Skip Header

Contribute Send feedback
Read comments (?) or add your own

P01085 (IAA1_WHEAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 77. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Alpha-amylase inhibitor 0.19
Alternative name(s):
0.19 alpha-AI
Short name=0.19 AI
OrganismTriticum aestivum (Wheat)
Taxonomic identifier4565 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Pooideae › Triticeae › Triticum

Protein attributes

Sequence length124 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Alpha-amylase inhibitor.

Subunit structure

Homodimer.

Subcellular location

Secreted.

Tissue specificity

Endosperm.

Post-translational modification

The disulfide bonds are essential for the inhibitor activity.

Sequence similarities

Belongs to the protease inhibitor I6 (cereal trypsin/alpha-amylase inhibitor) family. [View classification]

Ontologies

Keywords
   Cellular componentSecreted
   Molecular functionAlpha-amylase inhibitor
   PTMDisulfide bond
   Technical term3D-structure
Direct protein sequencing
Gene Ontology (GO)
   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionalpha-amylase inhibitor activity

Inferred from electronic annotation. Source: UniProtKB-KW

serine-type endopeptidase inhibitor activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 124124Alpha-amylase inhibitor 0.19
PRO_0000070484

Amino acid modifications

Disulfide bond6 ↔ 52
Disulfide bond20 ↔ 41
Disulfide bond28 ↔ 83
Disulfide bond42 ↔ 99
Disulfide bond54 ↔ 115

Secondary structure

1 .................... 124
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P01085 [UniParc].

Last modified July 21, 1986. Version 1.
Checksum: 97A8FB07D5A7788B

FASTA12413,337
        10         20         30         40         50         60 
SGPWMCYPGQ AFQVPALPAC RPLLRLQCNG SQVPEAVLRD CCQQLAHISE WCRCGALYSM 

        70         80         90        100        110        120 
LDSMYKEHGA QEGQAGTGAF PRCRREVVKL TAASITAVCR LPIVVDASGD GAYVCKDVAA 


YPDA

« Hide

References

[1]"Complete amino acid sequence of an alpha-amylase inhibitor in wheat kernel (0.19-inhibitor)."
Maeda K., Wakabayashi S., Matsubara H.
Biochim. Biophys. Acta 828:213-221(1985) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE.
[2]"Overexpression in Escherichia coli of chemically synthesized gene for active 0.19 alpha-amylase inhibitor from wheat kernel."
Okuda M., Satoh T., Sakurai N., Shibuya K., Kaji H., Samejima T.
J. Biochem. 122:918-926(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Kernel.
[3]"Tertiary and quaternary structures of 0.19 alpha-amylase inhibitor from wheat kernel determined by X-ray analysis at 2.06-A resolution."
Oda Y., Matsunaga T., Fukuyama K., Miyazaki T., Morimoto T.
Biochemistry 36:13503-13511(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.06 ANGSTROMS).

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AB003682 mRNA. Translation: BAA20139.1.
PIRWIWTA1. A01324.
UniGeneTa.24102.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1HSSX-ray2.06A/B/C/D1-124[»]
ProteinModelPortalP01085.
ModBaseSearch...

Protein family/group databases

Allergome8186. Tri a 28.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Organism-specific databases

GrameneP01085.

Family and domain databases

Gene3D1.10.120.10. 1 hit.
InterProIPR006106. Allergen/soft/tryp_amyl_inhib.
IPR006105. Allergen/tryp_amyl_inhib_CS.
IPR016140. Bifunc_inhib/LTP/seed_store.
IPR013771. Trypsin/amylase_inhib.
[Graphical view]
PfamPF00234. Tryp_alpha_amyl. 1 hit.
[Graphical view]
PRINTSPR00808. AMLASEINHBTR.
SMARTSM00499. AAI. 1 hit.
[Graphical view]
SUPFAMSSF47699. Bifunc_inhib/LTP/seed_store. 1 hit.
PROSITEPS00426. CEREAL_TRYP_AMYL_INH. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP01085.

Entry information

Entry nameIAA1_WHEAT
AccessionPrimary (citable) accession number: P01085
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: April 3, 2013
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents