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P01139

- NGF_MOUSE

UniProt

P01139 - NGF_MOUSE

Protein

Beta-nerve growth factor

Gene

Ngf

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 139 (01 Oct 2014)
      Sequence version 2 (01 Jan 1990)
      Previous versions | rss
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    Functioni

    Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades through those receptor tyrosine kinase to regulate neuronal proliferation, differentiation and survival.

    GO - Molecular functioni

    1. receptor signaling protein activity Source: MGI

    GO - Biological processi

    1. circadian rhythm Source: UniProtKB
    2. extrinsic apoptotic signaling pathway in absence of ligand Source: MGI
    3. intracellular signal transduction Source: GOC
    4. negative regulation of neuron apoptotic process Source: MGI
    5. neuron apoptotic process Source: MGI
    6. neuron projection morphogenesis Source: MGI
    7. peripheral nervous system development Source: MGI
    8. positive regulation of axon extension Source: MGI
    9. positive regulation of neuron maturation Source: MGI
    10. positive regulation of neuron projection development Source: MGI
    11. positive regulation of neurotrophin TRK receptor signaling pathway Source: MGI
    12. positive regulation of protein autophosphorylation Source: MGI
    13. positive regulation of sequence-specific DNA binding transcription factor activity Source: MGI
    14. regulation of neuron differentiation Source: MGI
    15. regulation of neurotransmitter secretion Source: MGI
    16. sensory perception of pain Source: MGI

    Keywords - Molecular functioni

    Growth factor

    Enzyme and pathway databases

    ReactomeiREACT_202086. p75NTR negatively regulates cell cycle via SC1.
    REACT_202271. NRIF signals cell death from the nucleus.
    REACT_202778. Retrograde neurotrophin signalling.
    REACT_205300. PI3K/AKT activation.
    REACT_206713. NGF processing.
    REACT_207546. NFG and proNGF binds to p75NTR.
    REACT_209641. NRAGE signals death through JNK.
    REACT_210754. NADE modulates death signalling.
    REACT_212952. Signalling to STAT3.
    REACT_213333. TRKA activation by NGF.
    REACT_214242. Axonal growth stimulation.
    REACT_214670. p75NTR recruits signalling complexes.
    REACT_217312. Ceramide signalling.
    REACT_218887. NF-kB is activated and signals survival.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-nerve growth factor
    Short name:
    Beta-NGF
    Gene namesi
    Name:Ngf
    Synonyms:Ngfb
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 3

    Organism-specific databases

    MGIiMGI:97321. Ngf.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum lumen Source: Reactome
    2. extracellular region Source: Reactome

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1818Sequence AnalysisAdd
    BLAST
    Propeptidei19 – 1211031 PublicationPRO_0000019601Add
    BLAST
    Chaini122 – 241120Beta-nerve growth factorPRO_0000019602Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi69 – 691N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi114 – 1141N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi136 ↔ 201
    Disulfide bondi179 ↔ 229
    Disulfide bondi189 ↔ 231

    Keywords - PTMi

    Cleavage on pair of basic residues, Disulfide bond, Glycoprotein

    Proteomic databases

    MaxQBiP01139.
    PRIDEiP01139.

    PTM databases

    PhosphoSiteiP01139.

    Expressioni

    Inductioni

    Expression oscillates in a circadian manner in the suprachiasmatic nucleus (SCN) of the brain.1 Publication

    Gene expression databases

    BgeeiP01139.
    CleanExiMM_NGF.
    GenevestigatoriP01139.

    Interactioni

    Subunit structurei

    Homodimer.

    Protein-protein interaction databases

    BioGridi201764. 1 interaction.
    DIPiDIP-59840N.

    Structurei

    Secondary structure

    1
    241
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi133 – 1364
    Beta strandi138 – 1447
    Beta strandi148 – 1514
    Beta strandi156 – 1594
    Beta strandi161 – 1655
    Beta strandi168 – 1714
    Beta strandi174 – 1807
    Turni194 – 1963
    Beta strandi197 – 21317
    Beta strandi218 – 23518

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1BETX-ray2.30A131-237[»]
    1BTGX-ray2.50A/B/C130-239[»]
    1SGFX-ray3.15B/Y122-239[»]
    3IJ2X-ray3.75A/B19-241[»]
    4EAXX-ray2.30A/B/C/D122-241[»]
    ProteinModelPortaliP01139.
    SMRiP01139. Positions 131-238.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP01139.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the NGF-beta family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG44820.
    GeneTreeiENSGT00390000007725.
    HOVERGENiHBG006494.
    InParanoidiP01139.
    KOiK02582.
    PhylomeDBiP01139.

