P01117 (RASK_MSVKI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
GTPase KRas

Alternative name(s):
Ki-Ras
Transforming protein p21/K-Ras

Gene names

Name:	K-RAS
Synonyms:	KIS

Organism

Kirsten murine sarcoma virus

Taxonomic identifier

11808 [NCBI]

Taxonomic lineage

Viruses › Retro-transcribing viruses › Retroviridae › Orthoretrovirinae › Gammaretrovirus › Mammalian virus group › Replication defective viruses

Virus host

Mus musculus (Mouse) [TaxID: 10090]

Protein attributes

Sequence length	189 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Enzyme regulation	Alternate between an inactive form bound to GDP and an active form bound to GTP. Activated by a guanine nucleotide-exchange factor (GEF) and inactivated by a GTPase-activating protein (GAP).
Subcellular location	Host cell membrane; Lipid-anchor; Cytoplasmic side By similarity.
Miscellaneous	This p21 transforming protein was generated by a transduction of rodent cellular K-Ras-2 gene.
Sequence similarities	Belongs to the small GTPase superfamily. Ras family.

Ontologies

Keywords
Cellular component	Host cell membrane Host membrane Membrane
Disease	Oncogene
Ligand	GTP-binding Nucleotide-binding
PTM	Lipoprotein Methylation Palmitate Phosphoprotein Prenylation
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	small GTPase mediated signal transduction Inferred from electronic annotation. Source: InterPro
Cellular_component	host cell plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTPase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 186	186	GTPase KRas		PRO_0000030189
	Propeptide	187 – 189	3	Removed in mature form Probable		PRO_0000030190
Regions
	Nucleotide binding	10 – 17	8	GTP
	Nucleotide binding	57 – 61	5	GTP
	Nucleotide binding	116 – 119	4	GTP
	Motif	32 – 40	9	Effector region
Amino acid modifications
	Modified residue	59	1	Phosphothreonine; by autocatalysis Ref.3
	Modified residue	186	1	Cysteine methyl ester; by host Probable
	Lipidation	180	1	S-palmitoyl cysteine; by host Potential
	Lipidation	186	1	S-farnesyl cysteine; by host Probable

Sequences

	Sequence		Length	Mass (Da)
	P01117 [UniParc]. Last modified July 21, 1986. Version 1. Checksum: BBA3FB03EC374A4E	FASTA	189	21,715
	10 20 30 40 50 60 MTEYKLVVVG ASGVGKSALT IQLIQNHFVD EYDPTIQDSY RKQVVIDGET CLLDILDTTG 70 80 90 100 110 120 QEEYSAMRDQ YMRTGEGFLC VFAINNTKSF EDIHHYREQL KRVKDSEDVP MVLVGNKCDL 130 140 150 160 170 180 PSRTVDTKQA QELARSYGIP FIETSAKTRQ RVEDAFYTLV REIRQYRLKK ISKEEKTPGC VKIKKCVIM

References

[1]	"Nucleotide sequence of the oncogene encoding the p21 transforming protein of Kirsten murine sarcoma virus." Tsuchida N., Ryder T., Ohtsubo E. Science 217:937-939(1982) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[2]	"Ras genes." Barbacid M. Annu. Rev. Biochem. 56:779-827(1987) [PubMed] [Europe PMC] [Abstract] Cited for: REVIEW.
[3]	"Characterization of the phosphorylation sites and the surrounding amino acid sequences of the p21 transforming proteins coded for by the Harvey and Kirsten strains of murine sarcoma viruses." Shih T.Y., Stokes P.E., Smythers G.W., Dhar R., Oroszlan S. J. Biol. Chem. 257:11767-11773(1982) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 50-64, PHOSPHORYLATION AT THR-59.

Cross-references

Sequence databases
EMBL GenBank DDBJ	J02228 Genomic RNA. Translation: AAA46572.1.
PIR	TVMV2K. A01366.
3D structure databases
ProteinModelPortal	P01117.
SMR	P01117. Positions 1-166.
ModBase	Search...
Proteomic databases
PRIDE	P01117.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR005225. Small_GTP-bd_dom. IPR001806. Small_GTPase. IPR020849. Small_GTPase_Ras. [Graphical view]
PANTHER	PTHR24070. PTHR24070. 1 hit.
Pfam	PF00071. Ras. 1 hit. [Graphical view]
PRINTS	PR00449. RASTRNSFRMNG.
SMART	SM00173. RAS. 1 hit. [Graphical view]
TIGRFAMs	TIGR00231. small_GTP. 1 hit.
PROSITE	PS51421. RAS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RASK_MSVKI

Accession

Primary (citable) accession number: P01117

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	April 3, 2013
This is version 100 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program