P01077 (IMEP_STRNI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Metalloproteinase inhibitor

Gene names

Name:

smpI

Organism

Streptomyces nigrescens

Taxonomic identifier

1920 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	131 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Inhibits microbial metallo-proteinases, such as thermolysin, but not serine, thiol, or carboxyl proteinases.
Miscellaneous	Thermolysin slowly cleaves only the peptide bond following Cys-93.

Ontologies

Keywords
Domain	Signal
Molecular function	Metalloenzyme inhibitor Metalloprotease inhibitor Protease inhibitor
PTM	Disulfide bond
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Molecular_function	metalloendopeptidase inhibitor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 29

29

Ref.2

Chain

30 – 131

102

Metalloproteinase inhibitor

PRO_0000021509

Amino acid modifications

Disulfide bond

33 ↔ 39

Ref.2

Disulfide bond

93 ↔ 98

Ref.2

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

131

Helix Strand Turn

Details...

Beta strand

35 – 38

4

Beta strand

41 – 46

6

Beta strand

53 – 56

4

Helix

58 – 61

4

Beta strand

74 – 76

3

Beta strand

82 – 87

6

Beta strand

94 – 96

3

Turn

108 – 111

4

Beta strand

112 – 118

7

Beta strand

121 – 129

9

Sequences

	Sequence		Length	Mass (Da)
	P01077 [UniParc]. Last modified August 1, 1992. Version 2. Checksum: D204C23A57A67BD1	FASTA	131	13,186
	10 20 30 40 50 60 MVRKRALGLA GSALTLVLGA VGFTAPAQAA PSCPAGSLCT YSGTGLSGAR TVIPASDMEK 70 80 90 100 110 120 AGTDGVKLPA SARSFANGTH FTLRYGPARK VTCVRFPCYQ YATVGKVAPG AQLRSLPSPG 130 ATVTVGQDLG D

References

[1]	"Nucleotide sequence of the gene for a metalloproteinase inhibitor of Streptomyces nigrescens (SMPI)." Tanaka K., Aoki H., Oda K., Murao S., Saito H., Takahashi H. Nucleic Acids Res. 18:6433-6433(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Amino acid sequence of Streptomyces metallo-proteinase inhibitor from Streptomyces nigrescens TK-23." Murai H., Hara S., Ikenaka T., Oda K., Murao S. J. Biochem. 97:173-180(1985) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 30-131, DISULFIDE BONDS. Strain: TK-23.
[3]	"NMR structure of the Streptomyces metalloproteinase inhibitor, SMPI, isolated from Streptomyces nigrescens TK-23: another example of an ancestral beta gamma-crystallin precursor structure." Ohno A., Tate S., Seeram S.S., Hiraga K., Swindells M.B., Oda K., Kainosho M. J. Mol. Biol. 282:421-433(1998) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY NMR. Strain: TK-23.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

D00671 Genomic DNA. Translation: BAA00574.1.

PIR

ZYSMN. S12615.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1BHU	NMR	-	A	30-131	[»]

ProteinModelPortal

P01077.

SMR

P01077. Positions 30-131.

ModBase

Search...

Protein family/group databases

MEROPS

I36.001.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

Gene3D

2.60.20.30. 1 hit.

InterPro

IPR015791. Antimic/Inh_G_crystallin-like.
IPR011024. G_crystallin-rel.
IPR007141. Prot_inh_SMPI.
[Graphical view]

Pfam

PF03995. Inhibitor_I36. 1 hit.
[Graphical view]

SUPFAM

SSF49695. G_crystallin_SF. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

P01077.

Entry information

Entry name

IMEP_STRNI

Accession

Primary (citable) accession number: P01077

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	August 1, 1992
Last modified:	May 1, 2013
This is version 62 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program