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P01077

- IMEP_STRNI

UniProt

P01077 - IMEP_STRNI

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Protein

Metalloproteinase inhibitor

Gene
smpI
Organism
Streptomyces nigrescens
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Inhibits microbial metallo-proteinases, such as thermolysin, but not serine, thiol, or carboxyl proteinases.

GO - Molecular functioni

  1. metalloendopeptidase inhibitor activity Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Metalloenzyme inhibitor, Metalloprotease inhibitor, Protease inhibitor

Protein family/group databases

MEROPSiI36.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Metalloproteinase inhibitor
Gene namesi
Name:smpI
OrganismiStreptomyces nigrescens
Taxonomic identifieri1920 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomyces

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 29291 PublicationAdd
BLAST
Chaini30 – 131102Metalloproteinase inhibitorPRO_0000021509Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi33 ↔ 391 Publication
Disulfide bondi93 ↔ 981 Publication

Keywords - PTMi

Disulfide bond

Structurei

Secondary structure

1
131
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi35 – 384
Beta strandi41 – 466
Beta strandi53 – 564
Helixi58 – 614
Beta strandi74 – 763
Beta strandi82 – 876
Beta strandi94 – 963
Turni108 – 1114
Beta strandi112 – 1187
Beta strandi121 – 1299

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1BHUNMR-A30-131[»]
ProteinModelPortaliP01077.
SMRiP01077. Positions 30-131.

Miscellaneous databases

EvolutionaryTraceiP01077.

Family & Domainsi

Keywords - Domaini

Signal

Family and domain databases

Gene3Di2.60.20.30. 1 hit.
InterProiIPR015791. Antimic/Inh_G_crystallin-like.
IPR011024. G_crystallin-rel.
IPR007141. Prot_inh_SMPI.
[Graphical view]
PfamiPF03995. Inhibitor_I36. 1 hit.
[Graphical view]
SUPFAMiSSF49695. SSF49695. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P01077-1 [UniParc]FASTAAdd to Basket

« Hide

MVRKRALGLA GSALTLVLGA VGFTAPAQAA PSCPAGSLCT YSGTGLSGAR    50
TVIPASDMEK AGTDGVKLPA SARSFANGTH FTLRYGPARK VTCVRFPCYQ 100
YATVGKVAPG AQLRSLPSPG ATVTVGQDLG D 131
Length:131
Mass (Da):13,186
Last modified:August 1, 1992 - v2
Checksum:iD204C23A57A67BD1
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D00671 Genomic DNA. Translation: BAA00574.1.
PIRiS12615. ZYSMN.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D00671 Genomic DNA. Translation: BAA00574.1 .
PIRi S12615. ZYSMN.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1BHU NMR - A 30-131 [» ]
ProteinModelPortali P01077.
SMRi P01077. Positions 30-131.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

MEROPSi I36.001.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Miscellaneous databases

EvolutionaryTracei P01077.

Family and domain databases

Gene3Di 2.60.20.30. 1 hit.
InterProi IPR015791. Antimic/Inh_G_crystallin-like.
IPR011024. G_crystallin-rel.
IPR007141. Prot_inh_SMPI.
[Graphical view ]
Pfami PF03995. Inhibitor_I36. 1 hit.
[Graphical view ]
SUPFAMi SSF49695. SSF49695. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Nucleotide sequence of the gene for a metalloproteinase inhibitor of Streptomyces nigrescens (SMPI)."
    Tanaka K., Aoki H., Oda K., Murao S., Saito H., Takahashi H.
    Nucleic Acids Res. 18:6433-6433(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Amino acid sequence of Streptomyces metallo-proteinase inhibitor from Streptomyces nigrescens TK-23."
    Murai H., Hara S., Ikenaka T., Oda K., Murao S.
    J. Biochem. 97:173-180(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 30-131, DISULFIDE BONDS.
    Strain: TK-23.
  3. "NMR structure of the Streptomyces metalloproteinase inhibitor, SMPI, isolated from Streptomyces nigrescens TK-23: another example of an ancestral beta gamma-crystallin precursor structure."
    Ohno A., Tate S., Seeram S.S., Hiraga K., Swindells M.B., Oda K., Kainosho M.
    J. Mol. Biol. 282:421-433(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR.
    Strain: TK-23.

Entry informationi

Entry nameiIMEP_STRNI
AccessioniPrimary (citable) accession number: P01077
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: August 1, 1992
Last modified: April 16, 2014
This is version 65 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Thermolysin slowly cleaves only the peptide bond following Cys-93.

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

External Data

Dasty 3