P01129 (CDC10_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Start control protein cdc10

Gene names

Name:	cdc10
ORF Names:	SPBC336.12c

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) [Reference proteome]

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces › Schizosaccharomyces pombe

Protein attributes

Sequence length	767 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Major component of the cell cycle transcription factor complex MBF (MCB binding factor, also known as DSC1), that controls G1-S phase specific gene expression. Involved in the control of rRNA production, via interaction with pol5. May be involved in the transcriptional regulation of the cdc22 and cdt1 genes. In fission yeast, two genes, cdc10 and cdc2, are required for the cell cycle control called start, the point early in the G1 phase at which cells become committed to the mitotic cycle. Ref.4 Ref.5
Subunit structure	DSC1 contains cdc10 and sct1/res1. Interacts with pol5. Ref.5
Subcellular location	Nucleus Ref.3.
Sequence similarities	Contains 2 ANK repeats. Contains 1 HTH APSES-type DNA-binding domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division Mitosis
Cellular component	Nucleus
Domain	ANK repeat Repeat
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell division Inferred from electronic annotation. Source: UniProtKB-KW mitosis Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of transcription during meiosis Inferred from direct assay Ref.4. Source: UniProtKB negative regulation of transcription during mitosis Inferred from direct assay Ref.4. Source: UniProtKB positive regulation of transcription during meiosis Inferred from direct assay Ref.4. Source: UniProtKB positive regulation of transcription during mitosis Inferred from direct assay Ref.4. Source: UniProtKB positive regulation of transcription involved in G1/S phase of mitotic cell cycle Inferred from mutant phenotype PubMed 19714215. Source: PomBase rRNA transcription Inferred from direct assay Ref.5. Source: UniProtKB regulation of S phase of mitotic cell cycle Inferred from genetic interaction PubMed 10982385. Source: PomBase
Cellular_component	MBF transcription complex Traceable author statement PubMed 7739540. Source: PomBase cytoplasm Inferred from direct assay PubMed 16823372. Source: PomBase
Molecular_function	RNA polymerase II core promoter proximal region sequence-specific DNA binding Inferred by curator. Source: PomBase sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
pol5	O60094	2	EBI-1009350,EBI-1009362

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 767	767	Start control protein cdc10		PRO_0000067060
Regions
	Domain	66 – 173	108	HTH APSES-type
	Repeat	356 – 385	30	ANK 1
	Repeat	483 – 512	30	ANK 2
	DNA binding	98 – 119	22	H-T-H motif By similarity
	Motif	261 – 264	4	Nuclear localization signal Potential
Amino acid modifications
	Modified residue	252	1	Phosphoserine Ref.6

