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P01286 (SLIB_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 135. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Somatoliberin
Alternative name(s):
Growth hormone-releasing factor
Short name=GRF
Growth hormone-releasing hormone
Short name=GHRH
Somatocrinin
Somatorelin
INN=Sermorelin
Gene names
Name:GHRH
Synonyms:GHRF
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length108 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

GRF is released by the hypothalamus and acts on the adenohypophyse to stimulate the secretion of growth hormone.

Subcellular location

Secreted.

Pharmaceutical use

Available under the names Groliberin (Pharmacia) or Somatrel (Ferring). Also available under the name Geref (Serono). Geref is a synthetic acetylated form of residues 1 to 29 of GHRH. Used for the treatment of growth hormone deficiency.

Sequence similarities

Belongs to the glucagon family.

Ontologies

Keywords
   Cellular componentSecreted
   Coding sequence diversityAlternative splicing
Polymorphism
   DomainSignal
   PTMAmidation
Cleavage on pair of basic residues
   Technical termComplete proteome
Direct protein sequencing
Pharmaceutical
Reference proteome
Gene Ontology (GO)
   Biological_processadenohypophysis development

Inferred from sequence or structural similarity. Source: BHF-UCL

adenylate cyclase-activating G-protein coupled receptor signaling pathway

Inferred from sequence or structural similarity. Source: BHF-UCL

cAMP-mediated signaling

Inferred from direct assay PubMed 7680413. Source: BHF-UCL

cell-cell signaling

Traceable author statement Ref.1. Source: ProtInc

growth hormone secretion

Inferred from direct assay PubMed 2857452. Source: MGI

positive regulation of cAMP biosynthetic process

Inferred from direct assay PubMed 1333056PubMed 7680413. Source: BHF-UCL

positive regulation of cell proliferation

Inferred from direct assay PubMed 12867592. Source: BHF-UCL

positive regulation of circadian sleep/wake cycle, REM sleep

Non-traceable author statement PubMed 18329818. Source: BHF-UCL

positive regulation of growth hormone secretion

Inferred from direct assay PubMed 18329818PubMed 8421089. Source: BHF-UCL

positive regulation of insulin-like growth factor receptor signaling pathway

Non-traceable author statement PubMed 11773624. Source: BHF-UCL

positive regulation of multicellular organism growth

Inferred from mutant phenotype PubMed 8421089. Source: BHF-UCL

response to food

Inferred from sequence or structural similarity. Source: BHF-UCL

   Cellular_componentextracellular region

Non-traceable author statement PubMed 11773624. Source: BHF-UCL

extracellular space

Inferred from sequence or structural similarity. Source: BHF-UCL

terminal bouton

Inferred from sequence or structural similarity. Source: BHF-UCL

   Molecular_functiongrowth hormone-releasing hormone activity

Inferred from direct assay PubMed 18329818PubMed 8421089. Source: BHF-UCL

growth hormone-releasing hormone receptor binding

Inferred from physical interaction PubMed 1333056PubMed 7680413. Source: BHF-UCL

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P01286-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P01286-2)

The sequence of this isoform differs from the canonical sequence as follows:
     103-103: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2020 Potential
Propeptide21 – 3111
PRO_0000011438
Peptide32 – 7544Somatoliberin Ref.6
PRO_0000011439
Propeptide78 – 10831
PRO_0000011440

Amino acid modifications

Modified residue751Leucine amide Ref.6

Natural variations

Alternative sequence1031Missing in isoform 2.
VSP_023146
Natural variant321Y → C.
Corresponds to variant rs17787698 [ dbSNP | Ensembl ].
VAR_049185
Natural variant751L → F.
Corresponds to variant rs4988492 [ dbSNP | Ensembl ].
VAR_024328

Experimental info

Sequence conflict921E → D in CAA24955. Ref.5

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified July 21, 1986. Version 1.
Checksum: 366AE05383488C53

FASTA10812,447
        10         20         30         40         50         60 
MPLWVFFFVI LTLSNSSHCS PPPPLTLRMR RYADAIFTNS YRKVLGQLSA RKLLQDIMSR 

        70         80         90        100 
QQGESNQERG ARARLGRQVD SMWAEQKQME LESILVALLQ KHSRNSQG 

« Hide

Isoform 2 [UniParc].

Checksum: 74432263093C5367
Show »

FASTA10712,360

References

« Hide 'large scale' references
[1]"Cloning and sequence analysis of cDNA for the precursor of human growth hormone-releasing factor, somatocrinin."
Gubler U., Monahan J.J., Lomedico P.T., Bhatt R.S., Collier K.J., Hoffman B.J., Boehlen P., Esch F., Ling N., Zeytin F., Brazeau P., Poonian M.S., Gage L.P.
Proc. Natl. Acad. Sci. U.S.A. 80:4311-4314(1983) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
[2]"Gene encoding human growth hormone-releasing factor precursor: structure, sequence, and chromosomal assignment."
Mayo K.E., Cerelli G.M., Lebo R.V., Bruce B.D., Rosenfeld M.G., Evans R.M.
Proc. Natl. Acad. Sci. U.S.A. 82:63-67(1985) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"The DNA sequence and comparative analysis of human chromosome 20."
Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P., Bird C.P., Blakey S.E. expand/collapse author list , Bridgeman A.M., Brown A.J., Buck D., Burrill W.D., Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G., Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E., Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D., Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P., Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E., Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J., Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D., Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S., Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D., Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A., Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T., Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I., Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M., Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D., Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M., Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A., Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L., Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L., Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J.
Nature 414:865-871(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
[5]"Expression-cloning and sequence of a cDNA encoding human growth hormone-releasing factor."
Mayo K.E., Vale W., Rivier J., Rosenfeld M.G., Evans R.M.
Nature 306:86-88(1983) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 6-101.
[6]"Growth hormone-releasing factor from a human pancreatic tumor that caused acromegaly."
Guillemin R., Brazeau P., Boehlen P., Esch F., Ling N., Wehrenberg W.B.
Science 218:585-587(1982) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 32-75, AMIDATION AT LEU-75.
[7]"Solution conformations of human growth hormone releasing factor: comparison of the restrained molecular dynamics and distance geometry methods for a system without long-range distance data."
Bruenger A.T., Clore G.M., Gronenborn A.M., Karplus M.
Protein Eng. 1:399-406(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY NMR OF 32-60.
[8]"Solution structure of human growth hormone releasing factor. Combined use of circular dichroism and nuclear magnetic resonance spectroscopy."
Clore G.M., Martin S.R., Gronenborn A.M.
J. Mol. Biol. 191:553-561(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY NMR OF 32-60.
+Additional computationally mapped references.

Web resources

Wikipedia

Growth hormone releasing hormone entry

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L29177 Genomic DNA. No translation available.
L00137 expand/collapse EMBL AC list , L00134, L00135, L00136 Genomic DNA. Translation: AAA52608.1.
L00137 expand/collapse EMBL AC list , L00134, L00135, L00136 Genomic DNA. Translation: AAA52609.1.
AL031659 Genomic DNA. Translation: CAB41762.2.
AL031659 Genomic DNA. Translation: CAI42673.1.
BC098109 mRNA. Translation: AAH98109.1.
BC098161 mRNA. Translation: AAH98161.1.
BC099727 mRNA. Translation: AAH99727.1.
X00094 mRNA. Translation: CAA24955.1.
X00094 mRNA. Translation: CAA24956.1.
PIRRHHUS. A21902.
RefSeqNP_001171660.1. NM_001184731.1.
NP_066567.1. NM_021081.4.
UniGeneHs.37023.

3D structure databases

ProteinModelPortalP01286.
SMRP01286. Positions 32-59, 78-104.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid108958. 1 interaction.
IntActP01286. 2 interactions.
MINTMINT-8090632.
STRING9606.ENSP00000237527.

PTM databases

PhosphoSiteP01286.

Polymorphism databases

DMDM134521.

Proteomic databases

PaxDbP01286.
PRIDEP01286.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000237527; ENSP00000237527; ENSG00000118702. [P01286-1]
ENST00000373611; ENSP00000362713; ENSG00000118702. [P01286-2]
ENST00000373614; ENSP00000362716; ENSG00000118702. [P01286-1]
GeneID2691.
KEGGhsa:2691.
UCSCuc002xgr.3. human. [P01286-1]
uc002xgt.3. human. [P01286-2]

Organism-specific databases

CTD2691.
GeneCardsGC20M035880.
HGNCHGNC:4265. GHRH.
MIM139190. gene.
neXtProtNX_P01286.
PharmGKBPA28675.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG47924.
HOGENOMHOG000115417.
HOVERGENHBG004478.
InParanoidP01286.
KOK05260.
OMAIMSRQQG.
OrthoDBEOG7WQ7VQ.
PhylomeDBP01286.
TreeFamTF353187.

Enzyme and pathway databases

ReactomeREACT_111102. Signal Transduction.

Gene expression databases

BgeeP01286.
CleanExHS_GHRH.
GenevestigatorP01286.

Family and domain databases

InterProIPR000532. Glucagon_GIP_secretin_VIP.
[Graphical view]
PfamPF00123. Hormone_2. 1 hit.
[Graphical view]
SMARTSM00070. GLUCA. 1 hit.
[Graphical view]
PROSITEPS00260. GLUCAGON. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi2691.
NextBio10640.
PROP01286.
SOURCESearch...

Entry information

Entry nameSLIB_HUMAN
AccessionPrimary (citable) accession number: P01286
Secondary accession number(s): Q4KN10, Q5JYR1
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: April 16, 2014
This is version 135 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 20

Human chromosome 20: entries, gene names and cross-references to MIM