P01115 (RASH_MSVHA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transforming protein p29

Cleaved into the following chain:

1. Transforming protein p21

Gene names

Name:

H-RAS

Organism

Harvey murine sarcoma virus

Taxonomic identifier

11807 [NCBI]

Taxonomic lineage

Viruses › Retro-transcribing viruses › Retroviridae › Orthoretrovirinae › Gammaretrovirus › Mammalian virus group › Replication defective viruses

Virus host

Mus musculus (Mouse) [TaxID: 10090]

Protein attributes

Sequence length	241 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Enzyme regulation	Alternate between an inactive form bound to GDP and an active form bound to GTP. Activated by a guanine nucleotide-exchange factor (GEF) and inactivated by a GTPase-activating protein (GAP).
Subcellular location	Host cell membrane; Lipid-anchor; Cytoplasmic side. Note: Inner surface of plasma membrane.
Miscellaneous	This p21 transforming protein was generated by a transduction of rodent cellular H-ras-1 gene.
Sequence similarities	Belongs to the small GTPase superfamily. Ras family.
Sequence caution	The sequence AAA46570.1 differs from that shown. Reason: Erroneous initiation. The sequence CAA25322.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Host cell membrane Host membrane Membrane
Disease	Oncogene
Ligand	GTP-binding Nucleotide-binding
PTM	Lipoprotein Methylation Palmitate Prenylation
Gene Ontology (GO)
Biological_process	small GTPase mediated signal transduction Inferred from electronic annotation. Source: InterPro
Cellular_component	host cell plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTPase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 238	238	Transforming protein p29		PRO_0000030183
	Chain	53 – 238	186	Transforming protein p21		PRO_0000030184
	Propeptide	239 – 241	3	Removed in mature form Probable		PRO_0000030185
Amino acid modifications
	Modified residue	238	1	Cysteine methyl ester; by host Probable
	Lipidation	233	1	S-palmitoyl cysteine; by host Potential
	Lipidation	236	1	S-palmitoyl cysteine; by host Potential
	Lipidation	238	1	S-farnesyl cysteine; by host Probable
Experimental info
	Sequence conflict	174	1	G → A in CAA25322. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P01115 [UniParc]. Last modified July 21, 1986. Version 1. Checksum: 5A8983BFCD826C04	FASTA	241	26,328
	10 20 30 40 50 60 MPAARAAPAA DEPMRDPVAP VRAPALPRPA PGAVAPASGG ARAPGLAAPV EAMTEYKLVV 70 80 90 100 110 120 VGARGVGKSA LTIQLIQNHF VDEYDPTIED SYRKQVVIDG ETCLLDILDT TGQEEYSAMR 130 140 150 160 170 180 DQYMRTGEGF LCVFAINNTK SFEDIHQYRE QIKRVKDSDD VPMVLVGNKC DLAGRTVESR 190 200 210 220 230 240 QAQDLARSYG IPYIETSAKT RQGVEDAFYT LVREIRQHKL RKLNPPDESG PGCMSCKCVL S

References

[1]	"Nucleotide sequence of the p21 transforming protein of Harvey murine sarcoma virus." Dhar R., Ellis R.W., Shih T.Y., Oroszlan S., Shapiro B., Maizel J., Lowy D., Scolnick E. Science 217:934-937(1982) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
[2]	"An altered DNA sequence encompassing the ras gene of Harvey murine sarcoma virus." Yasuda S., Furuichi M., Soeda E. Nucleic Acids Res. 12:5583-5588(1984) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Ras genes." Barbacid M. Annu. Rev. Biochem. 56:779-827(1987) [PubMed] [Europe PMC] [Abstract] Cited for: REVIEW.
[4]	"Localization of the src gene product of the Harvey strain of MSV to plasma membrane of transformed cells by electron microscopic immunocytochemistry." Willingham M.C., Pastan I., Shih T.Y., Scolnick E.M. Cell 19:1005-1014(1980) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X00740 Genomic DNA. Translation: CAA25322.1. Different initiation. J02207 Genomic RNA. Translation: AAA46569.1. J02207 Genomic RNA. Translation: AAA46570.1. Different initiation. M24154 Genomic RNA. Translation: AAA46568.1.
PIR	TVMV3H. A01363.
3D structure databases
ProteinModelPortal	P01115.
SMR	P01115. Positions 53-218.
ModBase	Search...
Proteomic databases
PRIDE	P01115.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR005225. Small_GTP-bd_dom. IPR001806. Small_GTPase. IPR020849. Small_GTPase_Ras. [Graphical view]
PANTHER	PTHR24070. PTHR24070. 1 hit.
Pfam	PF00071. Ras. 1 hit. [Graphical view]
PRINTS	PR00449. RASTRNSFRMNG.
SMART	SM00173. RAS. 1 hit. [Graphical view]
TIGRFAMs	TIGR00231. small_GTP. 1 hit.
PROSITE	PS51421. RAS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RASH_MSVHA

Accession

Primary (citable) accession number: P01115
Secondary accession number(s): O40604

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	April 3, 2013
This is version 94 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program