P01282 (VIP_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
VIP peptides

Cleaved into the following 3 chains:

1. Intestinal peptide PHV-42
   Alternative name(s):
   Peptide histidine valine 42
2. Intestinal peptide PHM-27
   Alternative name(s):
   Peptide histidine methioninamide 27
3. Vasoactive intestinal peptide
   Short name=VIP
   Alternative name(s):
   Vasoactive intestinal polypeptide

Gene names

Name:

VIP

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	170 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	VIP causes vasodilation, lowers arterial blood pressure, stimulates myocardial contractility, increases glycogenolysis and relaxes the smooth muscle of trachea, stomach and gall bladder. PHM and PHV also cause vasodilation.
Subcellular location	Secreted.
Sequence similarities	Belongs to the glucagon family.

Ontologies

Keywords
Cellular component	Secreted
Coding sequence diversity	Alternative splicing
Domain	Signal
Molecular function	Hormone
PTM	Amidation Cleavage on pair of basic residues
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	body fluid secretion Traceable author statement PubMed 4035357. Source: ProtInc learning or memory Inferred from electronic annotation. Source: Compara negative regulation of apoptotic process Inferred from electronic annotation. Source: Compara negative regulation of potassium ion transport Inferred from electronic annotation. Source: Compara negative regulation of smooth muscle cell proliferation Inferred from electronic annotation. Source: Compara positive regulation of adenylate cyclase activity involved in G-protein coupled receptor signaling pathway Inferred from direct assay PubMed 9603988. Source: BHF-UCL positive regulation of cell proliferation Traceable author statement PubMed 8389448. Source: ProtInc positive regulation of endothelial cell proliferation Inferred from electronic annotation. Source: Compara positive regulation of protein catabolic process Inferred from direct assay PubMed 9603988. Source: BHF-UCL positive regulation of vasodilation Inferred from electronic annotation. Source: Compara regulation of protein localization Inferred from direct assay PubMed 9603988. Source: BHF-UCL regulation of sensory perception of pain Inferred from electronic annotation. Source: Compara regulation of signal transduction Inferred from electronic annotation. Source: Compara
Cellular_component	extracellular region Traceable author statement. Source: Reactome neuronal cell body Inferred from electronic annotation. Source: Compara
Molecular_function	neuropeptide hormone activity Traceable author statement PubMed 10096039. Source: ProtInc
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P01282-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P01282-2)

The sequence of this isoform differs from the canonical sequence as follows:
     113-113: Missing.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 20

20

Potential

Propeptide

21 – 79

59

PRO_0000011457

Peptide

81 – 122

42

Intestinal peptide PHV-42 Ref.11

PRO_0000011458

Peptide

81 – 107

27

Intestinal peptide PHM-27

PRO_0000011459

Peptide

125 – 152

28

Vasoactive intestinal peptide

PRO_0000011460

Propeptide

156 – 170

15

PRO_0000011461

Amino acid modifications

Modified residue

107

1

Methionine amide

Modified residue

152

1

Asparagine amide

Natural variations

Alternative sequence

113

1

Missing in isoform 2.

VSP_023256

Experimental info

Sequence conflict

96 – 97

2

QL → PP in AAA61286. Ref.8

Sequence conflict

116

1

S → L in AAA61288. Ref.4

Sequence conflict

136

1

R → G in AAA61288. Ref.4

Secondary structure

1

.

.

.

170

Helix Strand Turn

Details...

Helix

128 – 152

25

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified July 21, 1986. Version 1. Checksum: 93EC0177F89508FD	FASTA	170	19,169
	10 20 30 40 50 60 MDTRNKAQLL VLLTLLSVLF SQTSAWPLYR APSALRLGDR IPFEGANEPD QVSLKEDIDM 70 80 90 100 110 120 LQNALAENDT PYYDVSRNAR HADGVFTSDF SKLLGQLSAK KYLESLMGKR VSSNISEDPV 130 140 150 160 170 PVKRHSDAVF TDNYTRLRKQ MAVKKYLNSI LNGKRSSEGE SPDFPEELEK
	Isoform 2 [UniParc]. Checksum: F325BDFEF47132C3	FASTA	169	19,082
	10 20 30 40 50 60 MDTRNKAQLL VLLTLLSVLF SQTSAWPLYR APSALRLGDR IPFEGANEPD QVSLKEDIDM 70 80 90 100 110 120 LQNALAENDT PYYDVSRNAR HADGVFTSDF SKLLGQLSAK KYLESLMGKR VSNISEDPVP 130 140 150 160 VKRHSDAVFT DNYTRLRKQM AVKKYLNSIL NGKRSSEGES PDFPEELEK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Human preprovasoactive intestinal polypeptide contains a novel PHI-27-like peptide, PHM-27." Itoh N., Obata K., Yanaihara N., Okamoto H. Nature 304:547-549(1983) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Structure of the human vasoactive intestinal polypeptide gene." Tsukada T., Horovitch S.J., Montminy M.R., Mandel G., Goodman R.H. DNA 4:293-300(1985) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Vasoactive intestinal peptide: expression of the prohormone in bacterial cells." Delamarter J.F., Buell G.N., Kawashima E., Polak J.M., Bloom S.R. Peptides 6 Suppl. 1:95-102(1985) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[4]	"Structure and expression of the gene encoding the vasoactive intestinal peptide precursor." Linder S., Barkhem T., Norberg A., Persson H., Schalling M., Hoekfelt T., Magnusson G. Proc. Natl. Acad. Sci. U.S.A. 84:605-609(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[5]	"Complete nucleotide sequence of human vasoactive intestinal peptide/PHM-27 gene and its inducible promoter." Yamagami T., Ohsawa K., Nishizawa M., Inoue C., Gotoh E., Yanaihara N., Yamamoto H., Okamoto H. Ann. N. Y. Acad. Sci. 527:87-102(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[6]	"The DNA sequence and analysis of human chromosome 6." Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D. , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S. Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Prostate.
[8]	"Structure and expression of the vasoactive intestinal peptide (VIP) gene in a human tumor." Gozes I., Bodener M., Shani Y., Fridkin M. Peptides 7:1-6(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 8-170.
[9]	"Diarrhoea in vipoma patients associated with cosecretion of a second active peptide (peptide histidine isoleucine) explained by single coding gene." Bloom S.R., Delamarter J.F., Kawashima E., Christofides N.D., Buell G., Polak J.M. Lancet 2:1163-1165(1983) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 50-170 (ISOFORM 1). Tissue: Pancreatic carcinoma.
[10]	"Vasoactive intestinal peptide gene: putative mechanism of information storage at the RNA level." Gozes I., Giladi E., Shani Y. J. Neurochem. 48:1136-1141(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 78-155.
[11]	"Isolation, characterization, and pharmacological actions of peptide histidine valine 42, a novel prepro-vasoactive intestinal peptide-derived peptide." Yiangou Y., di Marzo V., Spokes R.A., Panico M., Morris H.R., Bloom S.R. J. Biol. Chem. 262:14010-14013(1987) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 81-122.
[12]	"Isolation and characterization of peptides which act on rat platelets, from a pheochromocytoma." Kitamura K., Kangawa K., Kawamoto M., Ichiki Y., Matsuo H., Eto T. Biochem. Biophys. Res. Commun. 185:134-141(1992) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 127-152. Tissue: Pheochromocytoma.
[13]	"Structural determination of the vasoactive intestinal peptide by two-dimensional H-NMR spectroscopy." Theriault Y., Boulanger Y., St Pierre S. Biopolymers 31:459-464(1991) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY NMR OF VIP.
+	Additional computationally mapped references.

