Beta-nerve growth factor precursor - Mus musculus (Mouse)

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Reviewed, UniProtKB/Swiss-Prot P01139 (NGF_MOUSE)

Last modified June 16, 2009. Version 95. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
Beta-nerve growth factor
Short name=Beta-NGF

Gene names

Name:	Ngf
Synonyms:	Ngfb

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

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Protein attributes

Sequence length	241 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. It stimulates division and differentiation of sympathetic and embryonic sensory neurons.
Subunit structure	Homodimer.
Subcellular location	Secreted.
Sequence similarities	Belongs to the NGF-beta family.

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Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Growth factor
PTM	Cleavage on pair of basic residues Disulfide bond Glycoprotein Phosphoprotein
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	negative regulation of neuron apoptosis Inferred from genetic interaction. Source: MGI peripheral nervous system development Inferred from mutant phenotype. Source: MGI positive regulation of axon extension Inferred from mutant phenotype. Source: MGI regulation of neurotransmitter secretion Inferred from genetic interaction. Source: MGI sensory perception of pain Inferred from mutant phenotype. Source: MGI
Cellular component	endoplasmic reticulum lumen Inferred from Experiment. Source: Reactome extracellular region Inferred from Experiment. Source: Reactome
Molecular function	growth factor activity Inferred from electronic annotation. Source: UniProtKB-KW receptor signaling protein activity Inferred from direct assay. Source: MGI
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 18

Potential

Propeptide

19 – 121

103

Ref.6

PRO_0000019601

Chain

122 – 241

120

Beta-nerve growth factor

PRO_0000019602

Amino acid modifications

Modified residue

Phosphoserine By similarity

Glycosylation

N-linked (GlcNAc...) Potential

Glycosylation

114

N-linked (GlcNAc...) Potential

Disulfide bond

136 ↔ 201

Disulfide bond

179 ↔ 229

Disulfide bond

189 ↔ 231

Experimental info

Sequence conflict

233 – 241

LSRKATRRG → CSAGRLQEEADLPAAPFPTC PLHTLLGPSLPQPVNYFKL in AAB26820. Ref.5

Secondary structure

241

Helix Strand Turn

Details...

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Sequences

Sequence

Length

Mass (Da)

Tools

P01139-1 [UniParc].

Last modified January 1, 1990. Version 2.
Checksum: 164465E1DC550081
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FASTA

241

27,077

        10         20         30         40         50         60 
MSMLFYTLIT AFLIGVQAEP YTDSNVPEGD SVPEAHWTKL QHSLDTALRR ARSAPTAPIA 

        70         80         90        100        110        120 
ARVTGQTRNI TVDPRLFKKR RLHSPRVLFS TQPPPTSSDT LDLDFQAHGT IPFNRTHRSK 

       130        140        150        160        170        180 
RSSTHPVFHM GEFSVCDSVS VWVGDKTTAT DIKGKEVTVL AEVNINNSVF RQYFFETKCR 

       190        200        210        220        230        240 
ASNPVESGCR GIDSKHWNSY CTTTHTFVKA LTTDEKQAAW RFIRIDTACV CVLSRKATRR 


