P01134 (TGFA_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protransforming growth factor alpha

Cleaved into the following chain:

1. Transforming growth factor alpha
   Short name=TGF-alpha
   Alternative name(s):
   EGF-like TGF
   Short name=ETGF
   TGF type 1

Gene names

Name:

Tgfa

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	159 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor/EGFR and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar.
Subunit structure	Interacts with the PDZ domains of MAGI3, SDCBP and SNTA1. The interaction with SDCBP, is required for the targeting to the cell surface. In the endoplasmic reticulum, in its immature form (i.e. with a prosegment and lacking full N-glycosylation), interacts with CNIH. In the Golgi apparatus, may form a complex with CNIH and GORASP2. Interacts (via cytoplasmic C-terminal domain) with NKD2 By similarity.
Subcellular location	Transforming growth factor alpha: Secreted › extracellular space. Protransforming growth factor alpha: Cell membrane; Single-pass type I membrane protein.
Sequence similarities	Contains 1 EGF-like domain.

Ontologies

Keywords
Cellular component	Cell membrane Membrane Secreted
Domain	EGF-like domain Signal Transmembrane Transmembrane helix
Molecular function	Growth factor Mitogen
PTM	Disulfide bond Glycoprotein Lipoprotein Palmitate
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	angiogenesis Inferred from electronic annotation. Source: Compara epidermal growth factor receptor signaling pathway Traceable author statement PubMed 2103501. Source: RGD mammary gland alveolus development Inferred from electronic annotation. Source: Compara negative regulation of apoptotic process Inferred from direct assay PubMed 11487584. Source: RGD positive regulation of cell division Inferred from electronic annotation. Source: UniProtKB-KW positive regulation of epidermal growth factor-activated receptor activity Inferred from sequence or structural similarity. Source: HGNC positive regulation of epithelial cell proliferation Inferred from sequence or structural similarity. Source: HGNC positive regulation of mitosis Inferred from sequence or structural similarity. Source: HGNC response to drug Inferred from expression pattern PubMed 16704299. Source: RGD wound healing Inferred from expression pattern PubMed 16966143. Source: RGD
Cellular_component	basolateral plasma membrane Inferred from electronic annotation. Source: Compara cell surface Inferred from electronic annotation. Source: Compara cytoplasmic vesicle Inferred from electronic annotation. Source: Compara extracellular space Inferred from direct assay PubMed 11487584. Source: RGD integral to plasma membrane Traceable author statement PubMed 2103501. Source: RGD nucleus Inferred from direct assay PubMed 14656002. Source: RGD perinuclear region of cytoplasm Inferred from electronic annotation. Source: Compara
Molecular_function	MAP kinase kinase activity Inferred from sequence or structural similarity. Source: HGNC epidermal growth factor receptor binding Inferred from sequence or structural similarity. Source: UniProtKB growth factor activity Inferred from sequence or structural similarity. Source: HGNC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Potential
	Chain	24 – 159	136	Protransforming growth factor alpha		PRO_0000302748
	Propeptide	24 – 38	15	Removed in mature form		PRO_0000007764
	Chain	39 – 88	50	Transforming growth factor alpha		PRO_0000007765
	Propeptide	89 – 159	71	Removed in mature form		PRO_0000007766
Regions
	Topological domain	24 – 97	74	Extracellular Potential
	Transmembrane	98 – 123	26	Helical; Potential
	Topological domain	124 – 159	36	Cytoplasmic Potential
	Domain	42 – 82	41	EGF-like
Amino acid modifications
	Lipidation	152	1	S-palmitoyl cysteine By similarity
	Lipidation	153	1	S-palmitoyl cysteine By similarity
	Glycosylation	25	1	N-linked (GlcNAc...) Potential
	Disulfide bond	46 ↔ 59		By similarity
	Disulfide bond	54 ↔ 70		By similarity
	Disulfide bond	72 ↔ 81		By similarity
Experimental info
	Sequence conflict	28	1	S → P in CAA26036. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P01134 [UniParc]. Last modified July 15, 1998. Version 2. Checksum: E9664EF04DFCF4D5	FASTA	159	16,960
	10 20 30 40 50 60 MVPAAGQLAL LALGILVAVC QALENSTSPL SDSPVAAAVV SHFNKCPDSH TQYCFHGTCR 70 80 90 100 110 120 FLVQEEKPAC VCHSGYVGVR CEHADLLAVV AASQKKQAIT ALVVVSIVAL AVLIITCVLI 130 140 150 HCCQVRKHCE WCRALVCRHE KPSALLKGRT ACCHSETVV

References

[1]	"Cloning and sequence analysis of a cDNA for rat transforming growth factor-alpha." Lee D.C., Rose T.M., Webb N.R., Todaro G.J. Nature 313:489-491(1985) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Characterization of the rat transforming growth factor alpha gene and identification of promoter sequences." Blasband A.J., Rogers K.T., Chen X., Azizkhan J.C., Lee D.C. Mol. Cell. Biol. 10:2111-2121(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
[3]	"Rat transforming growth factor type 1: structure and relation to epidermal growth factor." Marquardt H., Hunkapiller M.W., Hood L.E., Todaro G.J. Science 223:1079-1082(1984) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 39-88.
[4]	"Epidermal growth factor-like transforming growth factor. I. Isolation, chemical characterization, and potentiation by other transforming factors from feline sarcoma virus-transformed rat cells." Massague J. J. Biol. Chem. 258:13606-13613(1983) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 39-67.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X02004 mRNA. Translation: CAA26036.1. M31075 Genomic DNA. Translation: AAA42234.1. M31076 mRNA. Translation: AAA42233.1.
IPI	IPI00779017.
PIR	WFRT1. A93356. I57497.
RefSeq	NP_036803.1. NM_012671.2.
UniGene	Rn.9952.
3D structure databases
ProteinModelPortal	P01134.
SMR	P01134. Positions 39-88.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000054248.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000057441; ENSRNOP00000054248; ENSRNOG00000016182.
GeneID	24827.
KEGG	rno:24827.
UCSC	RGD:3849. rat.
Organism-specific databases
CTD	7039.
RGD	3849. Tgfa.
Phylogenomic databases
eggNOG	NOG41326.
GeneTree	ENSGT00530000062996.
HOGENOM	HOG000013036.
HOVERGEN	HBG000330.
InParanoid	P01134.
KO	K08774.
OrthoDB	EOG4SN1PX.
Gene expression databases
ArrayExpress	P01134.
Genevestigator	P01134.
Family and domain databases
InterPro	IPR000742. EG-like_dom. IPR013032. EGF-like_CS. IPR015497. EGF_rcpt_ligand. [Graphical view]
PANTHER	PTHR10740. PTHR10740. 1 hit.
SMART	SM00181. EGF. 1 hit. [Graphical view]
PROSITE	PS00022. EGF_1. 1 hit. PS01186. EGF_2. 1 hit. PS50026. EGF_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	604544.

Entry information

Entry name

TGFA_RAT

Accession

Primary (citable) accession number: P01134
Secondary accession number(s): Q63749

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 15, 1998
Last modified:	April 3, 2013
This is version 117 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program