Sequences

	Sequence		Length	Mass (Da)
	P01129 [UniParc]. Last modified July 21, 1986. Version 1. Checksum: 0E514EE1BE218EFA	FASTA	767	85,513
	10 20 30 40 50 60 MASANFIRQF ELGNDSFSYQ KRPEDEPSQP LSNRNINKLN DSSTLKDSSS RIFINSQVLR 70 80 90 100 110 120 DGRPVELYAV ECSGMKYMEL SCGDNVALRR CPDSYFNISQ ILRLAGTSSS ENAKELDDII 130 140 150 160 170 180 ESGDYENVDS KHPQIDGVWV PYDRAISIAK RYGVYEILQP LISFNLDLFP KFSKQQQIES 190 200 210 220 230 240 SSISKNLNTS SFNTRSPLRN HNFSNPSKSS KNGVHTINNM QSSPSPSSSF LLPLTQIDSQ 250 260 270 280 290 300 NVKRSNNYLS TSPPILEQRL KRHRIDVSDE DLHPSSQLND NEASSLFPDT PRLNHSLSFV 310 320 330 340 350 360 SLVSSLPPLD QNIMQDYHTS KDILTSIFLD VNFADSSALE AKLSDSLDLD VPIDELGHAA 370 380 390 400 410 420 LHWAAAVAKM PLLQALIHKG ANPLRGNLTG ETALMRSVLV TNHLNQNSFG DLLDLLYASL 430 440 450 460 470 480 PCTDRAGRTV VHHICLTAGI KGRGSASRYY LETLLNWAKK HASGNNGYML KDFINYLNHQ 490 500 510 520 530 540 DKNGDTALNI AARIGNKNIV EVLMQAGASA YIPNRAGLSV ANFGIFVENA LKQPEDSKQT 550 560 570 580 590 600 KVSLMSENLS SKEKTAVPPR QKSRDIIASV TDVISSLDKD FQDEMAAKQS MIDSAYTQLR 610 620 630 640 650 660 ESTKKLSDLR EQLHVSETQR TLFLELRQRC KNLMTSIEEQ KSELSNLYES FDPNGIHDSL 670 680 690 700 710 720 SLDADAPFTV NENNNKNLSI AELKFQVAAY ERNEARLNEL ANKLWQRNSN IKSKCRRVVS 730 740 750 760 LCTGVDESRV DSLLESLLQA VESDGQQGEV DMGRVAGFLR VVKEHQA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning, sequencing and transcriptional control of the Schizosaccharomyces pombe cdc10 'start' gene." Aves S.J., Durkacz B.W., Carr A., Nurse P. EMBO J. 4:457-463(1985) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.
[3]	"Large-scale screening of intracellular protein localization in living fission yeast cells by the use of a GFP-fusion genomic DNA library." Ding D.-Q., Tomita Y., Yamamoto A., Chikashige Y., Haraguchi T., Hiraoka Y. Genes Cells 5:169-190(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 76-310, SUBCELLULAR LOCATION. Strain: ATCC 38364 / 968.
[4]	"Positive and negative roles for cdc10 in cell cycle gene expression." McInerny C.J., Kersey P.J., Creanor J., Fantes P.A. Nucleic Acids Res. 23:4761-4768(1995) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[5]	"Pol5p, a novel binding partner to Cdc10p in fission yeast involved in rRNA production." Nadeem F.K., Blair D., McInerny C.J. Mol. Genet. Genomics 276:391-401(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH POL5.
[6]	"Phosphoproteome analysis of fission yeast." Wilson-Grady J.T., Villen J., Gygi S.P. J. Proteome Res. 7:1088-1097(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-252, MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X02175 Genomic DNA. Translation: CAA26116.1. CU329671 Genomic DNA. Translation: CAB58164.1. AB027889 Genomic DNA. Translation: BAA87193.1.
PIR	COZPCD. A01382.
RefSeq	NP_596132.1. NM_001022050.2.
3D structure databases
ProteinModelPortal	P01129.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-353N.
IntAct	P01129. 2 interactions.
MINT	MINT-4686691.
STRING	4896.SPBC336.12c-1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SPBC336.12c.1; SPBC336.12c.1:pep; SPBC336.12c.
GeneID	2540234.
KEGG	spo:SPBC336.12c.
Organism-specific databases
PomBase	SPBC336.12c.
Phylogenomic databases
eggNOG	COG0666.
KO	K06867.
OrthoDB	EOG40KC7F.
Family and domain databases
Gene3D	1.25.40.20. 1 hit. 3.10.260.10. 1 hit.
InterPro	IPR002110. Ankyrin_rpt. IPR020683. Ankyrin_rpt-contain_dom. IPR003163. Tscrpt_reg_HTH_APSES-type. [Graphical view]
Pfam	PF00023. Ank. 1 hit. [Graphical view]
SMART	SM00248. ANK. 2 hits. [Graphical view]
SUPFAM	SSF48403. ANK. 1 hit.
PROSITE	PS50297. ANK_REP_REGION. 1 hit. PS50088. ANK_REPEAT. 2 hits. PS51299. HTH_APSES. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20801365.

Entry information

Entry name

CDC10_SCHPO

Accession

Primary (citable) accession number: P01129
Secondary accession number(s): Q9UU04

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	May 29, 2013
This is version 120 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program