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P01210 (PENK_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 130. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Proenkephalin-A

Cleaved into the following 8 chains:

  1. Synenkephalin
  2. Met-enkephalin
    Alternative name(s):
    Opioid growth factor
    Short name=OGF
  3. PENK(114-133)
  4. PENK(143-183)
  5. Met-enkephalin-Arg-Gly-Leu
  6. Leu-enkephalin
  7. PENK(237-258)
  8. Met-enkephalin-Arg-Phe
Gene names
Name:PENK
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length267 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Met- and Leu-enkephalins compete with and mimic the effects of opiate drugs. They play a role in a number of physiologic functions, including pain perception and responses to stress. PENK(114-133) and PENK(237-258) increase glutamate release in the striatum. PENK(114-133) decreases GABA concentration in the striatum.

Subcellular location

Secreted.

Post-translational modification

The N-terminal domain contains 6 conserved cysteines thought to be involved in disulfide bonding and/or processing.

Sequence similarities

Belongs to the opioid neuropeptide precursor family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2424 Potential
Peptide25 – 9773Synenkephalin
PRO_0000008242
Peptide100 – 1045Met-enkephalin
PRO_0000008243
Peptide107 – 1115Met-enkephalin
PRO_0000008244
Peptide114 – 13320PENK(114-133) By similarity
PRO_0000377691
Peptide136 – 1405Met-enkephalin
PRO_0000008246
Peptide143 – 18341PENK(143-183) By similarity
PRO_0000377692
Peptide186 – 1938Met-enkephalin-Arg-Gly-Leu
PRO_0000008248
Propeptide196 – 20712
PRO_0000008249
Peptide210 – 2145Met-enkephalin
PRO_0000008250
Propeptide217 – 22711
PRO_0000008251
Peptide230 – 2345Leu-enkephalin
PRO_0000008252
Peptide237 – 25822PENK(237-258) By similarity
PRO_0000377693
Peptide261 – 2677Met-enkephalin-Arg-Phe
PRO_0000008254

Amino acid modifications

Disulfide bond26 ↔ 48 Ref.7
Disulfide bond30 ↔ 52 Ref.7
Disulfide bond33 ↔ 65 Ref.7

Natural variations

Natural variant831T → N.
Corresponds to variant rs11998459 [ dbSNP | Ensembl ].
VAR_048935
Natural variant2471G → D.
Corresponds to variant rs1800567 [ dbSNP | Ensembl ].
VAR_014584

Experimental info

Sequence conflict1191P → S in CAG46627. Ref.4
Sequence conflict1521N → I in CAG46607. Ref.4

Secondary structure

... 267
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P01210 [UniParc].

Last modified July 21, 1986. Version 1.
Checksum: 4189BA600C3FC8EE

FASTA26730,787
        10         20         30         40         50         60 
MARFLTLCTW LLLLGPGLLA TVRAECSQDC ATCSYRLVRP ADINFLACVM ECEGKLPSLK 

        70         80         90        100        110        120 
IWETCKELLQ LSKPELPQDG TSTLRENSKP EESHLLAKRY GGFMKRYGGF MKKMDELYPM 

       130        140        150        160        170        180 
EPEEEANGSE ILAKRYGGFM KKDAEEDDSL ANSSDLLKEL LETGDNRERS HHQDGSDNEE 

       190        200        210        220        230        240 
EVSKRYGGFM RGLKRSPQLE DEAKELQKRY GGFMRRVGRP EWWMDYQKRY GGFLKRFAEA 

       250        260 
LPSDEEGESY SKEVPEMEKR YGGFMRF 

« Hide

References

« Hide 'large scale' references
[1]"Primary structure of the human Met- and Leu-enkephalin precursor and its mRNA."
Comb M., Seeburg P.H., Adelman J., Eiden L., Herbert E.
Nature 295:663-666(1982) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Isolation and structural organization of the human preproenkephalin gene."
Noda M., Teranishi Y., Takahashi H., Toyosato M., Notake M., Nakanishi S., Numa S.
Nature 297:431-434(1982) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Cerebellum.
[4]"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S., Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W., Korn B., Zuo D., Hu Y., LaBaer J.
Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain.
[7]"The structure of synenkephalin (pro-enkephalin 1-73) is dictated by three disulfide bonds."
Lecchi P., Loh Y.P., Snell C.R., Pannell L.K.
Biochem. Biophys. Res. Commun. 232:800-805(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: DISULFIDE BONDS.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
V00509 Genomic DNA. Translation: CAA23767.1.
J00123, J00122 Genomic DNA. Translation: AAB59409.1.
AK314908 mRNA. Translation: BAG37420.1.
CR541808 mRNA. Translation: CAG46607.1.
CR541828 mRNA. Translation: CAG46627.1.
CH471068 Genomic DNA. Translation: EAW86785.1.
BC032505 mRNA. Translation: AAH32505.1.
CCDSCCDS6168.1.
PIREQHUA. A93278.
RefSeqNP_001129162.1. NM_001135690.1.
NP_006202.1. NM_006211.3.
UniGeneHs.339831.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1PLWNMR-A100-104[»]
1PLXNMR-A100-104[»]
2LWCNMR-A261-265[»]
ProteinModelPortalP01210.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid111205. 1 interaction.
IntActP01210. 1 interaction.
STRING9606.ENSP00000324248.

Protein family/group databases

TCDB1.C.89.1.2. the dynorphin channel-forming neuropeptide (dynorphin) family.

PTM databases

PhosphoSiteP01210.

Polymorphism databases

DMDM129770.

Proteomic databases

PaxDbP01210.
PRIDEP01210.

Protocols and materials databases

DNASU5179.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000314922; ENSP00000324248; ENSG00000181195.
ENST00000451791; ENSP00000400894; ENSG00000181195.
GeneID5179.
KEGGhsa:5179.
UCSCuc003xsz.2. human.

Organism-specific databases

CTD5179.
GeneCardsGC08M057349.
HGNCHGNC:8831. PENK.
HPACAB016390.
HPA013138.
MIM131330. gene.
neXtProtNX_P01210.
PharmGKBPA33176.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG40630.
HOGENOMHOG000013003.
HOVERGENHBG000063.
InParanoidP01210.
OMAGGFMKKD.
PhylomeDBP01210.
TreeFamTF332620.

Enzyme and pathway databases

ReactomeREACT_111102. Signal Transduction.

Gene expression databases

ArrayExpressP01210.
BgeeP01210.
CleanExHS_PENK.
GenevestigatorP01210.

Family and domain databases

InterProIPR006024. Opioid_neupept.
IPR000703. Proenkphlin_A.
[Graphical view]
PANTHERPTHR11438. PTHR11438. 1 hit.
PTHR11438:SF3. PTHR11438:SF3. 1 hit.
PfamPF01160. Opiods_neuropep. 1 hit.
[Graphical view]
PRINTSPR01028. OPIOIDPRCRSR.
PR01029. PENKAPRCRSR.
PROSITEPS01252. OPIOIDS_PRECURSOR. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP01210.
GenomeRNAi5179.
NextBio20046.
PMAP-CutDBP01210.
PROP01210.
SOURCESearch...

Entry information

Entry namePENK_HUMAN
AccessionPrimary (citable) accession number: P01210
Secondary accession number(s): B2RC23, Q6FHC6, Q6FHE6
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: July 9, 2014
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 8

Human chromosome 8: entries, gene names and cross-references to MIM