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P01115

- RASH_MSVHA

UniProt

P01115 - RASH_MSVHA

Protein

Transforming protein p29

Gene

H-RAS

Organism
Harvey murine sarcoma virus
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 98 (01 Oct 2014)
      Sequence version 1 (21 Jul 1986)
      Previous versions | rss
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    Functioni

    Enzyme regulationi

    Alternates between an inactive form bound to GDP and an active form bound to GTP. Activated by a guanine nucleotide-exchange factor (GEF) and inactivated by a GTPase-activating protein (GAP).

    GO - Molecular functioni

    1. GTP binding Source: UniProtKB-KW

    GO - Biological processi

    1. GTP catabolic process Source: InterPro
    2. small GTPase mediated signal transduction Source: InterPro

    Keywords - Ligandi

    GTP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Transforming protein p29
    Cleaved into the following chain:
    Gene namesi
    Name:H-RAS
    OrganismiHarvey murine sarcoma virus
    Taxonomic identifieri11807 [NCBI]
    Taxonomic lineageiVirusesRetro-transcribing virusesRetroviridaeOrthoretrovirinaeGammaretrovirus
    Virus hostiMus musculus (Mouse) [TaxID: 10090]

    Subcellular locationi

    Host cell membrane; Lipid-anchor; Cytoplasmic side
    Note: Inner surface of plasma membrane.

    GO - Cellular componenti

    1. host cell plasma membrane Source: UniProtKB-SubCell
    2. membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Host cell membrane, Host membrane, Membrane

    Pathology & Biotechi

    Keywords - Diseasei

    Oncogene

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 238238Transforming protein p29PRO_0000030183Add
    BLAST
    Chaini53 – 238186Transforming protein p21PRO_0000030184Add
    BLAST
    Propeptidei239 – 2413Removed in mature formCuratedPRO_0000030185

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Lipidationi233 – 2331S-palmitoyl cysteine; by hostSequence Analysis
    Lipidationi236 – 2361S-palmitoyl cysteine; by hostSequence Analysis
    Modified residuei238 – 2381Cysteine methyl ester; by hostCurated
    Lipidationi238 – 2381S-farnesyl cysteine; by hostCurated

    Keywords - PTMi

    Lipoprotein, Methylation, Palmitate, Prenylation

    Proteomic databases

    PRIDEiP01115.

    Structurei

    3D structure databases

    ProteinModelPortaliP01115.
    SMRiP01115. Positions 53-218.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the small GTPase superfamily. Ras family.Curated

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    InterProiIPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR001806. Small_GTPase.
    IPR020849. Small_GTPase_Ras.
    [Graphical view]
    PANTHERiPTHR24070. PTHR24070. 1 hit.
    PfamiPF00071. Ras. 1 hit.
    [Graphical view]
    PRINTSiPR00449. RASTRNSFRMNG.
    SMARTiSM00173. RAS. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    TIGRFAMsiTIGR00231. small_GTP. 1 hit.
    PROSITEiPS51421. RAS. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P01115-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPAARAAPAA DEPMRDPVAP VRAPALPRPA PGAVAPASGG ARAPGLAAPV    50
    EAMTEYKLVV VGARGVGKSA LTIQLIQNHF VDEYDPTIED SYRKQVVIDG 100
    ETCLLDILDT TGQEEYSAMR DQYMRTGEGF LCVFAINNTK SFEDIHQYRE 150
    QIKRVKDSDD VPMVLVGNKC DLAGRTVESR QAQDLARSYG IPYIETSAKT 200
    RQGVEDAFYT LVREIRQHKL RKLNPPDESG PGCMSCKCVL S 241
    Length:241
    Mass (Da):26,328
    Last modified:July 21, 1986 - v1
    Checksum:i5A8983BFCD826C04
    GO

    Sequence cautioni

    The sequence AAA46570.1 differs from that shown. Reason: Erroneous initiation.
    The sequence CAA25322.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti174 – 1741G → A in CAA25322. (PubMed:6087292)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X00740 Genomic DNA. Translation: CAA25322.1. Different initiation.
    J02207 Genomic RNA. Translation: AAA46569.1.
    J02207 Genomic RNA. Translation: AAA46570.1. Different initiation.
    M24154 Genomic RNA. Translation: AAA46568.1.
    PIRiA01363. TVMV3H.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X00740 Genomic DNA. Translation: CAA25322.1 . Different initiation.
    J02207 Genomic RNA. Translation: AAA46569.1 .
    J02207 Genomic RNA. Translation: AAA46570.1 . Different initiation.
    M24154 Genomic RNA. Translation: AAA46568.1 .
    PIRi A01363. TVMV3H.

    3D structure databases

    ProteinModelPortali P01115.
    SMRi P01115. Positions 53-218.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi P01115.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    InterProi IPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR001806. Small_GTPase.
    IPR020849. Small_GTPase_Ras.
    [Graphical view ]
    PANTHERi PTHR24070. PTHR24070. 1 hit.
    Pfami PF00071. Ras. 1 hit.
    [Graphical view ]
    PRINTSi PR00449. RASTRNSFRMNG.
    SMARTi SM00173. RAS. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    TIGRFAMsi TIGR00231. small_GTP. 1 hit.
    PROSITEi PS51421. RAS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence of the p21 transforming protein of Harvey murine sarcoma virus."
      Dhar R., Ellis R.W., Shih T.Y., Oroszlan S., Shapiro B., Maizel J., Lowy D., Scolnick E.
      Science 217:934-937(1982) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE.
    2. "An altered DNA sequence encompassing the ras gene of Harvey murine sarcoma virus."
      Yasuda S., Furuichi M., Soeda E.
      Nucleic Acids Res. 12:5583-5588(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. Cited for: REVIEW.
    4. "Localization of the src gene product of the Harvey strain of MSV to plasma membrane of transformed cells by electron microscopic immunocytochemistry."
      Willingham M.C., Pastan I., Shih T.Y., Scolnick E.M.
      Cell 19:1005-1014(1980) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.

    Entry informationi

    Entry nameiRASH_MSVHA
    AccessioniPrimary (citable) accession number: P01115
    Secondary accession number(s): O40604
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: July 21, 1986
    Last modified: October 1, 2014
    This is version 98 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    This p21 transforming protein was generated by a transduction of rodent cellular H-ras-1 gene.

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3