Web resources

Wikipedia

Vasoactive intestinal peptide entry

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

L00157 , L00154, L00155, L00156 Genomic DNA. Translation: AAA61289.1.
M11553 , M11549, M11550, M11551, M11552 Genomic DNA. Translation: AAA61284.1.
M36634 mRNA. Translation: AAA61287.1.
M14623 , M14619, M14620, M14621, M14622 Genomic DNA. Translation: AAA61288.1.
M33027 Genomic DNA. Translation: AAA69515.1.
AL133356 Genomic DNA. Translation: CAI21764.1.
AL133356 Genomic DNA. Translation: CAI21765.1.
BC009794 mRNA. Translation: AAH09794.1.
M36610 , M36606, M36607, M36608, M36609 Genomic DNA. Translation: AAA61286.1.
M54930 mRNA. Translation: AAA63268.1.
M32162, M31645 Genomic DNA. Translation: AAA61285.1.

IPI

IPI00000959.
IPI00375701.

PIR

VRHU. A23296.

RefSeq

NP_003372.1. NM_003381.3.
NP_919416.1. NM_194435.2.

UniGene

Hs.53973.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2RRH	NMR	-	A	125-153	[»]
2RRI	NMR	-	A	125-153	[»]

ProteinModelPortal

P01282.

ModBase

Search...

Protein-protein interaction databases

IntAct

P01282. 2 interactions.

MINT

MINT-1473277.

STRING

9606.ENSP00000356213.

PTM databases

PhosphoSite

P01282.

Polymorphism databases

DMDM

138574.

Proteomic databases

PaxDb

P01282.

PRIDE

P01282.

Protocols and materials databases

DNASU

7432.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000367243; ENSP00000356212; ENSG00000146469.
ENST00000367244; ENSP00000356213; ENSG00000146469.

GeneID

7432.

KEGG

hsa:7432.

UCSC

uc003qpe.3. human.
uc003qpf.3. human.

Organism-specific databases

CTD

7432.

GeneCards

GC06P153164.

HGNC

HGNC:12693. VIP.

HPA

CAB018649.
HPA017324.

MIM

192320. gene.

neXtProt

NX_P01282.

PharmGKB

PA37312.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG44083.

HOGENOM

HOG000253943.

HOVERGEN

HBG018069.

InParanoid

P01282.

KO

K05264.

OMA

METRSKP.

OrthoDB

EOG4KPTCC.

PhylomeDB

P01282.

Enzyme and pathway databases

Reactome

REACT_111102. Signal Transduction.

Gene expression databases

ArrayExpress

P01282.

Bgee

P01282.

CleanEx

HS_VIP.

Genevestigator

P01282.

GermOnline

ENSG00000146469. Homo sapiens.

Family and domain databases

InterPro

IPR000532. Glucagon_GIP_secretin_VIP.
IPR015523. VIP.
[Graphical view]

PANTHER

PTHR11213:SF2. PTHR11213:SF2. 1 hit.

Pfam

PF00123. Hormone_2. 2 hits.
[Graphical view]

SMART

SM00070. GLUCA. 2 hits.
[Graphical view]

PROSITE

PS00260. GLUCAGON. 2 hits.
[Graphical view]

ProtoNet

Search...

Other

BindingDB

P01282.

ChEMBL

CHEMBL5737.

EvolutionaryTrace

P01282.

GenomeRNAi

7432.

NextBio

29108.

SOURCE

Search...

Entry information

Entry name

VIP_HUMAN

Accession

Primary (citable) accession number: P01282
Secondary accession number(s): Q5TCY8, Q5TCY9, Q96QK3

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	May 1, 2013
This is version 138 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.