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Protein

Natriuretic peptides A

Gene

Nppa

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Hormone playing a key role in cardiovascular homeostasis through regulation of natriuresis, diuresis, and vasodilation. Also plays a role in female pregnancy by promoting trophoblast invasion and spiral artery remodeling in uterus. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3 (By similarity).By similarity

GO - Molecular functioni

  • hormone activity Source: RGD
  • peptide hormone receptor binding Source: RGD
  • receptor binding Source: RGD

GO - Biological processi

  • cardiac muscle hypertrophy in response to stress Source: Ensembl
  • cell growth involved in cardiac muscle cell development Source: RGD
  • cellular response to mechanical stimulus Source: RGD
  • cGMP biosynthetic process Source: RGD
  • female pregnancy Source: UniProtKB
  • heart development Source: UniProtKB
  • multicellular organismal water homeostasis Source: RGD
  • negative regulation of cell growth Source: RGD
  • negative regulation of collecting lymphatic vessel constriction Source: RGD
  • negative regulation of systemic arterial blood pressure Source: RGD
  • neuropeptide signaling pathway Source: Ensembl
  • positive regulation of cardiac muscle contraction Source: Ensembl
  • positive regulation of delayed rectifier potassium channel activity Source: BHF-UCL
  • positive regulation of heart rate Source: Ensembl
  • positive regulation of potassium ion export across plasma membrane Source: BHF-UCL
  • receptor guanylyl cyclase signaling pathway Source: UniProtKB
  • regulation of atrial cardiac muscle cell membrane repolarization Source: Ensembl
  • regulation of blood pressure Source: UniProtKB
  • regulation of blood vessel size Source: UniProtKB-KW
  • regulation of body fluid levels Source: RGD
  • regulation of calcium ion transmembrane transport via high voltage-gated calcium channel Source: BHF-UCL
  • regulation of high voltage-gated calcium channel activity Source: BHF-UCL
  • response to hypoxia Source: RGD
  • response to insulin Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Hormone, Vasoactive

Enzyme and pathway databases

ReactomeiR-RNO-5578768. Physiological factors.

Names & Taxonomyi

Protein namesi
Recommended name:
Natriuretic peptides A
Alternative name(s):
Prepronatriodilatin
Cleaved into the following 6 chains:
Alternative name(s):
Atrial natriuretic peptide
Short name:
ANP
Alternative name(s):
Atriopeptin I
Alternative name(s):
Atriopeptin II
Alternative name(s):
Atriopeptin III
Gene namesi
Name:Nppa
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 5

Organism-specific databases

RGDi3193. Nppa.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: RGD
  • extracellular space Source: Ensembl
  • mast cell granule Source: RGD
  • nucleus Source: RGD
  • perinuclear region of cytoplasm Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL4287.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 24241 PublicationAdd
BLAST
Propeptidei25 – 12298CuratedPRO_0000001505Add
BLAST
Peptidei123 – 15028Atrial natriuretic factorBy similarityPRO_0000391785Add
BLAST
Peptidei126 – 15025Auriculin-B1 PublicationPRO_0000001506Add
BLAST
Peptidei126 – 14924Auriculin-A1 PublicationPRO_0000001507Add
BLAST
Peptidei127 – 15024Atriopeptin-31 PublicationPRO_0000001508Add
BLAST
Peptidei127 – 14923Atriopeptin-21 PublicationPRO_0000001509Add
BLAST
Peptidei127 – 14721Atriopeptin-11 PublicationPRO_0000001510Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi129 ↔ 1451 Publication

Post-translational modificationi

Cleaved by CORIN upon secretion to produce the functional hormone (By similarity).By similarity
Atrial natriuretic factor: Cleaved by MME. The cleavage initiates degradation of the factor and thereby regulate its activity.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei122 – 1232Cleavage; by CORINBy similarity
Sitei129 – 1302Cleavage; by MME1 Publication

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond

Proteomic databases

PaxDbiP01161.
PRIDEiP01161.

PTM databases

iPTMnetiP01161.
PhosphoSiteiP01161.

Miscellaneous databases

PMAP-CutDBP01161.

Expressioni

Gene expression databases

ExpressionAtlasiP01161. baseline and differential.
GenevisibleiP01161. RN.

Interactioni

Subunit structurei

Interacts with NPR1.1 Publication

GO - Molecular functioni

  • hormone activity Source: RGD
  • peptide hormone receptor binding Source: RGD
  • receptor binding Source: RGD

Protein-protein interaction databases

IntActiP01161. 1 interaction.
STRINGi10116.ENSRNOP00000011005.

