ID   NEU1_BOVIN              Reviewed;         125 AA.
AC   P01175; A5PJ78; P01188;
DT   21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1988, sequence version 1.
DT   16-NOV-2011, entry version 99.
DE   RecName: Full=Oxytocin-neurophysin 1;
DE            Short=OT-NPI;
DE   Contains:
DE     RecName: Full=Oxytocin;
DE     AltName: Full=Ocytocin;
DE   Contains:
DE     RecName: Full=Neurophysin 1;
DE   Flags: Precursor;
GN   Name=OXT;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Laurasiatheria; Cetartiodactyla; Ruminantia;
OC   Pecora; Bovidae; Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=6687626; DOI=10.1038/302342a0;
RA   Land H., Grez M., Ruppert S., Schmale H., Rehbein M., Richter D.,
RA   Schuetz G.;
RT   "Deduced amino acid sequence from the bovine oxytocin-neurophysin I
RT   precursor cDNA.";
RL   Nature 302:342-344(1983).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   MEDLINE=84168167; PubMed=6709064; DOI=10.1038/308554a0;
RA   Ruppert S., Scherer G., Schuetz G.;
RT   "Recent gene conversion involving bovine vasopressin and oxytocin
RT   precursor genes suggested by nucleotide sequence.";
RL   Nature 308:554-557(1984).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   MEDLINE=87026235; PubMed=3768139;
RA   Rehbein M., Hillers M., Mohr E., Ivell R., Morley S., Schmale H.,
RA   Richter D.;
RT   "The neurohypophyseal hormones vasopressin and oxytocin. Precursor
RT   structure, synthesis and regulation.";
RL   Biol. Chem. Hoppe-Seyler 367:695-704(1986).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Hereford; TISSUE=Hypothalamus;
RG   NIH - Mammalian Gene Collection (MGC) project;
RL   Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases.
RN   [5]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 15-125.
RX   MEDLINE=85051301; PubMed=6209133;
RA   Ivell R., Richter D.;
RT   "The gene for the hypothalamic peptide hormone oxytocin is highly
RT   expressed in the bovine corpus luteum: biosynthesis, structure and
RT   sequence analysis.";
RL   EMBO J. 3:2351-2354(1984).
RN   [6]
RP   PROTEIN SEQUENCE OF 20-28, DISULFIDE BONDS, AND AMIDATION AT GLY-28.
RX   PubMed=13129273;
RA   du Vigneaud V., Ressler C., Trippett S.;
RT   "The sequence of amino acids in oxytocin, with a proposal for the
RT   structure of oxytocin.";
RL   J. Biol. Chem. 205:949-957(1953).
RN   [7]
RP   PROTEIN SEQUENCE OF 20-28.
RA   Tuppy H., Michl H.;
RT   "About the chemical structure of oxytocin.";
RL   Monatsh. Chem. 84:1011-1020(1953).
RN   [8]
RP   PROTEIN SEQUENCE OF 32-124.
RX   MEDLINE=78218154; PubMed=670174;
RA   Schlesinger D.H., Audhya T.K., Walter R.;
RT   "Complete amino acid sequence of bovine neurophysin-I. A major
RT   secretory product of the posterior pituitary.";
RL   J. Biol. Chem. 253:5019-5024(1978).
RN   [9]
RP   PROTEIN SEQUENCE OF 32-124.
RX   MEDLINE=79148658; PubMed=428540; DOI=10.1016/0014-5793(79)80146-5;
RA   Chauvet M.-T., Codogno P., Chauvet J., Acher R.;
RT   "Comparison between MSEL- and VLDV-neurophysins. Complete amino acid
RT   sequences of porcine and bovine VLDV-neurophysins.";
RL   FEBS Lett. 98:37-40(1979).
RN   [10]
RP   DISULFIDE BONDS.
RX   PubMed=2911588; DOI=10.1073/pnas.86.2.429;
RA   Burman S., Wellner D., Chait B., Chaudhary T., Breslow E.;
RT   "Complete assignment of neurophysin disulfides indicates pairing in
RT   two separate domains.";
RL   Proc. Natl. Acad. Sci. U.S.A. 86:429-433(1989).
RN   [11]
RP   X-RAY CRYSTALLOGRAPHY (1.04 ANGSTROMS) OF OXYTOCIN, DISULFIDE BOND,
RP   AND AMIDATION AT GLY-28.
RX   MEDLINE=86179884; PubMed=3008332; DOI=10.1126/science.3008332;
RA   Wood S.P., Tickle I.J., Treharne A.M., Pitts J.E., Mascarenhas Y.,
RA   Li J.Y., Husain J., Cooper S., Blundell T.L., Hruby V.J.;
RT   "Crystal structure analysis of deamino-oxytocin: conformational
RT   flexibility and receptor binding.";
RL   Science 232:633-636(1986).
CC   -!- FUNCTION: Neurophysin 1 specifically binds oxytocin.
