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P01117

- RASK_MSVKI

UniProt

P01117 - RASK_MSVKI

Protein

GTPase KRas

Gene

K-RAS

Organism
Kirsten murine sarcoma virus
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 104 (01 Oct 2014)
      Sequence version 1 (21 Jul 1986)
      Previous versions | rss
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    Functioni

    Enzyme regulationi

    Alternates between an inactive form bound to GDP and an active form bound to GTP. Activated by a guanine nucleotide-exchange factor (GEF) and inactivated by a GTPase-activating protein (GAP).

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi10 – 178GTP
    Nucleotide bindingi57 – 615GTP
    Nucleotide bindingi116 – 1194GTP

    GO - Molecular functioni

    1. GTP binding Source: UniProtKB-KW

    GO - Biological processi

    1. GTP catabolic process Source: InterPro
    2. small GTPase mediated signal transduction Source: InterPro

    Keywords - Ligandi

    GTP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    GTPase KRas
    Alternative name(s):
    Ki-Ras
    Transforming protein p21/K-Ras
    Gene namesi
    Name:K-RAS
    Synonyms:KIS
    OrganismiKirsten murine sarcoma virus
    Taxonomic identifieri11808 [NCBI]
    Taxonomic lineageiVirusesRetro-transcribing virusesRetroviridaeOrthoretrovirinaeGammaretrovirus
    Virus hostiMus musculus (Mouse) [TaxID: 10090]

    Subcellular locationi

    Host cell membrane By similarity; Lipid-anchor By similarity; Cytoplasmic side By similarity

    GO - Cellular componenti

    1. host cell plasma membrane Source: UniProtKB-SubCell
    2. membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Host cell membrane, Host membrane, Membrane

    Pathology & Biotechi

    Keywords - Diseasei

    Oncogene

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 186186GTPase KRasPRO_0000030189Add
    BLAST
    Propeptidei187 – 1893Removed in mature formCuratedPRO_0000030190

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei59 – 591Phosphothreonine; by autocatalysis1 Publication
    Lipidationi180 – 1801S-palmitoyl cysteine; by hostSequence Analysis
    Modified residuei186 – 1861Cysteine methyl ester; by hostCurated
    Lipidationi186 – 1861S-farnesyl cysteine; by hostCurated

    Keywords - PTMi

    Lipoprotein, Methylation, Palmitate, Phosphoprotein, Prenylation

    Proteomic databases

    PRIDEiP01117.

    Structurei

    3D structure databases

    ProteinModelPortaliP01117.
    SMRiP01117. Positions 1-166.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi32 – 409Effector region

    Sequence similaritiesi

    Belongs to the small GTPase superfamily. Ras family.Curated

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    InterProiIPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR001806. Small_GTPase.
    IPR020849. Small_GTPase_Ras.
    [Graphical view]
    PANTHERiPTHR24070. PTHR24070. 1 hit.
    PfamiPF00071. Ras. 1 hit.
    [Graphical view]
    PRINTSiPR00449. RASTRNSFRMNG.
    SMARTiSM00173. RAS. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    TIGRFAMsiTIGR00231. small_GTP. 1 hit.
    PROSITEiPS51421. RAS. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P01117-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTEYKLVVVG ASGVGKSALT IQLIQNHFVD EYDPTIQDSY RKQVVIDGET    50
    CLLDILDTTG QEEYSAMRDQ YMRTGEGFLC VFAINNTKSF EDIHHYREQL 100
    KRVKDSEDVP MVLVGNKCDL PSRTVDTKQA QELARSYGIP FIETSAKTRQ 150
    RVEDAFYTLV REIRQYRLKK ISKEEKTPGC VKIKKCVIM 189
    Length:189
    Mass (Da):21,715
    Last modified:July 21, 1986 - v1
    Checksum:iBBA3FB03EC374A4E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J02228 Genomic RNA. Translation: AAA46572.1.
    PIRiA01366. TVMV2K.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J02228 Genomic RNA. Translation: AAA46572.1 .
    PIRi A01366. TVMV2K.

    3D structure databases

    ProteinModelPortali P01117.
    SMRi P01117. Positions 1-166.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi P01117.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    InterProi IPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR001806. Small_GTPase.
    IPR020849. Small_GTPase_Ras.
    [Graphical view ]
    PANTHERi PTHR24070. PTHR24070. 1 hit.
    Pfami PF00071. Ras. 1 hit.
    [Graphical view ]
    PRINTSi PR00449. RASTRNSFRMNG.
    SMARTi SM00173. RAS. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    TIGRFAMsi TIGR00231. small_GTP. 1 hit.
    PROSITEi PS51421. RAS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence of the oncogene encoding the p21 transforming protein of Kirsten murine sarcoma virus."
      Tsuchida N., Ryder T., Ohtsubo E.
      Science 217:937-939(1982) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    2. Cited for: REVIEW.
    3. "Characterization of the phosphorylation sites and the surrounding amino acid sequences of the p21 transforming proteins coded for by the Harvey and Kirsten strains of murine sarcoma viruses."
      Shih T.Y., Stokes P.E., Smythers G.W., Dhar R., Oroszlan S.
      J. Biol. Chem. 257:11767-11773(1982) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 50-64, PHOSPHORYLATION AT THR-59.

    Entry informationi

    Entry nameiRASK_MSVKI
    AccessioniPrimary (citable) accession number: P01117
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: July 21, 1986
    Last modified: October 1, 2014
    This is version 104 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    This p21 transforming protein was generated by a transduction of rodent cellular K-Ras-2 gene.

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3