Reviewed, UniProtKB/Swiss-Prot P01256 (CALCA_RAT)

Names and originHide

Protein names

Recommended name:
Calcitonin gene-related peptide 1

Alternative name(s):
Calcitonin gene-related peptide I
Short name=CGRP-I
Alpha-type CGRP

Gene names

Name:	Calca
Synonyms:	Calc

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributesHide

Sequence length	128 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)Hide

Function	CGRP induces vasodilation. It dilates a variety of vessels including the coronary, cerebral and systemic vasculature. Its abundance in the CNS also points toward a neurotransmitter or neuromodulator role.
Subcellular location	Secreted.
Sequence similarities	Belongs to the calcitonin family.

OntologiesHide

Keywords
Cellular component	Secreted
Coding sequence diversity	Alternative splicing
Domain	Signal
Molecular function	Hormone
PTM	Amidation Cleavage on pair of basic residues Disulfide bond
Gene Ontology (GO)
Biological process	aging Inferred from expression pattern. Source: RGD negative regulation of blood pressure Inferred from mutant phenotype. Source: RGD negative regulation of smooth muscle contraction Inferred from mutant phenotype. Source: RGD regulation of heart contraction Inferred from direct assay. Source: RGD regulation of inflammatory response Inferred from mutant phenotype. Source: RGD vasodilation Inferred from direct assay. Source: RGD
Cellular component	axon Inferred from direct assay. Source: RGD extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from direct assay. Source: RGD
Molecular function	calcitonin receptor binding Inferred from mutant phenotype. Source: RGD neuropeptide hormone activity Traceable author statement. Source: RGD
Complete GO annotation...

Alternative productsHide

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform Calcitonin-gene related peptide I (identifier: P01256-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Calcitonin (identifier: P01257-1)

The sequence of this isoform can be found in the external entry P01257.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Sequence annotation (Features)Hide

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 25	25
	Propeptide	26 – 80	55			PRO_0000004065
	Peptide	83 – 119	37	Calcitonin gene-related peptide 1		PRO_0000004066
	Propeptide	125 – 128	4			PRO_0000004067
Amino acid modifications
	Modified residue	119	1	Phenylalanine amide By similarity
	Disulfide bond	84 ↔ 89		By similarity
Experimental info
	Sequence conflict	40	1	Missing Ref.2
	Sequence conflict	40	1	Missing Ref.3
	Sequence conflict	51	1	Missing Ref.2
	Sequence conflict	51	1	Missing Ref.3
	Sequence conflict	70	1	Q → EEQ Ref.2
	Sequence conflict	70	1	Q → EEQ Ref.3
	Sequence conflict	99	1	S → R in AAA40847. Ref.3

SequencesHide

	Sequence		Length	Mass (Da)
	Isoform Calcitonin-gene related peptide I [UniParc]. Last modified April 1, 1988. Version 1. Checksum: 75D14869C17078D3	FASTA	128	13,948
	10 20 30 40 50 60 MGFLKFSPFL VVSILLLYQA CGLQAVPLRS TLESSPGMAA TLSEEEARLL LAALVQNYMQ 70 80 90 100 110 120 MKVRELEQEQ EAEGSSVTAQ KRSCNTATCV THRLAGLLSR SGGVVKDNFV PTNVGSEAFG RRRRDLQA
	Isoform Calcitonin. See P01257.	FASTA	–	–

ReferencesHide

[1]	"Alternative RNA processing events in human calcitonin/calcitonin gene-related peptide gene expression." Jonas V., Lin C.R., Kawashima E., Semon D., Swanson L.W., Mermod J.-J., Evans R.M., Rosenfeld M.G. Proc. Natl. Acad. Sci. U.S.A. 82:1994-1998(1985) [PubMed: 3872459] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
[2]	"Alternative RNA processing in calcitonin gene expression generates mRNAs encoding different polypeptide products." Amara S.G., Jonas V., Rosenfeld M.G., Ong E.S., Evans R.M. Nature 298:240-244(1982) [PubMed: 6283379] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Expression in brain of a messenger RNA encoding a novel neuropeptide homologous to calcitonin gene-related peptide." Amara S.G., Arriza J.L., Leff S.E., Swanson L.W., Evans R.M., Rosenfeld M.G. Science 229:1094-1097(1985) [PubMed: 2994212] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
+	Additional computationally mapped references.

Cross-referencesHide

Sequence databases
EMBL GenBank DDBJ	L29188, L00109, L00110 Genomic DNA. Translation: AAB59682.1. V01231 mRNA. Translation: CAA24541.1. M11597 mRNA. Translation: AAA40847.1.
IPI	IPI00211710.
PIR	TCRTR. A01524. B44173.
RefSeq	NP_001029127.1. NP_001029128.1.
UniGene	Rn.90085
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	P01256.
Genome annotation databases
GeneID	24241.
KEGG	rno:24241.
UCSC	NM_001033955. rat.
Organism-specific databases
CTD	24241.
RGD	2254. Calca.
Phylogenomic databases
eggNOG	roNOG17038.
HOVERGEN	HBG031362.
Gene expression databases
Genevestigator	P01256.
Family and domain databases
InterPro	IPR018360. Calcitonin_CS. IPR001693. Calcitonin_peptide-like. IPR021117. Procalcitonin-like. IPR021116. Procalcitonin/adrenomedullin. [Graphical view]
PANTHER	PTHR10505. Calcitonin-like. 1 hit.
Pfam	PF00214. Calc_CGRP_IAPP. 1 hit. [Graphical view]
SMART	SM00113. CALCITONIN. 1 hit. [Graphical view]
PROSITE	PS00258. CALCITONIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry informationHide

Entry name

CALCA_RAT

Accession

Primary (citable) accession number: P01256

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	April 1, 1988
Last modified:	July 13, 2010
This is version 85 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)