CC   -!- FUNCTION: Oxytocin causes contraction of the smooth muscle of the
CC       uterus and of the mammary gland.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- SIMILARITY: Belongs to the vasopressin/oxytocin family.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAA23450.1; Type=Erroneous initiation;
CC       Sequence=CAA25194.1; Type=Erroneous gene model prediction;
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DR   EMBL; X00502; CAA25194.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; M25648; AAA30680.1; -; mRNA.
DR   EMBL; BC141997; AAI41998.1; -; mRNA.
DR   EMBL; V00114; CAA23448.1; -; mRNA.
DR   EMBL; V00114; CAA23450.1; ALT_INIT; mRNA.
DR   EMBL; V00114; CAA23449.1; ALT_SEQ; mRNA.
DR   EMBL; X00950; CAA25462.1; -; mRNA.
DR   IPI; IPI00717628; -.
DR   PIR; S07332; NFBO1.
DR   RefSeq; NP_789825.1; NM_176855.1.
DR   UniGene; Bt.183; -.
DR   PDB; 1L5C; NMR; -; A=32-123.
DR   PDB; 1L5D; NMR; -; A=32-123.
DR   PDB; 1XY1; X-ray; 1.04 A; A/B=21-28.
DR   PDB; 1XY2; X-ray; 1.20 A; A=21-28.
DR   PDB; 2HNU; X-ray; 2.00 A; A/B/C/D/E=38-118.
DR   PDB; 2HNV; X-ray; 2.50 A; A/B/C/D/E=38-118.
DR   PDB; 2HNW; X-ray; 2.90 A; A/B/C/D/E=38-117.
DR   PDBsum; 1L5C; -.
DR   PDBsum; 1L5D; -.
DR   PDBsum; 1XY1; -.
DR   PDBsum; 1XY2; -.
DR   PDBsum; 2HNU; -.
DR   PDBsum; 2HNV; -.
DR   PDBsum; 2HNW; -.
DR   ProteinModelPortal; P01175; -.
DR   SMR; P01175; 38-118.
DR   STRING; P01175; -.
DR   Ensembl; ENSBTAT00000010554; ENSBTAP00000010554; ENSBTAG00000008026.
DR   GeneID; 280888; -.
DR   KEGG; bta:280888; -.
DR   CTD; 5020; -.
DR   eggNOG; maNOG20315; -.
DR   GeneTree; ENSGT00390000004511; -.
DR   HOVERGEN; HBG004462; -.
DR   InParanoid; P01175; -.
DR   OMA; DGCRTDP; -.
DR   OrthoDB; EOG451DS6; -.
DR   PhylomeDB; P01175; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0005185; F:neurohypophyseal hormone activity; IEA:InterPro.
DR   InterPro; IPR000981; Neurhyp_horm.
DR   InterPro; IPR022423; Neurohypophysial_hormone_CS.
DR   Gene3D; G3DSA:2.60.9.10; Neurhyp_horm; 1.
DR   KO; K05243; -.
DR   PANTHER; PTHR11681; PTHR11681; 1.
DR   Pfam; PF00220; Hormone_4; 1.
DR   Pfam; PF00184; Hormone_5; 1.
DR   PIRSF; PIRSF001815; Nonapeptide_hormone_precursor; 1.
DR   PRINTS; PR00831; NEUROPHYSIN.
DR   ProDom; PD001676; Neurhyp_horm; 1.
DR   SMART; SM00003; NH; 1.
DR   SUPFAM; SSF49606; Neurhyp_horm; 1.
DR   PROSITE; PS00264; NEUROHYPOPHYS_HORM; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Amidation; Cleavage on pair of basic residues;
KW   Complete proteome; Direct protein sequencing; Disulfide bond; Hormone;
KW   Reference proteome; Secreted; Signal.
FT   SIGNAL        1     19
FT   PEPTIDE      20     28       Oxytocin.
FT                                /FTId=PRO_0000020491.
FT   CHAIN        32    125       Neurophysin 1.
FT                                /FTId=PRO_0000020492.
FT   MOD_RES      28     28       Glycine amide.
FT   DISULFID     20     25
FT   DISULFID     41     85
FT   DISULFID     44     58
FT   DISULFID     52     75
FT   DISULFID     59     65
FT   DISULFID     92    104
FT   DISULFID     98    116
FT   DISULFID    105    110
FT   CONFLICT    124    124       Q -> L (in Ref. 9; AA sequence).
FT   STRAND       36     38
FT   STRAND       43     45
FT   HELIX        46     48
FT   STRAND       50     54
FT   STRAND       57     60
FT   TURN         61     63
FT   STRAND       64     69
FT   HELIX        70     80
FT   STRAND       89     92
FT   TURN         93     95
FT   STRAND       96    100
FT   STRAND      103    106
FT   STRAND      109    112
FT   HELIX       114    116
SQ   SEQUENCE   125 AA;  12665 MW;  3B4E4E79A0B7F032 CRC64;
     MAGSSLACCL LGLLALTSAC YIQNCPLGGK RAVLDLDVRT CLPCGPGGKG RCFGPSICCG
     DELGCFVGTA EALRCQEENY LPSPCQSGQK PCGSGGRCAA AGICCSPDGC HEDPACDPEA
     AFSQH
//