Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Protransforming growth factor alpha

Gene

Tgfa

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor/EGFR and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar.

GO - Molecular functioni

GO - Biological processi

  • activation of MAPK activity Source: HGNC
  • angiogenesis Source: Ensembl
  • epidermal growth factor receptor signaling pathway Source: RGD
  • hepatocyte proliferation Source: UniProtKB
  • mammary gland alveolus development Source: Ensembl
  • negative regulation of apoptotic process Source: RGD
  • negative regulation of cellular process Source: RGD
  • positive regulation of cell division Source: UniProtKB-KW
  • positive regulation of cell proliferation Source: RGD
  • positive regulation of epidermal growth factor-activated receptor activity Source: HGNC
  • positive regulation of epithelial cell proliferation Source: HGNC
  • positive regulation of mitotic nuclear division Source: HGNC
  • response to drug Source: RGD
  • wound healing Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Growth factor, Mitogen

Enzyme and pathway databases

ReactomeiR-RNO-204005. COPII (Coat Protein 2) Mediated Vesicle Transport.
R-RNO-5694530. Cargo concentration in the ER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protransforming growth factor alpha
Cleaved into the following chain:
Alternative name(s):
EGF-like TGF
Short name:
ETGF
TGF type 1
Gene namesi
Name:Tgfa
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 4

Organism-specific databases

RGDi3849. Tgfa.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini24 – 97ExtracellularSequence analysisAdd BLAST74
Transmembranei98 – 123HelicalSequence analysisAdd BLAST26
Topological domaini124 – 159CytoplasmicSequence analysisAdd BLAST36

GO - Cellular componenti

  • extracellular space Source: RGD
  • integral component of plasma membrane Source: RGD
  • nucleus Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 23Sequence analysisAdd BLAST23
ChainiPRO_000030274824 – 159Protransforming growth factor alphaAdd BLAST136
PropeptideiPRO_000000776424 – 38Removed in mature formAdd BLAST15
ChainiPRO_000000776539 – 88Transforming growth factor alphaAdd BLAST50
PropeptideiPRO_000000776689 – 159Removed in mature formAdd BLAST71

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi25N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi46 ↔ 59PROSITE-ProRule annotation
Disulfide bondi54 ↔ 70PROSITE-ProRule annotation
Disulfide bondi72 ↔ 81PROSITE-ProRule annotation
Lipidationi152S-palmitoyl cysteineBy similarity1
Lipidationi153S-palmitoyl cysteineBy similarity1

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate

Proteomic databases

PaxDbiP01134.
PRIDEiP01134.

Expressioni

Gene expression databases

BgeeiENSRNOG00000016182.
GenevisibleiP01134. RN.

Interactioni

Subunit structurei

Interacts with the PDZ domains of MAGI3, SDCBP and SNTA1. The interaction with SDCBP, is required for the targeting to the cell surface. In the endoplasmic reticulum, in its immature form (i.e. with a prosegment and lacking full N-glycosylation), interacts with CNIH. In the Golgi apparatus, may form a complex with CNIH and GORASP2. Interacts (via cytoplasmic C-terminal domain) with NKD2 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000054248.

Structurei

3D structure databases

ProteinModelPortaliP01134.
SMRiP01134.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini42 – 82EGF-likePROSITE-ProRule annotationAdd BLAST41

Sequence similaritiesi

Contains 1 EGF-like domain.PROSITE-ProRule annotation

Keywords - Domaini

EGF-like domain, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IVW5. Eukaryota.
ENOG4111Z4I. LUCA.
GeneTreeiENSGT00730000110951.
HOGENOMiHOG000013036.
HOVERGENiHBG000330.
InParanoidiP01134.
KOiK08774.
OMAiLIHCCEV.
OrthoDBiEOG091G0RK4.
PhylomeDBiP01134.
TreeFamiTF332938.

Family and domain databases

InterProiIPR013032. EGF-like_CS.
IPR000742. EGF-like_dom.
IPR015497. EGF_rcpt_ligand.
[Graphical view]
PANTHERiPTHR10740. PTHR10740. 1 hit.
PROSITEiPS00022. EGF_1. 1 hit.
PS01186. EGF_2. 1 hit.
PS50026. EGF_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P01134-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVPAAGQLAL LALGILVAVC QALENSTSPL SDSPVAAAVV SHFNKCPDSH
60 70 80 90 100
TQYCFHGTCR FLVQEEKPAC VCHSGYVGVR CEHADLLAVV AASQKKQAIT
110 120 130 140 150
ALVVVSIVAL AVLIITCVLI HCCQVRKHCE WCRALVCRHE KPSALLKGRT

ACCHSETVV
Length:159
Mass (Da):16,960
Last modified:July 15, 1998 - v2
Checksum:iE9664EF04DFCF4D5
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti28S → P in CAA26036 (PubMed:3855503).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X02004 mRNA. Translation: CAA26036.1.
M31075 Genomic DNA. Translation: AAA42234.1.
M31076 mRNA. Translation: AAA42233.1.
PIRiA93356. WFRT1.
I57497.
RefSeqiNP_036803.1. NM_012671.2.
UniGeneiRn.9952.

Genome annotation databases

EnsembliENSRNOT00000057441; ENSRNOP00000054248; ENSRNOG00000016182.
GeneIDi24827.
KEGGirno:24827.
UCSCiRGD:3849. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X02004 mRNA. Translation: CAA26036.1.
M31075 Genomic DNA. Translation: AAA42234.1.
M31076 mRNA. Translation: AAA42233.1.
PIRiA93356. WFRT1.
I57497.
RefSeqiNP_036803.1. NM_012671.2.
UniGeneiRn.9952.

3D structure databases

ProteinModelPortaliP01134.
SMRiP01134.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000054248.

Proteomic databases

PaxDbiP01134.
PRIDEiP01134.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000057441; ENSRNOP00000054248; ENSRNOG00000016182.
GeneIDi24827.
KEGGirno:24827.
UCSCiRGD:3849. rat.

Organism-specific databases

CTDi7039.
RGDi3849. Tgfa.

Phylogenomic databases

eggNOGiENOG410IVW5. Eukaryota.
ENOG4111Z4I. LUCA.
GeneTreeiENSGT00730000110951.
HOGENOMiHOG000013036.
HOVERGENiHBG000330.
InParanoidiP01134.
KOiK08774.
OMAiLIHCCEV.
OrthoDBiEOG091G0RK4.
PhylomeDBiP01134.
TreeFamiTF332938.

Enzyme and pathway databases

ReactomeiR-RNO-204005. COPII (Coat Protein 2) Mediated Vesicle Transport.
R-RNO-5694530. Cargo concentration in the ER.

Miscellaneous databases

PROiP01134.

Gene expression databases

BgeeiENSRNOG00000016182.
GenevisibleiP01134. RN.

Family and domain databases

InterProiIPR013032. EGF-like_CS.
IPR000742. EGF-like_dom.
IPR015497. EGF_rcpt_ligand.
[Graphical view]
PANTHERiPTHR10740. PTHR10740. 1 hit.
PROSITEiPS00022. EGF_1. 1 hit.
PS01186. EGF_2. 1 hit.
PS50026. EGF_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTGFA_RAT
AccessioniPrimary (citable) accession number: P01134
Secondary accession number(s): Q63749
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 15, 1998
Last modified: November 2, 2016
This is version 138 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.