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P01097 (ATIF_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 129. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
ATPase inhibitor, mitochondrial
Gene names
Name:INH1
Ordered Locus Names:YDL181W
ORF Names:D1305
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length85 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Forms a one-to-one complex with ATPase to inhibit the enzyme activity completely.

Subcellular location

Mitochondrion.

Miscellaneous

Present with 981 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the ATPase inhibitor family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

YHR080CP388001EBI-8874,EBI-24597

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 2222Mitochondrion Ref.1 Ref.6
Chain23 – 8563ATPase inhibitor, mitochondrial
PRO_0000002554

Regions

Coiled coil41 – 8444 Potential

Experimental info

Mutagenesis411K → Q or E: No loss of inhibitory activity. Ref.8

Secondary structure

..... 85
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P01097 [UniParc].

Last modified August 1, 1990. Version 2.
Checksum: E1AF73A47F63CE58

FASTA859,870
        10         20         30         40         50         60 
MLPRSALARS LQLQRGVAAR FYSEGSTGTP RGSGSEDSFV KRERATEDFF VRQREKEQLR 

        70         80 
HLKEQLEKQR KKIDSLENKI DSMTK 

« Hide

References

« Hide 'large scale' references
[1]"Activation of ATP hydrolysis by an uncoupler in mutant mitochondria lacking an intrinsic ATPase inhibitor in yeast."
Ichikawa N., Yoshida Y., Hashimoto T., Ogasawara N., Yoshikawa H., Imamoto F., Tagawa K.
J. Biol. Chem. 265:6274-6278(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"New open reading frames, one of which is similar to the nifV gene of Azotobacter vinelandii, found on a 12.5 kbp fragment of chromosome IV of Saccharomyces cerevisiae."
Verhasselt P., Voet M., Volckaert G.
Yeast 11:961-966(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[3]"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. expand/collapse author list , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[6]"Amino acid sequence of an intrinsic inhibitor of mitochondrial ATPase from yeast."
Matsubara H., Hase T., Hashimoto T., Tagawa K.
J. Biochem. 90:1159-1165(1981) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 23-85.
[7]"Partial amino terminal sequence of the precursor of mitochondrial ATPase inhibitor protein synthesized with mRNA partially purified by gel permeation chromatography."
Yoshida Y., Hashimoto T., Hase T., Matsubara H., Tagawa K.
J. Biochem. 94:291-297(1983) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 1-28.
[8]"Requirement for lysine-19 of the yeast mitochondrial ATPase inhibitor for the stability of the inactivated inhibitor-F1Fo complex at higher pH."
Ichikawa N., Fujisaka R., Kuribayashi R.
Biosci. Biotechnol. Biochem. 64:89-95(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: MUTAGENESIS OF LYS-41.
[9]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D00443 Genomic DNA. Translation: BAA00344.1.
X83276 Genomic DNA. Translation: CAA58265.1.
Z74229 Genomic DNA. Translation: CAA98755.1.
AY558374 Genomic DNA. Translation: AAS56700.1.
BK006938 Genomic DNA. Translation: DAA11681.1.
PIRIWBY. A35231.
RefSeqNP_010100.1. NM_001180241.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3ZIAX-ray2.50J/T23-85[»]
ProteinModelPortalP01097.
SMRP01097. Positions 23-58.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid31863. 48 interactions.
DIPDIP-2985N.
IntActP01097. 5 interactions.
MINTMINT-434735.
STRING4932.YDL181W.

Proteomic databases

PaxDbP01097.
PeptideAtlasP01097.
PRIDEP01097.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYDL181W; YDL181W; YDL181W.
GeneID851347.
KEGGsce:YDL181W.

Organism-specific databases

CYGDYDL181w.
SGDS000002340. INH1.

Phylogenomic databases

eggNOGNOG84090.
GeneTreeENSGT00390000016182.
HOGENOMHOG000183772.
OMAYAIRQRE.
OrthoDBEOG7MSN2Q.

Enzyme and pathway databases

BioCycYEAST:G3O-29568-MONOMER.

Gene expression databases

GenevestigatorP01097.

Family and domain databases

InterProIPR007648. ATPase_inhibitor_IATP_mt.
[Graphical view]
PfamPF04568. IATP. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio968432.

Entry information

Entry nameATIF_YEAST
AccessionPrimary (citable) accession number: P01097
Secondary accession number(s): D6VRH1
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: August 1, 1990
Last modified: April 16, 2014
This is version 129 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome IV

Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references