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P01211 (PENK_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 97. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Proenkephalin-A
Gene names
Name:PENK
OrganismBos taurus (Bovine) [Reference proteome]
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos

Protein attributes

Sequence length263 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Met- and Leu-enkephalins compete with and mimic the effects of opiate drugs. They play a role in a number of physiologic functions, including pain perception and responses to stress. PENK(111-130) and PENK(233-254) increase glutamate release in the striatum. PENK(111-130) decreases GABA concentration in the striatum.

Enkelytin possesses antibacterial activity against Gram-positive bacteria such as Micrococcus luteus and Bacillus megaterium.

Subcellular location

Secreted.

Post-translational modification

The N-terminal domain contains 6 conserved cysteines thought to be involved in disulfide bonding and/or processing.

Sequence similarities

Belongs to the opioid neuropeptide precursor family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2424 Potential
Peptide25 – 9470Synenkephalin
PRO_0000008206
Peptide97 – 1015Met-enkephalin
PRO_0000008207
Peptide104 – 1085Met-enkephalin
PRO_0000008208
Peptide111 – 13020PENK(111-130) By similarity
PRO_0000377682
Peptide133 – 1375Met-enkephalin
PRO_0000008210
Peptide140 – 17940PENK(140-179) By similarity
PRO_0000377683
Peptide182 – 1898Met-enkephalin-Arg-Gly-Leu
PRO_0000008212
Propeptide192 – 20312
PRO_0000008213
Peptide206 – 2105Met-enkephalin
PRO_0000008214
Propeptide213 – 22311
PRO_0000008215
Peptide226 – 2305Leu-enkephalin
PRO_0000008216
Peptide233 – 26129Enkelytin Ref.5
PRO_0000008217
Peptide233 – 25422PENK(233-254) By similarity
PRO_0000377684
Peptide257 – 2637Met-enkephalin-Arg-Phe
PRO_0000008218

Amino acid modifications

Disulfide bond26 ↔ 48 By similarity
Disulfide bond30 ↔ 52 By similarity
Disulfide bond33 ↔ 65 By similarity

Sequences

Sequence LengthMass (Da)Tools
P01211 [UniParc].

Last modified July 21, 1986. Version 1.
Checksum: 0E400338A228B0D8

FASTA26329,788
        10         20         30         40         50         60 
MARFLGLCTW LLALGPGLLA TVRAECSQDC ATCSYRLARP TDLNPLACTL ECEGKLPSLK 

        70         80         90        100        110        120 
TWETCKELLQ LTKLELPPDA TSALSKQEES HLLAKKYGGF MKRYGGFMKK MDELYPLEVE 

       130        140        150        160        170        180 
EEANGGEVLG KRYGGFMKKD AEEDDGLGNS SNLLKELLGA GDQREGSLHQ EGSDAEDVSK 

       190        200        210        220        230        240 
RYGGFMRGLK RSPHLEDETK ELQKRYGGFM RRVGRPEWWM DYQKRYGGFL KRFAEPLPSE 

       250        260 
EEGESYSKEV PEMEKRYGGF MRF 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and sequence analysis of cDNA for bovine adrenal preproenkephalin."
Noda M., Furutani Y., Takahashi H., Toyosato M., Hirose T., Inayama S., Nakanishi S., Numa S.
Nature 295:202-206(1982) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]NIH - Mammalian Gene Collection (MGC) project
Submitted (DEC-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Crossbred X Angus.
Tissue: Liver.
[3]"Molecular cloning establishes proenkephalin as precursor of enkephalin-containing peptides."
Gubler U., Seeburg P., Hoffman B.J., Gage L.P., Udenfriend S.
Nature 295:206-208(1982) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 47-263.
[4]"Partial characterization of the mRNA that codes for enkephalins in bovine adrenal medulla and human pheochromocytoma."
Comb M., Herbert E., Crea R.
Proc. Natl. Acad. Sci. U.S.A. 79:360-364(1982) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 211-229.
[5]"The C-terminal bisphosphorylated proenkephalin-A-(209-237)-peptide from adrenal medullary chromaffin granules possesses antibacterial activity."
Goumon Y., Strub J.-M., Moniatte M., Nulans G., Poteur L., Hubert P., van Dorsselaer A., Aunis D., Metz-Boutigue M.-H.
Eur. J. Biochem. 235:516-525(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 233-261.
Tissue: Chromaffin cell.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
V00109 mRNA. Translation: CAA23443.1.
BC111279 mRNA. Translation: AAI11280.1.
V00108 mRNA. Translation: CAA23442.1.
J00012 mRNA. Translation: AAA30673.1.
PIREQBOA. A93272.
RefSeqNP_776566.1. NM_174141.2.
UniGeneBt.166.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9913.ENSBTAP00000006478.

Proteomic databases

PRIDEP01211.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSBTAT00000006478; ENSBTAP00000006478; ENSBTAG00000004924.
GeneID281387.
KEGGbta:281387.

Organism-specific databases

CTD5179.

Phylogenomic databases

eggNOGNOG40630.
GeneTreeENSGT00530000063761.
HOGENOMHOG000013003.
HOVERGENHBG000063.
InParanoidP01211.
OMAGGFMKKD.
OrthoDBEOG75TMCR.
TreeFamTF332620.

Family and domain databases

InterProIPR006024. Opioid_neupept.
IPR000703. Proenkphlin_A.
[Graphical view]
PANTHERPTHR11438. PTHR11438. 1 hit.
PTHR11438:SF3. PTHR11438:SF3. 1 hit.
PfamPF01160. Opiods_neuropep. 1 hit.
[Graphical view]
PRINTSPR01028. OPIOIDPRCRSR.
PR01029. PENKAPRCRSR.
PROSITEPS01252. OPIOIDS_PRECURSOR. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20805387.

Entry information

Entry namePENK_BOVIN
AccessionPrimary (citable) accession number: P01211
Secondary accession number(s): Q2T9T4
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: February 19, 2014
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families