P01212 (PENKA_XENLA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Proenkephalin-A-A

Cleaved into the following 4 chains:

1. Synenkephalin
2. Met-enkephalin
3. Met-enkephalin-Arg-Gly-Tyr
4. Met-enkephalin-Arg-Phe

Gene names

Name:

penk-a

Organism

Xenopus laevis (African clawed frog)

Taxonomic identifier

8355 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amphibia › Batrachia › Anura › Pipoidea › Pipidae › Xenopodinae › Xenopus › Xenopus

Protein attributes

Sequence length	262 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Subcellular location	Secreted.
Post-translational modification	The N-terminal domain contains 6 conserved cysteines thought to be involved in disulfide bonding and/or processing.
Sequence similarities	Belongs to the opioid neuropeptide precursor family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Endorphin Neuropeptide Opioid peptide
PTM	Cleavage on pair of basic residues Disulfide bond
Gene Ontology (GO)
Biological_process	neuropeptide signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24	Potential
	Peptide	25 – 93	69	Synenkephalin		PRO_0000008294
	Peptide	96 – 100	5	Met-enkephalin		PRO_0000008295
	Peptide	103 – 107	5	Met-enkephalin		PRO_0000008296
	Propeptide	110 – 131	22			PRO_0000008297
	Peptide	132 – 136	5	Met-enkephalin		PRO_0000008298
	Propeptide	139 – 177	39			PRO_0000008299
	Peptide	180 – 187	8	Met-enkephalin-Arg-Gly-Tyr		PRO_0000008300
	Propeptide	190 – 201	12			PRO_0000008301
	Peptide	204 – 208	5	Met-enkephalin		PRO_0000008302
	Propeptide	211 – 221	11			PRO_0000008303
	Peptide	224 – 228	5	Met-enkephalin		PRO_0000008304
	Propeptide	229 – 253	25			PRO_0000008305
	Peptide	256 – 262	7	Met-enkephalin-Arg-Phe		PRO_0000008306
Amino acid modifications
	Disulfide bond	26 ↔ 48		By similarity
	Disulfide bond	30 ↔ 52		By similarity
	Disulfide bond	33 ↔ 65		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P01212 [UniParc]. Last modified October 1, 1996. Version 2. Checksum: 5B51C6133218B072	FASTA	262	29,932
	10 20 30 40 50 60 MGLEARHCCM FLLVFASLSV EIRADCSKDC ASCALHLGQQ REINSLACTL ECEGKLPSAK 70 80 90 100 110 120 AWGTCKELLL LTKVDNVQDG EKYQDNNDSH YAAKKYGGFM KRYGGFMKKM DELYHAEPEE 130 140 150 160 170 180 DDAGGEILAK NYGGFMKKEY DSDRDAADLL RELLATSGDP ESSIYHDNNS ETPGEINKRY 190 200 210 220 230 240 GGFMRGYRRS TDLEDETSGI QKRYGGFMRR VGRPEWWEDY QKRYGGFMTR FTDSFLPSDE 250 260 DGESYSKENP DMEKRYGGFM RF

References

[1]	"Characterization of Xenopus laevis proenkephalin gene." Wong M., Rius R.A., Loh Y.P. Brain Res. Mol. Brain Res. 11:197-205(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Polymorphism and absence of Leu-enkephalin sequences in proenkephalin genes in Xenopus laevis." Martens G.J.M., Herbert E. Nature 310:251-254(1984) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 47-262.

Cross-references

Sequence databases
EMBL GenBank DDBJ	S72932, S72930 Genomic DNA. Translation: AAB20686.1. X00852 Genomic DNA. Translation: CAA25405.1.
PIR	EQXL. A43949.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
HOVERGEN	HBG000063.
Family and domain databases
InterPro	IPR006024. Opioid_neupept. IPR000703. Proenkphlin_A. [Graphical view]
PANTHER	PTHR11438. PTHR11438. 1 hit. PTHR11438:SF3. PTHR11438:SF3. 1 hit.
Pfam	PF01160. Opiods_neuropep. 1 hit. [Graphical view]
PRINTS	PR01028. OPIOIDPRCRSR. PR01029. PENKAPRCRSR.
PROSITE	PS01252. OPIOIDS_PRECURSOR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PENKA_XENLA

Accession

Primary (citable) accession number: P01212

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	October 1, 1996
Last modified:	April 3, 2013
This is version 61 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program