    Family and domain databases

    Gene3Di2.10.90.10. 1 hit.
    InterProiIPR029034. Cystine-knot_cytokine.
    IPR020408. Nerve_growth_factor-like.
    IPR002072. Nerve_growth_factor-rel.
    IPR020425. Nerve_growth_factor_bsu.
    IPR020437. Nerve_growth_factor_bsu_mml.
    IPR019846. Nerve_growth_factor_CS.
    [Graphical view]
    PANTHERiPTHR11589. PTHR11589. 1 hit.
    PfamiPF00243. NGF. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001789. NGF. 1 hit.
    PRINTSiPR01925. MAMLNGFBETA.
    PR00268. NGF.
    PR01913. NGFBETA.
    ProDomiPD002052. Nerve_growth_factor-rel. 1 hit.
    [Graphical view] [Entries sharing at least one domain]
    SMARTiSM00140. NGF. 1 hit.
    [Graphical view]
    SUPFAMiSSF57501. SSF57501. 1 hit.
    PROSITEiPS00248. NGF_1. 1 hit.
    PS50270. NGF_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P01139-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSMLFYTLIT AFLIGVQAEP YTDSNVPEGD SVPEAHWTKL QHSLDTALRR    50
    ARSAPTAPIA ARVTGQTRNI TVDPRLFKKR RLHSPRVLFS TQPPPTSSDT 100
    LDLDFQAHGT IPFNRTHRSK RSSTHPVFHM GEFSVCDSVS VWVGDKTTAT 150
    DIKGKEVTVL AEVNINNSVF RQYFFETKCR ASNPVESGCR GIDSKHWNSY 200
    CTTTHTFVKA LTTDEKQAAW RFIRIDTACV CVLSRKATRR G 241
    Length:241
    Mass (Da):27,077
    Last modified:January 1, 1990 - v2
    Checksum:i164465E1DC550081
    GO

    Sequence cautioni

    The sequence AAA37687.1 differs from that shown. Reason: Erroneous initiation.
    The sequence AAA39818.1 differs from that shown. Reason: Erroneous initiation.
    The sequence AAA39820.1 differs from that shown. Reason: Erroneous initiation.
    The sequence AAA39821.1 differs from that shown. Reason: Erroneous initiation.
    The sequence CAA24221.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti233 – 2419LSRKATRRG → CSAGRLQEEADLPAAPFPTC PLHTLLGPSLPQPVNYFKL in AAB26820. (PubMed:1284621)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M35075 mRNA. Translation: AAA39818.1. Different initiation.
    V00836 mRNA. Translation: CAA24221.1. Different initiation.
    K01759 mRNA. Translation: AAA39820.1. Different initiation.
    M14805 mRNA. Translation: AAA39821.1. Different initiation.
    M17298, M17296, M17297 Genomic DNA. Translation: AAA37687.1. Different initiation.
    S62089 mRNA. Translation: AAB26820.2.
    CCDSiCCDS51025.1.
    RefSeqiNP_001106168.1. NM_001112698.2.
    NP_038637.1. NM_013609.3.
    XP_006501171.1. XM_006501108.1.
    XP_006501172.1. XM_006501109.1.
    XP_006501173.1. XM_006501110.1.
    UniGeneiMm.1259.

    Genome annotation databases

    EnsembliENSMUST00000035952; ENSMUSP00000040345; ENSMUSG00000027859.
    GeneIDi18049.
    KEGGimmu:18049.
    UCSCiuc012cuz.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M35075 mRNA. Translation: AAA39818.1 . Different initiation.
    V00836 mRNA. Translation: CAA24221.1 . Different initiation.
    K01759 mRNA. Translation: AAA39820.1 . Different initiation.
    M14805 mRNA. Translation: AAA39821.1 . Different initiation.
    M17298 , M17296 , M17297 Genomic DNA. Translation: AAA37687.1 . Different initiation.
    S62089 mRNA. Translation: AAB26820.2 .
    CCDSi CCDS51025.1.
    RefSeqi NP_001106168.1. NM_001112698.2.
    NP_038637.1. NM_013609.3.
    XP_006501171.1. XM_006501108.1.
    XP_006501172.1. XM_006501109.1.
    XP_006501173.1. XM_006501110.1.
    UniGenei Mm.1259.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1BET X-ray 2.30 A 131-237 [» ]
    1BTG X-ray 2.50 A/B/C 130-239 [» ]
    1SGF X-ray 3.15 B/Y 122-239 [» ]
    3IJ2 X-ray 3.75 A/B 19-241 [» ]
    4EAX X-ray 2.30 A/B/C/D 122-241 [» ]
    ProteinModelPortali P01139.
    SMRi P01139. Positions 131-238.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 201764. 1 interaction.
    DIPi DIP-59840N.

    PTM databases

    PhosphoSitei P01139.

    Proteomic databases

    MaxQBi P01139.
    PRIDEi P01139.

    Protocols and materials databases

    DNASUi 18049.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000035952 ; ENSMUSP00000040345 ; ENSMUSG00000027859 .
    GeneIDi 18049.
    KEGGi mmu:18049.
    UCSCi uc012cuz.1. mouse.

    Organism-specific databases

    CTDi 4803.
    MGIi MGI:97321. Ngf.

    Phylogenomic databases

    eggNOGi NOG44820.
    GeneTreei ENSGT00390000007725.
    HOVERGENi HBG006494.
    InParanoidi P01139.
    KOi K02582.
    PhylomeDBi P01139.

