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P01315 (INS_PIG) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 136. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Insulin

Cleaved into the following 2 chains:

  1. Insulin B chain
  2. Insulin A chain
Gene names
Name:INS
OrganismSus scrofa (Pig) [Reference proteome]
Taxonomic identifier9823 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus

Protein attributes

Sequence length108 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.

Subunit structure

Heterodimer of a B chain and an A chain linked by two disulfide bonds.

Subcellular location

Secreted.

Sequence similarities

Belongs to the insulin family.

Sequence caution

The sequence AAC77920.1 differs from that shown. Reason: Erroneous initiation.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

itself2EBI-8437944,EBI-8437944

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2424 Ref.4
Peptide25 – 5430Insulin B chain
PRO_0000015879
Propeptide57 – 8529C peptide
PRO_0000015880
Peptide88 – 10821Insulin A chain
PRO_0000015881

Amino acid modifications

Disulfide bond31 ↔ 94Interchain (between B and A chains)
Disulfide bond43 ↔ 107Interchain (between B and A chains)
Disulfide bond93 ↔ 98

Secondary structure

........... 108
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P01315 [UniParc].

Last modified December 1, 2000. Version 2.
Checksum: CB4491B429858EBE

FASTA10811,672
        10         20         30         40         50         60 
MALWTRLLPL LALLALWAPA PAQAFVNQHL CGSHLVEALY LVCGERGFFY TPKARREAEN 

        70         80         90        100 
PQAGAVELGG GLGGLQALAL EGPPQKRGIV EQCCTSICSL YQLENYCN 

« Hide

References

[1]"Complete porcine preproinsulin cDNA sequence."
Han X.G., Tuch B.E.
Submitted (MAY-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE.
[2]"Comparative sequence analysis of the INS-IGF2-H19 gene cluster in pigs."
Amarger V., Nguyen M., Van Laere A.-S., Braunschweig M., Nezer C., Georges M., Andersson L.
Mamm. Genome 13:388-398(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: Large white.
[3]"A regulatory mutation in IGF2 causes a major QTL effect on muscle growth in the pig."
Van Laere A.-S., Nguyen M., Braunschweig M., Nezer C., Collette C., Moreau L., Archibald A.L., Haley C., Buys N., Tally M., Andersson G., Georges M., Andersson L.
Nature 425:832-836(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: European wild boar, Hampshire, Japanese wild boar, Landrace, Large white, Meishan and Pietrain.
[4]"Porcine proinsulin: characterization and amino acid sequence."
Chance R.E., Ellis R.M., Bromer W.W.
Science 161:165-167(1968) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 25-108.
[5]Chance R.E.
Submitted (JUL-1970) to the PIR data bank
Cited for: SEQUENCE REVISION TO 59.
[6]"Insulin. The structure in the crystal and its reflection in chemistry and biology."
Blundell T.L., Dodson G.G., Hodgkin D., Mercola D.
Adv. Protein Chem. 26:279-402(1972)
Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS).
[7]"Experience with fast Fourier least squares in the refinement of the crystal structure of rhombohedral 2-zinc insulin at 1.5-A resolution."
Isaacs N.W., Agarwal R.C.
Acta Crystallogr. A 34:782-791(1978)
Cited for: X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS).
[8]"The structure of 2Zn pig insulin crystals at 1.5-A resolution."
Baker E.N., Blundell T.L., Cutfield J.F., Cutfield S.M., Dodson E.J., Dodson G.G., Crowfoot Hodgkin D.M., Hubbard R.E., Isaacs N.W., Reynolds C.D., Sakabe K., Sakabe N., Vijayan N.M.
Philos. Trans. R. Soc. Lond., B, Biol. Sci. 319:369-456(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS).
[9]"Structure of porcine insulin cocrystallized with clupeine Z."
Balschmidt P., Hansen F.B., Dodson E., Dodson G., Korber F.
Acta Crystallogr. B 47:975-986(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).
[10]"Structure of the pig insulin dimer in the cubic crystal."
Badger J., Harris M.R., Reynolds C.D., Evans A.C., Dodson E.J., Dodson G.G., North A.C.T.
Acta Crystallogr. B 47:127-136(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS).
[11]"Structure of monomeric porcine DesB1-B2 despentapeptide (B26-B30) insulin at 1.65-A resolution."
Diao J.-S., Wan Z.-L., Chang W.-R., Liang D.-C.
Acta Crystallogr. D 53:507-512(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.65 ANGSTROMS).
+Additional computationally mapped references.

