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P01215

- GLHA_HUMAN

UniProt

P01215 - GLHA_HUMAN

Protein

Glycoprotein hormones alpha chain

Gene

CGA

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    GO - Molecular functioni

    1. hormone activity Source: ProtInc
    2. protein binding Source: IntAct

    GO - Biological processi

    1. cell-cell signaling Source: ProtInc
    2. cellular protein metabolic process Source: Reactome
    3. cellular response to hormone stimulus Source: Ensembl
    4. developmental growth Source: Ensembl
    5. follicle-stimulating hormone secretion Source: Ensembl
    6. gonad development Source: Ensembl
    7. luteinizing hormone secretion Source: Ensembl
    8. negative regulation of organ growth Source: Ensembl
    9. peptide hormone processing Source: Reactome
    10. positive regulation of cell migration Source: BHF-UCL
    11. positive regulation of cell proliferation Source: BHF-UCL
    12. positive regulation of transcription from RNA polymerase II promoter Source: BHF-UCL
    13. signal transduction Source: ProtInc
    14. thyroid gland development Source: Ensembl
    15. thyroid hormone generation Source: Ensembl

    Keywords - Molecular functioni

    Hormone

    Enzyme and pathway databases

    ReactomeiREACT_11047. Mineralocorticoid biosynthesis.
    REACT_11059. Androgen biosynthesis.
    REACT_15292. Thyroxine biosynthesis.
    REACT_15398. Glycoprotein hormones.
    REACT_16942. Hormone ligand-binding receptors.
    REACT_19327. G alpha (s) signalling events.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glycoprotein hormones alpha chain
    Alternative name(s):
    Anterior pituitary glycoprotein hormones common subunit alpha
    Choriogonadotropin alpha chain
    Chorionic gonadotrophin subunit alpha
    Short name:
    CG-alpha
    Follicle-stimulating hormone alpha chain
    Short name:
    FSH-alpha
    Follitropin alpha chain
    Luteinizing hormone alpha chain
    Short name:
    LSH-alpha
    Lutropin alpha chain
    Thyroid-stimulating hormone alpha chain
    Short name:
    TSH-alpha
    Thyrotropin alpha chain
    Gene namesi
    Name:CGA
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 6

    Organism-specific databases

    HGNCiHGNC:1885. CGA.

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: Reactome

    Keywords - Cellular componenti

    Secreted

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA26433.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 24244 PublicationsAdd
    BLAST
    Chaini25 – 11692Glycoprotein hormones alpha chainPRO_0000011640Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi31 ↔ 552 Publications
    Disulfide bondi34 ↔ 842 Publications
    Disulfide bondi52 ↔ 1062 Publications
    Disulfide bondi56 ↔ 1082 Publications
    Glycosylationi76 – 761N-linked (GlcNAc...)1 PublicationCAR_000036
    Disulfide bondi83 ↔ 1112 Publications
    Glycosylationi102 – 1021N-linked (GlcNAc...)1 PublicationCAR_000037

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    MaxQBiP01215.
    PaxDbiP01215.
    PRIDEiP01215.

    PTM databases

    PhosphoSiteiP01215.
    UniCarbKBiP01215.

    Expressioni

    Gene expression databases

    ArrayExpressiP01215.
    BgeeiP01215.
    CleanExiHS_CGA.
    GenevestigatoriP01215.

    Organism-specific databases

    HPAiCAB023350.
    HPA029698.

    Interactioni

    Subunit structurei

    Heterodimer of a common alpha chain and a unique beta chain which confers biological specificity to thyrotropin, lutropin, follitropin and gonadotropin.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    FSHBP012252EBI-718913,EBI-1030645

    Protein-protein interaction databases

    BioGridi107507. 5 interactions.
    DIPiDIP-6182N.
    IntActiP01215. 3 interactions.
    MINTiMINT-1197885.
    STRINGi9606.ENSP00000358595.

