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Protein

Oxytocin-neurophysin 1

Gene

OXT

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Neurophysin 1 specifically binds oxytocin.
Oxytocin causes contraction of the smooth muscle of the uterus and of the mammary gland.

GO - Biological processi

  • response to estrogen Source: CACAO
  • response to external biotic stimulus Source: AgBase
  • response to food Source: AgBase
  • response to mechanical stimulus Source: AgBase
Complete GO annotation...

Keywords - Molecular functioni

Hormone

Enzyme and pathway databases

ReactomeiR-BTA-388479. Vasopressin-like receptors.
R-BTA-416476. G alpha (q) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
Oxytocin-neurophysin 1
Short name:
OT-NPI
Cleaved into the following 2 chains:
Alternative name(s):
Ocytocin
Gene namesi
Name:OXT
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 13

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: Ensembl
  • extracellular region Source: UniProtKB-SubCell
  • extracellular space Source: AgBase
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 19192 PublicationsAdd
BLAST
Peptidei20 – 289OxytocinPRO_0000020491
Chaini32 – 12594Neurophysin 1PRO_0000020492Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi20 ↔ 252 Publications
Modified residuei28 – 281Glycine amide2 Publications
Disulfide bondi41 ↔ 85Combined sources
Disulfide bondi44 ↔ 58Combined sources
Disulfide bondi52 ↔ 75Combined sources
Disulfide bondi59 ↔ 65Combined sources
Disulfide bondi92 ↔ 104Combined sources
Disulfide bondi98 ↔ 116Combined sources
Disulfide bondi105 ↔ 110Combined sources

Keywords - PTMi

Amidation, Cleavage on pair of basic residues, Disulfide bond

Proteomic databases

PaxDbiP01175.

Interactioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000010554.

Structurei

Secondary structure

1
125
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi36 – 383Combined sources
Beta strandi43 – 453Combined sources
Helixi46 – 483Combined sources
Beta strandi50 – 545Combined sources
Beta strandi57 – 604Combined sources
Turni61 – 633Combined sources
Beta strandi64 – 696Combined sources
Helixi70 – 8011Combined sources
Beta strandi89 – 924Combined sources
Turni93 – 953Combined sources
Beta strandi96 – 1005Combined sources
Beta strandi103 – 1064Combined sources
Beta strandi109 – 1124Combined sources
Helixi114 – 1163Combined sources
Beta strandi117 – 1215Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1L5CNMR-A32-123[»]
1L5DNMR-A32-123[»]
1XY1X-ray1.04A/B21-28[»]
1XY2X-ray1.20A21-28[»]
2HNUX-ray2.00A/B/C/D/E38-118[»]
2HNVX-ray2.50A/B/C/D/E38-118[»]
2HNWX-ray2.90A/B/C/D/E38-117[»]
2LBHNMR-A/B32-123[»]
2LBNNMR-A32-123[»]
ProteinModelPortaliP01175.
SMRiP01175. Positions 38-118.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP01175.

Family & Domainsi

Sequence similaritiesi

Belongs to the vasopressin/oxytocin family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410J3A8. Eukaryota.
ENOG41124QF. LUCA.
GeneTreeiENSGT00390000004511.
HOGENOMiHOG000113768.
HOVERGENiHBG004462.
InParanoidiP01175.
KOiK05243.
OMAiDGCRTDP.
OrthoDBiEOG7NW6C5.
TreeFamiTF333018.

Family and domain databases

Gene3Di2.60.9.10. 1 hit.
InterProiIPR000981. Neurhyp_horm.
IPR022423. Neurohypophysial_hormone_CS.
[Graphical view]
PANTHERiPTHR11681. PTHR11681. 1 hit.
PfamiPF00220. Hormone_4. 1 hit.
PF00184. Hormone_5. 1 hit.
[Graphical view]
PIRSFiPIRSF001815. Nonapeptide_hormone_precursor. 1 hit.
PRINTSiPR00831. NEUROPHYSIN.
ProDomiPD001676. Neurhyp_horm. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00003. NH. 1 hit.
[Graphical view]
SUPFAMiSSF49606. SSF49606. 1 hit.
PROSITEiPS00264. NEUROHYPOPHYS_HORM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P01175-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGSSLACCL LGLLALTSAC YIQNCPLGGK RAVLDLDVRT CLPCGPGGKG
60 70 80 90 100
RCFGPSICCG DELGCFVGTA EALRCQEENY LPSPCQSGQK PCGSGGRCAA
110 120
AGICCSPDGC HEDPACDPEA AFSQH
Length:125
Mass (Da):12,665
Last modified:January 1, 1988 - v1
Checksum:i3B4E4E79A0B7F032
GO

Sequence cautioni

The sequence CAA23450.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence CAA25194.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti124 – 1241Q → L AA sequence (PubMed:428540).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X00502 Genomic DNA. Translation: CAA25194.1. Sequence problems.
M25648 mRNA. Translation: AAA30680.1.
BC141997 mRNA. Translation: AAI41998.1.
V00114 mRNA. Translation: CAA23448.1.
V00114 mRNA. Translation: CAA23450.1. Different initiation.
V00114 mRNA. Translation: CAA23449.1. Sequence problems.
X00950 mRNA. Translation: CAA25462.1.
PIRiS07332. NFBO1.
RefSeqiNP_789825.1. NM_176855.1.
UniGeneiBt.183.

