Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Metalloproteinase inhibitor

Gene

smpI

Organism
Streptomyces nigrescens
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Inhibits microbial metallo-proteinases, such as thermolysin, but not serine, thiol, or carboxyl proteinases.

GO - Molecular functioni

  1. metalloendopeptidase inhibitor activity Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Metalloenzyme inhibitor, Metalloprotease inhibitor, Protease inhibitor

Protein family/group databases

MEROPSiI36.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Metalloproteinase inhibitor
Gene namesi
Name:smpI
OrganismiStreptomyces nigrescens
Taxonomic identifieri1920 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomyces

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 29291 PublicationAdd
BLAST
Chaini30 – 131102Metalloproteinase inhibitorPRO_0000021509Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi33 ↔ 391 Publication
Disulfide bondi93 ↔ 981 Publication

Keywords - PTMi

Disulfide bond

Structurei

Secondary structure

1
131
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi35 – 384Combined sources
Beta strandi41 – 466Combined sources
Beta strandi53 – 564Combined sources
Helixi58 – 614Combined sources
Beta strandi74 – 763Combined sources
Beta strandi82 – 876Combined sources
Beta strandi94 – 963Combined sources
Turni108 – 1114Combined sources
Beta strandi112 – 1187Combined sources
Beta strandi121 – 1299Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1BHUNMR-A30-131[»]
ProteinModelPortaliP01077.
SMRiP01077. Positions 30-131.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP01077.

Family & Domainsi

Keywords - Domaini

Signal

Family and domain databases

Gene3Di2.60.20.30. 1 hit.
InterProiIPR015791. Antimic/Inh_G_crystallin-like.
IPR011024. G_crystallin-rel.
IPR007141. Prot_inh_SMPI.
[Graphical view]
PfamiPF03995. Inhibitor_I36. 1 hit.
[Graphical view]
SUPFAMiSSF49695. SSF49695. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P01077-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MVRKRALGLA GSALTLVLGA VGFTAPAQAA PSCPAGSLCT YSGTGLSGAR
60 70 80 90 100
TVIPASDMEK AGTDGVKLPA SARSFANGTH FTLRYGPARK VTCVRFPCYQ
110 120 130
YATVGKVAPG AQLRSLPSPG ATVTVGQDLG D
Length:131
Mass (Da):13,186
Last modified:August 1, 1992 - v2
Checksum:iD204C23A57A67BD1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D00671 Genomic DNA. Translation: BAA00574.1.
PIRiS12615. ZYSMN.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D00671 Genomic DNA. Translation: BAA00574.1.
PIRiS12615. ZYSMN.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1BHUNMR-A30-131[»]
ProteinModelPortaliP01077.
SMRiP01077. Positions 30-131.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiI36.001.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP01077.

Family and domain databases

Gene3Di2.60.20.30. 1 hit.
InterProiIPR015791. Antimic/Inh_G_crystallin-like.
IPR011024. G_crystallin-rel.
IPR007141. Prot_inh_SMPI.
[Graphical view]
PfamiPF03995. Inhibitor_I36. 1 hit.
[Graphical view]
SUPFAMiSSF49695. SSF49695. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Nucleotide sequence of the gene for a metalloproteinase inhibitor of Streptomyces nigrescens (SMPI)."
    Tanaka K., Aoki H., Oda K., Murao S., Saito H., Takahashi H.
    Nucleic Acids Res. 18:6433-6433(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Amino acid sequence of Streptomyces metallo-proteinase inhibitor from Streptomyces nigrescens TK-23."
    Murai H., Hara S., Ikenaka T., Oda K., Murao S.
    J. Biochem. 97:173-180(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 30-131, DISULFIDE BONDS.
    Strain: TK-23.
  3. "NMR structure of the Streptomyces metalloproteinase inhibitor, SMPI, isolated from Streptomyces nigrescens TK-23: another example of an ancestral beta gamma-crystallin precursor structure."
    Ohno A., Tate S., Seeram S.S., Hiraga K., Swindells M.B., Oda K., Kainosho M.
    J. Mol. Biol. 282:421-433(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR.
    Strain: TK-23.

Entry informationi

Entry nameiIMEP_STRNI
AccessioniPrimary (citable) accession number: P01077
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: August 1, 1992
Last modified: November 26, 2014
This is version 67 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Thermolysin slowly cleaves only the peptide bond following Cys-93.

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.