hansen F.B., Dodson E., Dodson G., Korber F.
      Acta Crystallogr. B 47:975-986(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).
    10. Cited for: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS).
    11. "Structure of monomeric porcine DesB1-B2 despentapeptide (B26-B30) insulin at 1.65-A resolution."
      Diao J.-S., Wan Z.-L., Chang W.-R., Liang D.-C.
      Acta Crystallogr. D 53:507-512(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.65 ANGSTROMS).

    Entry informationi

    Entry nameiINS_PIG
    AccessioniPrimary (citable) accession number: P01315
    Secondary accession number(s): Q9TSJ5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: December 1, 2000
    Last modified: October 1, 2014
    This is version 137 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. Protein Spotlight
      Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3