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P01097

- ATIF_YEAST

UniProt

P01097 - ATIF_YEAST

Protein

ATPase inhibitor, mitochondrial

Gene

INH1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Forms a one-to-one complex with ATPase to inhibit the enzyme activity completely.

    GO - Molecular functioni

    1. ATPase inhibitor activity Source: SGD
    2. enzyme inhibitor activity Source: SGD

    GO - Biological processi

    1. negative regulation of ATPase activity Source: SGD
    2. negative regulation of nucleotide metabolic process Source: InterPro

    Enzyme and pathway databases

    BioCyciYEAST:G3O-29568-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    ATPase inhibitor, mitochondrial
    Gene namesi
    Name:INH1
    Ordered Locus Names:YDL181W
    ORF Names:D1305
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome IV

    Organism-specific databases

    CYGDiYDL181w.
    SGDiS000002340. INH1.

    Subcellular locationi

    GO - Cellular componenti

    1. mitochondrion Source: SGD

    Keywords - Cellular componenti

    Mitochondrion

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi41 – 411K → Q or E: No loss of inhibitory activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 2222Mitochondrion2 PublicationsAdd
    BLAST
    Chaini23 – 8563ATPase inhibitor, mitochondrialPRO_0000002554Add
    BLAST

    Proteomic databases

    MaxQBiP01097.
    PaxDbiP01097.
    PeptideAtlasiP01097.
    PRIDEiP01097.

    Expressioni

    Gene expression databases

    GenevestigatoriP01097.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    YHR080CP388001EBI-8874,EBI-24597

    Protein-protein interaction databases

    BioGridi31863. 48 interactions.
    DIPiDIP-2985N.
    IntActiP01097. 5 interactions.
    MINTiMINT-434735.
    STRINGi4932.YDL181W.

    Structurei

    Secondary structure

    1
    85
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Turni24 – 285
    Helixi38 – 5720

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3ZIAX-ray2.50J/T23-85[»]
    ProteinModelPortaliP01097.
    SMRiP01097. Positions 23-58.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili41 – 8444Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Belongs to the ATPase inhibitor family.Curated

    Keywords - Domaini

    Coiled coil, Transit peptide

    Phylogenomic databases

    eggNOGiNOG84090.
    GeneTreeiENSGT00390000016182.
    HOGENOMiHOG000183772.
    OMAiKRERATE.
    OrthoDBiEOG7MSN2Q.

    Family and domain databases

    InterProiIPR007648. ATPase_inhibitor_IATP_mt.
    [Graphical view]
    PfamiPF04568. IATP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P01097-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLPRSALARS LQLQRGVAAR FYSEGSTGTP RGSGSEDSFV KRERATEDFF   50
    VRQREKEQLR HLKEQLEKQR KKIDSLENKI DSMTK 85
    Length:85
    Mass (Da):9,870
    Last modified:August 1, 1990 - v2
    Checksum:iE1AF73A47F63CE58
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D00443 Genomic DNA. Translation: BAA00344.1.
    X83276 Genomic DNA. Translation: CAA58265.1.
    Z74229 Genomic DNA. Translation: CAA98755.1.
    AY558374 Genomic DNA. Translation: AAS56700.1.
    BK006938 Genomic DNA. Translation: DAA11681.1.
    PIRiA35231. IWBY.
    RefSeqiNP_010100.1. NM_001180241.1.

    Genome annotation databases

    EnsemblFungiiYDL181W; YDL181W; YDL181W.
    GeneIDi851347.
    KEGGisce:YDL181W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D00443 Genomic DNA. Translation: BAA00344.1 .
    X83276 Genomic DNA. Translation: CAA58265.1 .
    Z74229 Genomic DNA. Translation: CAA98755.1 .
    AY558374 Genomic DNA. Translation: AAS56700.1 .
    BK006938 Genomic DNA. Translation: DAA11681.1 .
    PIRi A35231. IWBY.
    RefSeqi NP_010100.1. NM_001180241.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3ZIA X-ray 2.50 J/T 23-85 [» ]
    ProteinModelPortali P01097.
    SMRi P01097. Positions 23-58.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31863. 48 interactions.
    DIPi DIP-2985N.
    IntActi P01097. 5 interactions.
    MINTi MINT-434735.
    STRINGi 4932.YDL181W.

    Proteomic databases

    MaxQBi P01097.
    PaxDbi P01097.
    PeptideAtlasi P01097.
    PRIDEi P01097.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YDL181W ; YDL181W ; YDL181W .
    GeneIDi 851347.
    KEGGi sce:YDL181W.

    Organism-specific databases

    CYGDi YDL181w.
    SGDi S000002340. INH1.

    Phylogenomic databases

    eggNOGi NOG84090.
    GeneTreei ENSGT00390000016182.
    HOGENOMi HOG000183772.
    OMAi KRERATE.
    OrthoDBi EOG7MSN2Q.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-29568-MONOMER.

    Miscellaneous databases

    NextBioi 968432.

    Gene expression databases

    Genevestigatori P01097.

    Family and domain databases

    InterProi IPR007648. ATPase_inhibitor_IATP_mt.
    [Graphical view ]
    Pfami PF04568. IATP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Activation of ATP hydrolysis by an uncoupler in mutant mitochondria lacking an intrinsic ATPase inhibitor in yeast."
      Ichikawa N., Yoshida Y., Hashimoto T., Ogasawara N., Yoshikawa H., Imamoto F., Tagawa K.
      J. Biol. Chem. 265:6274-6278(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "New open reading frames, one of which is similar to the nifV gene of Azotobacter vinelandii, found on a 12.5 kbp fragment of chromosome IV of Saccharomyces cerevisiae."
      Verhasselt P., Voet M., Volckaert G.
      Yeast 11:961-966(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 96604 / S288c / FY1679.
    3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
      Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
      , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
      Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    6. "Amino acid sequence of an intrinsic inhibitor of mitochondrial ATPase from yeast."
      Matsubara H., Hase T., Hashimoto T., Tagawa K.
      J. Biochem. 90:1159-1165(1981) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 23-85.
    7. "Partial amino terminal sequence of the precursor of mitochondrial ATPase inhibitor protein synthesized with mRNA partially purified by gel permeation chromatography."
      Yoshida Y., Hashimoto T., Hase T., Matsubara H., Tagawa K.
      J. Biochem. 94:291-297(1983) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 1-28.
    8. "Requirement for lysine-19 of the yeast mitochondrial ATPase inhibitor for the stability of the inactivated inhibitor-F1Fo complex at higher pH."
      Ichikawa N., Fujisaka R., Kuribayashi R.
      Biosci. Biotechnol. Biochem. 64:89-95(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: MUTAGENESIS OF LYS-41.
    9. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiATIF_YEAST
    AccessioniPrimary (citable) accession number: P01097
    Secondary accession number(s): D6VRH1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: August 1, 1990
    Last modified: October 1, 2014
    This is version 132 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 981 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome IV
      Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

    External Data

    Dasty 3