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P01077

- IMEP_STRNI

UniProt

P01077 - IMEP_STRNI

Protein

Metalloproteinase inhibitor

Gene

smpI

Organism
Streptomyces nigrescens
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Inhibits microbial metallo-proteinases, such as thermolysin, but not serine, thiol, or carboxyl proteinases.

    GO - Molecular functioni

    1. metalloendopeptidase inhibitor activity Source: UniProtKB-KW

    Keywords - Molecular functioni

    Metalloenzyme inhibitor, Metalloprotease inhibitor, Protease inhibitor

    Protein family/group databases

    MEROPSiI36.001.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Metalloproteinase inhibitor
    Gene namesi
    Name:smpI
    OrganismiStreptomyces nigrescens
    Taxonomic identifieri1920 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomyces

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 29291 PublicationAdd
    BLAST
    Chaini30 – 131102Metalloproteinase inhibitorPRO_0000021509Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi33 ↔ 391 Publication
    Disulfide bondi93 ↔ 981 Publication

    Keywords - PTMi

    Disulfide bond

    Structurei

    Secondary structure

    1
    131
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi35 – 384
    Beta strandi41 – 466
    Beta strandi53 – 564
    Helixi58 – 614
    Beta strandi74 – 763
    Beta strandi82 – 876
    Beta strandi94 – 963
    Turni108 – 1114
    Beta strandi112 – 1187
    Beta strandi121 – 1299

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1BHUNMR-A30-131[»]
    ProteinModelPortaliP01077.
    SMRiP01077. Positions 30-131.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP01077.

    Family & Domainsi

    Keywords - Domaini

    Signal

    Family and domain databases

    Gene3Di2.60.20.30. 1 hit.
    InterProiIPR015791. Antimic/Inh_G_crystallin-like.
    IPR011024. G_crystallin-rel.
    IPR007141. Prot_inh_SMPI.
    [Graphical view]
    PfamiPF03995. Inhibitor_I36. 1 hit.
    [Graphical view]
    SUPFAMiSSF49695. SSF49695. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P01077-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVRKRALGLA GSALTLVLGA VGFTAPAQAA PSCPAGSLCT YSGTGLSGAR    50
    TVIPASDMEK AGTDGVKLPA SARSFANGTH FTLRYGPARK VTCVRFPCYQ 100
    YATVGKVAPG AQLRSLPSPG ATVTVGQDLG D 131
    Length:131
    Mass (Da):13,186
    Last modified:August 1, 1992 - v2
    Checksum:iD204C23A57A67BD1
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D00671 Genomic DNA. Translation: BAA00574.1.
    PIRiS12615. ZYSMN.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D00671 Genomic DNA. Translation: BAA00574.1 .
    PIRi S12615. ZYSMN.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1BHU NMR - A 30-131 [» ]
    ProteinModelPortali P01077.
    SMRi P01077. Positions 30-131.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi I36.001.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei P01077.

    Family and domain databases

    Gene3Di 2.60.20.30. 1 hit.
    InterProi IPR015791. Antimic/Inh_G_crystallin-like.
    IPR011024. G_crystallin-rel.
    IPR007141. Prot_inh_SMPI.
    [Graphical view ]
    Pfami PF03995. Inhibitor_I36. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49695. SSF49695. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence of the gene for a metalloproteinase inhibitor of Streptomyces nigrescens (SMPI)."
      Tanaka K., Aoki H., Oda K., Murao S., Saito H., Takahashi H.
      Nucleic Acids Res. 18:6433-6433(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Amino acid sequence of Streptomyces metallo-proteinase inhibitor from Streptomyces nigrescens TK-23."
      Murai H., Hara S., Ikenaka T., Oda K., Murao S.
      J. Biochem. 97:173-180(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 30-131, DISULFIDE BONDS.
      Strain: TK-23.
    3. "NMR structure of the Streptomyces metalloproteinase inhibitor, SMPI, isolated from Streptomyces nigrescens TK-23: another example of an ancestral beta gamma-crystallin precursor structure."
      Ohno A., Tate S., Seeram S.S., Hiraga K., Swindells M.B., Oda K., Kainosho M.
      J. Mol. Biol. 282:421-433(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR.
      Strain: TK-23.

    Entry informationi

    Entry nameiIMEP_STRNI
    AccessioniPrimary (citable) accession number: P01077
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: August 1, 1992
    Last modified: October 1, 2014
    This is version 66 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Thermolysin slowly cleaves only the peptide bond following Cys-93.

    Keywords - Technical termi

    3D-structure, Direct protein sequencing

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references

    External Data

    Dasty 3