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Protein

Protachykinin-1

Gene

TAC1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Tachykinins are active peptides which excite neurons, evoke behavioral responses, are potent vasodilators and secretagogues, and contract (directly or indirectly) many smooth muscles.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Neuropeptide, Neurotransmitter

Enzyme and pathway databases

ReactomeiR-BTA-380095. Tachykinin receptors bind tachykinins.
R-BTA-416476. G alpha (q) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
Protachykinin-1
Alternative name(s):
PPT
Cleaved into the following 5 chains:
Neurokinin A
Short name:
NKA
Alternative name(s):
Neuromedin L
Substance K
Neuropeptide K
Short name:
NPK
Gene namesi
Name:TAC1
Synonyms:NKA, NKNA, TAC2
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 4

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919Sequence analysisAdd
BLAST
Propeptidei20 – 5637Sequence analysisPRO_0000033522Add
BLAST
Peptidei58 – 6811Substance PPRO_0000033523Add
BLAST
Peptidei72 – 10736Neuropeptide KPRO_0000033524Add
BLAST
Peptidei72 – 732Neuropeptide gamma, 1st partPRO_0000033525
Peptidei89 – 10719Neuropeptide gamma, 2nd partPRO_0000033526Add
BLAST
Peptidei98 – 10710Neurokinin APRO_0000033527
Peptidei111 – 12616C-terminal-flanking peptidePRO_0000033528Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei68 – 681Methionine amide1 Publication
Modified residuei107 – 1071Methionine amide

Post-translational modificationi

The substance P form is cleaved at Pro-59 by the prolyl endopeptidase FAP (seprase) activity (in vitro).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei59 – 602Cleavage; by FAPBy similarity

Keywords - PTMi

Amidation, Cleavage on pair of basic residues

Proteomic databases

PaxDbiP01289.
PRIDEiP01289.

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000020419.

Structurei

3D structure databases

ProteinModelPortaliP01289.
SMRiP01289. Positions 72-107.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the tachykinin family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IWZZ. Eukaryota.
ENOG4112BW1. LUCA.
GeneTreeiENSGT00390000002457.
HOGENOMiHOG000082514.
HOVERGENiHBG000127.
InParanoidiP01289.
KOiK05239.
OMAiEMGANDD.
OrthoDBiEOG7C2R3N.
TreeFamiTF333405.

Family and domain databases

InterProiIPR013055. Tachy_Neuro_lke_CS.
IPR008215. Tachykinin_dom.
IPR008216. Tachykinin_fam.
[Graphical view]
PANTHERiPTHR11250. PTHR11250. 1 hit.
PRINTSiPR01829. PROTACHYKNIN.
SMARTiSM00203. TK. 2 hits.
[Graphical view]
PROSITEiPS00267. TACHYKININ. 2 hits.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Beta (identifier: P01289-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKILVAVAVI FFISTQLSAE EIGANDDFNY WSDWSDSDQI KEEMPEPFEH
60 70 80 90 100
LLQRIARRPK PQQFFGLMGK RDADSSIEKQ VALLKALYGH GQLSHKRHKT
110 120 130
DSFVGLMGKR ALNSVAYERS VMQDYERRRK
Length:130
Mass (Da):15,076
Last modified:July 21, 1986 - v2
Checksum:iCE2A28572305DEB7
GO
Isoform Alpha (identifier: P01289-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     97-114: Missing.
     115-115: V → M

Show »
Length:112
Mass (Da):13,109
Checksum:i8E1976CB5D065953
GO
Isoform Gamma (identifier: P01289-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     74-88: Missing.

Show »
Length:115
Mass (Da):13,416
Checksum:iB08A0FF9D53D3B53
GO
Isoform Delta (identifier: P01289-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     74-88: Missing.
     97-114: Missing.
     115-115: V → M

Show »
Length:97
Mass (Da):11,449
Checksum:i301332BA48D40969
GO

Sequence cautioni

The sequence CAA24943.1 differs from that shown.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti121 – 1211V → A in AAA30724 (PubMed:1708336).Curated
Sequence conflicti121 – 1211V → A in AAA30725 (PubMed:1708336).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei74 – 8815Missing in isoform Gamma and isoform Delta. 2 PublicationsVSP_006372Add
BLAST
Alternative sequencei97 – 11418Missing in isoform Alpha and isoform Delta. 1 PublicationVSP_006373Add
BLAST
Alternative sequencei115 – 1151V → M in isoform Alpha and isoform Delta. 1 PublicationVSP_006374

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X00075 mRNA. Translation: CAA24939.1.
X00075 mRNA. Translation: CAA24940.1.
X00075 mRNA. Translation: CAA24941.1.
X00076 mRNA. Translation: CAA24942.1.
X00076 mRNA. Translation: CAA24943.1. Sequence problems.
X02351
, X01396, X01397, X01398, X01399, X01400 Genomic DNA. Translation: CAA26206.1.
BC142365 mRNA. Translation: AAI42366.1.
BC151422 mRNA. Translation: AAI51423.1.
M68911 mRNA. Translation: AAA30724.1.
M68912 mRNA. Translation: AAA30725.1.
PIRiA05093. SPBOB.
JC5455.
RefSeqiNP_776618.1. NM_174193.2. [P01289-1]
XP_005205182.1. XM_005205125.3. [P01289-3]
XP_005205183.1. XM_005205126.3. [P01289-2]
XP_005205184.1. XM_005205127.3. [P01289-4]
XP_010802283.1. XM_010803981.2. [P01289-1]
UniGeneiBt.12930.

