P01222 (TSHB_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thyrotropin subunit beta

Alternative name(s):
Thyroid-stimulating hormone subunit beta
Short name=TSH-B
Short name=TSH-beta
Thyrotropin beta chain
INN=Thyrotropin alfa

Gene names

Name:

TSHB

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	138 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Indispensable for the control of thyroid structure and metabolism.
Subunit structure	Heterodimer of a common alpha chain and a unique beta chain which confers biological specificity to thyrotropin, lutropin, follitropin and gonadotropin.
Subcellular location	Secreted.
Pharmaceutical use	Available under the name Thyrogen (Genzyme). Used in combination with other tests to detect recurring or leftover thyroid cancer cells in patients with a history of certain types of thyroid cancer.
Sequence similarities	Belongs to the glycoprotein hormones subunit beta family.

Ontologies

Keywords
Cellular component	Secreted
Coding sequence diversity	Polymorphism
Domain	Signal
Molecular function	Hormone
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Direct protein sequencing Pharmaceutical Reference proteome
Gene Ontology (GO)
Biological_process	G-protein coupled receptor signaling pathway Traceable author statement PubMed 2792087. Source: ProtInc anatomical structure morphogenesis Traceable author statement PubMed 2792087. Source: ProtInc cell-cell signaling Traceable author statement PubMed 2792087. Source: ProtInc cellular nitrogen compound metabolic process Traceable author statement. Source: Reactome peptide hormone processing Traceable author statement. Source: Reactome response to calcium ion Inferred from electronic annotation. Source: Compara response to estrogen stimulus Inferred from electronic annotation. Source: Compara response to vitamin A Inferred from electronic annotation. Source: Compara small molecule metabolic process Traceable author statement. Source: Reactome
Cellular_component	extracellular region Traceable author statement. Source: Reactome
Molecular_function	hormone activity Traceable author statement PubMed 2792087. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	Ref.8
	Chain	21 – 132	112	Thyrotropin subunit beta		PRO_0000011746
	Propeptide	133 – 138	6			PRO_0000011747
Amino acid modifications
	Glycosylation	43	1	N-linked (GlcNAc...)
	Disulfide bond	22 ↔ 72		By similarity
	Disulfide bond	36 ↔ 87		By similarity
	Disulfide bond	39 ↔ 125		By similarity
	Disulfide bond	47 ↔ 103		By similarity
	Disulfide bond	51 ↔ 105		By similarity
	Disulfide bond	108 ↔ 115		By similarity
Natural variations
	Natural variant	14	1	T → A. Ref.1 Ref.2 Ref.3 Ref.4 Ref.5 Ref.7 Corresponds to variant rs10776792 [ dbSNP | Ensembl ].		VAR_054769
Experimental info
	Sequence conflict	46	1	I → M in AAB30828. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	P01222 [UniParc]. Last modified November 2, 2010. Version 2. Checksum: 6A367538D8393DB7	FASTA	138	15,639
	10 20 30 40 50 60 MTALFLMSML FGLTCGQAMS FCIPTEYTMH IERRECAYCL TINTTICAGY CMTRDINGKL 70 80 90 100 110 120 FLPKYALSQD VCTYRDFIYR TVEIPGCPLH VAPYFSYPVA LSCKCGKCNT DYSDCIHEAI 130 KTNYCTKPQK SYLVGFSV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning of the human thyrotropin-beta subunit gene." Hayashizaki Y., Miyai K., Kato K., Matsubara K. FEBS Lett. 188:394-400(1985) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT ALA-14.
[2]	"The human thyrotropin beta-subunit gene differs in 5' structure from murine TSH-beta genes." Guidon P.T. Jr., Whitfield G.K., Porti D., Kourides I.A. DNA 7:691-699(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT ALA-14.
[3]	"The structure of the human thyrotropin beta-subunit gene." Tatsumi K., Hayashizaki Y., Hiraoka Y., Miyai K., Matsubara K. Gene 73:489-497(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT ALA-14.
[4]	"Isolation and characterization of the human thyrotropin beta-subunit gene. Differences in gene structure and promoter function from murine species." Wondisford F.E., Radovick S., Moates J.M., Usala S.J., Weintraub B.D. J. Biol. Chem. 263:12538-12542(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT ALA-14.
[5]	"Structure and regulation of human thyroid-stimulating hormone (TSH) gene." Miyoshi I., Kasai N., Hayashizaki Y. Nippon Rinsho 52:940-947(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT ALA-14.
[6]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ALA-14.
[8]	"Human pituitary thyrotropin. The primary structure of the alpha and beta subunits." Sairam M.R., Li C.H. Can. J. Biochem. 55:755-760(1977) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 21-132.
+	Additional computationally mapped references.