    Enzyme and pathway databases

    Reactomei REACT_202086. p75NTR negatively regulates cell cycle via SC1.
    REACT_202271. NRIF signals cell death from the nucleus.
    REACT_202778. Retrograde neurotrophin signalling.
    REACT_205300. PI3K/AKT activation.
    REACT_206713. NGF processing.
    REACT_207546. NFG and proNGF binds to p75NTR.
    REACT_209641. NRAGE signals death through JNK.
    REACT_210754. NADE modulates death signalling.
    REACT_212952. Signalling to STAT3.
    REACT_213333. TRKA activation by NGF.
    REACT_214242. Axonal growth stimulation.
    REACT_214670. p75NTR recruits signalling complexes.
    REACT_217312. Ceramide signalling.
    REACT_218887. NF-kB is activated and signals survival.

    Miscellaneous databases

    EvolutionaryTracei P01139.
    NextBioi 293175.
    PROi P01139.
    SOURCEi Search...

    Gene expression databases

    Bgeei P01139.
    CleanExi MM_NGF.
    Genevestigatori P01139.

    Family and domain databases

    Gene3Di 2.10.90.10. 1 hit.
    InterProi IPR029034. Cystine-knot_cytokine.
    IPR020408. Nerve_growth_factor-like.
    IPR002072. Nerve_growth_factor-rel.
    IPR020425. Nerve_growth_factor_bsu.
    IPR020437. Nerve_growth_factor_bsu_mml.
    IPR019846. Nerve_growth_factor_CS.
    [Graphical view ]
    PANTHERi PTHR11589. PTHR11589. 1 hit.
    Pfami PF00243. NGF. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001789. NGF. 1 hit.
    PRINTSi PR01925. MAMLNGFBETA.
    PR00268. NGF.
    PR01913. NGFBETA.
    ProDomi PD002052. Nerve_growth_factor-rel. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    SMARTi SM00140. NGF. 1 hit.
    [Graphical view ]
    SUPFAMi SSF57501. SSF57501. 1 hit.
    PROSITEi PS00248. NGF_1. 1 hit.
    PS50270. NGF_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation and nucleotide sequence of a cDNA encoding the precursor of mouse nerve growth factor."
      Scott J., Selby M.J., Urdea M.S., Quiroga M., Bell G.I., Rutter W.J.
      Nature 302:538-540(1983) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Submandibular gland.
    2. "Human beta-nerve growth factor gene sequence highly homologous to that of mouse."
      Ullrich A., Gray A., Berman C., Dull T.J.
      Nature 303:821-825(1983) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. "Sequence homology of human and mouse beta-NGF subunit genes."
      Ullrich A., Gray A., Berman C., Coussens L., Dull T.J.
      Cold Spring Harb. Symp. Quant. Biol. 48:435-442(1983) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    4. "Mouse nerve growth factor gene: structure and expression."
      Selby M.J., Edwards R., Sharp F., Rutter W.J.
      Mol. Cell. Biol. 7:3057-3064(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: C57BL/6.
      Tissue: Submandibular gland.
    5. "Production and secretion of nerve growth factor by clonal striated muscle cell line, G8-1."
      Yamamoto T., Yamakuni T., Okabe N., Amano T.
      Neurochem. Int. 21:251-258(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Skeletal muscle.
    6. "Amino acid sequences of mouse 2.5S nerve growth factor. II. Isolation and characterization of the thermolytic and peptic peptides and the complete covalent structure."
      Angeletti R.H., Hermodson M.A., Bradshaw R.A.
      Biochemistry 12:100-115(1973) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 122-239.
    7. "p75 neurotrophin receptor is a clock gene that regulates oscillatory components of circadian and metabolic networks."
      Baeza-Raja B., Eckel-Mahan K., Zhang L., Vagena E., Tsigelny I.F., Sassone-Corsi P., Ptacek L.J., Akassoglou K.
      J. Neurosci. 33:10221-10234(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
    8. "New protein fold revealed by a 2.3-A resolution crystal structure of nerve growth factor."
      McDonald N.Q., Lapatto R., Murray-Rust J., Gunning J., Wlodawer A., Blundell T.L.
      Nature 354:411-414(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS).
    9. "Nerve growth factor in different crystal forms displays structural flexibility and reveals zinc binding sites."
      Holland D.R., Cousens L.S., Meng W., Matthews B.W.
      J. Mol. Biol. 239:385-400(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS).
    10. "Structure of mouse 7S NGF: a complex of nerve growth factor with four binding proteins."
      Bax B., Blundell T.L., Murray-Rust J., McDonald N.Q.
      Structure 5:1275-1285(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (3.15 ANGSTROMS) OF 7S COMPLEX.
      Strain: Swiss Webster.
      Tissue: Submandibular gland.

    Entry informationi

    Entry nameiNGF_MOUSE
    AccessioniPrimary (citable) accession number: P01139
    Secondary accession number(s): Q63864, Q6LDB7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: January 1, 1990
    Last modified: October 1, 2014
    This is version 139 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3