ID   PDYN_PIG                Reviewed;         256 AA.
AC   P01214;
DT   21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT   21-JUL-1986, sequence version 1.
DT   24-JAN-2024, entry version 106.
DE   RecName: Full=Proenkephalin-B;
DE   AltName: Full=Beta-neoendorphin-dynorphin;
DE   AltName: Full=Preprodynorphin;
DE   Contains:
DE     RecName: Full=Alpha-neoendorphin;
DE   Contains:
DE     RecName: Full=Beta-neoendorphin;
DE   Contains:
DE     RecName: Full=Big dynorphin;
DE              Short=Big Dyn;
DE   Contains:
DE     RecName: Full=Dynorphin A(1-17);
DE              Short=Dyn-A17;
DE              Short=Dynorphin A;
DE   Contains:
DE     RecName: Full=Dynorphin A(1-13);
DE   Contains:
DE     RecName: Full=Dynorphin A(1-8);
DE   Contains:
DE     RecName: Full=Leu-enkephalin;
DE   Contains:
DE     RecName: Full=Rimorphin;
DE     AltName: Full=Dynorphin B;
DE              Short=Dyn-B;
DE     AltName: Full=Dynorphin B(1-13);
DE   Contains:
DE     RecName: Full=Leumorphin;
DE     AltName: Full=Dynorphin B-29;
DE   Flags: Precursor;
GN   Name=PDYN;
OS   Sus scrofa (Pig).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Suina; Suidae; Sus.
OX   NCBI_TaxID=9823;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Hypothalamus;
RX   PubMed=6123953; DOI=10.1038/298245a0;
RA   Kakidani H., Furutani Y., Takahashi H., Noda M., Morimoto Y., Hirose T.,
RA   Asai M., Inayama S., Nakanishi S., Numa S.;
RT   "Cloning and sequence analysis of cDNA for porcine beta-neo-
RT   endorphin/dynorphin precursor.";
RL   Nature 298:245-249(1982).
RN   [2]
RP   PROTEIN SEQUENCE OF 209-221, IDENTIFICATION, AND CHARACTERIZATION OF
RP   DYNORPHIN A(1-13).
RX   PubMed=230519; DOI=10.1073/pnas.76.12.6666;
RA   Goldstein A., Tachibana S., Lowney L.I., Hunkapiller M., Hood L.;
RT   "Dynorphin-(1-13), an extraordinarily potent opioid peptide.";
RL   Proc. Natl. Acad. Sci. U.S.A. 76:6666-6670(1979).
RN   [3]
RP   PROTEIN SEQUENCE OF 209-216, AND IDENTIFICATION OF DYNORPHIN A(1-8).
RC   TISSUE=Hypothalamus;
RX   PubMed=7191256; DOI=10.1016/s0006-291x(80)80063-5;
RA   Minamino N., Kangawa K., Fukuda A., Matsuo H., Iagarashi M.;
RT   "A new opioid octapeptide related to dynorphin from porcine hypothalamus.";
RL   Biochem. Biophys. Res. Commun. 95:1475-1481(1980).
RN   [4]
RP   IDENTIFICATION OF LEUMORPHIN AND RIMORPHIN.
RX   PubMed=6689399; DOI=10.1016/0006-291x(83)91653-4;
RA   Nakao K., Suda M., Sakamoto M., Yoshimasa T., Morii N., Ikeda Y.,
RA   Yanaihara C., Yanaihara N., Numa S., Imura H.;
RT   "Leumorphin is a novel endogenous opioid peptide derived from
RT   preproenkephalin B.";
RL   Biochem. Biophys. Res. Commun. 117:695-701(1983).
CC   -!- FUNCTION: Leu-enkephalins compete with and mimic the effects of opiate
CC       drugs. They play a role in a number of physiologic functions, including
CC       pain perception and responses to stress (By similarity). {ECO:0000250}.
CC   -!- FUNCTION: Dynorphin peptides differentially regulate the kappa opioid
CC       receptor. Dynorphin A(1-13) has a typical opioid activity, it is 700
CC       times more potent than Leu-enkephalin.
CC   -!- FUNCTION: Leumorphin has a typical opioid activity and may have anti-
CC       apoptotic effect. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- PTM: The N-terminal domain contains 6 conserved cysteines thought to be
CC       involved in disulfide bonding and/or processing.
CC   -!- SIMILARITY: Belongs to the opioid neuropeptide precursor family.
CC       {ECO:0000305}.
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DR   EMBL; V01362; CAA24650.1; -; mRNA.
DR   PIR; A93280; DFPG.
DR   RefSeq; NP_001004040.1; NM_001004040.1.
DR   AlphaFoldDB; P01214; -.
DR   BMRB; P01214; -.
DR   STRING; 9823.ENSSSCP00000031176; -.
DR   PaxDb; 9823-ENSSSCP00000007643; -.
DR   GeneID; 445529; -.
DR   KEGG; ssc:445529; -.
DR   CTD; 5173; -.
DR   eggNOG; ENOG502RXT4; Eukaryota.
