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P01077 (IMEP_STRNI) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 64. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Metalloproteinase inhibitor
Gene names
Name:smpI
OrganismStreptomyces nigrescens
Taxonomic identifier1920 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomyces

Protein attributes

Sequence length131 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Inhibits microbial metallo-proteinases, such as thermolysin, but not serine, thiol, or carboxyl proteinases.

Miscellaneous

Thermolysin slowly cleaves only the peptide bond following Cys-93.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2929 Ref.2
Chain30 – 131102Metalloproteinase inhibitor
PRO_0000021509

Amino acid modifications

Disulfide bond33 ↔ 39 Ref.2
Disulfide bond93 ↔ 98 Ref.2

Secondary structure

.................... 131
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P01077 [UniParc].

Last modified August 1, 1992. Version 2.
Checksum: D204C23A57A67BD1

FASTA13113,186
        10         20         30         40         50         60 
MVRKRALGLA GSALTLVLGA VGFTAPAQAA PSCPAGSLCT YSGTGLSGAR TVIPASDMEK 

        70         80         90        100        110        120 
AGTDGVKLPA SARSFANGTH FTLRYGPARK VTCVRFPCYQ YATVGKVAPG AQLRSLPSPG 

       130 
ATVTVGQDLG D 

« Hide

References

[1]"Nucleotide sequence of the gene for a metalloproteinase inhibitor of Streptomyces nigrescens (SMPI)."
Tanaka K., Aoki H., Oda K., Murao S., Saito H., Takahashi H.
Nucleic Acids Res. 18:6433-6433(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Amino acid sequence of Streptomyces metallo-proteinase inhibitor from Streptomyces nigrescens TK-23."
Murai H., Hara S., Ikenaka T., Oda K., Murao S.
J. Biochem. 97:173-180(1985) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 30-131, DISULFIDE BONDS.
Strain: TK-23.
[3]"NMR structure of the Streptomyces metalloproteinase inhibitor, SMPI, isolated from Streptomyces nigrescens TK-23: another example of an ancestral beta gamma-crystallin precursor structure."
Ohno A., Tate S., Seeram S.S., Hiraga K., Swindells M.B., Oda K., Kainosho M.
J. Mol. Biol. 282:421-433(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY NMR.
Strain: TK-23.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D00671 Genomic DNA. Translation: BAA00574.1.
PIRZYSMN. S12615.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1BHUNMR-A30-131[»]
ProteinModelPortalP01077.
SMRP01077. Positions 30-131.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

MEROPSI36.001.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D2.60.20.30. 1 hit.
InterProIPR015791. Antimic/Inh_G_crystallin-like.
IPR011024. G_crystallin-rel.
IPR007141. Prot_inh_SMPI.
[Graphical view]
PfamPF03995. Inhibitor_I36. 1 hit.
[Graphical view]
SUPFAMSSF49695. SSF49695. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceP01077.

Entry information

Entry nameIMEP_STRNI
AccessionPrimary (citable) accession number: P01077
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: August 1, 1992
Last modified: February 19, 2014
This is version 64 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references