    Structurei

    Secondary structure

    1
    116
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi33 – 386
    Turni40 – 423
    Beta strandi50 – 6213
    Helixi65 – 684
    Beta strandi76 – 805
    Beta strandi83 – 9412
    Turni95 – 973
    Beta strandi98 – 10912
    Beta strandi112 – 1143

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1DZ7NMR-A25-116[»]
    1E9JNMR-A25-116[»]
    1FL7X-ray3.00A/C25-116[»]
    1HCNX-ray2.60A25-116[»]
    1HD4NMR-A25-116[»]
    1HRPX-ray3.00A25-116[»]
    1QFWX-ray3.50A25-116[»]
    1XULmodel-A25-116[»]
    1XWDX-ray2.92A/D25-116[»]
    4AY9X-ray2.50A/D/G25-116[»]
    4MQWX-ray2.90A/D/G25-116[»]
    ProteinModelPortaliP01215.
    SMRiP01215. Positions 27-116.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP01215.

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG44826.
    HOGENOMiHOG000232008.
    HOVERGENiHBG000452.
    InParanoidiP01215.
    KOiK08522.
    OMAiDGEFTMQ.
    OrthoDBiEOG73FQPJ.
    PhylomeDBiP01215.
    TreeFamiTF332733.

    Family and domain databases

    Gene3Di2.10.90.10. 1 hit.
    InterProiIPR029034. Cystine-knot_cytokine.
    IPR000476. Glyco_hormone.
    [Graphical view]
    PANTHERiPTHR11509. PTHR11509. 1 hit.
    PfamiPF00236. Hormone_6. 1 hit.
    [Graphical view]
    PRINTSiPR00274. GLYCOHORMONE.
    SMARTiSM00067. GHA. 1 hit.
    [Graphical view]
    SUPFAMiSSF57501. SSF57501. 1 hit.
    PROSITEiPS00779. GLYCO_HORMONE_ALPHA_1. 1 hit.
    PS00780. GLYCO_HORMONE_ALPHA_2. 1 hit.
    PS50277. GLYCO_HORMONE_ALPHA_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P01215-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDYYRKYAAI FLVTLSVFLH VLHSAPDVQD CPECTLQENP FFSQPGAPIL    50
    QCMGCCFSRA YPTPLRSKKT MLVQKNVTSE STCCVAKSYN RVTVMGGFKV 100
    ENHTACHCST CYYHKS 116
    Length:116
    Mass (Da):13,075
    Last modified:July 21, 1986 - v1
    Checksum:iF0623CD8CC90CFCD
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti29 – 291Q → E AA sequence (PubMed:1158880)Curated
    Sequence conflicti108 – 1092CS → SC AA sequence (PubMed:890569)Curated
    Sequence conflicti108 – 1092CS → SC AA sequence (PubMed:5065401)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J00152, J00150, J00151 Genomic DNA. Translation: AAD13690.1.
    S70585, S70583, S70584 Genomic DNA. Translation: AAB30827.1.
    BC020782 mRNA. Translation: AAH20782.1.
    BC055080 mRNA. Translation: AAH55080.1.
    V00518 mRNA. Translation: CAA23777.1.
    CCDSiCCDS5007.1.
    PIRiA93213. TTHUAP.
    RefSeqiNP_000726.1. NM_000735.3.
    NP_001239312.1. NM_001252383.1.
    UniGeneiHs.119689.

    Genome annotation databases

    EnsembliENST00000369582; ENSP00000358595; ENSG00000135346.
    GeneIDi1081.
    KEGGihsa:1081.
    UCSCiuc003plj.2. human.

    Polymorphism databases

    DMDMi121312.

