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P01115 (RASH_MSVHA) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 97. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Transforming protein p29

Cleaved into the following chain:

  1. Transforming protein p21
Gene names
Name:H-RAS
OrganismHarvey murine sarcoma virus
Taxonomic identifier11807 [NCBI]
Taxonomic lineageVirusesRetro-transcribing virusesRetroviridaeOrthoretrovirinaeGammaretrovirus
Virus hostMus musculus (Mouse) [TaxID: 10090]

Protein attributes

Sequence length241 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Enzyme regulation

Alternates between an inactive form bound to GDP and an active form bound to GTP. Activated by a guanine nucleotide-exchange factor (GEF) and inactivated by a GTPase-activating protein (GAP).

Subcellular location

Host cell membrane; Lipid-anchor; Cytoplasmic side. Note: Inner surface of plasma membrane.

Miscellaneous

This p21 transforming protein was generated by a transduction of rodent cellular H-ras-1 gene.

Sequence similarities

Belongs to the small GTPase superfamily. Ras family.

Sequence caution

The sequence AAA46570.1 differs from that shown. Reason: Erroneous initiation.

The sequence CAA25322.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 238238Transforming protein p29
PRO_0000030183
Chain53 – 238186Transforming protein p21
PRO_0000030184
Propeptide239 – 2413Removed in mature form Probable
PRO_0000030185

Amino acid modifications

Modified residue2381Cysteine methyl ester; by host Probable
Lipidation2331S-palmitoyl cysteine; by host Potential
Lipidation2361S-palmitoyl cysteine; by host Potential
Lipidation2381S-farnesyl cysteine; by host Probable

Experimental info

Sequence conflict1741G → A in CAA25322. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P01115 [UniParc].

Last modified July 21, 1986. Version 1.
Checksum: 5A8983BFCD826C04

FASTA24126,328
        10         20         30         40         50         60 
MPAARAAPAA DEPMRDPVAP VRAPALPRPA PGAVAPASGG ARAPGLAAPV EAMTEYKLVV 

        70         80         90        100        110        120 
VGARGVGKSA LTIQLIQNHF VDEYDPTIED SYRKQVVIDG ETCLLDILDT TGQEEYSAMR 

       130        140        150        160        170        180 
DQYMRTGEGF LCVFAINNTK SFEDIHQYRE QIKRVKDSDD VPMVLVGNKC DLAGRTVESR 

       190        200        210        220        230        240 
QAQDLARSYG IPYIETSAKT RQGVEDAFYT LVREIRQHKL RKLNPPDESG PGCMSCKCVL 


S 

« Hide

References

[1]"Nucleotide sequence of the p21 transforming protein of Harvey murine sarcoma virus."
Dhar R., Ellis R.W., Shih T.Y., Oroszlan S., Shapiro B., Maizel J., Lowy D., Scolnick E.
Science 217:934-937(1982) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
[2]"An altered DNA sequence encompassing the ras gene of Harvey murine sarcoma virus."
Yasuda S., Furuichi M., Soeda E.
Nucleic Acids Res. 12:5583-5588(1984) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Ras genes."
Barbacid M.
Annu. Rev. Biochem. 56:779-827(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: REVIEW.
[4]"Localization of the src gene product of the Harvey strain of MSV to plasma membrane of transformed cells by electron microscopic immunocytochemistry."
Willingham M.C., Pastan I., Shih T.Y., Scolnick E.M.
Cell 19:1005-1014(1980) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X00740 Genomic DNA. Translation: CAA25322.1. Different initiation.
J02207 Genomic RNA. Translation: AAA46569.1.
J02207 Genomic RNA. Translation: AAA46570.1. Different initiation.
M24154 Genomic RNA. Translation: AAA46568.1.
PIRTVMV3H. A01363.

3D structure databases

ProteinModelPortalP01115.
SMRP01115. Positions 53-218.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEP01115.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.40.50.300. 1 hit.
InterProIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR020849. Small_GTPase_Ras.
[Graphical view]
PANTHERPTHR24070. PTHR24070. 1 hit.
PfamPF00071. Ras. 1 hit.
[Graphical view]
PRINTSPR00449. RASTRNSFRMNG.
SMARTSM00173. RAS. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
TIGRFAMsTIGR00231. small_GTP. 1 hit.
PROSITEPS51421. RAS. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRASH_MSVHA
AccessionPrimary (citable) accession number: P01115
Secondary accession number(s): O40604
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: April 16, 2014
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families