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Protein

Protransforming growth factor alpha

Gene

Tgfa

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor/EGFR and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar.

GO - Molecular functioni

  • epidermal growth factor receptor binding Source: RGD
  • growth factor activity Source: HGNC

GO - Biological processi

  • activation of MAPK activity Source: HGNC
  • angiogenesis Source: Ensembl
  • epidermal growth factor receptor signaling pathway Source: RGD
  • hepatocyte proliferation Source: UniProtKB
  • mammary gland alveolus development Source: Ensembl
  • negative regulation of apoptotic process Source: RGD
  • negative regulation of cellular process Source: RGD
  • positive regulation of cell division Source: UniProtKB-KW
  • positive regulation of cell proliferation Source: RGD
  • positive regulation of epidermal growth factor-activated receptor activity Source: HGNC
  • positive regulation of epithelial cell proliferation Source: HGNC
  • positive regulation of mitotic nuclear division Source: HGNC
  • response to drug Source: RGD
  • wound healing Source: RGD

Keywordsi

Molecular functionGrowth factor, Mitogen

Enzyme and pathway databases

ReactomeiR-RNO-204005 COPII-mediated vesicle transport
R-RNO-5694530 Cargo concentration in the ER

Names & Taxonomyi

Protein namesi
Recommended name:
Protransforming growth factor alpha
Cleaved into the following chain:
Alternative name(s):
EGF-like TGF
Short name:
ETGF
TGF type 1
Gene namesi
Name:Tgfa
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 4

Organism-specific databases

RGDi3849 Tgfa

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini24 – 97ExtracellularSequence analysisAdd BLAST74
Transmembranei98 – 123HelicalSequence analysisAdd BLAST26
Topological domaini124 – 159CytoplasmicSequence analysisAdd BLAST36

Keywords - Cellular componenti

Cell membrane, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 23Sequence analysisAdd BLAST23
ChainiPRO_000030274824 – 159Protransforming growth factor alphaAdd BLAST136
PropeptideiPRO_000000776424 – 38Removed in mature formAdd BLAST15
ChainiPRO_000000776539 – 88Transforming growth factor alphaAdd BLAST50
PropeptideiPRO_000000776689 – 159Removed in mature formAdd BLAST71

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi25N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi46 ↔ 59PROSITE-ProRule annotation
Disulfide bondi54 ↔ 70PROSITE-ProRule annotation
Disulfide bondi72 ↔ 81PROSITE-ProRule annotation
Lipidationi152S-palmitoyl cysteineBy similarity1
Lipidationi153S-palmitoyl cysteineBy similarity1

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate

Proteomic databases

PaxDbiP01134
PRIDEiP01134

Expressioni

Gene expression databases

BgeeiENSRNOG00000016182
GenevisibleiP01134 RN

Interactioni

Subunit structurei

Interacts with the PDZ domains of MAGI3, SDCBP and SNTA1. The interaction with SDCBP, is required for the targeting to the cell surface. In the endoplasmic reticulum, in its immature form (i.e. with a prosegment and lacking full N-glycosylation), interacts with CNIH. In the Golgi apparatus, may form a complex with CNIH and GORASP2. Interacts (via cytoplasmic C-terminal domain) with NKD2 (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
SDCBP2Q9H1906EBI-16418721,EBI-742426From Homo sapiens.

GO - Molecular functioni

  • epidermal growth factor receptor binding Source: RGD
  • growth factor activity Source: HGNC

Protein-protein interaction databases

IntActiP01134, 2 interactors
STRINGi10116.ENSRNOP00000054248

Structurei

3D structure databases

ProteinModelPortaliP01134
SMRiP01134
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini42 – 82EGF-likePROSITE-ProRule annotationAdd BLAST41

Keywords - Domaini

EGF-like domain, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IVW5 Eukaryota
ENOG4111Z4I LUCA
GeneTreeiENSGT00730000110951
HOGENOMiHOG000013036
HOVERGENiHBG000330
InParanoidiP01134
KOiK08774
OMAiLIHCCEV
OrthoDBiEOG091G0RK4
PhylomeDBiP01134
TreeFamiTF332938

Family and domain databases

InterProiView protein in InterPro
IPR013032 EGF-like_CS
IPR000742 EGF-like_dom
IPR015497 EGF_rcpt_ligand
PANTHERiPTHR10740 PTHR10740, 1 hit
PROSITEiView protein in PROSITE
PS00022 EGF_1, 1 hit
PS01186 EGF_2, 1 hit
PS50026 EGF_3, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P01134-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVPAAGQLAL LALGILVAVC QALENSTSPL SDSPVAAAVV SHFNKCPDSH
60 70 80 90 100
TQYCFHGTCR FLVQEEKPAC VCHSGYVGVR CEHADLLAVV AASQKKQAIT
110 120 130 140 150
ALVVVSIVAL AVLIITCVLI HCCQVRKHCE WCRALVCRHE KPSALLKGRT

ACCHSETVV
Length:159
Mass (Da):16,960
Last modified:July 15, 1998 - v2
Checksum:iE9664EF04DFCF4D5
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti28S → P in CAA26036 (PubMed:3855503).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X02004 mRNA Translation: CAA26036.1
M31075 Genomic DNA Translation: AAA42234.1
M31076 mRNA Translation: AAA42233.1
PIRiA93356 WFRT1
I57497
RefSeqiNP_036803.1, NM_012671.2
UniGeneiRn.9952

Genome annotation databases

EnsembliENSRNOT00000057441; ENSRNOP00000054248; ENSRNOG00000016182
GeneIDi24827
KEGGirno:24827
UCSCiRGD:3849 rat

Similar proteinsi

Entry informationi

Entry nameiTGFA_RAT
AccessioniPrimary (citable) accession number: P01134
Secondary accession number(s): Q63749
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 15, 1998
Last modified: May 23, 2018
This is version 145 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

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