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Protein

Protease A inhibitor 3

Gene

PAI3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Specific and potent inhibitor for yeast aspartic protease A (yscA). The proteinase acts as a folding template stabilizing the helical conformation in the inhibitor, which results in the potent and specific blockage of the proteolytic activity.

GO - Molecular functioni

  • aspartic-type endopeptidase inhibitor activity Source: CAFA
  • endopeptidase inhibitor activity Source: CAFA
  • protease binding Source: CAFA

GO - Biological processi

  • negative regulation of endopeptidase activity Source: CAFA
  • protein catabolic process in the vacuole Source: SGD

Keywordsi

Molecular functionAspartic protease inhibitor, Protease inhibitor

Enzyme and pathway databases

BioCyciYEAST:G3O-32862-MONOMER.

Protein family/group databases

MEROPSiI34.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Protease A inhibitor 3
Alternative name(s):
Proteinase inhibitor I(A)3
Proteinase yscA-inhibitor
Saccharopepsin inhibitor
Gene namesi
Name:PAI3
Ordered Locus Names:YMR174C
ORF Names:YM8010.04C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YMR174C.
SGDiS000004786. PAI3.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001959011 – 68Protease A inhibitor 3Add BLAST68

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionine1 Publication1

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP01094.
PRIDEiP01094.

Interactioni

GO - Molecular functioni

  • protease binding Source: CAFA

Protein-protein interaction databases

BioGridi35352. 84 interactors.
IntActiP01094. 8 interactors.
MINTiMINT-382885.
STRINGi4932.YMR174C.

Structurei

Secondary structure

168
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi4 – 30Combined sources27

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1DP5X-ray2.20B1-68[»]
1DPJX-ray1.80B2-34[»]
1G0VX-ray2.00B1-31[»]
DisProtiDP00179.
ProteinModelPortaliP01094.
SMRiP01094.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP01094.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 32Inhibitory domainAdd BLAST32

Sequence similaritiesi

Phylogenomic databases

InParanoidiP01094.
OrthoDBiEOG092C5VSY.

Family and domain databases

InterProiView protein in InterPro
IPR008956. IA3_dom.
IPR019507. Pai3.
PfamiView protein in Pfam
PF10466. Inhibitor_I34. 1 hit.
SUPFAMiSSF48686. SSF48686. 1 hit.

Sequencei

Sequence statusi: Complete.

P01094-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNTDQQKVSE IFQSSKEKLQ GDAKVVSDAF KKMASQDKDG KTTDADESEK
60
HNYQEQYNKL KGAGHKKE
Length:68
Mass (Da):7,707
Last modified:July 21, 1986 - v1
Checksum:i8295BF7AF3E4A01A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X60050 Genomic DNA. Translation: CAA42650.1.
Z49808 Genomic DNA. Translation: CAA89907.1.
AY558120 Genomic DNA. Translation: AAS56446.1.
BK006946 Genomic DNA. Translation: DAA10070.1.
PIRiA01334. IABY3.
RefSeqiNP_013899.1. NM_001182680.1.

Genome annotation databases

EnsemblFungiiYMR174C; YMR174C; YMR174C.
GeneIDi855212.
KEGGisce:YMR174C.

Similar proteinsi

Entry informationi

Entry nameiIPA3_YEAST
AccessioniPrimary (citable) accession number: P01094
Secondary accession number(s): D6VZZ6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: September 27, 2017
This is version 152 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names