P01179 (NEU1_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Oxytocin-neurophysin 1
Short name=OT-NPI

Cleaved into the following 2 chains:

1. Oxytocin
   Alternative name(s):
   Ocytocin
2. Neurophysin 1

Gene names

Name:	Oxt
Synonyms:	Ot

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	125 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Neurophysin 1 specifically binds oxytocin. Oxytocin causes contraction of the smooth muscle of the uterus and of the mammary gland.
Sequence similarities	Belongs to the vasopressin/oxytocin family.

Ontologies

Keywords
Domain	Signal
Molecular function	Hormone
PTM	Amidation Cleavage on pair of basic residues Disulfide bond
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	drinking behavior Inferred from direct assay. Source: RGD eating behavior Inferred from direct assay. Source: RGD elevation of cytosolic calcium ion concentration Inferred from direct assay. Source: RGD female pregnancy Inferred from expression pattern. Source: RGD grooming behavior Inferred from direct assay. Source: RGD heart development Inferred from expression pattern. Source: RGD hyperosmotic salinity response Inferred from expression pattern. Source: RGD lactation Traceable author statement. Source: RGD male mating behavior Inferred from expression pattern. Source: RGD maternal aggressive behavior Inferred from expression pattern. Source: RGD maternal behavior Inferred from direct assay. Source: RGD memory Inferred from expression pattern. Source: RGD negative regulation of blood pressure Inferred from mutant phenotype. Source: RGD negative regulation of gastric acid secretion Inferred from direct assay. Source: RGD negative regulation of urine volume Inferred from direct assay. Source: RGD parturition Traceable author statement. Source: RGD positive regulation of blood pressure Inferred from direct assay. Source: RGD positive regulation of female receptivity Inferred from direct assay. Source: RGD positive regulation of hindgut contraction Inferred from direct assay. Source: RGD positive regulation of norepinephrine secretion Inferred from direct assay. Source: RGD positive regulation of ossification Inferred from direct assay. Source: RGD positive regulation of penile erection Inferred from direct assay. Source: RGD positive regulation of prostaglandin secretion Inferred from direct assay. Source: RGD positive regulation of renal sodium excretion Inferred from direct assay. Source: RGD positive regulation of synapse assembly Inferred from direct assay. Source: RGD positive regulation of synaptic transmission Inferred from direct assay. Source: RGD positive regulation of uterine smooth muscle contraction Inferred from direct assay. Source: RGD regulation of heart rate Inferred from direct assay. Source: RGD regulation of sensory perception of pain Inferred from direct assay. Source: RGD response to activity Inferred from expression pattern. Source: RGD response to amphetamine Inferred from expression pattern. Source: RGD response to cAMP Inferred from expression pattern. Source: RGD response to cocaine Inferred from expression pattern. Source: RGD response to electrical stimulus Inferred from expression pattern. Source: RGD response to estradiol stimulus Inferred from expression pattern. Source: RGD response to ether Inferred from expression pattern. Source: RGD response to glucocorticoid stimulus Inferred from expression pattern. Source: RGD response to peptide hormone stimulus Inferred from expression pattern. Source: RGD response to progesterone stimulus Inferred from direct assay. Source: RGD response to prostaglandin E stimulus Inferred from expression pattern. Source: RGD response to retinoic acid Inferred from expression pattern. Source: RGD response to sucrose stimulus Inferred from expression pattern. Source: RGD sleep Inferred from direct assay. Source: RGD social behavior Inferred from direct assay. Source: RGD sperm ejaculation Inferred from direct assay. Source: RGD
Cellular component	extracellular space Inferred from direct assay. Source: RGD secretory granule Inferred from direct assay. Source: RGD terminal button Inferred from direct assay. Source: RGD
Molecular function	neurohypophyseal hormone activity Inferred from electronic annotation. Source: InterPro oxytocin receptor binding Inferred from direct assay. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	Ref.8
	Peptide	20 – 28	9	Oxytocin Ref.8		PRO_0000020503
	Chain	32 – 125	94	Neurophysin 1		PRO_0000020504
Amino acid modifications
	Modified residue	28	1	Glycine amide Ref.8
	Disulfide bond	20 ↔ 25		Ref.8
	Disulfide bond	41 ↔ 85		By similarity
	Disulfide bond	44 ↔ 58		By similarity
	Disulfide bond	52 ↔ 75		By similarity
	Disulfide bond	59 ↔ 65		By similarity
	Disulfide bond	92 ↔ 104		By similarity
	Disulfide bond	98 ↔ 116		By similarity
	Disulfide bond	105 ↔ 110		By similarity
Experimental info
	Sequence conflict	100	1	T → A AA sequence Ref.6
	Sequence conflict	124 – 125	2	ER → Q AA sequence Ref.5