Chemistry

BindingDBiP01161.

Structurei

Secondary structure

1
152
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni136 – 1394Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1T34X-ray2.95H129-149[»]
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP01161.

Family & Domainsi

Sequence similaritiesi

Belongs to the natriuretic peptide family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IW5F. Eukaryota.
ENOG410ZUY6. LUCA.
GeneTreeiENSGT00510000048659.
HOGENOMiHOG000033937.
HOVERGENiHBG004227.
InParanoidiP01161.
KOiK12334.
OMAiGCNSFRY.
OrthoDBiEOG7K9K4Q.
PhylomeDBiP01161.
TreeFamiTF106304.

Family and domain databases

InterProiIPR000663. Natr_peptide.
IPR030480. Natr_peptide_CS.
IPR002407. Natriuretic_peptide_atrial.
[Graphical view]
PfamiPF00212. ANP. 1 hit.
[Graphical view]
PRINTSiPR00711. ANATPEPTIDE.
PR00710. NATPEPTIDES.
SMARTiSM00183. NAT_PEP. 1 hit.
[Graphical view]
PROSITEiPS00263. NATRIURETIC_PEPTIDE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P01161-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGSFSITKGF FLFLAFWLPG HIGANPVYSA VSNTDLMDFK NLLDHLEEKM
60 70 80 90 100
PVEDEVMPPQ ALSEQTDEAG AALSSLSEVP PWTGEVNPSQ RDGGALGRGP
110 120 130 140 150
WDPSDRSALL KSKLRALLAG PRSLRRSSCF GGRIDRIGAQ SGLGCNSFRY

RR
Length:152
Mass (Da):16,556
Last modified:July 21, 1986 - v1
Checksum:iD8AAB2191E29519F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M15868 mRNA. Translation: AAA40736.1.
X00665 mRNA. Translation: CAA25285.1.
K02062 Genomic DNA. Translation: AAA40735.1.
X01118 mRNA. Translation: CAA25586.1.
M27498 mRNA. Translation: AAA40737.1.
PIRiA22570. AWRT.
RefSeqiNP_036744.1. NM_012612.2.
UniGeneiRn.2004.

Genome annotation databases

EnsembliENSRNOT00000011005; ENSRNOP00000011005; ENSRNOG00000008176.
GeneIDi24602.
KEGGirno:24602.
UCSCiRGD:3193. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M15868 mRNA. Translation: AAA40736.1.
X00665 mRNA. Translation: CAA25285.1.
K02062 Genomic DNA. Translation: AAA40735.1.
X01118 mRNA. Translation: CAA25586.1.
M27498 mRNA. Translation: AAA40737.1.
PIRiA22570. AWRT.
RefSeqiNP_036744.1. NM_012612.2.
UniGeneiRn.2004.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1T34X-ray2.95H129-149[»]
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP01161. 1 interaction.
STRINGi10116.ENSRNOP00000011005.

Chemistry

BindingDBiP01161.
ChEMBLiCHEMBL4287.

PTM databases

iPTMnetiP01161.
PhosphoSiteiP01161.

Proteomic databases

PaxDbiP01161.
PRIDEiP01161.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000011005; ENSRNOP00000011005; ENSRNOG00000008176.
GeneIDi24602.
KEGGirno:24602.
UCSCiRGD:3193. rat.

Organism-specific databases

CTDi4878.
RGDi3193. Nppa.

Phylogenomic databases

eggNOGiENOG410IW5F. Eukaryota.
ENOG410ZUY6. LUCA.
GeneTreeiENSGT00510000048659.
HOGENOMiHOG000033937.
HOVERGENiHBG004227.
InParanoidiP01161.
KOiK12334.
OMAiGCNSFRY.
OrthoDBiEOG7K9K4Q.
PhylomeDBiP01161.
TreeFamiTF106304.

Enzyme and pathway databases

ReactomeiR-RNO-5578768. Physiological factors.

Miscellaneous databases

EvolutionaryTraceiP01161.
PMAP-CutDBP01161.
PROiP01161.

Gene expression databases

ExpressionAtlasiP01161. baseline and differential.
GenevisibleiP01161. RN.

