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P01134

- TGFA_RAT

UniProt

P01134 - TGFA_RAT

Protein

Protransforming growth factor alpha

Gene

Tgfa

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor/EGFR and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar.

    GO - Molecular functioni

    1. epidermal growth factor receptor binding Source: UniProtKB
    2. growth factor activity Source: HGNC
    3. MAP kinase kinase activity Source: HGNC

    GO - Biological processi

    1. activation of MAPK activity Source: HGNC
    2. angiogenesis Source: Ensembl
    3. epidermal growth factor receptor signaling pathway Source: RGD
    4. mammary gland alveolus development Source: Ensembl
    5. negative regulation of apoptotic process Source: RGD
    6. negative regulation of cellular process Source: RGD
    7. positive regulation of cell division Source: UniProtKB-KW
    8. positive regulation of cell proliferation Source: RGD
    9. positive regulation of epidermal growth factor-activated receptor activity Source: HGNC
    10. positive regulation of epithelial cell proliferation Source: HGNC
    11. positive regulation of mitosis Source: HGNC
    12. response to drug Source: RGD
    13. wound healing Source: RGD

    Keywords - Molecular functioni

    Growth factor, Mitogen

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protransforming growth factor alpha
    Cleaved into the following chain:
    Alternative name(s):
    EGF-like TGF
    Short name:
    ETGF
    TGF type 1
    Gene namesi
    Name:Tgfa
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 4

    Organism-specific databases

    RGDi3849. Tgfa.

    Subcellular locationi

    GO - Cellular componenti

    1. basolateral plasma membrane Source: Ensembl
    2. cell surface Source: Ensembl
    3. cytoplasmic vesicle Source: Ensembl
    4. extracellular space Source: RGD
    5. integral component of plasma membrane Source: RGD
    6. nucleus Source: RGD
    7. perinuclear region of cytoplasm Source: Ensembl

    Keywords - Cellular componenti

    Cell membrane, Membrane, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2323Sequence AnalysisAdd
    BLAST
    Chaini24 – 159136Protransforming growth factor alphaPRO_0000302748Add
    BLAST
    Propeptidei24 – 3815Removed in mature formPRO_0000007764Add
    BLAST
    Chaini39 – 8850Transforming growth factor alphaPRO_0000007765Add
    BLAST
    Propeptidei89 – 15971Removed in mature formPRO_0000007766Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi25 – 251N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi46 ↔ 59PROSITE-ProRule annotation
    Disulfide bondi54 ↔ 70PROSITE-ProRule annotation
    Disulfide bondi72 ↔ 81PROSITE-ProRule annotation
    Lipidationi152 – 1521S-palmitoyl cysteineBy similarity
    Lipidationi153 – 1531S-palmitoyl cysteineBy similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Lipoprotein, Palmitate

    Expressioni

    Gene expression databases

    GenevestigatoriP01134.

    Interactioni

    Subunit structurei

    Interacts with the PDZ domains of MAGI3, SDCBP and SNTA1. The interaction with SDCBP, is required for the targeting to the cell surface. In the endoplasmic reticulum, in its immature form (i.e. with a prosegment and lacking full N-glycosylation), interacts with CNIH. In the Golgi apparatus, may form a complex with CNIH and GORASP2. Interacts (via cytoplasmic C-terminal domain) with NKD2 By similarity.By similarity

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000054248.

    Structurei

    3D structure databases

    ProteinModelPortaliP01134.
    SMRiP01134. Positions 39-88.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini24 – 9774ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini124 – 15936CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei98 – 12326HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini42 – 8241EGF-likePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 EGF-like domain.PROSITE-ProRule annotation

    Keywords - Domaini

    EGF-like domain, Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG41326.
    GeneTreeiENSGT00730000110951.
    HOGENOMiHOG000013036.
    HOVERGENiHBG000330.
    InParanoidiP01134.
    KOiK08774.
    OMAiCHSETGC.
    OrthoDBiEOG7VQJGP.
    PhylomeDBiP01134.
    TreeFamiTF332938.

