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Protein

Prolactin

Gene

PRL

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Prolactin acts primarily on the mammary gland by promoting lactation.

GO - Molecular functioni

GO - Biological processi

  • biosynthetic process Source: AgBase
  • blastocyst formation Source: AgBase
  • lactation Source: UniProtKB-KW
  • long-day photoperiodism Source: AgBase
  • negative regulation of apoptotic process Source: AgBase
  • negative regulation of gene expression Source: AgBase
  • negative regulation of nitric oxide mediated signal transduction Source: AgBase
  • peptide hormone secretion Source: AgBase
  • positive regulation of endocytosis Source: AgBase
  • positive regulation of fatty acid biosynthetic process Source: AgBase
  • positive regulation of gene expression Source: AgBase
  • positive regulation of lactation Source: AgBase
  • positive regulation of NF-kappaB import into nucleus Source: AgBase
  • positive regulation of NF-kappaB transcription factor activity Source: AgBase
  • positive regulation of nitric oxide biosynthetic process Source: AgBase
  • regulation of meiotic cell cycle process involved in oocyte maturation Source: AgBase
  • response to external biotic stimulus Source: AgBase
  • response to food Source: AgBase
  • response to L-arginine Source: AgBase
  • response to mechanical stimulus Source: AgBase
  • signal transduction involved in regulation of gene expression Source: AgBase
Complete GO annotation...

Keywords - Molecular functioni

Hormone

Keywords - Biological processi

Lactation

Enzyme and pathway databases

ReactomeiR-BTA-1170546. Prolactin receptor signaling.
R-BTA-982772. Growth hormone receptor signaling.

Names & Taxonomyi

Protein namesi
Recommended name:
Prolactin
Short name:
PRL
Gene namesi
Name:PRL
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 23

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 30301 PublicationAdd
BLAST
Chaini31 – 229199ProlactinPRO_0000032911Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi34 ↔ 411 Publication
Modified residuei56 – 561Phosphoserine1 Publication
Modified residuei64 – 641Phosphoserine1 Publication
Disulfide bondi88 ↔ 2041 Publication
Modified residuei120 – 1201Phosphoserine1 Publication
Disulfide bondi221 ↔ 2291 Publication

Keywords - PTMi

Disulfide bond, Phosphoprotein

Proteomic databases

PaxDbiP01239.
PRIDEiP01239.

PTM databases

iPTMnetiP01239.

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000020313.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3JC2electron microscopy3.60w12-30[»]
ProteinModelPortaliP01239.
SMRiP01239. Positions 32-229.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the somatotropin/prolactin family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410II74. Eukaryota.
ENOG410XU1S. LUCA.
GeneTreeiENSGT00730000110805.
HOGENOMiHOG000264241.
HOVERGENiHBG104895.
InParanoidiP01239.
KOiK05439.
OMAiLHCLRRD.
OrthoDBiEOG74FF24.
TreeFamiTF332592.

Family and domain databases

Gene3Di1.20.1250.10. 1 hit.
InterProiIPR009079. 4_helix_cytokine-like_core.
IPR012351. 4_helix_cytokine_core.
IPR001400. Somatotropin.
IPR018116. Somatotropin_CS.
[Graphical view]
PANTHERiPTHR11417. PTHR11417. 1 hit.
PfamiPF00103. Hormone_1. 1 hit.
[Graphical view]
PRINTSiPR00836. SOMATOTROPIN.
SUPFAMiSSF47266. SSF47266. 1 hit.
PROSITEiPS00266. SOMATOTROPIN_1. 1 hit.
PS00338. SOMATOTROPIN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P01239-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDSKGSSQKG SRLLLLLVVS NLLLCQGVVS TPVCPNGPGN CQVSLRDLFD
60 70 80 90 100
RAVMVSHYIH DLSSEMFNEF DKRYAQGKGF ITMALNSCHT SSLPTPEDKE
110 120 130 140 150
QAQQTHHEVL MSLILGLLRS WNDPLYHLVT EVRGMKGAPD AILSRAIEIE
160 170 180 190 200
EENKRLLEGM EMIFGQVIPG AKETEPYPVW SGLPSLQTKD EDARYSAFYN
210 220
LLHCLRRDSS KIDTYLKLLN CRIIYNNNC
Length:229
Mass (Da):25,793
Last modified:July 21, 1986 - v1
Checksum:iE7E9BB6655A26F3D
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti61 – 611D → N AA sequence (PubMed:4608931).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V00112 mRNA. Translation: CAA23446.1.
BC148124 mRNA. Translation: AAI48125.1.
AF426315 Genomic DNA. Translation: AAL28075.1.
M36873 mRNA. Translation: AAA30737.1.
M36874 mRNA. Translation: AAA30738.1.
X14320 mRNA. Translation: CAA32500.1.
X14321 mRNA. Translation: CAA32501.1.
X01452 Genomic DNA. Translation: CAB57794.1.
X01744 Genomic DNA. Translation: CAA25880.1.
PIRiA92378. LCBO.
RefSeqiNP_776378.2. NM_173953.2.
UniGeneiBt.7197.