DR   InParanoid; P01214; -.
DR   OrthoDB; 5318835at2759; -.
DR   Proteomes; UP000008227; Unplaced.
DR   Proteomes; UP000314985; Unplaced.
DR   Proteomes; UP000694570; Unplaced.
DR   Proteomes; UP000694571; Unplaced.
DR   Proteomes; UP000694720; Unplaced.
DR   Proteomes; UP000694722; Unplaced.
DR   Proteomes; UP000694723; Unplaced.
DR   Proteomes; UP000694724; Unplaced.
DR   Proteomes; UP000694725; Unplaced.
DR   Proteomes; UP000694726; Unplaced.
DR   Proteomes; UP000694727; Unplaced.
DR   Proteomes; UP000694728; Unplaced.
DR   GO; GO:0043679; C:axon terminus; IBA:GO_Central.
DR   GO; GO:0030425; C:dendrite; IBA:GO_Central.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0043025; C:neuronal cell body; IBA:GO_Central.
DR   GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR   GO; GO:0001515; F:opioid peptide activity; IEA:UniProtKB-KW.
DR   GO; GO:0031628; F:opioid receptor binding; IBA:GO_Central.
DR   GO; GO:0007268; P:chemical synaptic transmission; IBA:GO_Central.
DR   GO; GO:0007218; P:neuropeptide signaling pathway; IBA:GO_Central.
DR   GO; GO:0007600; P:sensory perception; IBA:GO_Central.
DR   InterPro; IPR006024; Opioid_neupept.
DR   InterPro; IPR000750; Proenkphlin_B.
DR   PANTHER; PTHR11438; PROENKEPHALIN; 1.
DR   PANTHER; PTHR11438:SF4; PROENKEPHALIN-B; 1.
DR   Pfam; PF01160; Opiods_neuropep; 1.
DR   PRINTS; PR01028; OPIOIDPRCRSR.
DR   PRINTS; PR01030; PENKBPRCRSR.
DR   PROSITE; PS01252; OPIOIDS_PRECURSOR; 1.
PE   1: Evidence at protein level;
KW   Cleavage on pair of basic residues; Direct protein sequencing;
KW   Disulfide bond; Endorphin; Neuropeptide; Neurotransmitter; Opioid peptide;
KW   Reference proteome; Secreted; Signal.
FT   SIGNAL          1..20
FT                   /evidence="ECO:0000255"
FT   PROPEP          21..172
FT                   /id="PRO_0000008191"
FT   PEPTIDE         175..184
FT                   /note="Alpha-neoendorphin"
FT                   /evidence="ECO:0000250"
FT                   /id="PRO_0000306358"
FT   PEPTIDE         175..183
FT                   /note="Beta-neoendorphin"
FT                   /id="PRO_0000008192"
FT   PEPTIDE         175..179
FT                   /note="Leu-enkephalin"
FT                   /evidence="ECO:0000250"
FT                   /id="PRO_0000306359"
FT   PROPEP          186..206
FT                   /id="PRO_0000008193"
FT   PEPTIDE         209..240
FT                   /note="Big dynorphin"
FT                   /evidence="ECO:0000250"
FT                   /id="PRO_0000306360"
FT   PEPTIDE         209..225
FT                   /note="Dynorphin A(1-17)"
FT                   /id="PRO_0000306361"
FT   PEPTIDE         209..221
FT                   /note="Dynorphin A(1-13)"
FT                   /id="PRO_0000306362"
FT   PEPTIDE         209..216
FT                   /note="Dynorphin A(1-8)"
FT                   /id="PRO_0000306363"
FT   PEPTIDE         209..213
FT                   /note="Leu-enkephalin"
FT                   /evidence="ECO:0000250"
FT                   /id="PRO_0000306364"
FT   PEPTIDE         228..256
FT                   /note="Leumorphin"
FT                   /id="PRO_0000008195"
FT   PEPTIDE         228..240
FT                   /note="Rimorphin"
FT                   /id="PRO_0000008196"
FT   PEPTIDE         228..232
FT                   /note="Leu-enkephalin"
FT                   /id="PRO_0000008197"
FT   VARIANT         147
FT                   /note="G -> E"
FT   VARIANT         197
FT                   /note="R -> G"
SQ   SEQUENCE   256 AA;  28617 MW;  6814412E3A8DB3AE CRC64;
     MAWQGLLLAA CLLVLPSTMA DCLSGCSLCA VKTQDGPKPI NPLICSLECQ AALQPAEEWE
     RCQGLLSFLA PLSLGLEGKE DLESKAALEE PSSELVKYMG PFLKELEKNR FLLSTPAEET
     SLSRSLVEKL RSLPGRLGEE TESELMGDAQ QNDGAMEAAA LDSSVEDPKE QVKRYGGFLR
     KYPKRSSEVA GEGDGDRDKV GHEDLYKRYG GFLRRIRPKL KWDNQKRYGG FLRRQFKVVT
     RSQEDPNAYY EELFDV
//