Family and domain databases

InterProiIPR000663. Natr_peptide.
IPR030480. Natr_peptide_CS.
IPR002407. Natriuretic_peptide_atrial.
[Graphical view]
PfamiPF00212. ANP. 1 hit.
[Graphical view]
PRINTSiPR00711. ANATPEPTIDE.
PR00710. NATPEPTIDES.
SMARTiSM00183. NAT_PEP. 1 hit.
[Graphical view]
PROSITEiPS00263. NATRIURETIC_PEPTIDE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and sequence analysis of the cDNA for the rat atrial natriuretic factor precursor."
    Yamanaka M., Greenberg B., Johnson L., Seilhamer J.J., Brewer M., Friedemann T., Miller J., Atlas S.A., Laragh J., Lewicki J., Fiddes J.C.
    Nature 309:719-722(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Structure of rat atrial natriuretic factor precursor deduced from cDNA sequence."
    Maki M., Takayanagi R., Misono K.S., Pandey K.N., Tibbetts C., Inagami T.
    Nature 309:722-724(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "The structure of rat preproatrial natriuretic factor as defined by a complementary DNA clone."
    Seidman C.E., Duby A.D., Choi E., Graham R.M., Haber E., Homcy C., Smith J.A., Seidman J.G.
    Science 225:324-326(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. "Identification of rat gamma atrial natriuretic polypeptide and characterization of the cDNA encoding its precursor."
    Kangawa K., Tawaragi Y., Oikawa S., Mizuno A., Sakuragawa Y., Nakazato H., Fukuda A., Minamino N., Matsuo H.
    Nature 312:152-155(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  5. "The gene for rat atrial natriuretic factor."
    Argentin S., Nemer M., Drouin J., Scott G.K., Kennedy B.P., Davies P.L.
    J. Biol. Chem. 260:4568-4571(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  6. "Molecular cloning and characterization of DNA sequences encoding rat and human atrial natriuretic factors."
    Zivin R.A., Condra J.H., Dixon R.A.F., Seidah N.G., Chretien M., Nemer M., Chamberland M., Drouin J.
    Proc. Natl. Acad. Sci. U.S.A. 81:6325-6329(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  7. "The elucidation of the structure of atrial natriuretic factor, a new peptide hormone."
    Flynn T.G.
    Can. J. Physiol. Pharmacol. 65:2013-2020(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  8. "Identification of atrial natriuretic factor gene transcripts in the central nervous system of the rat."
    Gardner D.G., Vlasuk G.P., Baxter J.D., Fiddes J.C., Lewicki J.A.
    Proc. Natl. Acad. Sci. U.S.A. 84:2175-2179(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 38-152.
  9. "Purification, sequencing and synthesis of natriuretic and vasoactive rat atrial peptide."
    Atlas S.A., Kleinert H.D., Camargo M.J., Januszewicz A., Sealey J.E., Laragh J.H., Schilling J.W., Lewicki J.A., Johnson L.K., Maack T.
    Nature 309:717-719(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 126-150, SYNTHESIS OF 126-149.
  10. "Purification and sequence analysis of bioactive atrial peptides (atriopeptins)."
    Currie M.G., Geller D.M., Cole B.R., Siegel N.R., Fok K.F., Adams S.P., Eubanks S.R., Galluppi G.R., Needleman P.
    Science 223:67-69(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 127-149, SYNTHESIS.
  11. Cited for: PROTEIN SEQUENCE OF 118-150.
  12. "NH2-terminal fragment of rat pro-atrial natriuretic factor in the circulation: identification, radioimmunoassay and half-life."
    Thibault G., Murthy K.K., Gutkowska J., Seidah N.G., Lazure C., Chretien M., Cantin M.
    Peptides 9:47-53(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 25-38.
  13. "Identification of protease 3.4.24.11 as the major atrial natriuretic factor degrading enzyme in the rat kidney."
    Sonnenberg J.L., Sakane Y., Jeng A.Y., Koehn J.A., Ansell J.A., Wennogle L.P., Ghai R.D.
    Peptides 9:173-180(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEOLYTIC PROCESSING BY MME, CLEAVAGE SITE.
  14. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  15. "Crystal structure of hormone-bound atrial natriuretic peptide receptor extracellular domain: rotation mechanism for transmembrane signal transduction."
    Ogawa H., Qiu Y., Ogata C.M., Misono K.S.
    J. Biol. Chem. 279:28625-28631(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.95 ANGSTROMS) OF 129-149 IN COMPLEX WITH NPR1, DISULFIDE BOND.

Entry informationi

Entry nameiANF_RAT
AccessioniPrimary (citable) accession number: P01161
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: June 8, 2016
This is version 141 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.