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Protein

Natriuretic peptides A

Gene

Nppa

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Hormone playing a key role in cardiovascular homeostasis through regulation of natriuresis, diuresis, and vasodilation. Also plays a role in female pregnancy by promoting trophoblast invasion and spiral artery remodeling in uterus. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3 (By similarity).By similarity

GO - Molecular functioni

  • hormone activity Source: RGD
  • peptide hormone receptor binding Source: RGD
  • receptor binding Source: RGD

GO - Biological processi

  • cardiac muscle hypertrophy in response to stress Source: Ensembl
  • cell growth involved in cardiac muscle cell development Source: RGD
  • cellular response to mechanical stimulus Source: RGD
  • cGMP biosynthetic process Source: RGD
  • female pregnancy Source: UniProtKB
  • heart development Source: UniProtKB
  • multicellular organismal water homeostasis Source: RGD
  • negative regulation of cell growth Source: RGD
  • negative regulation of collecting lymphatic vessel constriction Source: RGD
  • negative regulation of systemic arterial blood pressure Source: RGD
  • neuropeptide signaling pathway Source: Ensembl
  • positive regulation of cardiac muscle contraction Source: Ensembl
  • positive regulation of delayed rectifier potassium channel activity Source: BHF-UCL
  • positive regulation of heart rate Source: Ensembl
  • positive regulation of potassium ion export across plasma membrane Source: BHF-UCL
  • receptor guanylyl cyclase signaling pathway Source: UniProtKB
  • regulation of atrial cardiac muscle cell membrane repolarization Source: Ensembl
  • regulation of blood pressure Source: UniProtKB
  • regulation of blood vessel size Source: UniProtKB-KW
  • regulation of body fluid levels Source: RGD
  • regulation of calcium ion transmembrane transport via high voltage-gated calcium channel Source: BHF-UCL
  • regulation of high voltage-gated calcium channel activity Source: BHF-UCL
  • response to hypoxia Source: RGD
  • response to insulin Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Hormone, Vasoactive

Enzyme and pathway databases

ReactomeiR-RNO-5578768. Physiological factors.

Names & Taxonomyi

Protein namesi
Recommended name:
Natriuretic peptides A
Alternative name(s):
Prepronatriodilatin
Cleaved into the following 6 chains:
Alternative name(s):
Atrial natriuretic peptide
Short name:
ANP
Alternative name(s):
Atriopeptin I
Alternative name(s):
Atriopeptin II
Alternative name(s):
Atriopeptin III
Gene namesi
Name:Nppa
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 5

Organism-specific databases

RGDi3193. Nppa.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: RGD
  • extracellular space Source: Ensembl
  • mast cell granule Source: RGD
  • nucleus Source: RGD
  • perinuclear region of cytoplasm Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL4287.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 241 PublicationAdd BLAST24
PropeptideiPRO_000000150525 – 122CuratedAdd BLAST98
PeptideiPRO_0000391785123 – 150Atrial natriuretic factorBy similarityAdd BLAST28
PeptideiPRO_0000001506126 – 150Auriculin-B1 PublicationAdd BLAST25
PeptideiPRO_0000001507126 – 149Auriculin-A1 PublicationAdd BLAST24
PeptideiPRO_0000001508127 – 150Atriopeptin-31 PublicationAdd BLAST24
PeptideiPRO_0000001509127 – 149Atriopeptin-21 PublicationAdd BLAST23
PeptideiPRO_0000001510127 – 147Atriopeptin-11 PublicationAdd BLAST21

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi129 ↔ 1451 Publication

Post-translational modificationi

Cleaved by CORIN upon secretion to produce the functional hormone (By similarity).By similarity
Atrial natriuretic factor: Cleaved by MME. The cleavage initiates degradation of the factor and thereby regulate its activity.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei122 – 123Cleavage; by CORINBy similarity2
Sitei129 – 130Cleavage; by MME1 Publication2

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond

Proteomic databases

PaxDbiP01161.
PRIDEiP01161.

PTM databases

iPTMnetiP01161.
PhosphoSitePlusiP01161.

Miscellaneous databases

PMAP-CutDBP01161.

Expressioni

Gene expression databases

BgeeiENSRNOG00000008176.
ExpressionAtlasiP01161. baseline and differential.
GenevisibleiP01161. RN.

Interactioni

Subunit structurei

Interacts with NPR1.1 Publication

GO - Molecular functioni

  • hormone activity Source: RGD
  • peptide hormone receptor binding Source: RGD
  • receptor binding Source: RGD

Protein-protein interaction databases

IntActiP01161. 1 interactor.
STRINGi10116.ENSRNOP00000011005.

Chemistry databases

BindingDBiP01161.

