P01099 (PPR1A_RABIT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein phosphatase 1 regulatory subunit 1A

Alternative name(s):
Protein phosphatase inhibitor 1
Short name=I-1
Short name=IPP-1

Gene names

Name:	PPP1R1A
Synonyms:	IPP1

Organism

Oryctolagus cuniculus (Rabbit) [Reference proteome]

Taxonomic identifier

9986 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Lagomorpha › Leporidae › Oryctolagus

Protein attributes

Sequence length	166 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Inhibitor of protein-phosphatase 1. This protein may be important in hormonal control of glycogen metabolism. Hormones that elevate intracellular cAMP increase I-1 activity in many tissues. I-1 activation may impose cAMP control over proteins that are not directly phosphorylated by PKA. Following a rise in intracellular calcium, I-1 is inactivated by calcineurin (or PP2B). Does not inhibit type-2 phosphatases. Ref.3
Subunit structure	Interacts with PPP1R15A By similarity.
Post-translational modification	Phosphorylation of Thr-35 is required for activity By similarity.
Sequence similarities	Belongs to the protein phosphatase inhibitor 1 family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Glycogen metabolism
Molecular function	Protein phosphatase inhibitor
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	glycogen metabolic process Inferred from electronic annotation. Source: UniProtKB-KW signal transduction Inferred from electronic annotation. Source: InterPro
Molecular_function	protein phosphatase inhibitor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 166	166	Protein phosphatase 1 regulatory subunit 1A		PRO_0000071479
Regions
	Region	9 – 12	4	Essential for activity
	Region	42 – 54	13	Essential for activity Probable
	Region	143 – 166	24	Interaction with PPP1R15A By similarity
Amino acid modifications
	Modified residue	1	1	N-acetylmethionine Ref.1
	Modified residue	35	1	Phosphothreonine; by PKA By similarity Ref.1
	Modified residue	67	1	Phosphoserine Ref.1
Natural variations
	Natural variant	164 – 166	3	Missing in 40% of the molecules.

Sequences

	Sequence		Length	Mass (Da)
	P01099 [UniParc]. Last modified November 1, 1988. Version 1. Checksum: 0D26A5FE0C617C88	FASTA	166	18,032
	10 20 30 40 50 60 MEQDNSPRKI QFTVPLLEPH LDPEAAEQIR RRRPTPATLV LTSDQSSPEV DEDRIPNPLL 70 80 90 100 110 120 KPSLAMSPRQ RKKMTRTTPT MKELQMMVEH HLGQQEQGEE PEGAAEGTGA QESQPPGTPG 130 140 150 160 TGAESRLGPS ATAQKPAQPS PRAQERRGEE PSTAKTSQDS QGASAV

References

[1]	"Complete primary structure of protein phosphatase inhibitor-1 from rabbit skeletal muscle." Aitken A., Bilham T., Cohen P. Eur. J. Biochem. 126:235-246(1982) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE, ACETYLATION AT MET-1, PHOSPHORYLATION AT SER-67.
[2]	Aitken A. Submitted (OCT-1988) to UniProtKB Cited for: SEQUENCE REVISION TO 164.
[3]	"Isolation and characterisation of active fragments of protein phosphatase inhibitor-1 from rabbit skeletal muscle." Aitken A., Cohen P. FEBS Lett. 147:54-58(1982) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.

Cross-references

Sequence databases
PIR	PZRB1. A91120.
3D structure databases
DisProt	DP00325.
ModBase	Search...
Protein-protein interaction databases
STRING	9986.ENSOCUP00000011659.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
eggNOG	NOG48061.
HOGENOM	HOG000115665.
HOVERGEN	HBG096069.
OrthoDB	EOG4TMR3H.
Family and domain databases
InterPro	IPR008466. PPI_1DARPP-32. [Graphical view]
PANTHER	PTHR15417. PTHR15417. 1 hit.
Pfam	PF05395. DARPP-32. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PPR1A_RABIT

Accession

Primary (citable) accession number: P01099

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	November 1, 1988
Last modified:	April 3, 2013
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program