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Protein

Protease A inhibitor 3

Gene

PAI3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Specific and potent inhibitor for yeast aspartic protease A (yscA). The proteinase acts as a folding template stabilizing the helical conformation in the inhibitor, which results in the potent and specific blockage of the proteolytic activity.

GO - Molecular functioni

GO - Biological processi

  • protein catabolic process in the vacuole Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Aspartic protease inhibitor, Protease inhibitor

Enzyme and pathway databases

BioCyciYEAST:G3O-32862-MONOMER.

Protein family/group databases

MEROPSiI34.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Protease A inhibitor 3
Alternative name(s):
Proteinase inhibitor I(A)3
Proteinase yscA-inhibitor
Saccharopepsin inhibitor
Gene namesi
Name:PAI3
Ordered Locus Names:YMR174C
ORF Names:YM8010.04C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YMR174C.
SGDiS000004786. PAI3.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 6868Protease A inhibitor 3PRO_0000195901Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionine1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP01094.
PRIDEiP01094.

Interactioni

Protein-protein interaction databases

BioGridi35352. 11 interactions.
IntActiP01094. 4 interactions.
MINTiMINT-382885.

Structurei

Secondary structure

1
68
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi4 – 3027Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1DP5X-ray2.20B1-68[»]
1DPJX-ray1.80B2-34[»]
1G0VX-ray2.00B1-31[»]
DisProtiDP00179.
DP00586.
ProteinModelPortaliP01094.
SMRiP01094. Positions 3-31.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP01094.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 3232Inhibitory domainAdd
BLAST

Sequence similaritiesi

Phylogenomic databases

InParanoidiP01094.
OrthoDBiEOG7XPZK7.

Family and domain databases

Gene3Di1.20.5.140. 1 hit.
InterProiIPR023223. DNA_polIII_IA3_alpha-helix.
IPR008956. IA3_dom.
IPR019507. Pai3.
[Graphical view]
PfamiPF10466. Inhibitor_I34. 1 hit.
[Graphical view]
SUPFAMiSSF48686. SSF48686. 1 hit.

Sequencei

Sequence statusi: Complete.

P01094-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNTDQQKVSE IFQSSKEKLQ GDAKVVSDAF KKMASQDKDG KTTDADESEK
60
HNYQEQYNKL KGAGHKKE
Length:68
Mass (Da):7,707
Last modified:July 21, 1986 - v1
Checksum:i8295BF7AF3E4A01A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X60050 Genomic DNA. Translation: CAA42650.1.
Z49808 Genomic DNA. Translation: CAA89907.1.
AY558120 Genomic DNA. Translation: AAS56446.1.
BK006946 Genomic DNA. Translation: DAA10070.1.
PIRiA01334. IABY3.
RefSeqiNP_013899.1. NM_001182680.1.

Genome annotation databases

EnsemblFungiiYMR174C; YMR174C; YMR174C.
GeneIDi855212.
KEGGisce:YMR174C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X60050 Genomic DNA. Translation: CAA42650.1.
Z49808 Genomic DNA. Translation: CAA89907.1.
AY558120 Genomic DNA. Translation: AAS56446.1.
BK006946 Genomic DNA. Translation: DAA10070.1.
PIRiA01334. IABY3.
RefSeqiNP_013899.1. NM_001182680.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1DP5X-ray2.20B1-68[»]
1DPJX-ray1.80B2-34[»]
1G0VX-ray2.00B1-31[»]
DisProtiDP00179.
DP00586.
ProteinModelPortaliP01094.
SMRiP01094. Positions 3-31.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35352. 11 interactions.
IntActiP01094. 4 interactions.
MINTiMINT-382885.

Protein family/group databases

MEROPSiI34.001.

Proteomic databases

MaxQBiP01094.
PRIDEiP01094.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYMR174C; YMR174C; YMR174C.
GeneIDi855212.
KEGGisce:YMR174C.

Organism-specific databases

EuPathDBiFungiDB:YMR174C.
SGDiS000004786. PAI3.

Phylogenomic databases

InParanoidiP01094.
OrthoDBiEOG7XPZK7.

Enzyme and pathway databases

BioCyciYEAST:G3O-32862-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP01094.
PROiP01094.

Family and domain databases

Gene3Di1.20.5.140. 1 hit.
InterProiIPR023223. DNA_polIII_IA3_alpha-helix.
IPR008956. IA3_dom.
IPR019507. Pai3.
[Graphical view]
PfamiPF10466. Inhibitor_I34. 1 hit.
[Graphical view]
SUPFAMiSSF48686. SSF48686. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The amino acid sequence of proteinase A inhibitor 3 from baker's yeast."
    Biedermann K., Montali U., Martin B., Svendsen I., Ottesen M.
    Carlsberg Res. Commun. 45:225-235(1980)
    Cited for: PROTEIN SEQUENCE, ACETYLATION AT MET-1.
  2. "The proteinase yscA-inhibitor, IA3, gene. Studies of cytoplasmic proteinase inhibitor deficiency on yeast physiology."
    Schu P., Wolf D.H.
    FEBS Lett. 283:78-84(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  6. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  7. "The aspartic proteinase from Saccharomyces cerevisiae folds its own inhibitor into a helix."
    Li M., Phylip L.H., Lees W.E., Winther J.R., Dunn B.M., Wlodawer A., Kay J., Gustchina A.
    Nat. Struct. Biol. 7:113-117(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 2-32.

Entry informationi

Entry nameiIPA3_YEAST
AccessioniPrimary (citable) accession number: P01094
Secondary accession number(s): D6VZZ6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: July 6, 2016
This is version 144 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.