Structurei

Secondary structure

1152
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni136 – 139Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1T34X-ray2.95H129-149[»]
SMRiP01161.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP01161.

Family & Domainsi

Sequence similaritiesi

Belongs to the natriuretic peptide family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IW5F. Eukaryota.
ENOG410ZUY6. LUCA.
GeneTreeiENSGT00510000048659.
HOGENOMiHOG000033937.
HOVERGENiHBG004227.
InParanoidiP01161.
KOiK12334.
OMAiGCNSFRY.
OrthoDBiEOG091G0SV3.
PhylomeDBiP01161.
TreeFamiTF106304.

Family and domain databases

InterProiIPR000663. Natr_peptide.
IPR030480. Natr_peptide_CS.
IPR002407. Natriuretic_peptide_atrial.
[Graphical view]
PfamiPF00212. ANP. 1 hit.
[Graphical view]
PRINTSiPR00711. ANATPEPTIDE.
PR00710. NATPEPTIDES.
SMARTiSM00183. NAT_PEP. 1 hit.
[Graphical view]
PROSITEiPS00263. NATRIURETIC_PEPTIDE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P01161-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGSFSITKGF FLFLAFWLPG HIGANPVYSA VSNTDLMDFK NLLDHLEEKM
60 70 80 90 100
PVEDEVMPPQ ALSEQTDEAG AALSSLSEVP PWTGEVNPSQ RDGGALGRGP
110 120 130 140 150
WDPSDRSALL KSKLRALLAG PRSLRRSSCF GGRIDRIGAQ SGLGCNSFRY

RR
Length:152
Mass (Da):16,556
Last modified:July 21, 1986 - v1
Checksum:iD8AAB2191E29519F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M15868 mRNA. Translation: AAA40736.1.
X00665 mRNA. Translation: CAA25285.1.
K02062 Genomic DNA. Translation: AAA40735.1.
X01118 mRNA. Translation: CAA25586.1.
M27498 mRNA. Translation: AAA40737.1.
PIRiA22570. AWRT.
RefSeqiNP_036744.1. NM_012612.2.
UniGeneiRn.2004.

Genome annotation databases

EnsembliENSRNOT00000011005; ENSRNOP00000011005; ENSRNOG00000008176.
GeneIDi24602.
KEGGirno:24602.
UCSCiRGD:3193. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M15868 mRNA. Translation: AAA40736.1.
X00665 mRNA. Translation: CAA25285.1.
K02062 Genomic DNA. Translation: AAA40735.1.
X01118 mRNA. Translation: CAA25586.1.
M27498 mRNA. Translation: AAA40737.1.
PIRiA22570. AWRT.
RefSeqiNP_036744.1. NM_012612.2.
UniGeneiRn.2004.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1T34X-ray2.95H129-149[»]
SMRiP01161.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP01161. 1 interactor.
STRINGi10116.ENSRNOP00000011005.

Chemistry databases

BindingDBiP01161.
ChEMBLiCHEMBL4287.

PTM databases

iPTMnetiP01161.
PhosphoSitePlusiP01161.

Proteomic databases

PaxDbiP01161.
PRIDEiP01161.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000011005; ENSRNOP00000011005; ENSRNOG00000008176.
GeneIDi24602.
KEGGirno:24602.
UCSCiRGD:3193. rat.

Organism-specific databases

CTDi4878.
RGDi3193. Nppa.

Phylogenomic databases

eggNOGiENOG410IW5F. Eukaryota.
ENOG410ZUY6. LUCA.
GeneTreeiENSGT00510000048659.
HOGENOMiHOG000033937.
HOVERGENiHBG004227.
InParanoidiP01161.
KOiK12334.
OMAiGCNSFRY.
OrthoDBiEOG091G0SV3.
PhylomeDBiP01161.
TreeFamiTF106304.

Enzyme and pathway databases

ReactomeiR-RNO-5578768. Physiological factors.

Miscellaneous databases

EvolutionaryTraceiP01161.
PMAP-CutDBP01161.
PROiP01161.

Gene expression databases

BgeeiENSRNOG00000008176.
ExpressionAtlasiP01161. baseline and differential.
GenevisibleiP01161. RN.

Family and domain databases

InterProiIPR000663. Natr_peptide.
IPR030480. Natr_peptide_CS.
IPR002407. Natriuretic_peptide_atrial.
[Graphical view]
PfamiPF00212. ANP. 1 hit.
[Graphical view]
PRINTSiPR00711. ANATPEPTIDE.
PR00710. NATPEPTIDES.
SMARTiSM00183. NAT_PEP. 1 hit.
[Graphical view]
PROSITEiPS00263. NATRIURETIC_PEPTIDE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiANF_RAT
AccessioniPrimary (citable) accession number: P01161
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: November 2, 2016
This is version 143 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.