    Family and domain databases

    InterProiIPR000742. EG-like_dom.
    IPR013032. EGF-like_CS.
    IPR015497. EGF_rcpt_ligand.
    [Graphical view]
    PANTHERiPTHR10740. PTHR10740. 1 hit.
    SMARTiSM00181. EGF. 1 hit.
    [Graphical view]
    PROSITEiPS00022. EGF_1. 1 hit.
    PS01186. EGF_2. 1 hit.
    PS50026. EGF_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P01134-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVPAAGQLAL LALGILVAVC QALENSTSPL SDSPVAAAVV SHFNKCPDSH    50
    TQYCFHGTCR FLVQEEKPAC VCHSGYVGVR CEHADLLAVV AASQKKQAIT 100
    ALVVVSIVAL AVLIITCVLI HCCQVRKHCE WCRALVCRHE KPSALLKGRT 150
    ACCHSETVV 159
    Length:159
    Mass (Da):16,960
    Last modified:July 15, 1998 - v2
    Checksum:iE9664EF04DFCF4D5
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti28 – 281S → P in CAA26036. (PubMed:3855503)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X02004 mRNA. Translation: CAA26036.1.
    M31075 Genomic DNA. Translation: AAA42234.1.
    M31076 mRNA. Translation: AAA42233.1.
    PIRiA93356. WFRT1.
    I57497.
    RefSeqiNP_036803.1. NM_012671.2.
    UniGeneiRn.9952.

    Genome annotation databases

    EnsembliENSRNOT00000057441; ENSRNOP00000054248; ENSRNOG00000016182.
    GeneIDi24827.
    KEGGirno:24827.
    UCSCiRGD:3849. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X02004 mRNA. Translation: CAA26036.1 .
    M31075 Genomic DNA. Translation: AAA42234.1 .
    M31076 mRNA. Translation: AAA42233.1 .
    PIRi A93356. WFRT1.
    I57497.
    RefSeqi NP_036803.1. NM_012671.2.
    UniGenei Rn.9952.

    3D structure databases

    ProteinModelPortali P01134.
    SMRi P01134. Positions 39-88.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000054248.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000057441 ; ENSRNOP00000054248 ; ENSRNOG00000016182 .
    GeneIDi 24827.
    KEGGi rno:24827.
    UCSCi RGD:3849. rat.

    Organism-specific databases

    CTDi 7039.
    RGDi 3849. Tgfa.

    Phylogenomic databases

    eggNOGi NOG41326.
    GeneTreei ENSGT00730000110951.
    HOGENOMi HOG000013036.
    HOVERGENi HBG000330.
    InParanoidi P01134.
    KOi K08774.
    OMAi CHSETGC.
    OrthoDBi EOG7VQJGP.
    PhylomeDBi P01134.
    TreeFami TF332938.

    Miscellaneous databases

    NextBioi 604544.
    PROi P01134.

    Gene expression databases

    Genevestigatori P01134.

    Family and domain databases

    InterProi IPR000742. EG-like_dom.
    IPR013032. EGF-like_CS.
    IPR015497. EGF_rcpt_ligand.
    [Graphical view ]
    PANTHERi PTHR10740. PTHR10740. 1 hit.
    SMARTi SM00181. EGF. 1 hit.
    [Graphical view ]
    PROSITEi PS00022. EGF_1. 1 hit.
    PS01186. EGF_2. 1 hit.
    PS50026. EGF_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and sequence analysis of a cDNA for rat transforming growth factor-alpha."
      Lee D.C., Rose T.M., Webb N.R., Todaro G.J.
      Nature 313:489-491(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Characterization of the rat transforming growth factor alpha gene and identification of promoter sequences."
      Blasband A.J., Rogers K.T., Chen X., Azizkhan J.C., Lee D.C.
      Mol. Cell. Biol. 10:2111-2121(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
    3. "Rat transforming growth factor type 1: structure and relation to epidermal growth factor."
      Marquardt H., Hunkapiller M.W., Hood L.E., Todaro G.J.
      Science 223:1079-1082(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 39-88.
    4. "Epidermal growth factor-like transforming growth factor. I. Isolation, chemical characterization, and potentiation by other transforming factors from feline sarcoma virus-transformed rat cells."
      Massague J.
      J. Biol. Chem. 258:13606-13613(1983) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 39-67.

    Entry informationi

    Entry nameiTGFA_RAT
    AccessioniPrimary (citable) accession number: P01134
    Secondary accession number(s): Q63749
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: July 15, 1998
    Last modified: October 1, 2014
    This is version 124 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3