P01217 (GLHA_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycoprotein hormones alpha chain

Alternative name(s):
Anterior pituitary glycoprotein hormones common subunit alpha
Follicle-stimulating hormone alpha chain
Short name=FSH-alpha
Follitropin alpha chain
Luteinizing hormone alpha chain
Short name=LSH-alpha
Lutropin alpha chain
Thyroid-stimulating hormone alpha chain
Short name=TSH-alpha
Thyrotropin alpha chain

Gene names

Name:

CGA

Organism

Bos taurus (Bovine) [Reference proteome]

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	120 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Subunit structure	Heterodimer of a common alpha chain and a unique beta chain which confers biological specificity to thyrotropin, lutropin, follitropin and gonadotropin.
Subcellular location	Secreted.
Sequence similarities	Belongs to the glycoprotein hormones subunit alpha family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Hormone
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	cellular response to hormone stimulus Inferred from electronic annotation. Source: Compara developmental growth Inferred from electronic annotation. Source: Compara follicle-stimulating hormone secretion Inferred from electronic annotation. Source: Compara gonad development Inferred from electronic annotation. Source: Compara luteinizing hormone secretion Inferred from electronic annotation. Source: Compara negative regulation of organ growth Inferred from electronic annotation. Source: Compara thyroid gland development Inferred from electronic annotation. Source: Compara thyroid hormone generation Inferred from electronic annotation. Source: Compara
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24	Ref.4
	Chain	25 – 120	96	Glycoprotein hormones alpha chain		PRO_0000011633
Amino acid modifications
	Glycosylation	67	1	O-linked (GalNAc...)		CAR_000035
	Glycosylation	80	1	N-linked (GlcNAc...) Ref.4
	Glycosylation	106	1	N-linked (GlcNAc...) Ref.4
	Disulfide bond	35 ↔ 59		By similarity
	Disulfide bond	38 ↔ 88		By similarity
	Disulfide bond	56 ↔ 110		By similarity
	Disulfide bond	60 ↔ 112		By similarity
	Disulfide bond	87 ↔ 115		Ref.7
Experimental info
	Sequence conflict	13	1	A → T in CAA24932. Ref.2
	Sequence conflict	37	1	E → Q AA sequence Ref.4
	Sequence conflict	52	1	P → A in CAA24932. Ref.2
	Sequence conflict	84 – 87	4	EATC → AZCT AA sequence Ref.6

Sequences

	Sequence		Length	Mass (Da)
	P01217 [UniParc]. Last modified October 23, 1986. Version 1. Checksum: 246B415F86D04230	FASTA	120	13,616
	10 20 30 40 50 60 MDYYRKYAAV ILAILSLFLQ ILHSFPDGEF TMQGCPECKL KENKYFSKPD APIYQCMGCC 70 80 90 100 110 120 FSRAYPTPAR SKKTMLVPKN ITSEATCCVA KAFTKATVMG NVRVENHTEC HCSTCYYHKS

References

[1]	"Characterization and nucleotide sequence of the gene for the common alpha subunit of the bovine pituitary glycoprotein hormones." Goodwin R.G., Moncman C.L., Rottman F.M., Nilson J.H. Nucleic Acids Res. 11:6873-6882(1983) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Nucleotide sequence of cloned complementary deoxyribonucleic acid for the alpha subunit of bovine pituitary glycoprotein hormones." Erwin C., Croyle M.L., Donelson J., Maurer R. Biochemistry 22:4856-4860(1983) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Nucleotide sequence of a cDNA for the common alpha subunit of the bovine pituitary glycoprotein hormones. Conservation of nucleotides in the 3'-untranslated region of bovine and human pre-alpha subunit mRNAs." Nilson J.H., Thomason A.R., Cserbak M.T., Moncman C.L., Woychik R.P. J. Biol. Chem. 258:4679-4682(1983) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 8-120.
[4]	"The primary structure of bovine thyrotropin. II. The amino acid sequences of the reduced, S-carboxymethyl alpha and beta chains." Liao T.-H., Pierce J.G. J. Biol. Chem. 246:850-865(1971) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 25-120.
[5]	"Comparisons between the alpha chain of bovine thyrotropin and the CI chain of luteinizing hormone. Compositions of tryptic peptides, cyanogen bromide fragments, and carbohydrate moieties." Pierce J.G., Liao T.-H., Carlsen R.B., Reimo T. J. Biol. Chem. 246:866-872(1971) [PubMed] [Europe PMC] [Abstract] Cited for: PRELIMINARY PARTIAL PROTEIN SEQUENCE.
[6]	"Bovine luteinizing hormone. Study of the primary structure around the carbohydrate attachment sites of the luteinizing hormone alpha-subunit." Maghuin-Rogister G., Hennen G.P. Eur. J. Biochem. 21:489-497(1971) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 80-91 AND 100-120.
[7]	"Studies on the disulfide bonds of glycoprotein hormones. Locations in the alpha chain based on partial reductions and formation of 14C-labeled S-carboxymethyl derivatives." Cornell J.S., Pierce J.G. J. Biol. Chem. 249:4166-4174(1974) [PubMed] [Europe PMC] [Abstract] Cited for: PRELIMINARY ASSIGNMENT OF DISULFIDE BONDS.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X00003, X00004 Genomic DNA. Translation: CAA24907.1. X00050 mRNA. Translation: CAA24932.1.
IPI	IPI00714691.
PIR	TTBOA. A93489.
RefSeq	NP_776326.1. NM_173901.3.
UniGene	Bt.304.
3D structure databases
ProteinModelPortal	P01217.
SMR	P01217. Positions 32-120.
ModBase	Search...
Protein-protein interaction databases
STRING	9913.ENSBTAP00000021481.
PTM databases
GlycoSuiteDB	P01217.
Proteomic databases
PRIDE	P01217.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSBTAT00000021481; ENSBTAP00000021481; ENSBTAG00000016138.
GeneID	280749.
KEGG	bta:280749.
Organism-specific databases
CTD	1081.
Phylogenomic databases
eggNOG	NOG44826.
GeneTree	ENSGT00390000012242.
HOGENOM	HOG000232008.
HOVERGEN	HBG000452.
InParanoid	P01217.
KO	K08522.
OMA	TCCVAKS.
OrthoDB	EOG4G7C11.
Enzyme and pathway databases
BioCyc	CATTLE:280749-MONOMER.
Family and domain databases
InterPro	IPR000476. Glyco_hormone. [Graphical view]
PANTHER	PTHR11509. PTHR11509. 1 hit.
Pfam	PF00236. Hormone_6. 1 hit. [Graphical view]
PRINTS	PR00274. GLYCOHORMONE.
SMART	SM00067. GHA. 1 hit. [Graphical view]
PROSITE	PS00779. GLYCO_HORMONE_ALPHA_1. 1 hit. PS00780. GLYCO_HORMONE_ALPHA_2. 1 hit. PS50277. GLYCO_HORMONE_ALPHA_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20804917.

Entry information

Entry name

GLHA_BOVIN

Accession

Primary (citable) accession number: P01217

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	October 23, 1986
Last modified:	May 29, 2013
This is version 99 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program