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Protein

Alpha-amylase/trypsin inhibitor

Gene
N/A
Organism
Eleusine coracana (Indian finger millet) (Ragi)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

May play a protective role against endo- and exogenous hydrolytic activities in the Ragi seeds.

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Alpha-amylase inhibitor, Protease inhibitor, Serine protease inhibitor

Protein family/group databases

MEROPSiI06.003.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-amylase/trypsin inhibitor
Short name:
RBI
Alternative name(s):
RATI
OrganismiEleusine coracana (Indian finger millet) (Ragi)
Taxonomic identifieri4511 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaePACMAD cladeChloridoideaeCynodonteaeEleusininaeEleusine

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 122122Alpha-amylase/trypsin inhibitorPRO_0000070490Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi6 ↔ 55Combined sources1 Publication
Disulfide bondi20 ↔ 44Combined sources1 Publication
Disulfide bondi29 ↔ 85Combined sources1 Publication
Disulfide bondi45 ↔ 103Combined sources1 Publication
Disulfide bondi57 ↔ 114Combined sources1 Publication

Keywords - PTMi

Disulfide bond

Expressioni

Tissue specificityi

Seeds.

Interactioni

Protein-protein interaction databases

MINTiMINT-1537215.

Structurei

Secondary structure

1
122
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi2 – 54Combined sources
Turni9 – 113Combined sources
Turni16 – 183Combined sources
Helixi19 – 2911Combined sources
Beta strandi32 – 354Combined sources
Helixi37 – 5014Combined sources
Turni53 – 553Combined sources
Helixi56 – 649Combined sources
Helixi87 – 937Combined sources
Helixi94 – 963Combined sources
Turni100 – 1034Combined sources
Beta strandi110 – 1123Combined sources
Beta strandi115 – 1173Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1B1UX-ray2.20A1-122[»]
1BIPNMR-A1-122[»]
1TMQX-ray2.50B1-117[»]
ProteinModelPortaliP01087.
SMRiP01087. Positions 3-119.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP01087.

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di1.10.120.10. 1 hit.
InterProiIPR006106. Allergen/soft/tryp_amyl_inhib.
IPR006105. Allergen/tryp_amyl_inhib_CS.
IPR016140. Bifunc_inhib/LTP/seed_store.
IPR013771. Trypsin/amylase_inhib.
[Graphical view]
PfamiPF00234. Tryp_alpha_amyl. 1 hit.
[Graphical view]
PRINTSiPR00808. AMLASEINHBTR.
SMARTiSM00499. AAI. 1 hit.
[Graphical view]
SUPFAMiSSF47699. SSF47699. 1 hit.
PROSITEiPS00426. CEREAL_TRYP_AMYL_INH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P01087-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
SVGTSCIPGM AIPHNPLDSC RWYVSTRTCG VGPRLATQEM KARCCRQLEA
60 70 80 90 100
IPAYCRCEAV RILMDGVVTP SGQHEGRLLQ DLPGCPRQVQ RAFAPKLVTE
110 120
VECNLATIHG GPFCLSLLGA GE
Length:122
Mass (Da):13,138
Last modified:July 15, 1998 - v2
Checksum:iC8ED7A01CD470E17
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti25 – 262ST → AK.
Natural varianti28 – 281T → A.
Natural varianti70 – 701P → S.

Sequence databases

PIRiA01326. WIILAI.

Cross-referencesi

Sequence databases

PIRiA01326. WIILAI.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1B1UX-ray2.20A1-122[»]
1BIPNMR-A1-122[»]
1TMQX-ray2.50B1-117[»]
ProteinModelPortaliP01087.
SMRiP01087. Positions 3-119.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-1537215.

Protein family/group databases

MEROPSiI06.003.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP01087.

Family and domain databases

Gene3Di1.10.120.10. 1 hit.
InterProiIPR006106. Allergen/soft/tryp_amyl_inhib.
IPR006105. Allergen/tryp_amyl_inhib_CS.
IPR016140. Bifunc_inhib/LTP/seed_store.
IPR013771. Trypsin/amylase_inhib.
[Graphical view]
PfamiPF00234. Tryp_alpha_amyl. 1 hit.
[Graphical view]
PRINTSiPR00808. AMLASEINHBTR.
SMARTiSM00499. AAI. 1 hit.
[Graphical view]
SUPFAMiSSF47699. SSF47699. 1 hit.
PROSITEiPS00426. CEREAL_TRYP_AMYL_INH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiIAAT_ELECO
AccessioniPrimary (citable) accession number: P01087
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 15, 1998
Last modified: June 8, 2016
This is version 103 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.