Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Protransforming growth factor alpha

Gene

Tgfa

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor/EGFR and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar.

GO - Molecular functioni

  1. epidermal growth factor receptor binding Source: UniProtKB
  2. growth factor activity Source: HGNC
  3. MAP kinase kinase activity Source: Ensembl

GO - Biological processi

  1. activation of MAPK activity Source: HGNC
  2. angiogenesis Source: Ensembl
  3. epidermal growth factor receptor signaling pathway Source: RGD
  4. mammary gland alveolus development Source: Ensembl
  5. negative regulation of apoptotic process Source: RGD
  6. negative regulation of cellular process Source: RGD
  7. positive regulation of cell proliferation Source: RGD
  8. positive regulation of epidermal growth factor-activated receptor activity Source: HGNC
  9. positive regulation of epithelial cell proliferation Source: HGNC
  10. positive regulation of mitosis Source: HGNC
  11. response to drug Source: RGD
  12. wound healing Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Growth factor, Mitogen

Names & Taxonomyi

Protein namesi
Recommended name:
Protransforming growth factor alpha
Cleaved into the following chain:
Alternative name(s):
EGF-like TGF
Short name:
ETGF
TGF type 1
Gene namesi
Name:Tgfa
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 4

Organism-specific databases

RGDi3849. Tgfa.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini24 – 9774ExtracellularSequence AnalysisAdd
BLAST
Transmembranei98 – 12326HelicalSequence AnalysisAdd
BLAST
Topological domaini124 – 15936CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. basolateral plasma membrane Source: Ensembl
  2. cell surface Source: Ensembl
  3. cytoplasmic vesicle Source: Ensembl
  4. extracellular space Source: RGD
  5. integral component of plasma membrane Source: RGD
  6. nucleus Source: RGD
  7. perinuclear region of cytoplasm Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323Sequence AnalysisAdd
BLAST
Chaini24 – 159136Protransforming growth factor alphaPRO_0000302748Add
BLAST
Propeptidei24 – 3815Removed in mature formPRO_0000007764Add
BLAST
Chaini39 – 8850Transforming growth factor alphaPRO_0000007765Add
BLAST
Propeptidei89 – 15971Removed in mature formPRO_0000007766Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi25 – 251N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi46 ↔ 59PROSITE-ProRule annotation
Disulfide bondi54 ↔ 70PROSITE-ProRule annotation
Disulfide bondi72 ↔ 81PROSITE-ProRule annotation
Lipidationi152 – 1521S-palmitoyl cysteineBy similarity
Lipidationi153 – 1531S-palmitoyl cysteineBy similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate

Expressioni

Gene expression databases

GenevestigatoriP01134.

Interactioni

Subunit structurei

Interacts with the PDZ domains of MAGI3, SDCBP and SNTA1. The interaction with SDCBP, is required for the targeting to the cell surface. In the endoplasmic reticulum, in its immature form (i.e. with a prosegment and lacking full N-glycosylation), interacts with CNIH. In the Golgi apparatus, may form a complex with CNIH and GORASP2. Interacts (via cytoplasmic C-terminal domain) with NKD2 (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000054248.

Structurei

3D structure databases

ProteinModelPortaliP01134.
SMRiP01134. Positions 39-88.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini42 – 8241EGF-likePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 EGF-like domain.PROSITE-ProRule annotation

Keywords - Domaini

EGF-like domain, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG41326.
GeneTreeiENSGT00730000110951.
HOGENOMiHOG000013036.
HOVERGENiHBG000330.
InParanoidiP01134.
KOiK08774.
OMAiCHSETGC.
OrthoDBiEOG7VQJGP.
PhylomeDBiP01134.
TreeFamiTF332938.

