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Protein

Proenkephalin-A

Gene

PENK

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Met- and Leu-enkephalins compete with and mimic the effects of opiate drugs. They play a role in a number of physiologic functions, including pain perception and responses to stress. PENK(111-130) and PENK(233-254) increase glutamate release in the striatum. PENK(111-130) decreases GABA concentration in the striatum.
Enkelytin possesses antibacterial activity against Gram-positive bacteria such as Micrococcus luteus and Bacillus megaterium.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Antibiotic, Antimicrobial, Endorphin, Neuropeptide, Opioid peptide

Enzyme and pathway databases

ReactomeiR-BTA-375276. Peptide ligand-binding receptors.
R-BTA-418594. G alpha (i) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
Proenkephalin-A
Cleaved into the following 9 chains:
Gene namesi
Name:PENK
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 14

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24Sequence analysisAdd BLAST24
PeptideiPRO_000000820625 – 94SynenkephalinAdd BLAST70
PeptideiPRO_000000820797 – 101Met-enkephalin5
PeptideiPRO_0000008208104 – 108Met-enkephalin5
PeptideiPRO_0000377682111 – 130PENK(111-130)By similarityAdd BLAST20
PeptideiPRO_0000008210133 – 137Met-enkephalin5
PeptideiPRO_0000377683140 – 179PENK(140-179)By similarityAdd BLAST40
PeptideiPRO_0000008212182 – 189Met-enkephalin-Arg-Gly-Leu8
PropeptideiPRO_0000008213192 – 203Add BLAST12
PeptideiPRO_0000008214206 – 210Met-enkephalin5
PropeptideiPRO_0000008215213 – 223Add BLAST11
PeptideiPRO_0000008216226 – 230Leu-enkephalin5
PeptideiPRO_0000008217233 – 261EnkelytinAdd BLAST29
PeptideiPRO_0000377684233 – 254PENK(233-254)By similarityAdd BLAST22
PeptideiPRO_0000008218257 – 263Met-enkephalin-Arg-Phe7

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi26 ↔ 48By similarity
Disulfide bondi30 ↔ 52By similarity
Disulfide bondi33 ↔ 65By similarity
Modified residuei247PhosphoserineBy similarity1

Post-translational modificationi

The N-terminal domain contains 6 conserved cysteines thought to be involved in disulfide bonding and/or processing.

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Phosphoprotein

Proteomic databases

PaxDbiP01211.
PRIDEiP01211.

PTM databases

iPTMnetiP01211.

Expressioni

Gene expression databases

BgeeiENSBTAG00000004924.

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000006478.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IJUY. Eukaryota.
ENOG4110ZYD. LUCA.
GeneTreeiENSGT00530000063761.
HOGENOMiHOG000013003.
HOVERGENiHBG000063.
InParanoidiP01211.
KOiK18832.
OMAiFMKKMDE.
OrthoDBiEOG091G0HV8.
TreeFamiTF332620.

Family and domain databases

InterProiIPR006024. Opioid_neupept.
IPR000703. Proenkphlin_A.
[Graphical view]
PANTHERiPTHR11438. PTHR11438. 1 hit.
PTHR11438:SF3. PTHR11438:SF3. 1 hit.
PfamiPF01160. Opiods_neuropep. 1 hit.
[Graphical view]
PRINTSiPR01028. OPIOIDPRCRSR.
PR01029. PENKAPRCRSR.
PROSITEiPS01252. OPIOIDS_PRECURSOR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P01211-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARFLGLCTW LLALGPGLLA TVRAECSQDC ATCSYRLARP TDLNPLACTL
60 70 80 90 100
ECEGKLPSLK TWETCKELLQ LTKLELPPDA TSALSKQEES HLLAKKYGGF
110 120 130 140 150
MKRYGGFMKK MDELYPLEVE EEANGGEVLG KRYGGFMKKD AEEDDGLGNS
160 170 180 190 200
SNLLKELLGA GDQREGSLHQ EGSDAEDVSK RYGGFMRGLK RSPHLEDETK
210 220 230 240 250
ELQKRYGGFM RRVGRPEWWM DYQKRYGGFL KRFAEPLPSE EEGESYSKEV
260
PEMEKRYGGF MRF
Length:263
Mass (Da):29,788
Last modified:July 21, 1986 - v1
Checksum:i0E400338A228B0D8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V00109 mRNA. Translation: CAA23443.1.
BC111279 mRNA. Translation: AAI11280.1.
V00108 mRNA. Translation: CAA23442.1.
J00012 mRNA. Translation: AAA30673.1.
PIRiA93272. EQBOA.
RefSeqiNP_776566.1. NM_174141.2.
UniGeneiBt.166.

Genome annotation databases

EnsembliENSBTAT00000006478; ENSBTAP00000006478; ENSBTAG00000004924.
GeneIDi281387.
KEGGibta:281387.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V00109 mRNA. Translation: CAA23443.1.
BC111279 mRNA. Translation: AAI11280.1.
V00108 mRNA. Translation: CAA23442.1.
J00012 mRNA. Translation: AAA30673.1.
PIRiA93272. EQBOA.
RefSeqiNP_776566.1. NM_174141.2.
UniGeneiBt.166.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000006478.

PTM databases

iPTMnetiP01211.

Proteomic databases

PaxDbiP01211.
PRIDEiP01211.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000006478; ENSBTAP00000006478; ENSBTAG00000004924.
GeneIDi281387.
KEGGibta:281387.

Organism-specific databases

CTDi5179.

Phylogenomic databases

eggNOGiENOG410IJUY. Eukaryota.
ENOG4110ZYD. LUCA.
GeneTreeiENSGT00530000063761.
HOGENOMiHOG000013003.
HOVERGENiHBG000063.
InParanoidiP01211.
KOiK18832.
OMAiFMKKMDE.
OrthoDBiEOG091G0HV8.
TreeFamiTF332620.

Enzyme and pathway databases

ReactomeiR-BTA-375276. Peptide ligand-binding receptors.
R-BTA-418594. G alpha (i) signalling events.

Gene expression databases

BgeeiENSBTAG00000004924.

Family and domain databases

InterProiIPR006024. Opioid_neupept.
IPR000703. Proenkphlin_A.
[Graphical view]
PANTHERiPTHR11438. PTHR11438. 1 hit.
PTHR11438:SF3. PTHR11438:SF3. 1 hit.
PfamiPF01160. Opiods_neuropep. 1 hit.
[Graphical view]
PRINTSiPR01028. OPIOIDPRCRSR.
PR01029. PENKAPRCRSR.
PROSITEiPS01252. OPIOIDS_PRECURSOR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPENK_BOVIN
AccessioniPrimary (citable) accession number: P01211
Secondary accession number(s): Q2T9T4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: September 7, 2016
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.