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Protein

Transforming protein p29

Gene

H-RAS

Organism
Harvey murine sarcoma virus
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Enzyme regulationi

Alternates between an inactive form bound to GDP and an active form bound to GTP. Activated by a guanine nucleotide-exchange factor (GEF) and inactivated by a GTPase-activating protein (GAP).

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Transforming protein p29
Cleaved into the following chain:
Gene namesi
Name:H-RAS
OrganismiHarvey murine sarcoma virus
Taxonomic identifieri11807 [NCBI]
Taxonomic lineageiVirusesRetro-transcribing virusesRetroviridaeOrthoretrovirinaeGammaretrovirus
Virus hostiMus musculus (Mouse) [TaxID: 10090]

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Host cell membrane, Host membrane, Membrane

Pathology & Biotechi

Keywords - Diseasei

Oncogene

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 238238Transforming protein p29PRO_0000030183Add
BLAST
Chaini53 – 238186Transforming protein p21PRO_0000030184Add
BLAST
Propeptidei239 – 2413Removed in mature formCuratedPRO_0000030185

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi233 – 2331S-palmitoyl cysteine; by hostSequence Analysis
Lipidationi236 – 2361S-palmitoyl cysteine; by hostSequence Analysis
Modified residuei238 – 2381Cysteine methyl ester; by hostCurated
Lipidationi238 – 2381S-farnesyl cysteine; by hostCurated

Keywords - PTMi

Lipoprotein, Methylation, Palmitate, Prenylation

Proteomic databases

PRIDEiP01115.

Structurei

3D structure databases

ProteinModelPortaliP01115.
SMRiP01115. Positions 53-218.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the small GTPase superfamily. Ras family.Curated

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR020849. Small_GTPase_Ras.
[Graphical view]
PANTHERiPTHR24070. PTHR24070. 1 hit.
PfamiPF00071. Ras. 1 hit.
[Graphical view]
PRINTSiPR00449. RASTRNSFRMNG.
SMARTiSM00173. RAS. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51421. RAS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P01115-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPAARAAPAA DEPMRDPVAP VRAPALPRPA PGAVAPASGG ARAPGLAAPV
60 70 80 90 100
EAMTEYKLVV VGARGVGKSA LTIQLIQNHF VDEYDPTIED SYRKQVVIDG
110 120 130 140 150
ETCLLDILDT TGQEEYSAMR DQYMRTGEGF LCVFAINNTK SFEDIHQYRE
160 170 180 190 200
QIKRVKDSDD VPMVLVGNKC DLAGRTVESR QAQDLARSYG IPYIETSAKT
210 220 230 240
RQGVEDAFYT LVREIRQHKL RKLNPPDESG PGCMSCKCVL S
Length:241
Mass (Da):26,328
Last modified:July 21, 1986 - v1
Checksum:i5A8983BFCD826C04
GO

Sequence cautioni

The sequence AAA46570.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence CAA25322.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti174 – 1741G → A in CAA25322 (PubMed:6087292).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X00740 Genomic DNA. Translation: CAA25322.1. Different initiation.
J02207 Genomic RNA. Translation: AAA46569.1.
J02207 Genomic RNA. Translation: AAA46570.1. Different initiation.
M24154 Genomic RNA. Translation: AAA46568.1.
PIRiA01363. TVMV3H.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X00740 Genomic DNA. Translation: CAA25322.1. Different initiation.
J02207 Genomic RNA. Translation: AAA46569.1.
J02207 Genomic RNA. Translation: AAA46570.1. Different initiation.
M24154 Genomic RNA. Translation: AAA46568.1.
PIRiA01363. TVMV3H.

3D structure databases

ProteinModelPortaliP01115.
SMRiP01115. Positions 53-218.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiP01115.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR020849. Small_GTPase_Ras.
[Graphical view]
PANTHERiPTHR24070. PTHR24070. 1 hit.
PfamiPF00071. Ras. 1 hit.
[Graphical view]
PRINTSiPR00449. RASTRNSFRMNG.
SMARTiSM00173. RAS. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51421. RAS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Nucleotide sequence of the p21 transforming protein of Harvey murine sarcoma virus."
    Dhar R., Ellis R.W., Shih T.Y., Oroszlan S., Shapiro B., Maizel J., Lowy D., Scolnick E.
    Science 217:934-937(1982) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
  2. "An altered DNA sequence encompassing the ras gene of Harvey murine sarcoma virus."
    Yasuda S., Furuichi M., Soeda E.
    Nucleic Acids Res. 12:5583-5588(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. Cited for: REVIEW.
  4. "Localization of the src gene product of the Harvey strain of MSV to plasma membrane of transformed cells by electron microscopic immunocytochemistry."
    Willingham M.C., Pastan I., Shih T.Y., Scolnick E.M.
    Cell 19:1005-1014(1980) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.

Entry informationi

Entry nameiRASH_MSVHA
AccessioniPrimary (citable) accession number: P01115
Secondary accession number(s): O40604
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: April 1, 2015
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Miscellaneous

This p21 transforming protein was generated by a transduction of rodent cellular H-ras-1 gene.

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.