Sequences

	Sequence		Length	Mass (Da)
	P01179 [UniParc]. Last modified July 1, 1989. Version 2. Checksum: DC98F90BADC5551F	FASTA	125	12,823
	10 20 30 40 50 60 MACPSLACCL LGLLALTSAC YIQNCPLGGK RAALDLDMRK CLPCGPGGKG RCFGPSICCA 70 80 90 100 110 120 DELGCFVGTA EALRCQEENY LPSPCQSGQK PCGSGGRCAT AGICCSPDGC RTDPACDPES AFSER

References

[1]	"Structure and comparison of the oxytocin and vasopressin genes from rat." Ivell R., Richter D. Proc. Natl. Acad. Sci. U.S.A. 81:2006-2010(1984) [PubMed: 6326097] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The neurohypophyseal hormones vasopressin and oxytocin. Precursor structure, synthesis and regulation." Rehbein M., Hillers M., Mohr E., Ivell R., Morley S., Schmale H., Richter D. Biol. Chem. Hoppe-Seyler 367:695-704(1986) [PubMed: 3768139] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Expression of the vasopressin and oxytocin genes in rats occurs in mutually exclusive sets of hypothalamic neurons." Mohr E., Bahnsen U., Kiessling C., Richter D. FEBS Lett. 242:144-148(1988) [PubMed: 3203740] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"Rat vasopressin and oxytocin genes are linked by a long interspersed repeated DNA element (LINE): sequence and transcriptional analysis of LINE." Schmitz E., Mohr E., Richter D. DNA Cell Biol. 10:81-91(1991) [PubMed: 1706187] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Wistar. Tissue: Liver.
[5]	"Identification of rat neurophysins: complete amino acid sequences of MSEL- and VLDV-neurophysins." Chauvet M.-T., Chauvet J., Acher R. Biochem. Biophys. Res. Commun. 103:595-603(1981) [PubMed: 7036996] [Abstract] Cited for: PROTEIN SEQUENCE OF 32-125.
[6]	"Evolution of neurophysin proteins: partial amino acid sequences of rat neurophysins." North W.G., Mitchell T.I. FEBS Lett. 126:41-44(1981) [PubMed: 7238864] [Abstract] Cited for: PROTEIN SEQUENCE OF 32-100.
[7]	"A comparative study of partial neurophysin protein sequences of cod, guinea pig, rat and sheep." Schlesinger D.H., Pickering B.T., Watkins W.B., Peek J.C., Moore L.G., Audhya T.K., Walter R. FEBS Lett. 80:371-373(1977) [PubMed: 891987] [Abstract] Cited for: PROTEIN SEQUENCE OF 32-64.
[8]	"Evolution of neurohypophyseal hormones: isolation of active principles from rabbits and rats." Chauvet J., Chauvet M.-T., Acher R. Biochimie 53:1099-1104(1971) [PubMed: 5150741] [Abstract] Cited for: PROTEIN SEQUENCE OF 20-28.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	K01701 Genomic DNA. Translation: AAA98733.1. M25649 mRNA. Translation: AAA41773.1. X12792 Genomic DNA. Translation: CAA31281.1. X59496 Genomic DNA. Translation: CAA42085.1.
IPI	IPI00210940.
PIR	NFRT1. A21183.
RefSeq	NP_037128.1. NM_012996.3.
UniGene	Rn.48915.
3D structure databases
ProteinModelPortal	P01179.
SMR	P01179. Positions 32-123.
ModBase	Search...
Protein-protein interaction databases
STRING	P01179.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000028829; ENSRNOP00000028829; ENSRNOG00000021225.
GeneID	25504.
KEGG	rno:25504.
UCSC	M25649. rat.
Organism-specific databases
CTD	5020.
RGD	3238. Oxt.
Phylogenomic databases
eggNOG	roNOG16235.
HOVERGEN	HBG004462.
InParanoid	P01179.
OMA	DGCRTDP.
OrthoDB	EOG451DS6.
PhylomeDB	P01179.
Gene expression databases
ArrayExpress	P01179.
Genevestigator	P01179.
GermOnline	ENSRNOG00000021225. Rattus norvegicus.
Family and domain databases
InterPro	IPR000981. Neurhyp_horm. IPR022423. Neurohypophysial_hormone_CS. [Graphical view]
Gene3D	G3DSA:2.60.9.10. Neurhyp_horm. 1 hit.
KO	K05243.
PANTHER	PTHR11681. PTHR11681. 1 hit.
Pfam	PF00220. Hormone_4. 1 hit. PF00184. Hormone_5. 1 hit. [Graphical view]
PIRSF	PIRSF001815. Nonapeptide_hormone_precursor. 1 hit.
PRINTS	PR00831. NEUROPHYSIN.
ProDom	PD001676. Neurhyp_horm. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00003. NH. 1 hit. [Graphical view]
SUPFAM	SSF49606. Neurhyp_horm. 1 hit.
PROSITE	PS00264. NEUROHYPOPHYS_HORM. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	606917.
PMAP-CutDB	P01179.

Entry information

Entry name

NEU1_RAT

Accession

Primary (citable) accession number: P01179
Secondary accession number(s): Q53WU2

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 1, 1989
Last modified:	November 16, 2011
This is version 103 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program