Web resources

Protein Spotlight

Protein of the 20th century - Issue 9 of April 2001

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF064555 mRNA. Translation: AAC77920.1. Different initiation.
AY044828 Genomic DNA. Translation: AAL69550.1.
AY242098 Genomic DNA. Translation: AAQ00952.1.
AY242099 Genomic DNA. Translation: AAQ00954.1.
AY242100 Genomic DNA. Translation: AAQ00957.1.
AY242101 Genomic DNA. Translation: AAQ00960.1.
AY242102 Genomic DNA. Translation: AAQ00963.1.
AY242103 Genomic DNA. Translation: AAQ00966.1.
AY242104 Genomic DNA. Translation: AAQ00969.1.
AY242105 Genomic DNA. Translation: AAQ00972.1.
AY242106 Genomic DNA. Translation: AAQ00975.1.
AY242107 Genomic DNA. Translation: AAQ00978.1.
AY242108 Genomic DNA. Translation: AAQ00981.1.
AY242109 Genomic DNA. Translation: AAQ00983.1.
AY242110 Genomic DNA. Translation: AAQ00985.1.
AY242111 Genomic DNA. Translation: AAQ00987.1.
AY242112 Genomic DNA. Translation: AAQ00990.1.
PIRIPPG. A01583.
RefSeqNP_001103242.1. NM_001109772.1.
UniGeneSsc.583.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1B17X-ray1.70A88-108[»]
B25-54[»]
1B18X-ray1.80A88-108[»]
B25-54[»]
1B19X-ray1.80A88-108[»]
B25-54[»]
1B2AX-ray1.70A88-108[»]
B25-54[»]
1B2BX-ray1.80A88-108[»]
B25-54[»]
1B2CX-ray1.80A88-108[»]
B25-54[»]
1B2DX-ray1.70A88-108[»]
B25-54[»]
1B2EX-ray1.90A88-108[»]
B25-54[»]
1B2FX-ray1.90A88-108[»]
B25-54[»]
1B2GX-ray1.80A88-108[»]
B25-54[»]
1DEIX-ray1.60A/C88-108[»]
B/D25-47[»]
1IZAX-ray2.50A/C88-108[»]
B/D25-53[»]
1IZBX-ray2.00A/C88-108[»]
B/D25-53[»]
1M5AX-ray1.20A/C88-108[»]
B/D25-54[»]
1MPJX-ray2.30A/C88-108[»]
B/D25-54[»]
1SDBX-ray1.65A88-108[»]
B27-49[»]
1WAVX-ray2.50A/C/E/G/I/K88-108[»]
B/D/F/H/J/L25-54[»]
1ZEIX-ray1.90A/B/C/D/E/F25-54[»]
A/B/C/D/E/F88-108[»]
1ZNIX-ray1.50A/C88-108[»]
B/D25-54[»]
2EFAneutron diffraction2.70A88-108[»]
B25-54[»]
2G4MX-ray1.80A88-108[»]
B25-54[»]
2TCIX-ray1.80A/C88-108[»]
B/D25-54[»]
2ZPPneutron diffraction2.50A88-108[»]
B25-54[»]
3FHPneutron diffraction2.00A/C88-108[»]
B/D25-54[»]
3GKYX-ray1.80A/C88-108[»]
B/D25-54[»]
3INSX-ray1.50A/C88-108[»]
B/D25-54[»]
3MTHX-ray1.90A/C88-108[»]
B/D25-54[»]
3RTOX-ray1.80A/C88-108[»]
B/D25-54[»]
3T2AX-ray2.10A88-108[»]
B25-54[»]
4A7EX-ray1.86A88-108[»]
B25-54[»]
4INSX-ray1.50A/C88-108[»]
B/D25-54[»]
6INSX-ray2.00E/F25-108[»]
7INSX-ray2.00A/C/E88-108[»]
B/D/F25-54[»]
9INSX-ray1.70A88-108[»]
B25-54[»]
ProteinModelPortalP01315.
SMRP01315. Positions 25-108.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

MINTMINT-1505666.

Protein family/group databases

Allergome2122. Sus s Insulin.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID397415.
KEGGssc:397415.

Organism-specific databases

CTD3630.

Phylogenomic databases

HOVERGENHBG006137.
KOK04526.

Family and domain databases

Gene3D1.10.100.10. 1 hit.
InterProIPR004825. Insulin.
IPR016179. Insulin-like.
IPR022353. Insulin_CS.
IPR022352. Insulin_family.
[Graphical view]
PfamPF00049. Insulin. 1 hit.
[Graphical view]
PRINTSPR00277. INSULIN.
PR00276. INSULINFAMLY.
SMARTSM00078. IlGF. 1 hit.
[Graphical view]
SUPFAMSSF56994. SSF56994. 1 hit.
PROSITEPS00262. INSULIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP01315.

Entry information

Entry nameINS_PIG
AccessionPrimary (citable) accession number: P01315
Secondary accession number(s): Q9TSJ5
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: December 1, 2000
Last modified: July 9, 2014
This is version 136 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Protein Spotlight

Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries

PDB cross-references

Index of Protein Data Bank (PDB) cross-references