Genome annotation databases

EnsembliENSBTAT00000020419; ENSBTAP00000020419; ENSBTAG00000015356. [P01289-1]
ENSBTAT00000057037; ENSBTAP00000053014; ENSBTAG00000015356. [P01289-3]
ENSBTAT00000063529; ENSBTAP00000055772; ENSBTAG00000015356. [P01289-2]
GeneIDi281512.
KEGGibta:281512.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X00075 mRNA. Translation: CAA24939.1.
X00075 mRNA. Translation: CAA24940.1.
X00075 mRNA. Translation: CAA24941.1.
X00076 mRNA. Translation: CAA24942.1.
X00076 mRNA. Translation: CAA24943.1. Sequence problems.
X02351
, X01396, X01397, X01398, X01399, X01400 Genomic DNA. Translation: CAA26206.1.
BC142365 mRNA. Translation: AAI42366.1.
BC151422 mRNA. Translation: AAI51423.1.
M68911 mRNA. Translation: AAA30724.1.
M68912 mRNA. Translation: AAA30725.1.
PIRiA05093. SPBOB.
JC5455.
RefSeqiNP_776618.1. NM_174193.2. [P01289-1]
XP_005205182.1. XM_005205125.3. [P01289-3]
XP_005205183.1. XM_005205126.3. [P01289-2]
XP_005205184.1. XM_005205127.3. [P01289-4]
XP_010802283.1. XM_010803981.2. [P01289-1]
UniGeneiBt.12930.

3D structure databases

ProteinModelPortaliP01289.
SMRiP01289. Positions 72-107.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000020419.

Proteomic databases

PaxDbiP01289.
PRIDEiP01289.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000020419; ENSBTAP00000020419; ENSBTAG00000015356. [P01289-1]
ENSBTAT00000057037; ENSBTAP00000053014; ENSBTAG00000015356. [P01289-3]
ENSBTAT00000063529; ENSBTAP00000055772; ENSBTAG00000015356. [P01289-2]
GeneIDi281512.
KEGGibta:281512.

Organism-specific databases

CTDi6863.

Phylogenomic databases

eggNOGiENOG410IWZZ. Eukaryota.
ENOG4112BW1. LUCA.
GeneTreeiENSGT00390000002457.
HOGENOMiHOG000082514.
HOVERGENiHBG000127.
InParanoidiP01289.
KOiK05239.
OMAiEMGANDD.
OrthoDBiEOG7C2R3N.
TreeFamiTF333405.

Enzyme and pathway databases

ReactomeiR-BTA-380095. Tachykinin receptors bind tachykinins.
R-BTA-416476. G alpha (q) signalling events.

Miscellaneous databases

NextBioi20805479.

Family and domain databases

InterProiIPR013055. Tachy_Neuro_lke_CS.
IPR008215. Tachykinin_dom.
IPR008216. Tachykinin_fam.
[Graphical view]
PANTHERiPTHR11250. PTHR11250. 1 hit.
PRINTSiPR01829. PROTACHYKNIN.
SMARTiSM00203. TK. 2 hits.
[Graphical view]
PROSITEiPS00267. TACHYKININ. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequences of cloned cDNAs for two types of bovine brain substance P precursor."
    Nawa H., Hirose T., Takashima H., Inayama S., Nakanishi S.
    Nature 306:32-36(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS ALPHA AND BETA).
  2. "Tissue-specific generation of two preprotachykinin mRNAs from one gene by alternative RNA splicing."
    Nawa H., Kotani H., Nakanishi S.
    Nature 312:729-734(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], ALTERNATIVE SPLICING.
  3. NIH - Mammalian Gene Collection (MGC) project
    Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS BETA AND GAMMA).
    Strain: Crossbred X Angus.
    Tissue: Ileum and Liver.
  4. "Tachykinin (substance-P) gene expression in Leydig cells of the human and mouse testis."
    Chiwakata C., Brackmann B., Hunt N., Davidoff M., Schulze W., Ivell R.
    Endocrinology 128:2441-2448(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 36-122 (ISOFORMS BETA AND GAMMA).
    Tissue: Hypothalamus.
  5. "Quantitation and characterization of peptides from the C-terminal flanking region of rat and bovine preprotachykinins."
    McGregor G.P., Kage R., Thim L., Conlon J.M.
    J. Neurochem. 53:1871-1877(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 111-126 (ISOFORM BETA).

Entry informationi

Entry nameiTKN1_BOVIN
AccessioniPrimary (citable) accession number: P01289
Secondary accession number(s): A5PK57
, B0JYM8, P01291, P04091, P20773
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: April 13, 2016
This is version 130 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.