P01139 (NGF_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-nerve growth factor
Short name=Beta-NGF

Gene names

Name:	Ngf
Synonyms:	Ngfb

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	241 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades through those receptor tyrosine kinase to regulate neuronal proliferation, differentiation and survival.
Subunit structure	Homodimer.
Subcellular location	Secreted.
Sequence similarities	Belongs to the NGF-beta family.
Sequence caution	The sequence AAA37687.1 differs from that shown. Reason: Erroneous initiation. The sequence AAA39818.1 differs from that shown. Reason: Erroneous initiation. The sequence AAA39820.1 differs from that shown. Reason: Erroneous initiation. The sequence AAA39821.1 differs from that shown. Reason: Erroneous initiation. The sequence CAA24221.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Growth factor
PTM	Cleavage on pair of basic residues Disulfide bond Glycoprotein Phosphoprotein
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	negative regulation of neuron apoptosis Inferred from genetic interaction. Source: MGI peripheral nervous system development Inferred from mutant phenotype. Source: MGI positive regulation of axon extension Inferred from mutant phenotype. Source: MGI regulation of neurotransmitter secretion Inferred from genetic interaction. Source: MGI sensory perception of pain Inferred from mutant phenotype. Source: MGI
Cellular component	endoplasmic reticulum lumen Traceable author statement. Source: Reactome extracellular region Traceable author statement. Source: Reactome
Molecular function	growth factor activity Inferred from electronic annotation. Source: UniProtKB-KW receptor signaling protein activity Inferred from direct assay. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 18

18

Potential

Propeptide

19 – 121

103

PRO_0000019601

Chain

122 – 241

120

Beta-nerve growth factor

PRO_0000019602

Amino acid modifications

Modified residue

84

1

Phosphoserine By similarity

Glycosylation

69

1

N-linked (GlcNAc...) Potential

Glycosylation

114

1

N-linked (GlcNAc...) Potential

Disulfide bond

136 ↔ 201

Disulfide bond

179 ↔ 229

Disulfide bond

189 ↔ 231

Experimental info

Sequence conflict

233 – 241

9

LSRKATRRG → CSAGRLQEEADLPAAPFPTC PLHTLLGPSLPQPVNYFKL in AAB26820. Ref.5

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

241

Helix Strand Turn

Details...

Beta strand

133 – 136

4

Beta strand

138 – 144

7

Beta strand

148 – 151

4

Beta strand

156 – 159

4

Beta strand

161 – 165

5

Beta strand

168 – 171

4

Beta strand

174 – 180

7

Turn

194 – 196

3

Beta strand

197 – 213

17

Beta strand

218 – 235

18

Sequences

	Sequence		Length	Mass (Da)
	P01139 [UniParc]. Last modified January 1, 1990. Version 2. Checksum: 164465E1DC550081	FASTA	241	27,077
	10 20 30 40 50 60 MSMLFYTLIT AFLIGVQAEP YTDSNVPEGD SVPEAHWTKL QHSLDTALRR ARSAPTAPIA 70 80 90 100 110 120 ARVTGQTRNI TVDPRLFKKR RLHSPRVLFS TQPPPTSSDT LDLDFQAHGT IPFNRTHRSK 130 140 150 160 170 180 RSSTHPVFHM GEFSVCDSVS VWVGDKTTAT DIKGKEVTVL AEVNINNSVF RQYFFETKCR 190 200 210 220 230 240 ASNPVESGCR GIDSKHWNSY CTTTHTFVKA LTTDEKQAAW RFIRIDTACV CVLSRKATRR G

