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Protein

Ras-like protein 1

Gene

RAS1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The S.cerevisiae Ras proteins modulate the activity of the adenylate cyclase catalytic subunit and therefore affect the biosynthesis of cyclic-AMP.

Enzyme regulationi

Alternates between an inactive form bound to GDP and an active form bound to GTP. Activated by guanine nucleotide-exchange factor (GEF) CDC25 and inactivated by GTPase-activating proteins (GAPs) IRA1 and IRA2.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi17 – 248GTPBy similarity
Nucleotide bindingi64 – 685GTPBy similarity
Nucleotide bindingi123 – 1264GTPBy similarity

GO - Molecular functioni

  • GTPase activity Source: SGD
  • GTP binding Source: UniProtKB-KW

GO - Biological processi

  • adenylate cyclase-activating G-protein coupled receptor signaling pathway Source: SGD
  • positive regulation of adenylate cyclase activity Source: SGD
  • protein localization to bud neck Source: SGD
  • replicative cell aging Source: SGD
  • small GTPase mediated signal transduction Source: InterPro
Complete GO annotation...

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-33634-MONOMER.
ReactomeiR-SCE-1445148. Translocation of GLUT4 to the plasma membrane.
R-SCE-171007. p38MAPK events.
R-SCE-5218921. VEGFR2 mediated cell proliferation.
R-SCE-5658442. Regulation of RAS by GAPs.
R-SCE-5673001. RAF/MAP kinase cascade.
R-SCE-5675221. Negative regulation of MAPK pathway.
R-SCE-8849471. PTK6 Regulates RHO GTPases, RAS GTPase and MAP kinases.

Names & Taxonomyi

Protein namesi
Recommended name:
Ras-like protein 1
Gene namesi
Name:RAS1
Ordered Locus Names:YOR101W
ORF Names:YOR3205W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR101W.
SGDiS000005627. RAS1.

Subcellular locationi

GO - Cellular componenti

  • intracellular Source: GOC
  • plasma membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi66 – 661A → T: Impaired GTPase activity, autophosphorylating activity.
Mutagenesisi68 – 681Q → L: Impaired GTPase activity.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 306306Ras-like protein 1PRO_0000030193Add
BLAST
Propeptidei307 – 3093Removed in mature formPRO_0000030194

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi305 – 3051S-palmitoyl cysteine1 Publication
Modified residuei306 – 3061Cysteine methyl ester1 Publication
Lipidationi306 – 3061S-farnesyl cysteine1 Publication

Post-translational modificationi

Farnesylated by RAM1-RAM2, which is required for targeting RAS1 to the cytoplasmic site of the endoplasmic reticulum, where proteolytic processing of the C-terminus by RCE1 and methylation of the resulting carboxyl group by STE14 occurs.2 Publications
Palmitoylated by the ERF2-SHR5 complex, which is required for proper plasma membrane localization of RAS1.By similarity

Keywords - PTMi

Lipoprotein, Methylation, Palmitate, Prenylation

Proteomic databases

MaxQBiP01119.

PTM databases

iPTMnetiP01119.
SwissPalmiP01119.

Interactioni

Protein-protein interaction databases

BioGridi34499. 50 interactions.
DIPiDIP-1041N.
IntActiP01119. 8 interactions.
MINTiMINT-520108.

Structurei

3D structure databases

ProteinModelPortaliP01119.
SMRiP01119. Positions 23-172.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi39 – 479Effector region