Genome annotation databases

EnsembliENSBTAT00000010554; ENSBTAP00000010554; ENSBTAG00000008026.
GeneIDi280888.
KEGGibta:280888.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X00502 Genomic DNA. Translation: CAA25194.1. Sequence problems.
M25648 mRNA. Translation: AAA30680.1.
BC141997 mRNA. Translation: AAI41998.1.
V00114 mRNA. Translation: CAA23448.1.
V00114 mRNA. Translation: CAA23450.1. Different initiation.
V00114 mRNA. Translation: CAA23449.1. Sequence problems.
X00950 mRNA. Translation: CAA25462.1.
PIRiS07332. NFBO1.
RefSeqiNP_789825.1. NM_176855.1.
UniGeneiBt.183.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1L5CNMR-A32-123[»]
1L5DNMR-A32-123[»]
1XY1X-ray1.04A/B21-28[»]
1XY2X-ray1.20A21-28[»]
2HNUX-ray2.00A/B/C/D/E38-118[»]
2HNVX-ray2.50A/B/C/D/E38-118[»]
2HNWX-ray2.90A/B/C/D/E38-117[»]
2LBHNMR-A/B32-123[»]
2LBNNMR-A32-123[»]
ProteinModelPortaliP01175.
SMRiP01175. Positions 38-118.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000010554.

Proteomic databases

PaxDbiP01175.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000010554; ENSBTAP00000010554; ENSBTAG00000008026.
GeneIDi280888.
KEGGibta:280888.

Organism-specific databases

CTDi5020.

Phylogenomic databases

eggNOGiENOG410J3A8. Eukaryota.
ENOG41124QF. LUCA.
GeneTreeiENSGT00390000004511.
HOGENOMiHOG000113768.
HOVERGENiHBG004462.
InParanoidiP01175.
KOiK05243.
OMAiDGCRTDP.
OrthoDBiEOG7NW6C5.
TreeFamiTF333018.

Enzyme and pathway databases

ReactomeiR-BTA-388479. Vasopressin-like receptors.
R-BTA-416476. G alpha (q) signalling events.

Miscellaneous databases

EvolutionaryTraceiP01175.
NextBioi20805023.

Family and domain databases

Gene3Di2.60.9.10. 1 hit.
InterProiIPR000981. Neurhyp_horm.
IPR022423. Neurohypophysial_hormone_CS.
[Graphical view]
PANTHERiPTHR11681. PTHR11681. 1 hit.
PfamiPF00220. Hormone_4. 1 hit.
PF00184. Hormone_5. 1 hit.
[Graphical view]
PIRSFiPIRSF001815. Nonapeptide_hormone_precursor. 1 hit.
PRINTSiPR00831. NEUROPHYSIN.
ProDomiPD001676. Neurhyp_horm. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00003. NH. 1 hit.
[Graphical view]
SUPFAMiSSF49606. SSF49606. 1 hit.
PROSITEiPS00264. NEUROHYPOPHYS_HORM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Deduced amino acid sequence from the bovine oxytocin-neurophysin I precursor cDNA."
    Land H., Grez M., Ruppert S., Schmale H., Rehbein M., Richter D., Schuetz G.
    Nature 302:342-344(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Recent gene conversion involving bovine vasopressin and oxytocin precursor genes suggested by nucleotide sequence."
    Ruppert S., Scherer G., Schuetz G.
    Nature 308:554-557(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "The neurohypophyseal hormones vasopressin and oxytocin. Precursor structure, synthesis and regulation."
    Rehbein M., Hillers M., Mohr E., Ivell R., Morley S., Schmale H., Richter D.
    Biol. Chem. Hoppe-Seyler 367:695-704(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  4. NIH - Mammalian Gene Collection (MGC) project
    Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Hypothalamus.
  5. "The gene for the hypothalamic peptide hormone oxytocin is highly expressed in the bovine corpus luteum: biosynthesis, structure and sequence analysis."
    Ivell R., Richter D.
    EMBO J. 3:2351-2354(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 15-125.
  6. "The sequence of amino acids in oxytocin, with a proposal for the structure of oxytocin."
    du Vigneaud V., Ressler C., Trippett S.
    J. Biol. Chem. 205:949-957(1953) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 20-28, DISULFIDE BONDS, AMIDATION AT GLY-28.
  7. "About the chemical structure of oxytocin."
    Tuppy H., Michl H.
    Monatsh. Chem. 84:1011-1020(1953)
    Cited for: PROTEIN SEQUENCE OF 20-28.
  8. "Complete amino acid sequence of bovine neurophysin-I. A major secretory product of the posterior pituitary."
    Schlesinger D.H., Audhya T.K., Walter R.
    J. Biol. Chem. 253:5019-5024(1978) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 32-124.
  9. "Comparison between MSEL- and VLDV-neurophysins. Complete amino acid sequences of porcine and bovine VLDV-neurophysins."
    Chauvet M.-T., Codogno P., Chauvet J., Acher R.
    FEBS Lett. 98:37-40(1979) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 32-124.
  10. "Complete assignment of neurophysin disulfides indicates pairing in two separate domains."
    Burman S., Wellner D., Chait B., Chaudhary T., Breslow E.
    Proc. Natl. Acad. Sci. U.S.A. 86:429-433(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISULFIDE BONDS.
  11. "Crystal structure analysis of deamino-oxytocin: conformational flexibility and receptor binding."
    Wood S.P., Tickle I.J., Treharne A.M., Pitts J.E., Mascarenhas Y., Li J.Y., Husain J., Cooper S., Blundell T.L., Hruby V.J.
    Science 232:633-636(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.04 ANGSTROMS) OF OXYTOCIN, DISULFIDE BOND, AMIDATION AT GLY-28.

Entry informationi

Entry nameiNEU1_BOVIN
AccessioniPrimary (citable) accession number: P01175
Secondary accession number(s): A5PJ78, P01188
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 1, 1988
Last modified: January 20, 2016
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.