G

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References

[1]	"Isolation and nucleotide sequence of a cDNA encoding the precursor of mouse nerve growth factor." Scott J., Selby M.J., Urdea M.S., Quiroga M., Bell G.I., Rutter W.J. Nature 302:538-540(1983) [PubMed: 6336309] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Submandibular gland.
[2]	"Human beta-nerve growth factor gene sequence highly homologous to that of mouse." Ullrich A., Gray A., Berman C., Dull T.J. Nature 303:821-825(1983) [PubMed: 6688123] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Sequence homology of human and mouse beta-NGF subunit genes." Ullrich A., Gray A., Berman C., Coussens L., Dull T.J. Cold Spring Harb. Symp. Quant. Biol. 48:435-442(1983) [PubMed: 6327169] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]	"Mouse nerve growth factor gene: structure and expression." Selby M.J., Edwards R., Sharp F., Rutter W.J. Mol. Cell. Biol. 7:3057-3064(1987) [PubMed: 3670305] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: C57BL/6. Tissue: Submandibular gland.
[5]	"Production and secretion of nerve growth factor by clonal striated muscle cell line, G8-1." Yamamoto T., Yamakuni T., Okabe N., Amano T. Neurochem. Int. 21:251-258(1992) [PubMed: 1284621] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Skeletal muscle.
[6]	"Amino acid sequences of mouse 2.5S nerve growth factor. II. Isolation and characterization of the thermolytic and peptic peptides and the complete covalent structure." Angeletti R.H., Hermodson M.A., Bradshaw R.A. Biochemistry 12:100-115(1973) [PubMed: 4566923] [Abstract] Cited for: PROTEIN SEQUENCE OF 122-239.
[7]	"New protein fold revealed by a 2.3-A resolution crystal structure of nerve growth factor." McDonald N.Q., Lapatto R., Murray-Rust J., Gunning J., Wlodawer A., Blundell T.L. Nature 354:411-414(1991) [PubMed: 1956407] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS).
[8]	"Nerve growth factor in different crystal forms displays structural flexibility and reveals zinc binding sites." Holland D.R., Cousens L.S., Meng W., Matthews B.W. J. Mol. Biol. 239:385-400(1994) [PubMed: 8201620] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS).
[9]	"Structure of mouse 7S NGF: a complex of nerve growth factor with four binding proteins." Bax B., Blundell T.L., Murray-Rust J., McDonald N.Q. Structure 5:1275-1285(1997) [PubMed: 9351801] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.15 ANGSTROMS) OF 7S COMPLEX. Strain: Swiss Webster. Tissue: Submandibular gland.
+	Additional computationally mapped references.

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Cross-references

Sequence databases

M35075 mRNA. Translation: AAA39818.1. Different initiation.
V00836 mRNA. Translation: CAA24221.1. Different initiation.
K01759 mRNA. Translation: AAA39820.1. Different initiation.
M14805 mRNA. Translation: AAA39821.1. Different initiation.
M17298, M17296, M17297 Genomic DNA. Translation: AAA37687.1. Different initiation.
S62089 mRNA. Translation: AAB26820.2.

IPI

IPI00882237.

RefSeq

NP_001106168.1.

UniGene

Mm.1259

3D structure databases

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1BET	X-ray	2.30	A	131-237	[»]
1BTG	X-ray	2.50	A/B/C	130-239	[»]
1SGF	X-ray	3.15	B/Y	122-239	[»]

ModBase

Search...

Genome annotation databases

Ensembl

ENSMUSG00000027859. Mus musculus. [Contig view]

GeneID

18049.

KEGG

mmu:18049.

Organism-specific databases

MGI

MGI:97321. Ngf.

Phylogenomic databases

HOVERGEN

P01139.

Gene expression databases

ArrayExpress

P01139.

Bgee

P01139.

CleanEx

MM_NGF.

GermOnline

ENSMUSG00000027859. Mus musculus.

Family and domain databases

InterPro

IPR002072. Nerve_growth_factor-rel.
IPR019846. Nerve_growth_factor_CS.
[Graphical view]

PANTHER

PTHR11589. NGF. 1 hit.

Pfam

PF00243. NGF. 1 hit.
[Graphical view]

PIRSF

PIRSF001789. NGF. 1 hit.

PRINTS

PR00268. NGF.

ProDom

PD002052. NGF. 2 hits.
[Graphical view] [Entries sharing at least one domain]

SMART

SM00140. NGF. 1 hit.
[Graphical view]

PROSITE

PS00248. NGF_1. 1 hit.
PS50270. NGF_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

293175.

SOURCE

Search...

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Entry information

Entry name

NGF_MOUSE

Accession

Primary (citable) accession number: P01139
Secondary accession number(s): Q63864, Q6LDB7

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	January 1, 1990
Last modified:	June 16, 2009
This is version 95 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

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Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families