    Cross-referencesi

    Web resourcesi

    Protein Spotlight

    Proteic grace - Issue 77 of December 2006

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J00152 , J00150 , J00151 Genomic DNA. Translation: AAD13690.1 .
    S70585 , S70583 , S70584 Genomic DNA. Translation: AAB30827.1 .
    BC020782 mRNA. Translation: AAH20782.1 .
    BC055080 mRNA. Translation: AAH55080.1 .
    V00518 mRNA. Translation: CAA23777.1 .
    CCDSi CCDS5007.1.
    PIRi A93213. TTHUAP.
    RefSeqi NP_000726.1. NM_000735.3.
    NP_001239312.1. NM_001252383.1.
    UniGenei Hs.119689.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1DZ7 NMR - A 25-116 [» ]
    1E9J NMR - A 25-116 [» ]
    1FL7 X-ray 3.00 A/C 25-116 [» ]
    1HCN X-ray 2.60 A 25-116 [» ]
    1HD4 NMR - A 25-116 [» ]
    1HRP X-ray 3.00 A 25-116 [» ]
    1QFW X-ray 3.50 A 25-116 [» ]
    1XUL model - A 25-116 [» ]
    1XWD X-ray 2.92 A/D 25-116 [» ]
    4AY9 X-ray 2.50 A/D/G 25-116 [» ]
    4MQW X-ray 2.90 A/D/G 25-116 [» ]
    ProteinModelPortali P01215.
    SMRi P01215. Positions 27-116.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 107507. 5 interactions.
    DIPi DIP-6182N.
    IntActi P01215. 3 interactions.
    MINTi MINT-1197885.
    STRINGi 9606.ENSP00000358595.

    Chemistry

    ChEMBLi CHEMBL2146305.

    PTM databases

    PhosphoSitei P01215.
    UniCarbKBi P01215.

    Polymorphism databases

    DMDMi 121312.

    Proteomic databases

    MaxQBi P01215.
    PaxDbi P01215.
    PRIDEi P01215.

    Protocols and materials databases

    DNASUi 1081.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000369582 ; ENSP00000358595 ; ENSG00000135346 .
    GeneIDi 1081.
    KEGGi hsa:1081.
    UCSCi uc003plj.2. human.

    Organism-specific databases

    CTDi 1081.
    GeneCardsi GC06M087795.
    HGNCi HGNC:1885. CGA.
    HPAi CAB023350.
    HPA029698.
    MIMi 118850. gene.
    neXtProti NX_P01215.
    PharmGKBi PA26433.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG44826.
    HOGENOMi HOG000232008.
    HOVERGENi HBG000452.
    InParanoidi P01215.
    KOi K08522.
    OMAi DGEFTMQ.
    OrthoDBi EOG73FQPJ.
    PhylomeDBi P01215.
    TreeFami TF332733.

    Enzyme and pathway databases

    Reactomei REACT_11047. Mineralocorticoid biosynthesis.
    REACT_11059. Androgen biosynthesis.
    REACT_15292. Thyroxine biosynthesis.
    REACT_15398. Glycoprotein hormones.
    REACT_16942. Hormone ligand-binding receptors.
    REACT_19327. G alpha (s) signalling events.

    Miscellaneous databases

    ChiTaRSi CGA. human.
    EvolutionaryTracei P01215.
    GeneWikii Chorionic_gonadotropin_alpha.
    GenomeRNAii 1081.
    NextBioi 4504.
    PROi P01215.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P01215.
    Bgeei P01215.
    CleanExi HS_CGA.
    Genevestigatori P01215.