Family and domain databases

InterProiIPR000742. EG-like_dom.
IPR013032. EGF-like_CS.
IPR015497. EGF_rcpt_ligand.
[Graphical view]
PANTHERiPTHR10740. PTHR10740. 1 hit.
SMARTiSM00181. EGF. 1 hit.
[Graphical view]
PROSITEiPS00022. EGF_1. 1 hit.
PS01186. EGF_2. 1 hit.
PS50026. EGF_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P01134-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MVPAAGQLAL LALGILVAVC QALENSTSPL SDSPVAAAVV SHFNKCPDSH
60 70 80 90 100
TQYCFHGTCR FLVQEEKPAC VCHSGYVGVR CEHADLLAVV AASQKKQAIT
110 120 130 140 150
ALVVVSIVAL AVLIITCVLI HCCQVRKHCE WCRALVCRHE KPSALLKGRT

ACCHSETVV
Length:159
Mass (Da):16,960
Last modified:July 15, 1998 - v2
Checksum:iE9664EF04DFCF4D5
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti28 – 281S → P in CAA26036. (PubMed:3855503)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X02004 mRNA. Translation: CAA26036.1.
M31075 Genomic DNA. Translation: AAA42234.1.
M31076 mRNA. Translation: AAA42233.1.
PIRiA93356. WFRT1.
I57497.
RefSeqiNP_036803.1. NM_012671.2.
UniGeneiRn.9952.

Genome annotation databases

EnsembliENSRNOT00000057441; ENSRNOP00000054248; ENSRNOG00000016182.
GeneIDi24827.
KEGGirno:24827.
UCSCiRGD:3849. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X02004 mRNA. Translation: CAA26036.1.
M31075 Genomic DNA. Translation: AAA42234.1.
M31076 mRNA. Translation: AAA42233.1.
PIRiA93356. WFRT1.
I57497.
RefSeqiNP_036803.1. NM_012671.2.
UniGeneiRn.9952.

3D structure databases

ProteinModelPortaliP01134.
SMRiP01134. Positions 39-88.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000054248.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000057441; ENSRNOP00000054248; ENSRNOG00000016182.
GeneIDi24827.
KEGGirno:24827.
UCSCiRGD:3849. rat.

Organism-specific databases

CTDi7039.
RGDi3849. Tgfa.

Phylogenomic databases

eggNOGiNOG41326.
GeneTreeiENSGT00730000110951.
HOGENOMiHOG000013036.
HOVERGENiHBG000330.
InParanoidiP01134.
KOiK08774.
OMAiCHSETGC.
OrthoDBiEOG7VQJGP.
PhylomeDBiP01134.
TreeFamiTF332938.

Miscellaneous databases

NextBioi604544.
PROiP01134.

Gene expression databases

GenevestigatoriP01134.

Family and domain databases

InterProiIPR000742. EG-like_dom.
IPR013032. EGF-like_CS.
IPR015497. EGF_rcpt_ligand.
[Graphical view]
PANTHERiPTHR10740. PTHR10740. 1 hit.
SMARTiSM00181. EGF. 1 hit.
[Graphical view]
PROSITEiPS00022. EGF_1. 1 hit.
PS01186. EGF_2. 1 hit.
PS50026. EGF_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning and sequence analysis of a cDNA for rat transforming growth factor-alpha."
    Lee D.C., Rose T.M., Webb N.R., Todaro G.J.
    Nature 313:489-491(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Characterization of the rat transforming growth factor alpha gene and identification of promoter sequences."
    Blasband A.J., Rogers K.T., Chen X., Azizkhan J.C., Lee D.C.
    Mol. Cell. Biol. 10:2111-2121(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
  3. "Rat transforming growth factor type 1: structure and relation to epidermal growth factor."
    Marquardt H., Hunkapiller M.W., Hood L.E., Todaro G.J.
    Science 223:1079-1082(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 39-88.
  4. "Epidermal growth factor-like transforming growth factor. I. Isolation, chemical characterization, and potentiation by other transforming factors from feline sarcoma virus-transformed rat cells."
    Massague J.
    J. Biol. Chem. 258:13606-13613(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 39-67.

Entry informationi

Entry nameiTGFA_RAT
AccessioniPrimary (citable) accession number: P01134
Secondary accession number(s): Q63749
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 15, 1998
Last modified: January 7, 2015
This is version 125 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.