Reviewed, UniProtKB/Swiss-Prot P01178 (NEU1_HUMAN)

Names and origin

Protein names

Recommended name:
    Oxytocin-neurophysin 1
      Short name=OT-NPI
Cleaved into the following 2 chains:
    1- Recommended name:
            Oxytocin
        Alternative name(s):
            Ocytocin
    2- Recommended name:
            Neurophysin 1

Gene names

Name:	OXT
Synonyms:	OT

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	125 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Neurophysin 1 specifically binds oxytocin. Oxytocin causes contraction of the smooth muscle of the uterus and of the mammary gland.
Subcellular location	Secreted.
Pharmaceutical use	Oxytocin is available under the names Pitocin (Parke-Davis) and Syntocinon (Sandoz). Used to artificially speed or induce labor.
Sequence similarities	Belongs to the vasopressin/oxytocin family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Hormone
PTM	Amidation Cleavage on pair of basic residues Disulfide bond
Technical term	Direct protein sequencing Pharmaceutical
Gene Ontology (GO)
Biological process	signal transduction Traceable author statement. Source: ProtInc
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	neurohypophyseal hormone activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
NCK1	P16333	1	EBI-1762651,EBI-389883

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	Ref.10
	Peptide	20 – 28	9	Oxytocin		PRO_0000020495
	Chain	32 – 125	94	Neurophysin 1		PRO_0000020496
Amino acid modifications
	Modified residue	28	1	Glycine amide
	Disulfide bond	20 ↔ 25
	Disulfide bond	41 ↔ 85		By similarity
	Disulfide bond	44 ↔ 58		By similarity
	Disulfide bond	52 ↔ 75		By similarity
	Disulfide bond	59 ↔ 65		By similarity
	Disulfide bond	92 ↔ 104		By similarity
	Disulfide bond	98 ↔ 116		By similarity
	Disulfide bond	105 ↔ 110		By similarity
Experimental info
	Sequence conflict	94	1	S → G in AAA59979. Ref.6
	Sequence conflict	100 – 101	2	VL → AA AA sequence Ref.9
	Sequence conflict	100	1	Missing in AAA98806. Ref.2
	Sequence conflict	124	1	Q → L AA sequence Ref.9

Sequences

	Sequence		Length	Mass (Da)
	P01178-1 [UniParc]. Last modified January 1, 1988. Version 1. Checksum: C65BB544731A0E7C	FASTA	125	12,722
	10 20 30 40 50 60 MAGPSLACCL LGLLALTSAC YIQNCPLGGK RAAPDLDVRK CLPCGPGGKG RCFGPNICCA 70 80 90 100 110 120 EELGCFVGTA EALRCQEENY LPSPCQSGQK ACGSGGRCAV LGLCCSPDGC HADPACDAEA TFSQR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The neurohypophyseal hormones vasopressin and oxytocin. Precursor structure, synthesis and regulation." Rehbein M., Hillers M., Mohr E., Ivell R., Morley S., Schmale H., Richter D. Biol. Chem. Hoppe-Seyler 367:695-704(1986) [PubMed: 3768139] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The human vasopressin gene is linked to the oxytocin gene and is selectively expressed in a cultured lung cancer cell line." Sausville E., Carney D., Battey J. J. Biol. Chem. 260:10236-10241(1985) [PubMed: 2991279] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Lack of sequence polymorphism in the oxytocin gene of autistic patients." Grunwald W.C. Jr., Cool D.R. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"The DNA sequence and comparative analysis of human chromosome 20." Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P., Bird C.P., Blakey S.E. , Bridgeman A.M., Brown A.J., Buck D., Burrill W.D., Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G., Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E., Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D., Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P., Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E., Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J., Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D., Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S., Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D., Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A., Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T., Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I., Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M., Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D., Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M., Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A., Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L., Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L., Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J. Nature 414:865-871(2001) [PubMed: 11780052] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung.
[6]	"Expression of the oxytocin and vasopressin genes in human and baboon gonadal tissues." Ivell R., Furuya K., Brackmann B., Dawood Y., Khan-Dawood F. Endocrinology 127:2990-2996(1990) [PubMed: 2249637] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 25-110.
[7]	"Expression of the vasopressin and oxytocin genes in human hypothalami." Mohr E., Hillers M., Ivell R., Haulica I.D., Richter D. FEBS Lett. 193:12-16(1985) [PubMed: 4065330] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 80-125.
[8]	"Identification of human neurophysins: complete amino acid sequences of MSEL- and VLDV-neurophysins." Chauvet M.-T., Hurpet D., Chauvet J., Acher R. Proc. Natl. Acad. Sci. U.S.A. 80:2839-2843(1983) [PubMed: 6574452] [Abstract] Cited for: PROTEIN SEQUENCE OF 32-125.
[9]	"A comparative study of mammalian neurophysin protein sequences." Schlesinger D.H., Audhya T.K. FEBS Lett. 128:325-328(1981) [PubMed: 7262323] [Abstract] Cited for: PROTEIN SEQUENCE OF 32-125.
[10]	"On the nature of oxytocin and vasopressin from human pituitary." Light A., du Vigneaud V. Proc. Soc. Exp. Biol. Med. 98:692-696(1958) [PubMed: 13591312] [Abstract] Cited for: PROTEIN SEQUENCE OF 20-28.
+	Additional computationally mapped references.

Web resources

Wikipedia

Oxytocin entry

Cross-references

Sequence databases
EMBLGenBankDDBJ	M25650 mRNA. Translation: AAA59977.1. M11186 Genomic DNA. Translation: AAA98806.1. AY082910 Genomic DNA. Translation: AAL92860.1. AL160414 Genomic DNA. Translation: CAC10204.1. BC069144 mRNA. Translation: AAH69144.1. BC101841 mRNA. Translation: AAI01842.1. BC101843 mRNA. Translation: AAI01844.1. X03173 mRNA. Translation: CAA26936.1. M62611 mRNA. Translation: AAA59979.1.
IPI	IPI00000144.
PIR	NFHU1. A94676.
RefSeq	NP_000906.1.
UniGene	Hs.113216
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	P01178. 1 interaction.
Proteomic databases
PeptideAtlas	P01178.
PRIDE	P01178.
Genome annotation databases
Ensembl	ENSG00000101405. Homo sapiens. [Contig view]
GeneID	5020.
KEGG	hsa:5020.
Organism-specific databases
GeneCards	GC20P003000.
H-InvDB	HIX0040598.
HGNC	HGNC:8528. OXT.
MIM	167050. gene.
PharmGKB	PA32857.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	P01178.
HOVERGEN	P01178.
OMA	P01178. DGCRTDP.
Gene expression databases
ArrayExpress	P01178.
Bgee	P01178.
CleanEx	HS_OXT.
GermOnline	ENSG00000101405. Homo sapiens.
Family and domain databases
InterPro	IPR000981. Neurhyp_horm. IPR016321. Nonapeptide_hormone_prcur. [Graphical view]
Gene3D	G3DSA:2.60.9.10. Neurhyp_horm. 1 hit.
Pfam	PF00220. Hormone_4. 1 hit. PF00184. Hormone_5. 1 hit. [Graphical view]
PIRSF	PIRSF001815. Nonapeptide_hormone_precursor. 1 hit.
PRINTS	PR00831. NEUROPHYSIN.
ProDom	PD001676. Neurhyp_horm. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00003. NH. 1 hit. [Graphical view]
PROSITE	PS00264. NEUROHYPOPHYS_HORM. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
DrugBank	DB00107. Oxytocin.
NextBio	19328.
PMAP-CutDB	P01178.
SOURCE	Search...

Entry information

Entry name

NEU1_HUMAN

Accession

Primary (citable) accession number: P01178
Secondary accession number(s): Q3MIG0

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	January 1, 1988
Last modified:	June 16, 2009
This is version 95 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)