P01218 (GLHA_SHEEP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycoprotein hormones alpha chain

Alternative name(s):
Anterior pituitary glycoprotein hormones common subunit alpha
Follicle-stimulating hormone alpha chain
Short name=FSH-alpha
Follitropin alpha chain
LH alpha 3
Luteinizing hormone alpha chain
Short name=LSH-alpha
Lutropin alpha chain
Thyroid-stimulating hormone alpha chain
Short name=TSH-alpha
Thyrotropin alpha chain

Cleaved into the following 3 chains:

1. LH alpha 1-1
2. LH alpha 1-2
3. LH alpha 1-3

Gene names

Name:

CGA

Organism

Ovis aries (Sheep)

Taxonomic identifier

9940 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Caprinae › Ovis

Protein attributes

Sequence length	120 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Subunit structure	Heterodimer of a common alpha chain and a unique beta chain which confers biological specificity to thyrotropin, lutropin, follitropin and gonadotropin. The alpha chain can also be formed by LH alpha 1-1, LH alpha 1-2 and LH alpha 1-3. Ref.5
Subcellular location	Secreted.
Sequence similarities	Belongs to the glycoprotein hormones subunit alpha family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Hormone
PTM	Disulfide bond Glycoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24	Ref.2 Ref.3 Ref.4 Ref.5
	Chain	25 – 120	96	Glycoprotein hormones alpha chain		PRO_0000011652
	Chain	28 – 120	93	LH alpha 1-1		PRO_0000011653
	Chain	30 – 120	91	LH alpha 1-2		PRO_0000011654
	Chain	31 – 120	90	LH alpha 1-3		PRO_0000011655
Amino acid modifications
	Glycosylation	80	1	N-linked (GlcNAc...)		CAR_000038
	Glycosylation	106	1	N-linked (GlcNAc...)		CAR_000039
	Disulfide bond	35 ↔ 59		By similarity
	Disulfide bond	38 ↔ 88		By similarity
	Disulfide bond	56 ↔ 110		By similarity
	Disulfide bond	60 ↔ 112		By similarity
	Disulfide bond	87 ↔ 115		By similarity
Experimental info
	Sequence conflict	27	1	D → N AA sequence Ref.2
	Sequence conflict	29	1	E → Q AA sequence Ref.2
	Sequence conflict	37	1	E → Q AA sequence Ref.2
	Sequence conflict	37	1	E → Q AA sequence Ref.3
	Sequence conflict	37	1	E → Q AA sequence Ref.4
	Sequence conflict	112 – 113	2	CS → SC AA sequence Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P01218 [UniParc]. Last modified April 1, 1990. Version 2. Checksum: 1F06F784F2420181	FASTA	120	13,588
	10 20 30 40 50 60 MDYYRKYAAA ILAILSLFLQ ILHSFPDGEF TMQGCPECKL KENKYFSKPD APIYQCMGCC 70 80 90 100 110 120 FSRAYPTPAR SKKTMLVPKN ITSEATCCVA KAFTKATVMG NVRVENHTEC HCSTCYYHKS

References

[1]	"Cloning and DNA sequence analysis of the cDNA for the common alpha-subunit of the ovine pituitary glycoprotein hormones." Bello P.A., Mountford P.S., Brandon M.R., Adams T.E. Nucleic Acids Res. 17:10494-10494(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The primary structure of ovine luteinizing hormone. I. The amino acid sequence of the reduced and S-aminoethylated S-subunit (LH-alpha)." Liu W.-K., Nahm H.S., Sweeney C.M., Lamkin W.M., Baker H.N., Ward D.N. J. Biol. Chem. 247:4351-4364(1972) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 25-120.
[3]	"The primary structure of ovine interstitial cell-stimulating hormone. I. The alpha-subunit." Sairam M.R., Papkoff H., Li C.H. Arch. Biochem. Biophys. 153:554-571(1972) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 25-120.
[4]	"Primary structure of the ovine pituitary follitropin alpha-subunit." Sairam M.R. Biochem. J. 197:535-539(1981) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 25-120.
[5]	"Renotropic activity in ovine luteinizing hormone isoform(s)." Nomura K., Tsunasawa S., Ohmura K., Sakiyama F., Shizume K. Endocrinology 123:700-712(1988) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 25-41, FUNCTION, SUBUNIT.
[6]	"The primary structure of ovine interstitial cell-stimulating hormone. III. Disulfide bridges of the alpha-subunit." Chung D., Sairam M.R., Li C.H. Arch. Biochem. Biophys. 159:678-682(1973) Cited for: PRELIMINARY ASSIGNMENT OF DISULFIDE BONDS.
[7]	"Site-specific N-glycosylation of ovine lutropin. Structural analysis by one- and two-dimensional 1H-NMR spectroscopy." Weisshaar G., Hiyama J., Renwick A.G.C. Eur. J. Biochem. 192:741-751(1990) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE OF CARBOHYDRATES.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X16977 mRNA. Translation: CAA34848.1.
PIR	UTSHA. S06935.
RefSeq	NP_001009464.1. NM_001009464.1.
UniGene	Oar.446.
3D structure databases
ProteinModelPortal	P01218.
SMR	P01218. Positions 32-120.
ModBase	Search...
PTM databases
GlycoSuiteDB	P01218.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	443538.
Organism-specific databases
CTD	1081.
Phylogenomic databases
HOVERGEN	HBG000452.
Family and domain databases
InterPro	IPR000476. Glyco_hormone. [Graphical view]
PANTHER	PTHR11509. PTHR11509. 1 hit.
Pfam	PF00236. Hormone_6. 1 hit. [Graphical view]
PRINTS	PR00274. GLYCOHORMONE.
SMART	SM00067. GHA. 1 hit. [Graphical view]
PROSITE	PS00779. GLYCO_HORMONE_ALPHA_1. 1 hit. PS00780. GLYCO_HORMONE_ALPHA_2. 1 hit. PS50277. GLYCO_HORMONE_ALPHA_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLHA_SHEEP

Accession

Primary (citable) accession number: P01218

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	April 1, 1990
Last modified:	April 3, 2013
This is version 84 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program