Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

2S albumin

Gene
N/A
Organism
Ricinus communis (Castor bean)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

2S seed storage proteins.

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Seed storage protein, Storage protein

Names & Taxonomyi

Protein namesi
Recommended name:
2S albumin
Alternative name(s):
Allergen: Ric c 1/3
Cleaved into the following 4 chains:
Alternative name(s):
4.7 kDa napin-like protein small chain
CB-1A small chain
RS1A
Alternative name(s):
CB-1A large chain
RL1
Alternative name(s):
2S albumin small chain
4 kDa napin-like protein small chain
RS2B
Alternative name(s):
2S albumin large chain
7.3 kDa napin-like protein large chain
RL2
OrganismiRicinus communis (Castor bean)
Taxonomic identifieri3988 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsMalpighialesEuphorbiaceaeAcalyphoideaeAcalypheaeRicinus

Pathology & Biotechi

Allergenic propertiesi

Causes an allergic reaction in human.

Biotechnological usei

Ric C 3 constitutes the peptidic component of the immunomodulator Inmunoferon.

Keywords - Diseasei

Allergen

Protein family/group databases

Allergomei3467. Ric c 1.0101.
614. Ric c 1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2424Sequence analysisAdd
BLAST
Propeptidei25 – 3511PRO_0000032162Add
BLAST
Chaini36 – 7641Allergen Ric c 3 small chainPRO_0000041857Add
BLAST
Propeptidei77 – 86101 PublicationPRO_0000041858
Chaini87 – 15367Allergen Ric c 3 large chainPRO_0000041859Add
BLAST
Propeptidei154 – 1563PRO_0000041860
Chaini157 – 19034Allergen Ric c 1 small chainPRO_0000032163Add
BLAST
Propeptidei191 – 1933PRO_0000032164
Chaini194 – 25865Allergen Ric c 1 large chainPRO_0000032165Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi49 ↔ 108Interchain (between Ric c 3 small and Ric c 3 large chains)1 Publication
Disulfide bondi61 ↔ 97Interchain (between Ric c 3 small and Ric c 3 large chains)1 Publication
Modified residuei69 – 691Phosphoserine1 Publication
Modified residuei87 – 871Pyrrolidone carboxylic acidCurated
Disulfide bondi98 ↔ 1451 Publication
Disulfide bondi110 ↔ 1491 Publication
Disulfide bondi162 ↔ 212Interchain (between Ric c 1 small and Ric c 1 large chains)By similarity
Disulfide bondi175 ↔ 201Interchain (between Ric c 1 small and Ric c 1 large chains)By similarity
Modified residuei194 – 1941Pyrrolidone carboxylic acid1 Publication
Disulfide bondi202 ↔ 249By similarity
Disulfide bondi214 ↔ 256By similarity

Post-translational modificationi

The N-terminus of both large chains is blocked.
The C-terminus of the allergen Ric c 1 and allergen Ric c 3 small chains are heterogeneous and the length of the chains can vary from 33 to 36 amino acids and from 36 to 40 amino acids respectively.

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Phosphoprotein, Pyrrolidone carboxylic acid

PTM databases

iPTMnetiP01089.

Expressioni

Developmental stagei

Expressed in ripening seeds during testa formation and desiccation.1 Publication

Interactioni

Subunit structurei

The 2 mature proteins consist of heterodimers of a small and a large chain; disulfide-linked.1 Publication

Structurei

Secondary structure

1
258
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi44 – 485Combined sources
Helixi49 – 535Combined sources
Helixi61 – 666Combined sources
Beta strandi67 – 693Combined sources
Beta strandi84 – 863Combined sources
Beta strandi88 – 903Combined sources
Helixi92 – 10110Combined sources
Helixi108 – 12316Combined sources
Helixi132 – 14514Combined sources
Turni149 – 1513Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1PSYNMR-A33-156[»]
ProteinModelPortaliP01089.
SMRiP01089. Positions 33-156.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP01089.