References

[1]	"Isolation and nucleotide sequence of a cDNA encoding the precursor of mouse nerve growth factor." Scott J., Selby M.J., Urdea M.S., Quiroga M., Bell G.I., Rutter W.J. Nature 302:538-540(1983) [PubMed: 6336309] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Submandibular gland.
[2]	"Human beta-nerve growth factor gene sequence highly homologous to that of mouse." Ullrich A., Gray A., Berman C., Dull T.J. Nature 303:821-825(1983) [PubMed: 6688123] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Sequence homology of human and mouse beta-NGF subunit genes." Ullrich A., Gray A., Berman C., Coussens L., Dull T.J. Cold Spring Harb. Symp. Quant. Biol. 48:435-442(1983) [PubMed: 6327169] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]	"Mouse nerve growth factor gene: structure and expression." Selby M.J., Edwards R., Sharp F., Rutter W.J. Mol. Cell. Biol. 7:3057-3064(1987) [PubMed: 3670305] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: C57BL/6. Tissue: Submandibular gland.
[5]	"Production and secretion of nerve growth factor by clonal striated muscle cell line, G8-1." Yamamoto T., Yamakuni T., Okabe N., Amano T. Neurochem. Int. 21:251-258(1992) [PubMed: 1284621] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Skeletal muscle.
[6]	"Amino acid sequences of mouse 2.5S nerve growth factor. II. Isolation and characterization of the thermolytic and peptic peptides and the complete covalent structure." Angeletti R.H., Hermodson M.A., Bradshaw R.A. Biochemistry 12:100-115(1973) [PubMed: 4566923] [Abstract] Cited for: PROTEIN SEQUENCE OF 122-239.
[7]	"New protein fold revealed by a 2.3-A resolution crystal structure of nerve growth factor." McDonald N.Q., Lapatto R., Murray-Rust J., Gunning J., Wlodawer A., Blundell T.L. Nature 354:411-414(1991) [PubMed: 1956407] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS).
[8]	"Nerve growth factor in different crystal forms displays structural flexibility and reveals zinc binding sites." Holland D.R., Cousens L.S., Meng W., Matthews B.W. J. Mol. Biol. 239:385-400(1994) [PubMed: 8201620] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS).
[9]	"Structure of mouse 7S NGF: a complex of nerve growth factor with four binding proteins." Bax B., Blundell T.L., Murray-Rust J., McDonald N.Q. Structure 5:1275-1285(1997) [PubMed: 9351801] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.15 ANGSTROMS) OF 7S COMPLEX. Strain: Swiss Webster. Tissue: Submandibular gland.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M35075 mRNA. Translation: AAA39818.1. Different initiation.
V00836 mRNA. Translation: CAA24221.1. Different initiation.
K01759 mRNA. Translation: AAA39820.1. Different initiation.
M14805 mRNA. Translation: AAA39821.1. Different initiation.
M17298, M17296, M17297 Genomic DNA. Translation: AAA37687.1. Different initiation.
S62089 mRNA. Translation: AAB26820.2.

IPI

IPI00882237.

RefSeq

NP_001106168.1. NM_001112698.1.
NP_038637.1. NM_013609.2.

UniGene

Mm.1259.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1BET	X-ray	2.30	A	131-237	[»]
1BTG	X-ray	2.50	A/B/C	130-239	[»]
1SGF	X-ray	3.15	B/Y	122-239	[»]
3IJ2	X-ray	3.75	A/B	19-238	[»]

ProteinModelPortal

P01139.

SMR

P01139. Positions 131-237.

ModBase

Search...

Protein-protein interaction databases

STRING

P01139.

Proteomic databases

PRIDE

P01139.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSMUST00000035952; ENSMUSP00000040345; ENSMUSG00000027859.

GeneID

18049.

KEGG

mmu:18049.

Organism-specific databases

CTD

4803.

MGI

MGI:97321. Ngf.

Phylogenomic databases

eggNOG

maNOG12976.

HOVERGEN

HBG006494.

InParanoid

P01139.

OrthoDB

EOG4BRWMZ.

Enzyme and pathway databases

Reactome

REACT_115202. Signal Transduction.

Gene expression databases

ArrayExpress

P01139.

Bgee

P01139.

CleanEx

MM_NGF.

Genevestigator

P01139.

GermOnline

ENSMUSG00000027859. Mus musculus.

Family and domain databases

InterPro

IPR020408. Nerve_growth_factor-like.
IPR002072. Nerve_growth_factor-rel.
IPR020425. Nerve_growth_factor_bsu.
IPR020437. Nerve_growth_factor_bsu_mml.
IPR019846. Nerve_growth_factor_CS.
[Graphical view]

KO

K02582.

PANTHER

PTHR11589. NGF. 1 hit.

Pfam

PF00243. NGF. 1 hit.
[Graphical view]

PIRSF

PIRSF001789. NGF. 1 hit.

PRINTS

PR01925. MAMLNGFBETA.
PR00268. NGF.
PR01913. NGFBETA.

ProDom

PD002052. Nerve_growth_factor-rel. 1 hit.
[Graphical view] [Entries sharing at least one domain]

SMART

SM00140. NGF. 1 hit.
[Graphical view]

PROSITE

PS00248. NGF_1. 1 hit.
PS50270. NGF_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

293175.

SOURCE

Search...

Entry information

Entry name

NGF_MOUSE

Accession

Primary (citable) accession number: P01139
Secondary accession number(s): Q63864, Q6LDB7

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	January 1, 1990
Last modified:	January 25, 2012
This is version 114 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program