Genome annotation databases

EnsembliENSBTAT00000020313; ENSBTAP00000020313; ENSBTAG00000015274.
GeneIDi280901.
KEGGibta:280901.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V00112 mRNA. Translation: CAA23446.1.
BC148124 mRNA. Translation: AAI48125.1.
AF426315 Genomic DNA. Translation: AAL28075.1.
M36873 mRNA. Translation: AAA30737.1.
M36874 mRNA. Translation: AAA30738.1.
X14320 mRNA. Translation: CAA32500.1.
X14321 mRNA. Translation: CAA32501.1.
X01452 Genomic DNA. Translation: CAB57794.1.
X01744 Genomic DNA. Translation: CAA25880.1.
PIRiA92378. LCBO.
RefSeqiNP_776378.2. NM_173953.2.
UniGeneiBt.7197.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3JC2electron microscopy3.60w12-30[»]
ProteinModelPortaliP01239.
SMRiP01239. Positions 32-229.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000020313.

PTM databases

iPTMnetiP01239.

Proteomic databases

PaxDbiP01239.
PRIDEiP01239.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000020313; ENSBTAP00000020313; ENSBTAG00000015274.
GeneIDi280901.
KEGGibta:280901.

Organism-specific databases

CTDi5617.

Phylogenomic databases

eggNOGiENOG410II74. Eukaryota.
ENOG410XU1S. LUCA.
GeneTreeiENSGT00730000110805.
HOGENOMiHOG000264241.
HOVERGENiHBG104895.
InParanoidiP01239.
KOiK05439.
OMAiLHCLRRD.
OrthoDBiEOG74FF24.
TreeFamiTF332592.

Enzyme and pathway databases

ReactomeiR-BTA-1170546. Prolactin receptor signaling.
R-BTA-982772. Growth hormone receptor signaling.

Miscellaneous databases

NextBioi20805030.

Family and domain databases

Gene3Di1.20.1250.10. 1 hit.
InterProiIPR009079. 4_helix_cytokine-like_core.
IPR012351. 4_helix_cytokine_core.
IPR001400. Somatotropin.
IPR018116. Somatotropin_CS.
[Graphical view]
PANTHERiPTHR11417. PTHR11417. 1 hit.
PfamiPF00103. Hormone_1. 1 hit.
[Graphical view]
PRINTSiPR00836. SOMATOTROPIN.
SUPFAMiSSF47266. SSF47266. 1 hit.
PROSITEiPS00266. SOMATOTROPIN_1. 1 hit.
PS00338. SOMATOTROPIN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence of bovine prolactin messenger RNA. Evidence for sequence polymorphism."
    Sasavage N.L., Nilson J.H., Horowitz S., Rottman F.M.
    J. Biol. Chem. 257:678-681(1982) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Molecular cloning and analysis of bovine prolactin full-long genomic as well as cDNA sequences."
    Cao X., Wang Q., Yan J.B., Yang F.K., Huang S.Z., Zeng Y.T.
    Yi Chuan Xue Bao 29:768-773(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Tissue: Blood.
  3. NIH - Mammalian Gene Collection (MGC) project
    Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Fetal medulla.
  4. "Genetic engineering of peptide hormones. II. Possible polymorphism of preprolactin in cattle. Data of molecular cloning."
    Rubtsov P.M., Oganesyan R.G., Gorbulev V.G., Skryabin K.G., Baev A.A.
    Mol. Biol. (Mosk.) 22:117-127(1988)
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-68 AND 96-229.
  5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-38.
  6. "Cloning of bovine prolactin cDNA and evolutionary implications of its sequence."
    Miller W.L., Coit D., Baxter J.D., Martial J.A.
    DNA 1:37-50(1981) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 21-229.
  7. "Bovine prolactin: corrected cDNA sequence and genetic polymorphisms."
    Miller W.L., Coit D., Baxter J.D., Martial J.A.
    DNA 1:313-314(1982) [PubMed] [Europe PMC] [Abstract]
    Cited for: SEQUENCE REVISION.
  8. "The primary structure of bovine prolactin."
    Wallis M.
    FEBS Lett. 44:205-208(1974) [PubMed] [Europe PMC] [Abstract]
    Cited for: PRELIMINARY PROTEIN SEQUENCE OF 31-229.
  9. "An evidence for deamidation of prolactin monomer."
    Graf L., Cseh G., Nagy I., Kurcz M.
    Acta Biochim. Biophys. Acad. Sci. Hung. 5:299-303(1970) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 31-46.
  10. "Isolation and characterization of phosphorylated bovine prolactin."
    Kim B.G., Brooks C.L.
    Biochem. J. 296:41-47(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 52-72 AND 120-133, PHOSPHORYLATION AT SER-56; SER-64 AND SER-120.
    Tissue: Pituitary.

Entry informationi

Entry nameiPRL_BOVIN
AccessioniPrimary (citable) accession number: P01239
Secondary accession number(s): A6QLX8, Q29417, Q95112
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: March 16, 2016
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.