    Family and domain databases

    Gene3Di 2.10.90.10. 1 hit.
    InterProi IPR029034. Cystine-knot_cytokine.
    IPR000476. Glyco_hormone.
    [Graphical view ]
    PANTHERi PTHR11509. PTHR11509. 1 hit.
    Pfami PF00236. Hormone_6. 1 hit.
    [Graphical view ]
    PRINTSi PR00274. GLYCOHORMONE.
    SMARTi SM00067. GHA. 1 hit.
    [Graphical view ]
    SUPFAMi SSF57501. SSF57501. 1 hit.
    PROSITEi PS00779. GLYCO_HORMONE_ALPHA_1. 1 hit.
    PS00780. GLYCO_HORMONE_ALPHA_2. 1 hit.
    PS50277. GLYCO_HORMONE_ALPHA_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation, cloning and sequence analysis of the cDNA for the alpha-subunit of human chorionic gonadotropin."
      Fiddes J.C., Goodman H.M.
      Nature 281:351-356(1979) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Structure and regulation of human thyroid-stimulating hormone (TSH) gene."
      Miyoshi I., Kasai N., Hayashizaki Y.
      Nippon Rinsho 52:940-947(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Placenta.
    4. "The gene encoding the common alpha subunit of the four human glycoprotein hormones."
      Fiddes J.C., Goodman H.M.
      J. Mol. Appl. Genet. 1:3-18(1981) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-98.
    5. "The amino acid sequences of the prepeptides contained in the alpha and beta subunits of human choriogonadotropin."
      Birken S., Fetherston J., Canfield R.E., Boime I.
      J. Biol. Chem. 256:1816-1823(1981) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 1-24.
    6. "Human pituitary thyrotropin. The primary structure of the alpha and beta subunits."
      Sairam M.R., Li C.H.
      Can. J. Biochem. 55:755-760(1977) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 28-116.
    7. "Human pituitary interstitial cell stimulating hormone: primary structure of the alpha-subunit."
      Sairam M.R., Papkoff H., Li C.H.
      Biochem. Biophys. Res. Commun. 48:530-537(1972) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 28-116.
    8. "Structure of human luteninizing hormone."
      Keutmann H.T., Williams R.M., Bishop W.H., Ryan R.J.
      Fed. Proc. 37:1828-1828(1978)
      Cited for: PROTEIN SEQUENCE, SEQUENCE REVISION.
    9. "Primary amino acid sequence of follicle-stimulating hormone from human pituitary glands. I. alpha subunit."
      Rathnam P., Saxena B.B.
      J. Biol. Chem. 250:6735-6746(1975) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 25-116.
      Tissue: Pituitary.
    10. "Human follicle stimulating hormone (hFSH): first proposal for the amino acid sequence of the alpha-subunit (hFSHa) and first demonstration of its identity with the alpha-subunit of human luteinizing hormone (hLHa)."
      Shome B., Parlow A.F.
      J. Clin. Endocrinol. Metab. 39:199-202(1974) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 28-116.
    11. "The amino acid sequence of human chorionic gonadotropin. The alpha subunit and beta subunit."
      Morgan F.J., Birken S., Canfield R.E.
      J. Biol. Chem. 250:5247-5258(1975) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 25-116.
    12. "Human chorionic gonadotropin. Linear amino acid sequence of the alpha subunit."
      Bellisario R., Carlsen R.B., Bahl O.P.
      J. Biol. Chem. 248:6796-6809(1973) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 25-116.
    13. "Studies on the disulfide bonds in human pituitary follicle-stimulating hormone."
      Fujiki Y., Rathnam P., Saxena B.B.
      Biochim. Biophys. Acta 624:428-435(1980) [PubMed] [Europe PMC] [Abstract]
      Cited for: PRELIMINARY ASSIGNMENT OF DISULFIDE BONDS.
    14. "Assignment of disulfide bonds in the alpha subunit of human chorionic gonadotropin."
      Mise T., Bahl O.P.
      J. Biol. Chem. 255:8516-8522(1980) [PubMed] [Europe PMC] [Abstract]
      Cited for: PRELIMINARY ASSIGNMENT OF DISULFIDE BONDS.
    15. "NMR investigations of the N-linked oligosaccharides at individual glycosylation sites of human lutropin."
      Weisshaar G., Hiyama J., Renwick A.G.C., Nimtz M.
      Eur. J. Biochem. 195:257-268(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE OF CARBOHYDRATES.
    16. Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS).
    17. "NMR studies of the free alpha subunit of human chorionic gonadotropin. Structural influences of N-glycosylation and the beta subunit on the conformation of the alpha subunit."
      de Beer T., van Zuylen C.W.E.M., Leeflang B.R., Haard K., Boelens R., Kaptein R., Kamerling J.P., Vliegenthart J.F.G.
      Eur. J. Biochem. 241:229-242(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR.
    18. "Structure of human follicle-stimulating hormone in complex with its receptor."
      Fan Q.R., Hendrickson W.A.
      Nature 433:269-277(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.92 ANGSTROMS) OF 25-116 IN COMPLEX WITH FSHB AND FSHR, DISULFIDE BONDS, GLYCOSYLATION AT ASN-76 AND ASN-102.

    Entry informationi

    Entry nameiGLHA_HUMAN
    AccessioniPrimary (citable) accession number: P01215
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: July 21, 1986
    Last modified: October 1, 2014
    This is version 156 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human chromosome 6
      Human chromosome 6: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. Protein Spotlight
      Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3