P01097 (ATIF_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ATPase inhibitor, mitochondrial

Gene names

Name:	INH1
Ordered Locus Names:	YDL181W
ORF Names:	D1305

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	85 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Forms a one-to-one complex with ATPase to inhibit the enzyme activity completely.
Subcellular location	Mitochondrion.
Miscellaneous	Present with 981 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the ATPase inhibitor family.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Coiled coil Transit peptide
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	negative regulation of ATPase activity Inferred from direct assay PubMed 12809520. Source: SGD negative regulation of nucleotide metabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	mitochondrion Inferred from direct assay PubMed 12809520PubMed 16823961. Source: SGD
Molecular_function	enzyme inhibitor activity Inferred from direct assay PubMed 12167646. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
YHR080C	P38800	1	EBI-8874,EBI-24597

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Transit peptide

1 – 22

22

Mitochondrion Ref.1 Ref.6

Chain

23 – 85

63

ATPase inhibitor, mitochondrial

PRO_0000002554

Regions

Coiled coil

41 – 84

44

Potential

Experimental info

Mutagenesis

41

1

K → Q or E: No loss of inhibitory activity. Ref.8

Secondary structure

1

.

.

.

.

.

85

Helix Strand Turn

Details...

Turn

24 – 28

5

Helix

38 – 57

20

Sequences

	Sequence		Length	Mass (Da)
	P01097 [UniParc]. Last modified August 1, 1990. Version 2. Checksum: E1AF73A47F63CE58	FASTA	85	9,870
	10 20 30 40 50 60 MLPRSALARS LQLQRGVAAR FYSEGSTGTP RGSGSEDSFV KRERATEDFF VRQREKEQLR 70 80 HLKEQLEKQR KKIDSLENKI DSMTK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Activation of ATP hydrolysis by an uncoupler in mutant mitochondria lacking an intrinsic ATPase inhibitor in yeast." Ichikawa N., Yoshida Y., Hashimoto T., Ogasawara N., Yoshikawa H., Imamoto F., Tagawa K. J. Biol. Chem. 265:6274-6278(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"New open reading frames, one of which is similar to the nifV gene of Azotobacter vinelandii, found on a 12.5 kbp fragment of chromosome IV of Saccharomyces cerevisiae." Verhasselt P., Voet M., Volckaert G. Yeast 11:961-966(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV." Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P. Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[6]	"Amino acid sequence of an intrinsic inhibitor of mitochondrial ATPase from yeast." Matsubara H., Hase T., Hashimoto T., Tagawa K. J. Biochem. 90:1159-1165(1981) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 23-85.
[7]	"Partial amino terminal sequence of the precursor of mitochondrial ATPase inhibitor protein synthesized with mRNA partially purified by gel permeation chromatography." Yoshida Y., Hashimoto T., Hase T., Matsubara H., Tagawa K. J. Biochem. 94:291-297(1983) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-28.
[8]	"Requirement for lysine-19 of the yeast mitochondrial ATPase inhibitor for the stability of the inactivated inhibitor-F1Fo complex at higher pH." Ichikawa N., Fujisaka R., Kuribayashi R. Biosci. Biotechnol. Biochem. 64:89-95(2000) [PubMed] [Europe PMC] [Abstract] Cited for: MUTAGENESIS OF LYS-41.
[9]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

D00443 Genomic DNA. Translation: BAA00344.1.
X83276 Genomic DNA. Translation: CAA58265.1.
Z74229 Genomic DNA. Translation: CAA98755.1.
AY558374 Genomic DNA. Translation: AAS56700.1.
BK006938 Genomic DNA. Translation: DAA11681.1.

PIR

IWBY. A35231.

RefSeq

NP_010100.1. NM_001180241.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3ZIA	X-ray	2.50	J/T	23-85	[»]

ProteinModelPortal

P01097.

SMR

P01097. Positions 30-65.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-2985N.

IntAct

P01097. 5 interactions.

MINT

MINT-434735.

STRING

4932.YDL181W.

Proteomic databases

PaxDb

P01097.

PeptideAtlas

P01097.

PRIDE

P01097.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YDL181W; YDL181W; YDL181W.

GeneID

851347.

KEGG

sce:YDL181W.

Organism-specific databases

CYGD

YDL181w.

SGD

S000002340. INH1.

Phylogenomic databases

eggNOG

NOG84090.

GeneTree

ENSGT00390000016182.

HOGENOM

HOG000183772.

OMA

KRERATE.

OrthoDB

EOG4SXRPD.

Enzyme and pathway databases

BioCyc

YEAST:G3O-29568-MONOMER.

Gene expression databases

Genevestigator

P01097.

GermOnline

YDL181W. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR007648. ATPase_inhibitor_IATP_mt.
[Graphical view]

Pfam

PF04568. IATP. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

968432.

Entry information

Entry name

ATIF_YEAST

Accession

Primary (citable) accession number: P01097
Secondary accession number(s): D6VRH1

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	August 1, 1990
Last modified:	May 29, 2013
This is version 122 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program