Web resources

Thyrogen

Clinical information on Thyrogen

Wikipedia

Thyroid-stimulating hormone entry

Cross-references

Sequence databases
EMBL GenBank DDBJ	X02866 Genomic DNA. Translation: CAA26618.1. X02867 Genomic DNA. Translation: CAA26619.1. M23671, M23670 Genomic DNA. Translation: AAB05845.1. M25164 Genomic DNA. Translation: AAA61235.1. M21024 Genomic DNA. Translation: AAA36782.1. S70587 Genomic DNA. Translation: AAB30828.2. AL109660 Genomic DNA. Translation: CAI22270.1. BC069298 mRNA. Translation: AAH69298.1.
IPI	IPI00000851.
PIR	TTHUB. A23997.
RefSeq	NP_000540.2. NM_000549.3.
UniGene	Hs.406687.
3D structure databases
ProteinModelPortal	P01222.
SMR	P01222. Positions 22-115.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000256592.
PTM databases
PhosphoSite	P01222.
Polymorphism databases
DMDM	311033515.
Proteomic databases
PaxDb	P01222.
PRIDE	P01222.
Protocols and materials databases
DNASU	7252.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000256592; ENSP00000256592; ENSG00000134200. ENST00000369517; ENSP00000358530; ENSG00000134200.
GeneID	7252.
KEGG	hsa:7252.
UCSC	uc001efs.1. human.
Organism-specific databases
CTD	7252.
GeneCards	GC01P115572.
H-InvDB	HIX0028781.
HGNC	HGNC:12372. TSHB.
MIM	188540. gene. 275100. phenotype.
neXtProt	NX_P01222.
Orphanet	90674. Isolated thyroid-stimulating hormone deficiency.
PharmGKB	PA37041.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG40888.
HOGENOM	HOG000116098.
HOVERGEN	HBG006698.
InParanoid	P01222.
KO	K05251.
OMA	TNYCTKP.
OrthoDB	EOG4PNXJ6.
PhylomeDB	P01222.
Enzyme and pathway databases
Reactome	REACT_111102. Signal Transduction. REACT_111217. Metabolism.
Gene expression databases
Bgee	P01222.
CleanEx	HS_TSHB.
Genevestigator	P01222.
GermOnline	ENSG00000134200. Homo sapiens.
Family and domain databases
InterPro	IPR006208. Cys_knot. IPR001545. Gonadotropin_bsu. IPR018245. Gonadotropin_bsu_CS. [Graphical view]
PANTHER	PTHR11515. PTHR11515. 1 hit.
Pfam	PF00007. Cys_knot. 1 hit. [Graphical view]
SMART	SM00068. GHB. 1 hit. [Graphical view]
PROSITE	PS00261. GLYCO_HORMONE_BETA_1. 1 hit. PS00689. GLYCO_HORMONE_BETA_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	7252.
NextBio	28357.
SOURCE	Search...

Entry information

Entry name

TSHB_HUMAN

Accession

Primary (citable) accession number: P01222
Secondary accession number(s): B1AKP0, Q16163

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	November 2, 2010
Last modified:	May 1, 2013
This is version 121 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.