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Protein

Protease A inhibitor 3

Gene

PAI3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Specific and potent inhibitor for yeast aspartic protease A (yscA). The proteinase acts as a folding template stabilizing the helical conformation in the inhibitor, which results in the potent and specific blockage of the proteolytic activity.

GO - Molecular functioni

  • aspartic-type endopeptidase inhibitor activity Source: CAFA
  • endopeptidase inhibitor activity Source: CAFA
  • protease binding Source: CAFA

GO - Biological processi

  • negative regulation of endopeptidase activity Source: CAFA
  • protein catabolic process in the vacuole Source: SGD

Keywordsi

Molecular functionAspartic protease inhibitor, Protease inhibitor

Enzyme and pathway databases

BioCyciYEAST:G3O-32862-MONOMER

Protein family/group databases

MEROPSiI34.001

Names & Taxonomyi

Protein namesi
Recommended name:
Protease A inhibitor 3
Alternative name(s):
Proteinase inhibitor I(A)3
Proteinase yscA-inhibitor
Saccharopepsin inhibitor
Gene namesi
Name:PAI3
Ordered Locus Names:YMR174C
ORF Names:YM8010.04C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YMR174C
SGDiS000004786 PAI3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001959011 – 68Protease A inhibitor 3Add BLAST68

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionine1 Publication1

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP01094
PaxDbiP01094
PRIDEiP01094

Interactioni

GO - Molecular functioni

  • protease binding Source: CAFA

Protein-protein interaction databases

BioGridi35352, 85 interactors
IntActiP01094, 4 interactors
MINTiP01094
STRINGi4932.YMR174C

Structurei

Secondary structure

168
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi4 – 30Combined sources27

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1DP5X-ray2.20B1-68[»]
1DPJX-ray1.80B2-34[»]
1G0VX-ray2.00B1-31[»]
DisProtiDP00179
ProteinModelPortaliP01094
SMRiP01094
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP01094

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 32Inhibitory domainAdd BLAST32

Sequence similaritiesi

Phylogenomic databases

InParanoidiP01094
OrthoDBiEOG092C5VSY

Family and domain databases

InterProiView protein in InterPro
IPR008956 IA3_dom_sf
IPR019507 Pai3
PfamiView protein in Pfam
PF10466 Inhibitor_I34, 1 hit
SUPFAMiSSF48686 SSF48686, 1 hit

Sequencei

Sequence statusi: Complete.

P01094-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNTDQQKVSE IFQSSKEKLQ GDAKVVSDAF KKMASQDKDG KTTDADESEK
60
HNYQEQYNKL KGAGHKKE
Length:68
Mass (Da):7,707
Last modified:July 21, 1986 - v1
Checksum:i8295BF7AF3E4A01A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X60050 Genomic DNA Translation: CAA42650.1
Z49808 Genomic DNA Translation: CAA89907.1
AY558120 Genomic DNA Translation: AAS56446.1
BK006946 Genomic DNA Translation: DAA10070.1
PIRiA01334 IABY3
RefSeqiNP_013899.1, NM_001182680.1

Genome annotation databases

EnsemblFungiiYMR174C; YMR174C; YMR174C
GeneIDi855212
KEGGisce:YMR174C

Similar proteinsi

Entry informationi

Entry nameiIPA3_YEAST
AccessioniPrimary (citable) accession number: P01094
Secondary accession number(s): D6VZZ6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: May 23, 2018
This is version 159 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

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