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Protein

Ras-like protein 1

Gene

RAS1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The S.cerevisiae Ras proteins modulate the activity of the adenylate cyclase catalytic subunit and therefore affect the biosynthesis of cyclic-AMP.

Miscellaneous

Present with 2050 molecules/cell in log phase SD medium.1 Publication

Enzyme regulationi

Alternates between an inactive form bound to GDP and an active form bound to GTP. Activated by guanine nucleotide-exchange factor (GEF) CDC25 and inactivated by GTPase-activating proteins (GAPs) IRA1 and IRA2.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi17 – 24GTPBy similarity8
Nucleotide bindingi64 – 68GTPBy similarity5
Nucleotide bindingi123 – 126GTPBy similarity4

GO - Molecular functioni

  • GTPase activity Source: SGD
  • GTP binding Source: UniProtKB-KW

GO - Biological processi

  • adenylate cyclase-activating G-protein coupled receptor signaling pathway Source: SGD
  • positive regulation of adenylate cyclase activity Source: SGD
  • protein localization to bud neck Source: SGD
  • replicative cell aging Source: SGD

Keywordsi

LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-33634-MONOMER
ReactomeiR-SCE-1445148 Translocation of GLUT4 to the plasma membrane
R-SCE-171007 p38MAPK events
R-SCE-5218921 VEGFR2 mediated cell proliferation
R-SCE-5658442 Regulation of RAS by GAPs
R-SCE-5673001 RAF/MAP kinase cascade
R-SCE-5675221 Negative regulation of MAPK pathway
R-SCE-6798695 Neutrophil degranulation
R-SCE-8849471 PTK6 Regulates RHO GTPases, RAS GTPase and MAP kinases

Names & Taxonomyi

Protein namesi
Recommended name:
Ras-like protein 1
Gene namesi
Name:RAS1
Ordered Locus Names:YOR101W
ORF Names:YOR3205W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR101W
SGDiS000005627 RAS1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi66A → T: Impaired GTPase activity, autophosphorylating activity. 1
Mutagenesisi68Q → L: Impaired GTPase activity. 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000301931 – 306Ras-like protein 1Add BLAST306
PropeptideiPRO_0000030194307 – 309Removed in mature form3

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi305S-palmitoyl cysteine1 Publication1
Modified residuei306Cysteine methyl ester1 Publication1
Lipidationi306S-farnesyl cysteine1 Publication1

Post-translational modificationi

Farnesylated by RAM1-RAM2, which is required for targeting RAS1 to the cytoplasmic site of the endoplasmic reticulum, where proteolytic processing of the C-terminus by RCE1 and methylation of the resulting carboxyl group by STE14 occurs.2 Publications
Palmitoylated by the ERF2-SHR5 complex, which is required for proper plasma membrane localization of RAS1.By similarity

Keywords - PTMi

Lipoprotein, Methylation, Palmitate, Prenylation

Proteomic databases

MaxQBiP01119
PaxDbiP01119
PRIDEiP01119

PTM databases

iPTMnetiP01119
SwissPalmiP01119

Interactioni

Protein-protein interaction databases

BioGridi34499, 77 interactors
DIPiDIP-1041N
IntActiP01119, 8 interactors
MINTiP01119
STRINGi4932.YOR101W

Structurei

3D structure databases

ProteinModelPortaliP01119
SMRiP01119
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi39 – 47Effector region9

Sequence similaritiesi

Belongs to the small GTPase superfamily. Ras family.Curated

Phylogenomic databases

GeneTreeiENSGT00860000133672
HOGENOMiHOG000233973
InParanoidiP01119
KOiK07827
OMAiANNDHYL
OrthoDBiEOG092C4VY0

Family and domain databases

InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR005225 Small_GTP-bd_dom
IPR001806 Small_GTPase
IPR020849 Small_GTPase_Ras-type
PANTHERiPTHR24070 PTHR24070, 1 hit
PfamiView protein in Pfam
PF00071 Ras, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS51421 RAS, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P01119-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQGNKSTIRE YKIVVVGGGG VGKSALTIQF IQSYFVDEYD PTIEDSYRKQ
60 70 80 90 100
VVIDDKVSIL DILDTAGQEE YSAMREQYMR TGEGFLLVYS VTSRNSFDEL
110 120 130 140 150
LSYYQQIQRV KDSDYIPVVV VGNKLDLENE RQVSYEDGLR LAKQLNAPFL
160 170 180 190 200
ETSAKQAINV DEAFYSLIRL VRDDGGKYNS MNRQLDNTNE IRDSELTSSA
210 220 230 240 250
TADREKKNNG SYVLDNSLTN AGTGSSSKSA VNHNGETTKR TDEKNYVNQN
260 270 280 290 300
NNNEGNTKYS SNGNGNRSDI SRGNQNNALN SRSKQSAEPQ KNSSANARKE

SSGGCCIIC
Length:309
Mass (Da):34,347
Last modified:October 1, 1996 - v2
Checksum:iA70D720EB67E2F91
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti204R → I in AAA34958 (PubMed:6365329).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K01970 Genomic DNA Translation: AAA34958.1
X00527 Genomic DNA Translation: CAA25206.1
X94335 Genomic DNA Translation: CAA64023.1
Z75009 Genomic DNA Translation: CAA99298.1
U32307 Genomic DNA Translation: AAC49087.1
BK006948 Genomic DNA Translation: DAA10878.1
PIRiS61661 TVBYR1
RefSeqiNP_014744.1, NM_001183520.1

Genome annotation databases

EnsemblFungiiYOR101W; YOR101W; YOR101W
GeneIDi854268
KEGGisce:YOR101W

Similar proteinsi

Entry informationi

Entry nameiRAS1_YEAST
AccessioniPrimary (citable) accession number: P01119
Secondary accession number(s): D6W2G2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: October 1, 1996
Last modified: May 23, 2018
This is version 176 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

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