Sequence similaritiesi

Belongs to the small GTPase superfamily. Ras family.Curated

Phylogenomic databases

GeneTreeiENSGT00780000121849.
HOGENOMiHOG000233973.
InParanoidiP01119.
KOiK07827.
OMAiQKNSSAN.
OrthoDBiEOG092C4VY0.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR020849. Small_GTPase_Ras.
[Graphical view]
PANTHERiPTHR24070. PTHR24070. 1 hit.
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51421. RAS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P01119-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQGNKSTIRE YKIVVVGGGG VGKSALTIQF IQSYFVDEYD PTIEDSYRKQ
60 70 80 90 100
VVIDDKVSIL DILDTAGQEE YSAMREQYMR TGEGFLLVYS VTSRNSFDEL
110 120 130 140 150
LSYYQQIQRV KDSDYIPVVV VGNKLDLENE RQVSYEDGLR LAKQLNAPFL
160 170 180 190 200
ETSAKQAINV DEAFYSLIRL VRDDGGKYNS MNRQLDNTNE IRDSELTSSA
210 220 230 240 250
TADREKKNNG SYVLDNSLTN AGTGSSSKSA VNHNGETTKR TDEKNYVNQN
260 270 280 290 300
NNNEGNTKYS SNGNGNRSDI SRGNQNNALN SRSKQSAEPQ KNSSANARKE

SSGGCCIIC
Length:309
Mass (Da):34,347
Last modified:October 1, 1996 - v2
Checksum:iA70D720EB67E2F91
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti204 – 2041R → I in AAA34958 (PubMed:6365329).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K01970 Genomic DNA. Translation: AAA34958.1.
X00527 Genomic DNA. Translation: CAA25206.1.
X94335 Genomic DNA. Translation: CAA64023.1.
Z75009 Genomic DNA. Translation: CAA99298.1.
U32307 Genomic DNA. Translation: AAC49087.1.
BK006948 Genomic DNA. Translation: DAA10878.1.
PIRiS61661. TVBYR1.
RefSeqiNP_014744.1. NM_001183520.1.

Genome annotation databases

EnsemblFungiiYOR101W; YOR101W; YOR101W.
GeneIDi854268.
KEGGisce:YOR101W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K01970 Genomic DNA. Translation: AAA34958.1.
X00527 Genomic DNA. Translation: CAA25206.1.
X94335 Genomic DNA. Translation: CAA64023.1.
Z75009 Genomic DNA. Translation: CAA99298.1.
U32307 Genomic DNA. Translation: AAC49087.1.
BK006948 Genomic DNA. Translation: DAA10878.1.
PIRiS61661. TVBYR1.
RefSeqiNP_014744.1. NM_001183520.1.

3D structure databases

ProteinModelPortaliP01119.
SMRiP01119. Positions 23-172.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34499. 50 interactions.
DIPiDIP-1041N.
IntActiP01119. 8 interactions.
MINTiMINT-520108.

PTM databases

iPTMnetiP01119.
SwissPalmiP01119.

Proteomic databases

MaxQBiP01119.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOR101W; YOR101W; YOR101W.
GeneIDi854268.
KEGGisce:YOR101W.

Organism-specific databases

EuPathDBiFungiDB:YOR101W.
SGDiS000005627. RAS1.

Phylogenomic databases

GeneTreeiENSGT00780000121849.
HOGENOMiHOG000233973.
InParanoidiP01119.
KOiK07827.
OMAiQKNSSAN.
OrthoDBiEOG092C4VY0.

Enzyme and pathway databases

BioCyciYEAST:G3O-33634-MONOMER.
ReactomeiR-SCE-1445148. Translocation of GLUT4 to the plasma membrane.
R-SCE-171007. p38MAPK events.
R-SCE-5218921. VEGFR2 mediated cell proliferation.
R-SCE-5658442. Regulation of RAS by GAPs.
R-SCE-5673001. RAF/MAP kinase cascade.
R-SCE-5675221. Negative regulation of MAPK pathway.
R-SCE-8849471. PTK6 Regulates RHO GTPases, RAS GTPase and MAP kinases.

Miscellaneous databases

PROiP01119.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR020849. Small_GTPase_Ras.
[Graphical view]
PANTHERiPTHR24070. PTHR24070. 1 hit.
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51421. RAS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRAS1_YEAST
AccessioniPrimary (citable) accession number: P01119
Secondary accession number(s): D6W2G2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: October 1, 1996
Last modified: September 7, 2016
This is version 161 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2050 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.