Family & Domainsi

Sequence similaritiesi

Belongs to the 2S seed storage albumins family.Curated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di1.10.120.10. 1 hit.
InterProiIPR016140. Bifunc_inhib/LTP/seed_store.
IPR000617. Napin.
IPR013771. Trypsin/amylase_inhib.
[Graphical view]
PfamiPF00234. Tryp_alpha_amyl. 2 hits.
[Graphical view]
PRINTSiPR00496. NAPIN.
SMARTiSM00499. AAI. 2 hits.
[Graphical view]
SUPFAMiSSF47699. SSF47699. 2 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P01089-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKLIPTIAL VSVLLFIIAN ASFAYRTTIT TIEIDESKGE REGSSSQQCR
60 70 80 90 100
QEVQRKDLSS CERYLRQSSS RRSPGEEVLR MPGDENQQQE SQQLQQCCNQ
110 120 130 140 150
VKQVRDECQC EAIKYIAEDQ IQQGQLHGEE SERVAQRAGE IVSSCGVRCM
160 170 180 190 200
RQTRTNPSQQ GCRGQIQEQQ NLRQCQEYIK QQVSGQGPRR SDNQERSLRG
210 220 230 240 250
CCDHLKQMQS QCRCEGLRQA IEQQQSQGQL QGQDVFEAFR TAANLPSMCG

VSPTECRF
Length:258
Mass (Da):29,290
Last modified:November 1, 1990 - v2
Checksum:i27874CFC50E41072
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti194 – 1941Q → E AA sequence (PubMed:8980648).Curated
Sequence conflicti222 – 2221E → Q AA sequence (PubMed:7174664).Curated
Sequence conflicti226 – 2294Missing AA sequence (PubMed:7174664).Curated
Sequence conflicti234 – 2341D → N AA sequence (PubMed:7174664).Curated
Sequence conflicti255 – 2551E → Q AA sequence (PubMed:7174664).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti74 – 741P → T.1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X54158 Genomic DNA. Translation: CAA38097.1.
PIRiS11499. RZCS.
RefSeqiXP_002522854.1. XM_002522808.2.
XP_002522855.1. XM_002522809.1.

Genome annotation databases

GeneIDi8280732.
8280733.
KEGGircu:RCOM_0184110.
rcu:RCOM_0184120.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X54158 Genomic DNA. Translation: CAA38097.1.
PIRiS11499. RZCS.
RefSeqiXP_002522854.1. XM_002522808.2.
XP_002522855.1. XM_002522809.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1PSYNMR-A33-156[»]
ProteinModelPortaliP01089.
SMRiP01089. Positions 33-156.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

Allergomei3467. Ric c 1.0101.
614. Ric c 1.

PTM databases

iPTMnetiP01089.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi8280732.
8280733.
KEGGircu:RCOM_0184110.
rcu:RCOM_0184120.

Miscellaneous databases

EvolutionaryTraceiP01089.

Family and domain databases

Gene3Di1.10.120.10. 1 hit.
InterProiIPR016140. Bifunc_inhib/LTP/seed_store.
IPR000617. Napin.
IPR013771. Trypsin/amylase_inhib.
[Graphical view]
PfamiPF00234. Tryp_alpha_amyl. 2 hits.
[Graphical view]
PRINTSiPR00496. NAPIN.
SMARTiSM00499. AAI. 2 hits.
[Graphical view]
SUPFAMiSSF47699. SSF47699. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry namei2SS_RICCO
AccessioniPrimary (citable) accession number: P01089
Secondary accession number(s): Q9S872, Q9S873, Q9S874
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: November 1, 1990
Last modified: September 7, 2016
This is version 104 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

The allergen Ric c 1 small chain acts as a calmodulin (CaM) antagonist that inhibits CaM-dependent myosin light chain kinase with IC50= 0.25 µM.

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. Allergens
    Nomenclature of allergens and list of entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.